MRPS35 Gene(Protein Coding)

Mitochondrial Ribosomal Protein S35

GCID:: GC12P027710

GIFtS:: 54

Genes Participants

Aliases for MRPS35 Gene

Mitochondrial Ribosomal Protein S35 ² ³ ⁵
28S Ribosomal Protein S28, Mitochondrial ³ ⁴
MRP-S28 ³ ⁴
MRP-S35 ³ ⁴
MRPS28 ³ ⁴
S28mt ³ ⁴

S35mt ³ ⁴
Mitochondrial Ribosomal Protein S28 ³
HDCMD11P ³
MDS023 ³

External Ids for MRPS35 Gene

HGNC: 16635
Entrez Gene: 60488
Ensembl: ENSG00000061794
OMIM: 611995
UniProtKB: P82673

Previous GeneCards Identifiers for MRPS35 Gene

GC12P028236
GC12P027887
GC12P027763
GC12P027754
GC12P027863
GC12P027632

Export aliases for MRPS35 gene to outside databases

Summaries for MRPS35 Gene

Entrez Gene Summary for MRPS35 Gene

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has had confusing nomenclature in the literature. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 3p, 5q, and 10q. [provided by RefSeq, Jul 2010]

GeneCards Summary for MRPS35 Gene

MRPS35 (Mitochondrial Ribosomal Protein S35) is a Protein Coding gene. Among its related pathways are Mitochondrial translation and Organelle biogenesis and maintenance. GO annotations related to this gene include poly(A) RNA binding and structural constituent of ribosome.

Gene Wiki entry for MRPS35 Gene

No data available for UniProtKB/Swiss-Prot , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MRPS35 Gene

Genomics for MRPS35 Gene

Regulatory Elements for MRPS35 Gene

Enhancers for MRPS35 Gene

GeneHancer Identifier	Score	Enhancer Sources	TSS distance (kb)	Number of Genes Away	Size (kb)	Transcription Factor Binding Sites within enhancer	Other Gene Targets for Enhancer

Enhancers around MRPS35 on UCSC Golden Path with GeneCards custom track

Promoters for MRPS35 Gene

Ensembl Regulatory Elements (ENSRs)	TSS Distance (bp)	Size (bp)	Binding Sites for Transcription Factors within promoters

ENSRs around MRPS35 on UCSC Golden Path with GeneCards custom track

Transcription factor binding sites by QIAGEN in the MRPS35 gene promoter:

See All at QIAGEN

Regulatory Element Products

Genomic Location for MRPS35 Gene

Chromosome:: 12

Start:: 27,710,773 bp from pter
End:: 27,756,304 bp from pter

Size:: 45,532 bases
Orientation:: Plus strand

Genomic View for MRPS35 Gene

Genes around MRPS35 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:

12p11.22 by Ensembl
12p11 by Entrez Gene
12p11 by HGNC

MRPS35 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)

Genomic Location for MRPS35 Gene — Genomic Neighborhood • Exon Structure • Gene Density

RefSeq DNA sequence for MRPS35 Gene

Proteins for MRPS35 Gene

Protein details for MRPS35 Gene (UniProtKB/Swiss-Prot)
Protein Symbol:

P82673-RT35_HUMAN

Recommended name:

28S ribosomal protein S35, mitochondrial

Protein Accession:

P82673

Secondary Accessions:
- Q32LZ1
- Q6P4C6
- Q7L1M6
- Q8NBP4
- Q96AI0
- Q9H044
- Q9HC14
- Q9P1R5
Protein attributes for MRPS35 Gene
Size:

323 amino acids

Molecular mass:

36844 Da
Quaternary structure:
Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins.
SequenceCaution:
Sequence=AAG14958.1; Type=Frameshift; Positions=97; Evidence={ECO:0000305};
Three dimensional structures from OCA and Proteopedia for MRPS35 Gene
- 3J9M (3D)
Alternative splice isoforms for MRPS35 Gene
UniProtKB/Swiss-Prot:
P82673-1

P82673-2

neXtProt entry for MRPS35 Gene

Proteomics data for MRPS35 Gene at MOPED

Protein Expression for MRPS35 Gene

See protein expression from ProteomicsDB, MOPED, PaxDb, and MaxQB

Post-translational modifications for MRPS35 Gene

Modification sites at PhosphoSitePlus
- P82673
Modification sites at neXtProt
- NX_P82673

Other Protein References for MRPS35 Gene

ENSEMBL proteins:

REFSEQ proteins:

Antibody Products

Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies

Browse R&D Systems for Antibodies

OriGene Antibodies for MRPS35
- TA315702
Custom Antibody Services

Novus Biologicals Antibodies for MRPS35

Abcam antibodies for MRPS35 (P82673)

Browse Antibodies at Cloud-Clone Corp.

Invitrogen Antibodies for MRPS35

antibodies-online Antibodies for MRPS35: See all 28

GeneTex MRPS35 antibody for MRPS35

Santa Cruz Biotechnology (SCBT) Antibodies for MRPS35
- MRP-S35 (I-15)
- MRP-S35 (P-14)

Protein Products

Browse Purified and Recombinant Proteins at EMD Millipore

Browse R&D Systems for Human Recombinant Proteins

Browse Enzo Life Sciences for proteins

OriGene Purified Proteins for MRPS35
Search Origene for MassSpec and Protein Over-expression Lysates for MRPS35
Origene Custom Protein Services for MRPS35

GenScript Custom Purified and Recombinant Proteins Services for MRPS35

Novus Biologicals proteins and lysates for MRPS35

Browse ProSpec Recombinant Proteins

Browse Proteins at Cloud-Clone Corp.

Search eBioscience for MRPS35 related proteins

Search GeneTex for Proteins for MRPS35

Assay Products

Browse Kits and Assays available from EMD Millipore

GenScript Custom Assay Services for MRPS35

Browse Enzo Life Sciences for kits & assays

Browse Assay Kits at Cloud-Clone Corp.

Search eBioscience for MRPS35 related elisas

antibodies-online Kits for MRPS35: See all 8

No data available for DME Specific Peptides for MRPS35 Gene

Domains & Families for MRPS35 Gene

Gene Families for MRPS35 Gene

HGNC:

646 : Mitochondrial ribosomal proteins

Protein Domains for MRPS35 Gene

InterPro:

Ribosomal_S24/S35_mit

ProtoNet:

P82673

Suggested Antigen Peptide Sequences for MRPS35 Gene

GenScript: Design optimal peptide antigens:

28S ribosomal protein S28, mitochondrial (RT35_HUMAN)

Graphical View of Domain Structure for InterPro Entry

No data available for UniProtKB/Swiss-Prot for MRPS35 Gene

Function for MRPS35 Gene

Phenotypes for MRPS35 Gene

MGI mutant phenotypes for MRPS35:

inferred from 1 alleles

GenomeRNAi human phenotypes for MRPS35:

Animal Models for MRPS35 Gene

MGI Knock Outs for MRPS35:

Mrps35 ^{tm1a(EUCOMM)Wtsi}

Animal Model Products

Taconic Biosciences: Generate A Custom CRISPR Mouse Model For Your Study

ViGene Biosciences lentiviral particle packaged cDNA for MRPS35 gene
- LH850042
- LH876989
ViGene Biosciences ready-to-package AAV shRNAs for MRPS35 gene
- SH876989
- SH850042
Search ViGene Biosciences for MRPS35

CRISPR Products

OriGene CRISPR knockouts for MRPS35
- KN205632
- KN310389

Santa Cruz Biotechnology (SCBT) CRISPR for MRPS35

GenScript: Design CRISPR guide RNA sequences for MRPS35

miRNA for MRPS35 Gene

miRTarBase miRNAs that target MRPS35

No data available for Molecular function , Enzyme Numbers (IUBMB) , Gene Ontology (GO) - Molecular Function , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for MRPS35 Gene

Localization for MRPS35 Gene

Subcellular locations from UniProtKB/Swiss-Prot for MRPS35 Gene

Mitochondrion.

P82673-RT35_HUMAN

Subcellular locations from

COMPARTMENTS

Jensen Localization Image for MRPS35 Gene

Compartment	Confidence
mitochondrion	5
cytosol	2
extracellular	1
nucleus	1

No data available for Gene Ontology (GO) - Cellular Components for MRPS35 Gene

Pathways & Interactions for MRPS35 Gene

SuperPathways for MRPS35 Gene

More details on SuperPathways for MRPS35

	Superpath	Contained pathways
1	Mitochondrial translation	Mitochondrial translation .93 Mitochondrial translation elongation .93	Mitochondrial translation initiation .93 Mitochondrial translation termination .93
2	Organelle biogenesis and maintenance	Organelle biogenesis and maintenance .57

Pathways by source for MRPS35 Gene

5 Reactome pathways for MRPS35 Gene

Interacting Proteins for MRPS35 Gene

http://string-db.org/version_10/api/image/networkList?limit=0&targetmode=proteins&caller_identity=gene_cards&network_flavor=evidence&identifiers=9606.ENSP00000081029%0d%0a9606.ENSP00000272418%0d%0a9606.ENSP00000310785%0d%0a9606.ENSP00000352706%0d%0a9606.ENSP00000241600%0d%0a9606.ENSP00000253686%0d%0a — STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

Selected Interacting proteins: P82673-RT35_HUMAN ENSP00000081029 for MRPS35 Gene via MINT STRING I2D

Symbol	External ID(s)	Details
ICT1	Q14197 ¹⁹ ²¹ ENSP00000301585 ²⁰	MINT-8079030 STRING: ENSP00000301585 I2D: score=1
DAP3	P51398 ¹⁹ ENSP00000341692 ²⁰	MINT-8079030 STRING: ENSP00000341692
ECI1	P42126 ¹⁹ ENSP00000301729 ²⁰	MINT-8079030 STRING: ENSP00000301729
HADHA	P40939 ¹⁹ ENSP00000370023 ²⁰	MINT-8079030 STRING: ENSP00000370023
LRPPRC	P42704 ¹⁹ ENSP00000260665 ²⁰	MINT-8079030 STRING: ENSP00000260665

Gene Ontology (GO) - Biological Process for MRPS35 Gene

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006996	organelle organization	TAS	--
GO:0070125	mitochondrial translational elongation	TAS	--

No data available for SIGNOR curated interactions for MRPS35 Gene

Drugs & Compounds for MRPS35 Gene

No Compound Related Data Available

Drug Products

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for MRPS35

Browse compounds at ApexBio

Transcripts for MRPS35 Gene

mRNA/cDNA for MRPS35 Gene

(2) REFSEQ mRNAs :

(10) Additional mRNA sequences :

(301) Selected AceView cDNA sequences:

(5) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for MRPS35 Gene

Mitochondrial ribosomal protein S35:

Hs.714076

Representative Sequences:

BC028346

CRISPR Products

OriGene CRISPR knockouts for MRPS35
- KN205632
- KN310389

Santa Cruz Biotechnology (SCBT) CRISPR for MRPS35

GenScript: Design CRISPR guide RNA sequences for MRPS35

miRNA Products

Search ViGene Biosciences for MRPS35

Inhibitory RNA Products

Origene siRNA, shRNA, mouse_rna, rat_shrna, and RNAi products in human, mouse, rat for MRPS35
Browse OriGene Inhibitory RNA Products For MRPS35

ViGene Biosciences ready-to-package AAV shRNAs for MRPS35 gene
- SH876989
- SH850042

Search Santa Cruz Biotechnology (SCBT) for MRPS35 siRNA/shRNA

Clone Products

OriGene clones in human for MRPS35
OriGene ORF clones in human,mouse,rat for MRPS35
Custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling

GenScript: cDNA clones in your preferred vector
- NM_021821
- NM_001190864

Sino Biological Human cDNA Clone for MRPS35

Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat

Search Addgene for plasmids for MRPS35

Flow Cytometry Products

eBioscience FlowRNA Probe Sets

Alternative Splicing Database (ASD) splice patterns (SP) for MRPS35 Gene

ExUns:	1a	·	1b	·	1c	^	2	^	3	^	4	^	5	^	6	^	7	^	8a	·	8b
SP1:
SP2:															-
SP3:							-		-		-		-		-		-

Relevant External Links for MRPS35 Gene

GeneLoc Exon Structure for: MRPS35
ECgene alternative splicing isoforms for: MRPS35

Expression for MRPS35 Gene

mRNA expression in normal human tissues for MRPS35 Gene

mRNA expression by BioGPS for MRPS35 Gene

Protein differential expression in normal tissues from HIPED for MRPS35 Gene

This gene is overexpressed in Pancreas (10.4) and Adrenal (8.4).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for MRPS35 Gene

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for MRPS35 Gene

Integrated proteomics: cell line protein expression from ProteomicsDB, MOPED and MaxQB for MRPS35 Gene

SOURCE GeneReport for Unigene cluster for MRPS35 Gene Hs.714076

Protein tissue co-expression partners for MRPS35 Gene

- Elite partner

Primer Products

OriGene qPCR primer pairs for MRPS35
- HP214235
OriGene qPCR primer pairs and template standards for MRPS35
- HK210914

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for MRPS35 Gene

Orthologs for MRPS35 Gene

This gene was present in the common ancestor of animals.

Orthologs for MRPS35 Gene

Organism	Taxonomy	Gene	Similarity	Type	Details
cow (Bos Taurus)	Mammalia	MRPS35 ³⁵	86.85 (n) 84.16 (a)		513438 NM_001075619.2 NP_001069087.1
cow (Bos Taurus)	Mammalia	MRPS35 ³⁶	83 (a)	OneToOne	5:82486885-82530466
dog (Canis familiaris)	Mammalia	MRPS35 ³⁵	87.06 (n) 85.09 (a)		477660 XM_005637027.1 XP_005637084.1
dog (Canis familiaris)	Mammalia	MRPS35 ³⁶	84 (a)	OneToOne	27:19984316-20026576
mouse (Mus musculus)	Mammalia	Mrps35 ³⁵	76.59 (n) 76.8 (a)		232536 NM_145573.2 NP_663548.2
		Mrps35 ¹⁶			147042764
		Mrps35 ³⁶	76 (a)	OneToOne	6:147042764-147070903
chimpanzee (Pan troglodytes)	Mammalia	MRPS35 ³⁵	98.04 (n) 97.5 (a)		738082 XM_003313771.2 XP_003313819.2
chimpanzee (Pan troglodytes)	Mammalia	MRPS35 ³⁶	97 (a)	OneToOne	12:60746883-60791405
rat (Rattus norvegicus)	Mammalia	Mrps35 ³⁵	77.12 (n) 78.37 (a)		297727 NM_001106628.1 NP_001100098.1
oppossum (Monodelphis domestica)	Mammalia	MRPS35 ³⁶	60 (a)	OneToOne	8:25919492-26007796
platypus (Ornithorhynchus anatinus)	Mammalia	MRPS35 ³⁶	69 (a)	OneToOne	Contig3994:16632-44921
chicken (Gallus gallus)	Aves	MRPS35 ³⁵	69.05 (n) 67.86 (a)		419048 NM_001278091.1 NP_001265020.1
chicken (Gallus gallus)	Aves	MRPS35 ³⁶	65 (a)	OneToOne	1:72450875-72476524
lizard (Anolis carolinensis)	Reptilia	-- ³⁶	70 (a)	OneToMany	5:82934016-82939677
lizard (Anolis carolinensis)	Reptilia	-- ³⁶	60 (a)	OneToMany	GL343579.1:103246-114777
tropical clawed frog (Silurana tropicalis)	Amphibia	mrps35 ³⁵	63.21 (n) 65.93 (a)		548768 NM_001016014.2 NP_001016014.1
tropical clawed frog (Silurana tropicalis)	Amphibia	Str.11071 ³⁵			141993900
African clawed frog (Xenopus laevis)	Amphibia	Xl.6303 ³⁵			BX851679.1
zebrafish (Danio rerio)	Actinopterygii	Dr.10387 ³⁵			57091436
		mrps35 ³⁵	61.65 (n) 63.55 (a)		503880 NM_001031669.1 NP_001026839.1
		mrps35 ³⁶	58 (a)	OneToOne	25:2375792-2397730
rainbow trout (Oncorhynchus mykiss)	Actinopterygii	Omy.5353 ³⁵			BX305844.1
fruit fly (Drosophila melanogaster)	Insecta	mRpS28 ³⁷	47 (a)
		mRpS35 ³⁵	52.95 (n) 50.38 (a)		38345 NM_079169.3 NP_523893.1
		mRpS35 ³⁶	41 (a)	OneToOne	3L:2780899-2782105
African malaria mosquito (Anopheles gambiae)	Insecta	AgaP_AGAP006802 ³⁵	51.23 (n) 52.96 (a)		1270264 XM_308945.4 XP_308945.4
worm (Caenorhabditis elegans)	Secernentea	Y39B6A.39 ³⁷	39 (a)
		mrps-35 ³⁵	51.19 (n) 41.95 (a)		180240 NM_001269908.1 NP_001256837.1
		mrps-35 ³⁶	30 (a)	OneToOne	V:18981319-18986129
sea squirt (Ciona savignyi)	Ascidiacea	-- ³⁶	40 (a)	OneToOne	reftig_49:2271888-2275950

Species with no ortholog for MRPS35:

A. gosspyii yeast (Ashbya gossypii)
Actinobacteria (Mycobacterium tuberculosis)
Alicante grape (Vitis vinifera)
alpha proteobacteria (Wolbachia pipientis)
amoeba (Dictyostelium discoideum)
Archea (Pyrococcus horikoshii)
baker's yeast (Saccharomyces cerevisiae)
barley (Hordeum vulgare)
beta proteobacteria (Neisseria meningitidis)
bread mold (Neurospora crassa)
Chromalveolata (Phytophthora infestans)
common water flea (Daphnia pulex)
corn (Zea mays)
E. coli (Escherichia coli)
filamentous fungi (Aspergillus nidulans)
Firmicute bacteria (Streptococcus pneumoniae)
fission yeast (Schizosaccharomyces pombe)
green algae (Chlamydomonas reinhardtii)
honey bee (Apis mellifera)
K. lactis yeast (Kluyveromyces lactis)
loblloly pine (Pinus taeda)
malaria parasite (Plasmodium falciparum)
medicago trunc (Medicago Truncatula)
moss (Physcomitrella patens)
orangutan (Pongo pygmaeus)
pig (Sus scrofa)
rice (Oryza sativa)
rice blast fungus (Magnaporthe grisea)
schistosome parasite (Schistosoma mansoni)
sea anemone (Nematostella vectensis)
sea urchin (Strongylocentrotus purpuratus)
sorghum (Sorghum bicolor)
soybean (Glycine max)
stem rust fungus (Puccinia graminis)
sugarcane (Saccharum officinarum)
thale cress (Arabidopsis thaliana)
tomato (Lycopersicon esculentum)
toxoplasmosis (Toxoplasma gondii)
Trichoplax (Trichoplax adhaerens)
wheat (Triticum aestivum)

Evolution for MRPS35 Gene

ENSEMBL:: Gene Tree for MRPS35 (if available)
TreeFam:: Gene Tree for MRPS35 (if available)

Paralogs for MRPS35 Gene

Pseudogenes.org Pseudogenes for MRPS35 Gene

No data available for Paralogs for MRPS35 Gene

Variants for MRPS35 Gene

Sequence variations from dbSNP and Humsavar for MRPS35 Gene

SNP ID	Clin	Chr 12 pos	Sequence Context	Type
rs35475802	-	27,710,859(+)	CCGCG(A/C)TCCCA	reference, missense
rs7302334	--	27,716,841(+)	aaaaa(A/T)ttagc	intron-variant
rs7302395	--	27,746,724(+)	ACTCA(C/G)GTTCT	intron-variant
rs12822020	--	27,750,761(+)	GATAG(C/T)GCCAC	intron-variant
rs12827496	--	27,730,240(+)	ATCAC(A/G)TTTAG	intron-variant

Structural Variations from Database of Genomic Variants (DGV) for MRPS35 Gene

Variant ID	Type	Subtype	PubMed ID
esv2745664	CNV	Deletion	23290073
nsv469178	CNV	Gain	19166990
esv270584	CNV	Insertion	20981092
esv272743	CNV	Insertion	20981092
esv2674076	CNV	Deletion	23128226
esv2745671	CNV	Deletion	23290073

Variation tolerance for MRPS35 Gene

Residual Variation Intolerance Score: 58.5% of all genes are more intolerant (likely to be disease-causing)

Gene Damage Index Score: 4.95; 68.02% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for MRPS35 Gene

HapMap Linkage Disequilibrium report: MRPS35

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MRPS35 Gene

Disorders for MRPS35 Gene

Relevant External Links for MRPS35

Genetic Association Database (GAD): MRPS35
Human Genome Epidemiology (HuGE) Navigator: MRPS35
Atlas of Genetics and Cytogenetics in Oncology and Haematology:: MRPS35

No disorders were found for MRPS35 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for MRPS35 Gene

Publications for MRPS35 Gene

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression. (PMID: 20877624) Hendrickson S.L. … O'Brien S.J. (PLoS ONE 2010) ³ ⁴⁸ ⁶⁷
The small subunit of the mammalian mitochondrial ribosome: identification of the full complement of ribosomal proteins present. (PMID: 11279123) Koc E.C. … Spremulli L.L. (J. Biol. Chem. 2001) ² ³
BioID-based Identification of Skp Cullin F-box (SCF)I^-TrCP1/2 E3 Ligase Substrates. (PMID: 25900982) Coyaud E. … Raught B. (Mol. Cell Proteomics 2015) ³
Temporal proteomics of NGF-TrkA signaling identifies an inhibitory role for the E3 ligase Cbl-b in neuroblastoma cell differentiation. (PMID: 25921289) Emdal K.B. … Olsen J.V. (Sci Signal 2015) ³
The BioPlex Network: A Systematic Exploration of the Human Interactome. (PMID: 26186194) Huttlin E.L. … Gygi S.P. (Cell 2015) ³