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Aliases for RAD9A Gene

Aliases for RAD9A Gene

  • RAD9 Homolog A (S. Pombe) 2 3
  • DNA Repair Exonuclease Rad9 Homolog A 3 4
  • HRAD9 3 4
  • RAD9 3 6
  • Cell Cycle Checkpoint Control Protein RAD9A 3
  • RAD9 (S. Pombe) Homolog 2
  • EC 3.1.11.2 4

External Ids for RAD9A Gene

Previous Symbols for RAD9A Gene

  • RAD9

Summaries for RAD9A Gene

Entrez Gene Summary for RAD9A Gene

  • This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

GeneCards Summary for RAD9A Gene

RAD9A (RAD9 Homolog A (S. Pombe)) is a Protein Coding gene. Among its related pathways are Cell Cycle, Mitotic and Chks in Checkpoint Regulation. GO annotations related to this gene include protein kinase binding and SH3 domain binding. An important paralog of this gene is RAD9B.

UniProtKB/Swiss-Prot for RAD9A Gene

  • Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3->5 double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

Gene Wiki entry for RAD9A Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RAD9A Gene

Genomics for RAD9A Gene

Genomic Location for RAD9A Gene

Start:
67,317,871 bp from pter
End:
67,398,412 bp from pter
Size:
80,542 bases
Orientation:
Plus strand

Genomic View for RAD9A Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for RAD9A Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RAD9A Gene

Regulatory Elements for RAD9A Gene

Transcription factor binding sites by QIAGEN in the RAD9A gene promoter:

Proteins for RAD9A Gene

  • Protein details for RAD9A Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q99638-RAD9A_HUMAN
    Recommended name:
    Cell cycle checkpoint control protein RAD9A
    Protein Accession:
    Q99638
    Secondary Accessions:
    • B2RCZ8
    • Q6FI29
    • Q96C41

    Protein attributes for RAD9A Gene

    Size:
    391 amino acids
    Molecular mass:
    42547 Da
    Quaternary structure:
    • Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD9A interacts with BCL2L1, FEN1, PRKCD, RAD9B, HUS1, RAD1, ABL1, RPA1, ATAD5 and RPA2. Interacts with DNAJC7 and RHNO1.

    Three dimensional structures from OCA and Proteopedia for RAD9A Gene

neXtProt entry for RAD9A Gene

Proteomics data for RAD9A Gene at MOPED

Post-translational modifications for RAD9A Gene

  • Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for RAD9A Gene

No data available for DME Specific Peptides for RAD9A Gene

Domains for RAD9A Gene

Protein Domains for RAD9A Gene

InterPro:
ProtoNet:

UniProtKB/Swiss-Prot:

RAD9A_HUMAN
Family:
  • Belongs to the rad9 family.:
    • Q99638
genes like me logo Genes that share domains with RAD9A: view

No data available for Gene Families for RAD9A Gene

Function for RAD9A Gene

Molecular function for RAD9A Gene

UniProtKB/Swiss-Prot CatalyticActivity: Exonucleolytic cleavage in the 3- to 5-direction to yield nucleoside 5-phosphates
UniProtKB/Swiss-Prot Function: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3->5 double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

Enzyme Numbers (IUBMB) for RAD9A Gene

Gene Ontology (GO) - Molecular Function for RAD9A Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 10359610
GO:0008408 3-5 exonuclease activity IDA 10713044
GO:0008853 exodeoxyribonuclease III activity IEA --
GO:0017124 SH3 domain binding IPI 11971963
GO:0019899 enzyme binding IPI 15326225
genes like me logo Genes that share ontologies with RAD9A: view
genes like me logo Genes that share phenotypes with RAD9A: view

Animal Models for RAD9A Gene

MGI Knock Outs for RAD9A:

miRNA for RAD9A Gene

miRTarBase miRNAs that target RAD9A

No data available for Transcription Factor Targeting and HOMER Transcription for RAD9A Gene

Localization for RAD9A Gene

Subcellular locations from UniProtKB/Swiss-Prot for RAD9A Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for RAD9A Gene COMPARTMENTS Subcellular localization image for RAD9A gene
Compartment Confidence
nucleus 5
cytosol 2
mitochondrion 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for RAD9A Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA 12628935
GO:0005654 nucleoplasm TAS --
GO:0005737 cytoplasm IEA --
GO:0030896 checkpoint clamp complex IEA --
genes like me logo Genes that share ontologies with RAD9A: view

Pathways for RAD9A Gene

genes like me logo Genes that share pathways with RAD9A: view

Gene Ontology (GO) - Biological Process for RAD9A Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000075 cell cycle checkpoint --
GO:0000076 DNA replication checkpoint TAS 8943031
GO:0000077 DNA damage checkpoint IMP 21659603
GO:0000738 DNA catabolic process, exonucleolytic --
GO:0006260 DNA replication TAS --
genes like me logo Genes that share ontologies with RAD9A: view

Compounds for RAD9A Gene

(1) Novoseek inferred chemical compound relationships for RAD9A Gene

Compound -log(P) Hits PubMed IDs
hydroxyurea 59.9 7
genes like me logo Genes that share compounds with RAD9A: view

Transcripts for RAD9A Gene

Unigene Clusters for RAD9A Gene

RAD9 homolog A (S. pombe):
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for RAD9A Gene

ExUns: 1 ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7a · 7b ^ 8 ^ 9 ^ 10
SP1:
SP2: -
SP3:
SP4:

Relevant External Links for RAD9A Gene

GeneLoc Exon Structure for
RAD9A
ECgene alternative splicing isoforms for
RAD9A

Expression for RAD9A Gene

mRNA expression in normal human tissues for RAD9A Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for RAD9A Gene

SOURCE GeneReport for Unigene cluster for RAD9A Gene Hs.655354

genes like me logo Genes that share expressions with RAD9A: view

Orthologs for RAD9A Gene

This gene was present in the common ancestor of animals.

Orthologs for RAD9A Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia RAD9A 36
  • 90.86 (n)
  • 94.62 (a)
RAD9A 37
  • 81 (a)
OneToOne
dog
(Canis familiaris)
Mammalia RAD9A 36
  • 87.44 (n)
  • 86.15 (a)
RAD9A 37
  • 81 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Rad9a 36
  • 84.49 (n)
  • 82.26 (a)
Rad9a 16
Rad9a 37
  • 82 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia RAD9A 37
  • 74 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia RAD9A 37
  • 57 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Rad9a 36
  • 84.4 (n)
  • 83.12 (a)
chicken
(Gallus gallus)
Aves RAD9 37
  • 55 (a)
OneToOne
RAD9A 36
  • 66.3 (n)
  • 59.4 (a)
lizard
(Anolis carolinensis)
Reptilia RAD9A 37
  • 57 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia Rad9 36
tropical clawed frog
(Silurana tropicalis)
Amphibia rad9a 36
  • 58.06 (n)
  • 62.09 (a)
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.520 36
zebrafish
(Danio rerio)
Actinopterygii rad9a 37
  • 50 (a)
OneToOne
zgc56197 36
fruit fly
(Drosophila melanogaster)
Insecta Rad9 36
  • 47.62 (n)
  • 33.83 (a)
Rad9 37
  • 19 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea hpr-9 37
  • 14 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 37
  • 28 (a)
OneToMany
Species with no ortholog for RAD9A:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chimpanzee (Pan troglodytes)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for RAD9A Gene

ENSEMBL:
Gene Tree for RAD9A (if available)
TreeFam:
Gene Tree for RAD9A (if available)

Paralogs for RAD9A Gene

Paralogs for RAD9A Gene

Selected SIMAP similar genes for RAD9A Gene using alignment to 6 proteins:

genes like me logo Genes that share paralogs with RAD9A: view

Variants for RAD9A Gene

Sequence variations from dbSNP and Humsavar for RAD9A Gene

SNP ID Clin Chr 11 pos Sequence Context AA Info Type MAF
rs872109 -- 67,396,662(+) AGGCC(G/T)CCCCT intron-variant
rs872110 -- 67,397,320(-) TGGGG(A/G)CCGGG synonymous-codon, reference, nc-transcript-variant
rs1624816 -- 67,392,850(+) TGCCC(C/T)GGGGT upstream-variant-2KB, intron-variant
rs1790730 -- 67,394,194(+) TTCTC(C/T)GGCCT intron-variant
rs2066490 -- 67,393,462(+) GAGAT(A/G)TGATG utr-variant-5-prime, intron-variant

Structural Variations from Database of Genomic Variants (DGV) for RAD9A Gene

Variant ID Type Subtype PubMed ID
nsv897796 CNV Loss 21882294
nsv897797 CNV Loss 21882294
nsv468603 CNV Loss 19166990
nsv897801 CNV Loss 21882294
nsv897804 CNV Gain 21882294
dgv1193n71 CNV Loss 21882294
dgv1194n71 CNV Loss 21882294
esv1010387 CNV Loss 20482838
nsv516453 CNV Loss 19592680

Relevant External Links for RAD9A Gene

HapMap Linkage Disequilibrium report
RAD9A
Human Gene Mutation Database (HGMD)
RAD9A

Disorders for RAD9A Gene

(5) Novoseek inferred disease relationships for RAD9A Gene

Disease -log(P) Hits PubMed IDs
lung carcinoma 20.3 2
nsclc 16.6 5
tumors 0 13
prostate cancer 0 3
cancer 0 6

Relevant External Links for RAD9A

Genetic Association Database (GAD)
RAD9A
Human Genome Epidemiology (HuGE) Navigator
RAD9A
genes like me logo Genes that share disorders with RAD9A: view

No data available for UniProtKB/Swiss-Prot for RAD9A Gene

Publications for RAD9A Gene

  1. The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1. (PMID: 10359610) St Onge R.P. … Davey S. (Mol. Biol. Cell 1999) 3 4 23
  2. Human DNA damage checkpoint protein hRAD9 is a 3' to 5' exonuclease. (PMID: 10713044) Bessho T. … Sancar A. (J. Biol. Chem. 2000) 3 4 23
  3. HDAC1, a histone deacetylase, forms a complex with Hus1 and Rad9, two G2/M checkpoint Rad proteins. (PMID: 10846170) Cai R.L. … Cohen D. (J. Biol. Chem. 2000) 3 4 23
  4. The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9. (PMID: 10884395) Rauen M. … Karnitz L.M. (J. Biol. Chem. 2000) 3 4 23
  5. The J domain of Tpr2 regulates its interaction with the proapoptotic and cell-cycle checkpoint protein, Rad9. (PMID: 11573955) Xiang S.L. … Yamamoto K.C. (Biochem. Biophys. Res. Commun. 2001) 3 4 23

Products for RAD9A Gene

Sources for RAD9A Gene

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