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Aliases for CA9 Gene

Aliases for CA9 Gene

  • Carbonic Anhydrase IX 2 3 4
  • MN 3 4 6
  • Renal Cell Carcinoma-Associated Antigen G250 3 4
  • RCC-Associated Protein G250 2 3
  • RCC-Associated Antigen G250 3 4
  • Carbonate Dehydratase IX 3 4
  • Carbonic Dehydratase 2 3
  • Membrane Antigen MN 3 4
  • P54/58N 3 4
  • CA-IX 3 4
  • CAIX 3 4
  • PMW1 3 4
  • Carbonic Anhydrase 9 3
  • EC 4
  • G250 4

External Ids for CA9 Gene

Previous GeneCards Identifiers for CA9 Gene

  • GC09P035986
  • GC09P035843
  • GC09P035663
  • GC09P035629

Summaries for CA9 Gene

Entrez Gene Summary for CA9 Gene

  • Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]

GeneCards Summary for CA9 Gene

CA9 (Carbonic Anhydrase IX) is a Protein Coding gene. Diseases associated with CA9 include clear cell renal cell carcinoma and oxyphilic adenoma. Among its related pathways are Angiogenesis (CST) and Metabolism. GO annotations related to this gene include carbonate dehydratase activity. An important paralog of this gene is CA14.

UniProtKB/Swiss-Prot for CA9 Gene

  • Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.

Tocris Summary for CA9 Gene

  • Carbonic anhydrases are zinc-containing enzymes belonging to the lyases family, and are primarily responsible for catalyzing the reversible hydration of carbon dioxide to form bicarbonate (HCO3-) and hydrogen (H+) ions. There are several different carbonic anhydrase isoforms; these can be broadly grouped into cytosolic (CA I, CA II, CA III, CA VII, CA XIII), mitochondrial (CA VA, CA VB), secreted (CA VI) and membrane-associated (CA IV, CA IX, CA XII, CA XIV) carbonic anhydrases. The functions of the remaining carbonic anhydrases are yet to be elucidated. Expression of the membrane-bound carbonic anhydrase, CA IX, has been reported in hypoxic tumors where its activity is linked to promoting survival of tumor cells and aiding cancer progression. This is thought to occur through the carbonic anhyrase-mediated production of bicarbonate ions, which neutralizes the abnormally acidic pH of the extracellular environment induced during hypoxia. Furthermore, as CA IX is a hypoxia- and HIF-1-inducible enzyme, its expression can be used as a biomarker for hypoxic tumors.

Gene Wiki entry for CA9 Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CA9 Gene

Genomics for CA9 Gene

Regulatory Elements for CA9 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for CA9 Gene

35,673,856 bp from pter
35,681,159 bp from pter
7,304 bases
Plus strand

Genomic View for CA9 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for CA9 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CA9 Gene

Proteins for CA9 Gene

  • Protein details for CA9 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Carbonic anhydrase 9
    Protein Accession:
    Secondary Accessions:
    • Q5T4R1

    Protein attributes for CA9 Gene

    459 amino acids
    Molecular mass:
    49698 Da
    Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
    Quaternary structure:
    • Forms oligomers linked by disulfide bonds.

    Three dimensional structures from OCA and Proteopedia for CA9 Gene

neXtProt entry for CA9 Gene

Proteomics data for CA9 Gene at MOPED

Post-translational modifications for CA9 Gene

Other Protein References for CA9 Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

Antibody Products

  • R&D Systems Antibodies for CA9 (Carbonic Anhydrase IX/CA9)
  • Cell Signaling Technology (CST) Antibodies for CA9 (CA9)

Protein Products

No data available for DME Specific Peptides for CA9 Gene

Domains for CA9 Gene

Gene Families for CA9 Gene

  • CA :Carbonic anhydrases

Suggested Antigen Peptide Sequences for CA9 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry



  • Q16790
  • Belongs to the alpha-carbonic anhydrase family.
genes like me logo Genes that share domains with CA9: view

Function for CA9 Gene

Molecular function for CA9 Gene

GENATLAS Biochemistry: carbonic anhydrase 9,MN/CA antigen expressed in Hela cells and some human carcinomas
UniProtKB/Swiss-Prot BiophysicochemicalProperties: pH dependence: Optimum pH is 6.5. {ECO:0000269 PubMed:19805286};
UniProtKB/Swiss-Prot CatalyticActivity: H(2)CO(3) = CO(2) + H(2)O
UniProtKB/Swiss-Prot EnzymeRegulation: Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA) and Foscarnet (phosphonoformate trisodium salt).
UniProtKB/Swiss-Prot Function: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
UniProtKB/Swiss-Prot Induction: By hypoxia.

Enzyme Numbers (IUBMB) for CA9 Gene

Gene Ontology (GO) - Molecular Function for CA9 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004089 carbonate dehydratase activity IEA --
GO:0008270 zinc ion binding IEA --
genes like me logo Genes that share ontologies with CA9: view
genes like me logo Genes that share phenotypes with CA9: view

Animal Models for CA9 Gene

MGI Knock Outs for CA9:

Animal Model Products

CRISPR Products

miRNA for CA9 Gene

miRTarBase miRNAs that target CA9

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for CA9

In Situ Assay Products

Flow Cytometry Products

No data available for Transcription Factor Targeting and HOMER Transcription for CA9 Gene

Localization for CA9 Gene

Subcellular locations from UniProtKB/Swiss-Prot for CA9 Gene

Nucleus. Nucleus, nucleolus. Cell membrane; Single-pass type I membrane protein. Cell projection, microvillus membrane; Single-pass type I membrane protein. Note=Found on the surface microvilli and in the nucleus, particularly in nucleolus.

Subcellular locations from

Jensen Localization Image for CA9 Gene COMPARTMENTS Subcellular localization image for CA9 gene
Compartment Confidence
nucleus 5
plasma membrane 5
extracellular 2
mitochondrion 2
chloroplast 1
cytoskeleton 1
cytosol 1
endoplasmic reticulum 1

Gene Ontology (GO) - Cellular Components for CA9 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus --
GO:0005730 nucleolus IEA --
GO:0005886 plasma membrane TAS --
GO:0016021 integral component of membrane IEA --
GO:0016323 basolateral plasma membrane IEA --
genes like me logo Genes that share ontologies with CA9: view

Pathways for CA9 Gene

genes like me logo Genes that share pathways with CA9: view

Pathways by source for CA9 Gene

PCR Array Products

Gene Ontology (GO) - Biological Process for CA9 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001666 response to hypoxia --
GO:0002009 morphogenesis of an epithelium IEA --
GO:0006730 one-carbon metabolic process IEA --
GO:0015701 bicarbonate transport TAS --
GO:0033574 response to testosterone IEA --
genes like me logo Genes that share ontologies with CA9: view

Compounds for CA9 Gene

(1) Tocris Compounds for CA9 Gene

Compound Action Cas Number
U 104 Potent carbonic anhydrase (CA) IX and XII inhibitor [178606-66-1]

(4) HMDB Compounds for CA9 Gene

Compound Synonyms Cas Number PubMed IDs
Carbon dioxide
  • Carbon oxide
Carbonic acid
  • acid of air
  • Dihydrogen oxide
  • Zinc

(5) Drugbank Compounds for CA9 Gene

Compound Synonyms Cas Number Type Actions PubMed IDs
91-33-8 target inhibitor
Ellagic Acid
476-66-4 target inhibitor
  • Dihydrochlorothiazid
58-93-5 target inhibitor
  • Dihydroflumethazide
135-09-1 target inhibitor
  • Zonisamida [Spanish]
68291-97-4 target inhibitor

(33) Novoseek inferred chemical compound relationships for CA9 Gene

Compound -log(P) Hits PubMed IDs
pimonidazole 88.2 43
dichlorphenamide 70.4 2
vegf 65.8 94
ethoxzolamide 65.3 2
methazolamide 63.6 2
genes like me logo Genes that share compounds with CA9: view

Transcripts for CA9 Gene

Unigene Clusters for CA9 Gene

Carbonic anhydrase IX:
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for CA9

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for CA9 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4a · 4b · 4c ^ 5 ^ 6 ^ 7 ^ 8a · 8b ^ 9 ^ 10 ^ 11
SP2: -
SP3: -
SP4: -

Relevant External Links for CA9 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for CA9 Gene

mRNA expression in normal human tissues for CA9 Gene

mRNA differential expression in normal tissues according to GTEx for CA9 Gene

This gene is overexpressed in Stomach (37.6).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for CA9 Gene

SOURCE GeneReport for Unigene cluster for CA9 Gene Hs.63287

mRNA Expression by UniProt/SwissProt for CA9 Gene

Tissue specificity: Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa
genes like me logo Genes that share expressions with CA9: view

In Situ Assay Products

Orthologs for CA9 Gene

This gene was present in the common ancestor of animals.

Orthologs for CA9 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia CA9 35
  • 99.34 (n)
  • 99.34 (a)
CA9 36
  • 99 (a)
(Bos Taurus)
Mammalia CA9 35
  • 84.94 (n)
  • 83.22 (a)
CA9 36
  • 82 (a)
(Canis familiaris)
Mammalia CA9 35
  • 85.98 (n)
  • 82.57 (a)
CA9 36
  • 82 (a)
(Mus musculus)
Mammalia Car9 35
  • 79.62 (n)
  • 73.79 (a)
Car9 16
Car9 36
  • 73 (a)
(Monodelphis domestica)
Mammalia CA9 36
  • 63 (a)
(Rattus norvegicus)
Mammalia Car9 35
  • 80.69 (n)
  • 75.4 (a)
(Gallus gallus)
Aves CA9 35
  • 55.05 (n)
  • 46.97 (a)
CA9 36
  • 43 (a)
(Anolis carolinensis)
Reptilia CA9 36
  • 57 (a)
(Danio rerio)
Actinopterygii ca9 35
  • 48.08 (n)
  • 40.12 (a)
ca9 36
  • 37 (a)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP013402 35
  • 48.4 (n)
  • 40.17 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG6074 37
  • 34 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.8628 36
  • 17 (a)
Species with no ortholog for CA9:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for CA9 Gene

Gene Tree for CA9 (if available)
Gene Tree for CA9 (if available)

Paralogs for CA9 Gene

Paralogs for CA9 Gene

Selected SIMAP similar genes for CA9 Gene using alignment to 1 proteins:

genes like me logo Genes that share paralogs with CA9: view

Variants for CA9 Gene

Sequence variations from dbSNP and Humsavar for CA9 Gene

SNP ID Clin Chr 09 pos Sequence Context AA Info Type MAF
rs1048638 -- 35,681,125(+) ACTGC(A/C)AAGAA utr-variant-3-prime, nc-transcript-variant
rs1243870 -- 35,680,534(+) CAAAC(A/G)GCCCT intron-variant, downstream-variant-500B
rs1538535 -- 35,681,302(+) TGTTC(C/T)TCCTT downstream-variant-500B
rs1890595 -- 35,679,594(+) GTATG(C/T)GCCTA intron-variant
rs2789751 -- 35,680,361(-) GGATT(C/G)TTTTG intron-variant, downstream-variant-500B

Structural Variations from Database of Genomic Variants (DGV) for CA9 Gene

Variant ID Type Subtype PubMed ID
dgv8168n71 CNV Gain 21882294
nsv466385 CNV Loss 19166990
nsv6528 CNV Insertion 18451855

Relevant External Links for CA9 Gene

HapMap Linkage Disequilibrium report

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CA9 Gene

Disorders for CA9 Gene

(3) University of Copenhagen DISEASES for CA9 Gene

(46) Novoseek inferred disease relationships for CA9 Gene

Disease -log(P) Hits PubMed IDs
renal clear cell carcinoma 83.3 24
carcinoma renal cell 78.4 34
metastatic renal cell carcinoma 70.8 5
tumors 66.9 254
renal cancer 63 9

Relevant External Links for CA9

Genetic Association Database (GAD)
Human Genome Epidemiology (HuGE) Navigator
genes like me logo Genes that share disorders with CA9: view

No data available for UniProtKB/Swiss-Prot for CA9 Gene

Publications for CA9 Gene

  1. Molecular cloning and immunogenicity of renal cell carcinoma- associated antigen G250. (PMID: 10709109) Grabmaier K. … Oosterwijk E. (Int. J. Cancer 2000) 3 4 23
  2. Cloning and characterization of MN, a human tumor-associated protein with a domain homologous to carbonic anhydrase and a putative helix- loop-helix DNA binding segment. (PMID: 8084592) Pastorek J. … Kettmann R. (Oncogene 1994) 3 4 23
  3. CA9 gene: single nucleotide polymorphism predicts metastatic renal cell carcinoma prognosis. (PMID: 19539328) de Martino M. … Belldegrun A.S. (J. Urol. 2009) 3 23 48
  4. TGF-beta upregulates tumor-associated carbonic anhydrase IX gene expression in Hep3B cells. (PMID: 19540352) Yildirim H. … KAPAskar F. (Cell Biol. Int. 2009) 3 23
  5. The role of carbonic anhydrase 9 in regulating extracellular and intracellular ph in three-dimensional tumor cell growths. (PMID: 19458084) Swietach P. … Vaughan-Jones R.D. (J. Biol. Chem. 2009) 3 23

Products for CA9 Gene

Sources for CA9 Gene

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