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Aliases for CASP3 Gene

Aliases for CASP3 Gene

  • Caspase 3, Apoptosis-Related Cysteine Peptidase 2 3
  • CPP32 3 4 6
  • Caspase 3, Apoptosis-Related Cysteine Protease 2 3
  • SREBP Cleavage Activity 1 3 4
  • Cysteine Protease CPP32 3 4
  • Protein Yama 3 4
  • EC 3.4.22.56 4 64
  • Apopain 3 4
  • CASP-3 3 4
  • CPP-32 3 4
  • SCA-1 3 4
  • PARP Cleavage Protease 3
  • Procaspase3 3
  • Caspase-3 3
  • EC 3.4.22 64
  • CPP32B 3

External Ids for CASP3 Gene

Summaries for CASP3 Gene

Entrez Gene Summary for CASP3 Gene

  • This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]

GeneCards Summary for CASP3 Gene

CASP3 (Caspase 3, Apoptosis-Related Cysteine Peptidase) is a Protein Coding gene. Diseases associated with CASP3 include nervous system cancer and myocardial infarction. Among its related pathways are Signaling by FGFR and MAPK signaling pathway. GO annotations related to this gene include peptidase activity and cyclin-dependent protein serine/threonine kinase inhibitor activity. An important paralog of this gene is CASP1.

UniProtKB/Swiss-Prot for CASP3 Gene

  • Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a 216-Asp- -Gly-217 bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.

Tocris Summary for CASP3 Gene

  • Caspases (short for cysteinyl aspartate proteases) are involved in the signal transduction pathways of apoptosis, necrosis and inflammation. These enzymes can be divided into two major classes - initiators and effectors. The initiator isoforms (caspases-1,-4,-5,-8,-9,-10,-11,-12) are activated by, and interact with, upstream adaptor molecules through protein-protein interaction domains known as CARD and DED. Effector caspases (-3,-6,-7) are responsible for cleaving downstream substrates and are sometimes referred to as the executioner caspases. More than 400 caspase substrates have so far been identified (see The Caspase Substrate Database). Initiator caspases, such as caspase 8, may be directly activated by death receptors such as FasR. Caspases can also be found intracellularly as part of large multiprotein complexes. For example, caspase 9 is recruited to the apoptosome formed during apoptosis, whilst caspases-1 and 5 can form part of the inflammasome, a key part of cytokine processing during inflammation. Caspases are regulated by inhibitors of apoptosis and by dominant negative isoforms. They have been implicated in the pathogenesis of many disorders including stroke, Alzheimers disease, myocardial infarction, cancer, and inflammatory disease.

Gene Wiki entry for CASP3 Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CASP3 Gene

Genomics for CASP3 Gene

Genomic Location for CASP3 Gene

Start:
184,627,696 bp from pter
End:
184,649,509 bp from pter
Size:
21,814 bases
Orientation:
Minus strand

Genomic View for CASP3 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for CASP3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CASP3 Gene

Regulatory Elements for CASP3 Gene

Proteins for CASP3 Gene

  • Protein details for CASP3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P42574-CASP3_HUMAN
    Recommended name:
    Caspase-3
    Protein Accession:
    P42574
    Secondary Accessions:
    • A8K5M2
    • D3DP53
    • Q96AN1
    • Q96KP2

    Protein attributes for CASP3 Gene

    Size:
    277 amino acids
    Molecular mass:
    31608 Da
    Quaternary structure:
    • Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce.

    Three dimensional structures from OCA and Proteopedia for CASP3 Gene

neXtProt entry for CASP3 Gene

Proteomics data for CASP3 Gene at MOPED

Post-translational modifications for CASP3 Gene

  • Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa
  • S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • Modification sites at PhosphoSitePlus
  • Ubiquitination at Lys229

Other Protein References for CASP3 Gene

Domains for CASP3 Gene

Gene Families for CASP3 Gene

HGNC:
IUPHAR :

UniProtKB/Swiss-Prot:

CASP3_HUMAN
Family:
  • Belongs to the peptidase C14A family.:
    • P42574
genes like me logo Genes that share domains with CASP3: view

Function for CASP3 Gene

Molecular function for CASP3 Gene

GENATLAS Biochemistry: caspase 3,effector,cysteine containing aspartate-specific protease CPP32,activated by CASP9,cleaving specifically CDKN1A,alpha-fodrin (SPTAN1), gelsolin (GSN),stored in the mitochondrial intermembrane space and released into cytosol after appropriate apoptotic stimuli,promoting CD95/APO1/fas mediated apoptosis,homolog to C elegans Ced-3 and mammalian interleukin 1 beta convertase (IL1BC),including two isoforms alpha and beta
UniProtKB/Swiss-Prot CatalyticActivity: Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp- - with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position
UniProtKB/Swiss-Prot EnzymeRegulation: Inhibited by isatin sulfonamides
UniProtKB/Swiss-Prot Function: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a 216-Asp- -Gly-217 bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.

Enzyme Numbers (IUBMB) for CASP3 Gene

Gene Ontology (GO) - Molecular Function for CASP3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004190 aspartic-type endopeptidase activity EXP --
GO:0004197 cysteine-type endopeptidase activity TAS --
GO:0004861 cyclin-dependent protein serine/threonine kinase inhibitor activity IEA --
GO:0005515 protein binding IPI 11257232
GO:0008233 peptidase activity IDA 17167422
genes like me logo Genes that share ontologies with CASP3: view
genes like me logo Genes that share phenotypes with CASP3: view

Animal Models for CASP3 Gene

MGI Knock Outs for CASP3:

No data available for Transcription Factor Targeting and HOMER Transcription for CASP3 Gene

Localization for CASP3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CASP3 Gene

Cytoplasm.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for CASP3 Gene COMPARTMENTS Subcellular localization image for CASP3 gene
Compartment Confidence
cytosol 5
mitochondrion 4
nucleus 4
plasma membrane 4
cytoskeleton 3
endoplasmic reticulum 3
extracellular 3
lysosome 2
vacuole 2
endosome 1
golgi apparatus 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for CASP3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA 17167422
GO:0005654 nucleoplasm TAS --
GO:0005737 cytoplasm --
GO:0005829 cytosol TAS --
GO:0005886 plasma membrane TAS --
genes like me logo Genes that share ontologies with CASP3: view

Pathways for CASP3 Gene

genes like me logo Genes that share pathways with CASP3: view

Gene Ontology (GO) - Biological Process for CASP3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001782 B cell homeostasis IEA --
GO:0001836 release of cytochrome c from mitochondria IEA --
GO:0006309 apoptotic DNA fragmentation TAS --
GO:0006508 proteolysis IDA 12888622
GO:0006915 apoptotic process TAS --
genes like me logo Genes that share ontologies with CASP3: view

Compounds for CASP3 Gene

(12) ApexBio Compounds for CASP3 Gene

Compound Action Cas Number
Apoptosis Inhibitor Cell-permeable caspase-3 inhibitor [54135-60-3]
Caspase-3/7 Inhibitor I Cell-permeable and irreversible inhibitor of caspase-3 and -7 [220509-74-0]
Cisplatin Chemotherapy agent, induces apoptosis [15663-27-1]
PAC-1 Procaspase-activating compound, activates procaspase-3 and converts it to caspase-3 [315183-21-2]
Q-VD-Oph Cell-permeable, irreversible pan-caspase inhibitor [1135695-98-5]
VX-765 Novel Caspase-1 inhibitor [273404-37-8]
Z-DEVD-FMK Cell-permeable, and irreversible caspase-3 inhibitor [210344-95-9]
Z-DQMD-FMK Cell-permeable caspase-3 inhibitor
Z-FA-FMK An inhibitor of cysteine proteases, such as cathepsin B.
Z-VAD-FMK Cell-permeable, irreversible pan-caspase inhibitor [187389-52-2]
Z-VDVAD-FMK Cell-permeable and irreversible caspase-2 inhibitor
Z-VEID-FMK Cell-permeable and irreversible caspase-6 inhibitor

(5) Tocris Compounds for CASP3 Gene

Compound Action Cas Number
AZ 10417808 Selective non-peptide caspase-3 inhibitor [331645-84-2]
Cisplatin Potent pro-apoptotic anticancer agent; activates caspase-3 [15663-27-1]
Z-DEVD-FMK Cell-permeable, irreversible caspase-3 inhibitor
Z-DQMD-FMK Caspase-3 inhibitor
Z-VAD-FMK Cell-permeable, irreversible caspase inhibitor [187389-52-2]

(2) HMDB Compounds for CASP3 Gene

Compound Synonyms Cas Number PubMed IDs
Butyric acid
  • 1-Butyrate
107-92-6
Simvastatin
  • (+)-Simvastatin
79902-63-9

(5) IUPHAR Ligand for CASP3 Gene

Ligand Type Action Affinity Pubmed IDs
PETCM Activator None
AZ10417808 Inhibitor Inhibition
Z-DEVD-FMK Inhibitor Inhibition
Z-DQMD-FMK Inhibitor Inhibition
PAC1 Activator None

(130) Novoseek inferred chemical compound relationships for CASP3 Gene

Compound -log(P) Hits PubMed IDs
adpribose 87.7 107
phosphatidylserine 82.2 120
dutp 81.3 71
sp 600125 77.4 28
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide 77 15
genes like me logo Genes that share compounds with CASP3: view

Transcripts for CASP3 Gene

Unigene Clusters for CASP3 Gene

Caspase 3, apoptosis-related cysteine peptidase:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for CASP3 Gene

ExUns: 1a · 1b ^ 2a · 2b · 2c ^ 3 ^ 4 ^ 5 ^ 6a · 6b · 6c ^ 7 ^ 8a · 8b
SP1: -
SP2: - - -
SP3: -
SP4: -

Relevant External Links for CASP3 Gene

GeneLoc Exon Structure for
CASP3
ECgene alternative splicing isoforms for
CASP3

Expression for CASP3 Gene

mRNA expression in normal human tissues for CASP3 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for CASP3 Gene

SOURCE GeneReport for Unigene cluster for CASP3 Gene Hs.141125

mRNA Expression by UniProt/SwissProt for CASP3 Gene

P42574-CASP3_HUMAN
Tissue specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system
genes like me logo Genes that share expressions with CASP3: view

Orthologs for CASP3 Gene

This gene was present in the common ancestor of animals.

Orthologs for CASP3 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia CASP3 36
  • 99.52 (n)
  • 99.64 (a)
CASP3 37
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia CASP3 36
  • 86.3 (n)
  • 84 (a)
CASP3 37
  • 84 (a)
OneToOne
dog
(Canis familiaris)
Mammalia CASP3 36
  • 89.53 (n)
  • 89.17 (a)
CASP3 37
  • 82 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Casp3 36
  • 83.63 (n)
  • 86.64 (a)
Casp3 16
Casp3 37
  • 87 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia -- 37
  • 78 (a)
OneToMany
-- 37
  • 71 (a)
OneToMany
-- 37
  • 72 (a)
OneToMany
platypus
(Ornithorhynchus anatinus)
Mammalia CASP3 37
  • 68 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Casp3 36
  • 84.84 (n)
  • 89.17 (a)
chicken
(Gallus gallus)
Aves CASP3 36
  • 70.76 (n)
  • 66.91 (a)
CASP3 37
  • 64 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia casp3 36
  • 66.67 (n)
  • 63.07 (a)
zebrafish
(Danio rerio)
Actinopterygii casp3 36
casp3a 37
  • 55 (a)
OneToMany
casp3b 37
  • 53 (a)
OneToMany
African malaria mosquito
(Anopheles gambiae)
Insecta CASPS8 36
  • 45.94 (n)
  • 37.08 (a)
fruit fly
(Drosophila melanogaster)
Insecta Dcp-1 37
  • 30 (a)
ManyToMany
decay 37
  • 30 (a)
ManyToMany
Ice 37
  • 32 (a)
ManyToMany
Dcp-1 38
  • 39 (a)
Ice 38
  • 42 (a)
decay 38
  • 37 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 37
  • 39 (a)
ManyToMany
-- 37
  • 33 (a)
ManyToMany
-- 37
  • 38 (a)
ManyToMany
-- 37
  • 36 (a)
ManyToMany
-- 37
  • 40 (a)
ManyToMany
-- 37
  • 34 (a)
ManyToMany
-- 37
  • 32 (a)
ManyToMany
-- 37
  • 39 (a)
ManyToMany
-- 37
  • 23 (a)
ManyToMany
-- 37
  • 47 (a)
ManyToMany
Species with no ortholog for CASP3:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for CASP3 Gene

ENSEMBL:
Gene Tree for CASP3 (if available)
TreeFam:
Gene Tree for CASP3 (if available)

Paralogs for CASP3 Gene

Selected SIMAP similar genes for CASP3 Gene using alignment to 3 proteins:

Pseudogenes.org Pseudogenes for CASP3 Gene

genes like me logo Genes that share paralogs with CASP3: view

Variants for CASP3 Gene

Sequence variations from dbSNP and Humsavar for CASP3 Gene

SNP ID Clin Chr 04 pos Sequence Context AA Info Type MAF
rs6948 -- 184,627,976(-) TGAGC(A/C)CTCAG utr-variant-3-prime
rs8549 -- 184,627,854(-) CTCGC(A/G)TTTGT utr-variant-3-prime
rs1049210 - 184,631,071(-) GTGGA(G/T)GCCGA reference, missense
rs1049216 -- 184,628,935(-) AGTAA(C/T)GAATT utr-variant-3-prime
rs1049253 -- 184,627,797(-) ACACA(C/T)AGCAT utr-variant-3-prime

Structural Variations from Database of Genomic Variants (DGV) for CASP3 Gene

Variant ID Type Subtype PubMed ID
nsv830168 CNV Loss 17160897

Relevant External Links for CASP3 Gene

HapMap Linkage Disequilibrium report
CASP3
Human Gene Mutation Database (HGMD)
CASP3

Disorders for CASP3 Gene

(93) Novoseek inferred disease relationships for CASP3 Gene

Disease -log(P) Hits PubMed IDs
necrosis 77.4 118
tumors 75.6 166
cancer 75.5 113
leukemia 75.1 115
promyelocytic leukemia 71.1 10

Relevant External Links for CASP3

Genetic Association Database (GAD)
CASP3
Human Genome Epidemiology (HuGE) Navigator
CASP3
genes like me logo Genes that share disorders with CASP3: view

No data available for UniProtKB/Swiss-Prot for CASP3 Gene

Publications for CASP3 Gene

  1. Cleavage of huntingtin by apopain, a proapoptotic cysteine protease, is modulated by the polyglutamine tract. (PMID: 8696339) Goldberg Y.P. … Hayden M.R. (Nat. Genet. 1996) 3 4 23
  2. Structure of recombinant human CPP32 in complex with the tetrapeptide acetyl-Asp-Val-Ala-Asp fluoromethyl ketone. (PMID: 9045680) Mittl P.R.E. … Gruetter M.G. (J. Biol. Chem. 1997) 3 4 23
  3. CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme. (PMID: 7983002) Fernandes-Alnemri T. … Alnemri E.S. (J. Biol. Chem. 1994) 3 4 23
  4. Identification of polymorphisms in the Caspase-3 gene and their association with lung cancer risk. (PMID: 18058802) Jang J.S. … Park J.Y. (Mol. Carcinog. 2008) 3 23 49
  5. Genistein sensitizes TRAIL-resistant human gastric adenocarcinoma AGS cells through activation of caspase-3. (PMID: 17689858) Jin C.Y. … Choi Y.H. (Cancer Lett. 2007) 3 23

Products for CASP3 Gene

  • Addgene plasmids for CASP3

Sources for CASP3 Gene

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