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Aliases for NOX5 Gene

Aliases for NOX5 Gene

  • NADPH Oxidase, EF-Hand Calcium Binding Domain 5 2 3
  • EC 1.6.3.- 4

External Ids for NOX5 Gene

Previous GeneCards Identifiers for NOX5 Gene

  • GC15P065065
  • GC15P062322
  • GC15P066801
  • GC15P066939
  • GC15P066940
  • GC15P067009
  • GC15P069222
  • GC15P046055

Summaries for NOX5 Gene

Entrez Gene Summary for NOX5 Gene

  • This gene is predominantly expressed in the testis and lymphocyte-rich areas of spleen and lymph nodes. It encodes a calcium-dependen NADPH oxidase that generates superoxide, and functions as a calcium-dependent proton channel that may regulate redox-dependent processes in lymphocytes and spermatozoa. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]

GeneCards Summary for NOX5 Gene

NOX5 (NADPH Oxidase, EF-Hand Calcium Binding Domain 5) is a Protein Coding gene. Among its related pathways are PAK Pathway and Apoptotic Pathways in Synovial Fibroblasts. GO annotations related to this gene include calcium ion binding and flavin adenine dinucleotide binding. An important paralog of this gene is NOX4.

UniProtKB/Swiss-Prot for NOX5 Gene

  • Calcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. May play a role in cell growth and apoptosis. Isoform v2 and isoform v5 are involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin.

Gene Wiki entry for NOX5 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NOX5 Gene

Genomics for NOX5 Gene

Regulatory Elements for NOX5 Gene

Genomic Location for NOX5 Gene

Start:
68,930,500 bp from pter
End:
69,062,743 bp from pter
Size:
132,244 bases
Orientation:
Plus strand

Genomic View for NOX5 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for NOX5 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NOX5 Gene

Proteins for NOX5 Gene

  • Protein details for NOX5 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q96PH1-NOX5_HUMAN
    Recommended name:
    NADPH oxidase 5
    Protein Accession:
    Q96PH1
    Secondary Accessions:
    • B2RBJ4
    • Q08AN2
    • Q08AN3
    • Q8TEQ1
    • Q8TER4
    • Q96PH2
    • Q96PJ8
    • Q96PJ9
    • Q9H6E0
    • Q9HAM8

    Protein attributes for NOX5 Gene

    Size:
    765 amino acids
    Molecular mass:
    86439 Da
    Cofactor:
    Name=FAD; Xref=ChEBI:CHEBI:57692;
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    No Data Available
    SequenceCaution:
    • Sequence=AAG33638.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=BAB15319.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=BAB84897.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};

    Alternative splice isoforms for NOX5 Gene

neXtProt entry for NOX5 Gene

Proteomics data for NOX5 Gene at MOPED

Post-translational modifications for NOX5 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for NOX5 Gene

Domains for NOX5 Gene

Gene Families for NOX5 Gene

HGNC:
  • EFHAND :EF-hand domain containing

Suggested Antigen Peptide Sequences for NOX5 Gene

Graphical View of Domain Structure for InterPro Entry

Q96PH1

UniProtKB/Swiss-Prot:

NOX5_HUMAN :
  • Q96PH1
Domain:
  • Isoform v1 and isoform v2 of this protein have four functional EF-hand calcium-binding domains. Isoform v3 and isoform v4 have the third EF-hand domain interrupted by an insert
  • Contains 4 EF-hand domains.
  • Contains 1 FAD-binding FR-type domain.
  • Contains 1 ferric oxidoreductase domain.
genes like me logo Genes that share domains with NOX5: view

Function for NOX5 Gene

Molecular function for NOX5 Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties: Kinetic parameters: KM=1.06 uM for calcium {ECO:0000269 PubMed:14982937};
UniProtKB/Swiss-Prot EnzymeRegulation: Activated by calcium which induces conformational changes and interaction between the N-terminal regulatory region and the C-terminal catalytic region. Inhibited by diphenylene iodonium.
UniProtKB/Swiss-Prot Function: Calcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. May play a role in cell growth and apoptosis. Isoform v2 and isoform v5 are involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin.
UniProtKB/Swiss-Prot Induction: Down-regulated by TGFB1.

Enzyme Numbers (IUBMB) for NOX5 Gene

Gene Ontology (GO) - Molecular Function for NOX5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005509 calcium ion binding IEA --
GO:0005515 protein binding IPI 17346712
GO:0015252 hydrogen ion channel activity IDA 11483596
GO:0016175 superoxide-generating NADPH oxidase activity IDA 11483596
GO:0016491 oxidoreductase activity --
genes like me logo Genes that share ontologies with NOX5: view

Phenotypes for NOX5 Gene

GenomeRNAi human phenotypes for NOX5:
genes like me logo Genes that share phenotypes with NOX5: view

No data available for Animal Models , miRNA , Transcription Factor Targeting and HOMER Transcription for NOX5 Gene

Localization for NOX5 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NOX5 Gene

Membrane; Multi-pass membrane protein.
Isoform v2: Endoplasmic reticulum.
Isoform v5: Endoplasmic reticulum.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for NOX5 Gene COMPARTMENTS Subcellular localization image for NOX5 gene
Compartment Confidence
endoplasmic reticulum 5
plasma membrane 3
peroxisome 2

Gene Ontology (GO) - Cellular Components for NOX5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005783 endoplasmic reticulum IEA --
GO:0016021 integral component of membrane IEA --
genes like me logo Genes that share ontologies with NOX5: view

Pathways for NOX5 Gene

genes like me logo Genes that share pathways with NOX5: view

Pathways by source for NOX5 Gene

2 Qiagen pathways for NOX5 Gene

Interacting Proteins for NOX5 Gene

Gene Ontology (GO) - Biological Process for NOX5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000910 cytokinesis NAS 11483596
GO:0001525 angiogenesis IEA --
GO:0001935 endothelial cell proliferation IDA 17275676
GO:0006915 apoptotic process NAS 11483596
GO:0008283 cell proliferation NAS 11483596
genes like me logo Genes that share ontologies with NOX5: view

Compounds for NOX5 Gene

(5) HMDB Compounds for NOX5 Gene

Compound Synonyms Cas Number PubMed IDs
Calcium
  • Ca
7440-70-2
FAD
  • 1H-Purin-6-amine flavin dinucleotide
146-14-5
Heme
  • (protoporphyrinato)iron
14875-96-8
Magnesium
  • Magnesium
7439-95-4
NADPH
  • 2'-(dihydrogen phosphate) 5'-(trihydrogen pyrophosphate) Adenosine 5'-ester with 1,4-dihydro-1-b-D-ribofuranosylnicotinamide
53-57-6

(2) Novoseek inferred chemical compound relationships for NOX5 Gene

Compound -log(P) Hits PubMed IDs
nadph 66.3 10
superoxide 49.8 6
genes like me logo Genes that share compounds with NOX5: view

Transcripts for NOX5 Gene

Unigene Clusters for NOX5 Gene

NADPH oxidase, EF-hand calcium binding domain 5:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for NOX5 Gene

No ASD Table

Relevant External Links for NOX5 Gene

GeneLoc Exon Structure for
NOX5
ECgene alternative splicing isoforms for
NOX5

Expression for NOX5 Gene

mRNA expression in normal human tissues for NOX5 Gene

mRNA differential expression in normal tissues according to GTEx for NOX5 Gene

This gene is overexpressed in Spleen (14.8) and Testis (9.1).

Integrated Proteomics: protein expression from ProteomicsDB and MOPED for NOX5 Gene

SOURCE GeneReport for Unigene cluster for NOX5 Gene Hs.657932

mRNA Expression by UniProt/SwissProt for NOX5 Gene

Q96PH1-NOX5_HUMAN
Tissue specificity: Mainly expressed in pachytene spermatocytes of testis and in lymphocyte-rich areas of spleen and lymph nodes. Isoform v1 is expressed in spleen. Isoform v2 is expressed in testis. Also detected in ovary, placenta, pancreas, cardiac fibroblasts. Expressed in B-cells and prostate malignant cells. Isoform v1 and isoform v3 are expressed in epithelial colorectal adenocarcinoma cells. Isoform v2 and isoform v4 are expressed in endothelial cells. Isoform v1, isoform v2, isoform v3 and isoform v4 are expressed in pulmonary artery smooth muscle cells. Isoform v2 and isoform v5 are expressed in microvascular endothelial cells (at protein level).
genes like me logo Genes that share expressions with NOX5: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery for NOX5 Gene

Orthologs for NOX5 Gene

This gene was present in the common ancestor of animals.

Orthologs for NOX5 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia NOX5 35
  • 99.46 (n)
  • 99.59 (a)
NOX5 36
  • 99 (a)
OneToOne
cow
(Bos Taurus)
Mammalia NOX5 35
  • 86.79 (n)
  • 86.23 (a)
NOX5 36
  • 83 (a)
OneToOne
dog
(Canis familiaris)
Mammalia NOX5 35
  • 86.32 (n)
  • 85.26 (a)
NOX5 36
  • 84 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia NOX5 36
  • 73 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia NOX5 36
  • 70 (a)
OneToOne
chicken
(Gallus gallus)
Aves NOX5 35
  • 69.84 (n)
  • 63.78 (a)
NOX5 36
  • 61 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia NOX5 36
  • 57 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia nox5 35
  • 68.15 (n)
  • 68.71 (a)
zebrafish
(Danio rerio)
Actinopterygii nox5 35
  • 69.13 (n)
  • 68.58 (a)
NOX5 36
  • 66 (a)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP008072 35
  • 59.51 (n)
  • 52.15 (a)
fruit fly
(Drosophila melanogaster)
Insecta Nox 35
  • 56.89 (n)
  • 50.07 (a)
Nox 36
  • 27 (a)
OneToOne
CG3896 37
  • 41 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 12 (a)
ManyToMany
Species with no ortholog for NOX5:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • mouse (Mus musculus)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rat (Rattus norvegicus)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for NOX5 Gene

ENSEMBL:
Gene Tree for NOX5 (if available)
TreeFam:
Gene Tree for NOX5 (if available)

Paralogs for NOX5 Gene

Paralogs for NOX5 Gene

genes like me logo Genes that share paralogs with NOX5: view

Variants for NOX5 Gene

Sequence variations from dbSNP and Humsavar for NOX5 Gene

SNP ID Clin Chr 15 pos Sequence Context AA Info Type MAF
rs167482 -- 69,015,953(+) GGCCC(A/G)GGACA intron-variant
rs167484 -- 69,011,810(+) CCTTC(C/T)GCATG intron-variant
rs178139 -- 69,033,667(-) TCTTA(A/G)AGGCC intron-variant
rs178140 -- 69,039,779(-) GACAG(C/T)TGAGC intron-variant
rs186349 -- 69,051,093(-) aggtg(A/G)gaagg intron-variant

Structural Variations from Database of Genomic Variants (DGV) for NOX5 Gene

Variant ID Type Subtype PubMed ID
nsv1584 CNV Loss 18451855
esv33141 CNV Gain 17666407
esv33333 CNV Gain 17666407
esv33369 CNV Gain 17666407
esv2669530 CNV Deletion 23128226
nsv521203 CNV Loss 19592680
esv275002 CNV Gain+Loss 21479260

Relevant External Links for NOX5 Gene

HapMap Linkage Disequilibrium report
NOX5

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NOX5 Gene

Disorders for NOX5 Gene

Relevant External Links for NOX5

Genetic Association Database (GAD)
NOX5
Human Genome Epidemiology (HuGE) Navigator
NOX5
genes like me logo Genes that share disorders with NOX5: view

No data available for UniProtKB/Swiss-Prot for NOX5 Gene

Publications for NOX5 Gene

  1. A Ca(2+)-activated NADPH oxidase in testis, spleen, and lymph nodes. (PMID: 11483596) Banfi B. … Krause K.-H. (J. Biol. Chem. 2001) 2 3 4 23
  2. Mechanism of Ca2+ activation of the NADPH oxidase 5 (NOX5). (PMID: 14982937) Banfi B. … Cox J.A. (J. Biol. Chem. 2004) 3 4 23
  3. NADPH oxidase 5 (NOX5) interacts with and is regulated by calmodulin. (PMID: 17346712) Tirone F. … Cox J.A. (FEBS Lett. 2007) 3 23
  4. Homologs of gp91phox: cloning and tissue expression of Nox3, Nox4, and Nox5. (PMID: 11376945) Cheng G. … Lambeth J.D. (Gene 2001) 3 4
  5. NAD(P)H oxidase 4 mediates transforming growth factor-beta1-induced differentiation of cardiac fibroblasts into myofibroblasts. (PMID: 16179589) Cucoranu I. … Sorescu D. (Circ. Res. 2005) 3 4

Products for NOX5 Gene

  • Pathway & Disease-focused RT² Profiler PCR Arrays
    • Apoptosis 384HT in human,mouse,rat
    • Inflammatory Response & Autoimmunity 384HT in human,mouse,rat
    • Nitric Oxide Signaling Pathway in human,mouse,rat
    • Oxidative Stress in human,mouse,rat
  • QIAGEN qRT-PCR Assays for microRNAs that regulate NOX5
  • QuantiTect SYBR Green Assays in human,mouse,rat
  • Pre-validated RT² qPCR Primer Assay in human,mouse,rat
  • QuantiFast Probe-based Assays in human,mouse,rat
  • Predesigned siRNA for gene silencing in human,mouse,rat for NOX5
  • Block miRNA regulation of NOX5 using miScript Target Protectors

Sources for NOX5 Gene

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