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Aliases for SRPK1 Gene

Aliases for SRPK1 Gene

  • SRSF Protein Kinase 1 2 3
  • SFRS Protein Kinase 1 2 3 4
  • Serine/Arginine-Rich Splicing Factor Kinase 1 2 3
  • SR-Protein-Specific Kinase 1 3 4
  • EC 2.7.11.1 4 64
  • Serine/Arginine-Rich Protein-Specific Kinase 1 4
  • Serine/Threonine-Protein Kinase SRPK1 3
  • SR Protein Kinase 1 2
  • SFRSK1 3

External Ids for SRPK1 Gene

Summaries for SRPK1 Gene

Entrez Gene Summary for SRPK1 Gene

  • This gene encodes a serine/arginine protein kinase specific for the SR (serine/arginine-rich domain) family of splicing factors. The protein localizes to the nucleus and the cytoplasm. It is thought to play a role in regulation of both constitutive and alternative splicing by regulating intracellular localization of splicing factors. Alternative splicing of this gene results in multiple transcript variants. Additional alternatively spliced transcript variants have been described for this gene, but their full length nature have not been determined.[provided by RefSeq, Jul 2010]

GeneCards Summary for SRPK1 Gene

SRPK1 (SRSF Protein Kinase 1) is a Protein Coding gene. Among its related pathways are Influenza A and mRNA Splicing - Major Pathway. GO annotations related to this gene include protein serine/threonine kinase activity and protein kinase activity. An important paralog of this gene is SRPK2.

UniProtKB/Swiss-Prot for SRPK1 Gene

  • Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Can influence additional steps of mRNA maturation, as well as other cellular activities, such as chromatin reorganization in somatic and sperm cells and cell cycle progression. Isoform 2 phosphorylates SFRS2, ZRSR2, LBR and PRM1. Isoform 2 phosphorylates SRSF1 using a directional (C-terminal to N-terminal) and a dual-track mechanism incorporating both processive phosphorylation (in which the kinase stays attached to the substrate after each round of phosphorylation) and distributive phosphorylation steps (in which the kinase and substrate dissociate after each phosphorylation event). The RS domain of SRSF1 binds first to a docking groove in the large lobe of the kinase domain of SRPK1. This induces certain structural changes in SRPK1 and/or RRM2 domain of SRSF1, allowing RRM2 to bind the kinase and initiate phosphorylation. The cycles continue for several phosphorylation steps in a processive manner (steps 1-8) until the last few phosphorylation steps (approximately steps 9-12). During that time, a mechanical stress induces the unfolding of the beta-4 motif in RRM2, which then docks at the docking groove of SRPK1. This also signals RRM2 to begin to dissociate, which facilitates SRSF1 dissociation after phosphorylation is completed. Isoform 2 can mediate hepatitis B virus (HBV) core protein phosphorylation. It plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles. Isoform 1 and isoform 2 can induce splicing of exon 10 in MAPT/TAU. The ratio of isoform 1/isoform 2 plays a decisive role in determining cell fate in K-562 leukaemic cell line: isoform 2 favors proliferation where as isoform 1 favors differentiation.

Gene Wiki entry for SRPK1 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for SRPK1 Gene

Genomics for SRPK1 Gene

Genomic Location for SRPK1 Gene

Start:
35,832,966 bp from pter
End:
35,921,342 bp from pter
Size:
88,377 bases
Orientation:
Minus strand

Genomic View for SRPK1 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for SRPK1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for SRPK1 Gene

Regulatory Elements for SRPK1 Gene

Proteins for SRPK1 Gene

  • Protein details for SRPK1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q96SB4-SRPK1_HUMAN
    Recommended name:
    SRSF protein kinase 1
    Protein Accession:
    Q96SB4
    Secondary Accessions:
    • B4DS61
    • Q12890
    • Q5R364
    • Q5R365
    • Q8IY12

    Protein attributes for SRPK1 Gene

    Size:
    655 amino acids
    Molecular mass:
    74325 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Monomer. Isoform 2 is found in a multisubunit complex containing seven proteins, named toposome, which separates entangled circular chromatin DNA during chromosome segregation. Isoform 2 interacts with HHV-1 ICP27 protein. Isoform 2 interacts with DNAJB1/HSP40 and AHSA1/AHA1 and this mediates formation of a complex with the Hsp70 /Hsp90 machinery. Isoform 1 is found in a complex with: DHX9, MOV10, MATR3, HNRNPU, NCL, DDX21, HSD17B4, PABPC1, HNRNPM, IGF2BP1, SYNCRIP, RPL3, VIM, YBX1, NPM1, HNRNPA2B1, HNRNPC, RPLP0, RPL7A and RALY. Isoform 2 binds to IGF2BP1, SYNCRIP, HNRNPA2B1 and HNRNPC. Isoform 1 and isoform 2 interact with SAFB which inhibits its activity. Isoform 2 interacts with SAFB2 which inhibits its activity.
    SequenceCaution:
    • Sequence=CAI20544.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=CAI20545.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for SRPK1 Gene

    Alternative splice isoforms for SRPK1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for SRPK1 Gene

Proteomics data for SRPK1 Gene at MOPED

Post-translational modifications for SRPK1 Gene

  • Modification sites at PhosphoSitePlus
  • Ubiquitination at Lys215, Lys494, and Lys506

Domains for SRPK1 Gene

Gene Families for SRPK1 Gene

Protein Domains for SRPK1 Gene

UniProtKB/Swiss-Prot:

SRPK1_HUMAN
Domain:
  • Contains 1 protein kinase domain.:
    • Q96SB4
Family:
  • Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.:
    • Q96SB4
genes like me logo Genes that share domains with SRPK1: view

Function for SRPK1 Gene

Molecular function for SRPK1 Gene

GENATLAS Biochemistry: splicing factor,RS domain protein kinase 1,highly specific for the serine/arginine repeats,modulating the affinity between RS domain containing proteins and regulating the cellular distribution of their substrates
UniProtKB/Swiss-Prot CatalyticActivity: ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation: Activated by phosphorylation on Ser-51 and Ser-555.
UniProtKB/Swiss-Prot Function: Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Can influence additional steps of mRNA maturation, as well as other cellular activities, such as chromatin reorganization in somatic and sperm cells and cell cycle progression. Isoform 2 phosphorylates SFRS2, ZRSR2, LBR and PRM1. Isoform 2 phosphorylates SRSF1 using a directional (C-terminal to N-terminal) and a dual-track mechanism incorporating both processive phosphorylation (in which the kinase stays attached to the substrate after each round of phosphorylation) and distributive phosphorylation steps (in which the kinase and substrate dissociate after each phosphorylation event). The RS domain of SRSF1 binds first to a docking groove in the large lobe of the kinase domain of SRPK1. This induces certain structural changes in SRPK1 and/or RRM2 domain of SRSF1, allowing RRM2 to bind the kinase and initiate phosphorylation. The cycles continue for several phosphorylation steps in a processive manner (steps 1-8) until the last few phosphorylation steps (approximately steps 9-12). During that time, a mechanical stress induces the unfolding of the beta-4 motif in RRM2, which then docks at the docking groove of SRPK1. This also signals RRM2 to begin to dissociate, which facilitates SRSF1 dissociation after phosphorylation is completed. Isoform 2 can mediate hepatitis B virus (HBV) core protein phosphorylation. It plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles. Isoform 1 and isoform 2 can induce splicing of exon 10 in MAPT/TAU. The ratio of isoform 1/isoform 2 plays a decisive role in determining cell fate in K-562 leukaemic cell line: isoform 2 favors proliferation where as isoform 1 favors differentiation.

Enzyme Numbers (IUBMB) for SRPK1 Gene

Gene Ontology (GO) - Molecular Function for SRPK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000287 magnesium ion binding IDA 11509566
GO:0004672 protein kinase activity TAS 8208298
GO:0004674 protein serine/threonine kinase activity IDA 11509566
GO:0004713 protein tyrosine kinase activity --
GO:0005515 protein binding IPI 10196197
genes like me logo Genes that share ontologies with SRPK1: view

Phenotypes for SRPK1 Gene

GenomeRNAi human phenotypes for SRPK1:
genes like me logo Genes that share phenotypes with SRPK1: view

No data available for Animal Models , Transcription Factor Targeting and HOMER Transcription for SRPK1 Gene

Localization for SRPK1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for SRPK1 Gene

Isoform 2: Cytoplasm. Nucleus. Nucleus matrix. Microsome. Note=Shuttles between the nucleus and the cytoplasm. Inhibition of the Hsp90 ATPase activity, osmotic stress and interaction with HHV-1 ICP27 protein can induce its translocation to the nucleus. KAT5/TIP60 inhibits its nuclear translocation.
Isoform 1: Cytoplasm. Nucleus matrix. Microsome. Note=Mainly localized in the microsomal fraction and the cytoplasm, and to a lesser extent in the nuclear matrix.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for SRPK1 Gene COMPARTMENTS Subcellular localization image for SRPK1 gene
Compartment Confidence
nucleus 5
cytosol 3
endoplasmic reticulum 3

Gene Ontology (GO) - Cellular Components for SRPK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA --
GO:0005654 nucleoplasm IDA --
GO:0005737 cytoplasm IDA --
GO:0005783 endoplasmic reticulum IEA --
GO:0005886 plasma membrane IDA --
genes like me logo Genes that share ontologies with SRPK1: view

Pathways for SRPK1 Gene

genes like me logo Genes that share pathways with SRPK1: view

Pathways by source for SRPK1 Gene

1 BioSystems pathway for SRPK1 Gene
1 KEGG pathway for SRPK1 Gene

Gene Ontology (GO) - Biological Process for SRPK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006397 mRNA processing IEA --
GO:0006468 protein phosphorylation IDA 11509566
GO:0007059 chromosome segregation IDA 15034300
GO:0008380 RNA splicing IEA --
GO:0016032 viral process IEA --
genes like me logo Genes that share ontologies with SRPK1: view

Compounds for SRPK1 Gene

(3) HMDB Compounds for SRPK1 Gene

Compound Synonyms Cas Number PubMed IDs
Adenosine triphosphate
  • 5'-(tetrahydrogen triphosphate) Adenosine
56-65-5
ADP
  • adenosindiphosphorsaeure
58-64-0
Magnesium
  • Magnesium
7439-95-4

(5) Novoseek inferred chemical compound relationships for SRPK1 Gene

Compound -log(P) Hits PubMed IDs
dsk-1 89.8 3
serine 69.3 12
arginine 55.3 1
cisplatin 28.9 11
gemcitabine 25 2
genes like me logo Genes that share compounds with SRPK1: view

Transcripts for SRPK1 Gene

Unigene Clusters for SRPK1 Gene

SRSF protein kinase 1:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for SRPK1 Gene

ExUns: 1 ^ 2a · 2b ^ 3a · 3b ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11a · 11b ^ 12 ^ 13 ^ 14 ^ 15 ^ 16
SP1: - -
SP2: - - - -
SP3:
SP4: -
SP5:
SP6:

Relevant External Links for SRPK1 Gene

GeneLoc Exon Structure for
SRPK1
ECgene alternative splicing isoforms for
SRPK1

Expression for SRPK1 Gene

mRNA expression in normal human tissues for SRPK1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for SRPK1 Gene

This gene is overexpressed in Whole Blood (7.9).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for SRPK1 Gene

SOURCE GeneReport for Unigene cluster for SRPK1 Gene Hs.443861

mRNA Expression by UniProt/SwissProt for SRPK1 Gene

Q96SB4-SRPK1_HUMAN
Tissue specificity: Isoform 2 is predominantly expressed in the testis but is also present at lower levels in heart, ovary, small intestine, liver, kidney, pancreas and skeletal muscle. Isoform 1 is only seen in the testis, at lower levels than isoform 2. Highly expressed in different erythroid and lymphoid cell lines, with isoform 2 being far more abundant than isoform 1.
genes like me logo Genes that share expressions with SRPK1: view

Orthologs for SRPK1 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for SRPK1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia SRPK1 36
  • 99.85 (n)
  • 99.69 (a)
SRPK1 37
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia SRPK1 36
  • 93.8 (n)
  • 95.85 (a)
SRPK1 37
  • 95 (a)
OneToOne
dog
(Canis familiaris)
Mammalia SRPK1 36
  • 95.29 (n)
  • 96.47 (a)
SRPK1 37
  • 69 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Srpk1 36
  • 89.2 (n)
  • 92.59 (a)
Srpk1 16
Srpk1 37
  • 92 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia mdo-mir-7265 37
  • 88 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia -- 37
  • 75 (a)
OneToMany
-- 37
  • 82 (a)
OneToMany
rat
(Rattus norvegicus)
Mammalia Srpk1 36
  • 87.53 (n)
  • 91.45 (a)
chicken
(Gallus gallus)
Aves SRPK1 36
  • 76.1 (n)
  • 81.23 (a)
SRPK1 37
  • 70 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia SRPK1 37
  • 78 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia Xl.24774 36
tropical clawed frog
(Silurana tropicalis)
Amphibia srpk1 36
  • 67.95 (n)
  • 74.96 (a)
Str.7492 36
zebrafish
(Danio rerio)
Actinopterygii srpk1a 36
  • 67.54 (n)
  • 72.64 (a)
srpk1a 37
  • 66 (a)
OneToMany
srpk1b 37
  • 64 (a)
OneToMany
wufc06h03 36
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP005322 36
  • 59.74 (n)
  • 63.55 (a)
fruit fly
(Drosophila melanogaster)
Insecta SRPK 36
  • 54.41 (n)
  • 52.29 (a)
SRPK 37
  • 40 (a)
OneToMany
SRPK 38
  • 73 (a)
BEST:CK01209 38
  • 57 (a)
CG8565 38
  • 43 (a)
worm
(Caenorhabditis elegans)
Secernentea spk-1 37
  • 29 (a)
OneToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes SKY1 37
  • 30 (a)
OneToMany
Alicante grape
(Vitis vinifera)
eudicotyledons Vvi.7716 36
thale cress
(Arabidopsis thaliana)
eudicotyledons SRPK4 36
  • 60.4 (n)
  • 59.7 (a)
rice
(Oryza sativa)
Liliopsida Os03g0748400 36
  • 56.96 (n)
  • 57.97 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 37
  • 49 (a)
OneToMany
Species with no ortholog for SRPK1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for SRPK1 Gene

ENSEMBL:
Gene Tree for SRPK1 (if available)
TreeFam:
Gene Tree for SRPK1 (if available)

Paralogs for SRPK1 Gene

Paralogs for SRPK1 Gene

Selected SIMAP similar genes for SRPK1 Gene using alignment to 9 proteins:

Pseudogenes.org Pseudogenes for SRPK1 Gene

genes like me logo Genes that share paralogs with SRPK1: view

Variants for SRPK1 Gene

Sequence variations from dbSNP and Humsavar for SRPK1 Gene

SNP ID Clin Chr 06 pos Sequence Context AA Info Type MAF
rs639421 -- 35,919,689(+) ACCAA(C/T)TTTTG intron-variant
rs644229 -- 35,918,423(+) TTGAA(C/T)CTGGT intron-variant
rs659532 -- 35,919,496(+) TAAAA(C/G)TATAA intron-variant
rs661658 -- 35,917,832(+) TTTCA(C/G/T)TATGA intron-variant
rs662713 -- 35,920,290(+) GCGTT(C/G)AGCAA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for SRPK1 Gene

Variant ID Type Subtype PubMed ID
dgv1940e1 CNV Complex 17122850
nsv821646 CNV Loss 15273396
dgv6834n71 CNV Gain 21882294
dgv1941e1 CNV Complex 17122850
nsv428143 CNV Gain 18775914
esv2731915 CNV Deletion 23290073
esv2676075 CNV Deletion 23128226

Relevant External Links for SRPK1 Gene

HapMap Linkage Disequilibrium report
SRPK1

Disorders for SRPK1 Gene

(2) Novoseek inferred disease relationships for SRPK1 Gene

Disease -log(P) Hits PubMed IDs
tumors 0 6
cancer 0 2

Relevant External Links for SRPK1

Genetic Association Database (GAD)
SRPK1
Human Genome Epidemiology (HuGE) Navigator
SRPK1
genes like me logo Genes that share disorders with SRPK1: view

No data available for UniProtKB/Swiss-Prot for SRPK1 Gene

Publications for SRPK1 Gene

  1. Localization of serine kinases, SRPK1 (SFRSK1) and SRPK2 (SFRSK2), specific for the SR family of splicing factors in mouse and human chromosomes. (PMID: 10198174) Wang H.Y. … Fu X.D. (Genomics 1999) 2 3 23
  2. SR protein-specific kinase 1 is highly expressed in testis and phosphorylates protamine 1. (PMID: 10390541) Papoutsopoulou S. … Giannakouros T. (Nucleic Acids Res. 1999) 3 4 23
  3. Cloning and characterization of an alternatively spliced form of SR protein kinase 1 that interacts specifically with scaffold attachment factor-B. (PMID: 11509566) Nikolakaki E. … Giannakouros T. (J. Biol. Chem. 2001) 3 4 23
  4. Interplay between SRPK and Clk/Sty kinases in phosphorylation of the splicing factor ASF/SF2 is regulated by a docking motif in ASF/SF2. (PMID: 16209947) Ngo J.C.K. … Ghosh G. (Mol. Cell 2005) 3 4 23
  5. Protein kinase CK2 phosphorylates and activates the SR protein- specific kinase 1. (PMID: 12565829) Mylonis I. … Giannakouros T. (Biochem. Biophys. Res. Commun. 2003) 3 4 23

Products for SRPK1 Gene

  • Addgene plasmids for SRPK1

Sources for SRPK1 Gene

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