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Aliases for RAD1 Gene

Aliases for RAD1 Gene

  • RAD1 Checkpoint DNA Exonuclease 2 3
  • Rad1-Like DNA Damage Checkpoint Protein 3 4
  • Cell Cycle Checkpoint Protein Hrad1 2 3
  • DNA Repair Exonuclease Rad1 Homolog 3 4
  • Checkpoint Control Protein HRAD1 2 3
  • DNA Repair Exonuclease REC1 2 3
  • Exonuclease Homolog RAD1 2 3
  • HRAD1 3 4
  • REC1 3 4
  • Cell Cycle Checkpoint Protein RAD1 3
  • Rad1-Like DNA Damage Checkpoint 2
  • RAD1 (S. Pombe) Homolog 2
  • RAD1 Homolog (S. Pombe) 2
  • RAD1 Homolog 3
  • EC 3.1.11.2 4

External Ids for RAD1 Gene

Previous GeneCards Identifiers for RAD1 Gene

  • GC05M034941
  • GC05M035934
  • GC05M034953
  • GC05M034944
  • GC05M034854

Summaries for RAD1 Gene

Entrez Gene Summary for RAD1 Gene

  • This gene encodes a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. The 9-1-1 complex is recruited by RAD17 to affected sites where it may attract specialized DNA polymerases and other DNA repair effectors. Alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Jan 2009]

GeneCards Summary for RAD1 Gene

RAD1 (RAD1 Checkpoint DNA Exonuclease) is a Protein Coding gene. Among its related pathways are Cell Cycle, Mitotic and Chks in Checkpoint Regulation. GO annotations related to this gene include damaged DNA binding and exodeoxyribonuclease III activity.

UniProtKB/Swiss-Prot for RAD1 Gene

  • Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3->5 double stranded DNA exonuclease activity.

Gene Wiki entry for RAD1 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RAD1 Gene

Genomics for RAD1 Gene

Regulatory Elements for RAD1 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for RAD1 Gene

Start:
34,905,260 bp from pter
End:
34,918,989 bp from pter
Size:
13,730 bases
Orientation:
Minus strand

Genomic View for RAD1 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for RAD1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RAD1 Gene

Proteins for RAD1 Gene

  • Protein details for RAD1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O60671-RAD1_HUMAN
    Recommended name:
    Cell cycle checkpoint protein RAD1
    Protein Accession:
    O60671
    Secondary Accessions:
    • O75572
    • O95304
    • Q1W161
    • Q5KSM0
    • Q5KSM1
    • Q9UEP1

    Protein attributes for RAD1 Gene

    Size:
    282 amino acids
    Molecular mass:
    31827 Da
    Quaternary structure:
    • Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD1 interacts with POLB, FEN1, HUS1, HUS1B, RAD9A and RAD9B. Interacts with DNAJC7.
    SequenceCaution:
    • Sequence=AAC35550.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=AAC35550.1; Type=Frameshift; Positions=103; Evidence={ECO:0000305}; Sequence=CAA06249.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=CAA06249.1; Type=Frameshift; Positions=103; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for RAD1 Gene

    Alternative splice isoforms for RAD1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for RAD1 Gene

Proteomics data for RAD1 Gene at MOPED

Post-translational modifications for RAD1 Gene

  • Ubiquitination at Lys46
  • Modification sites at PhosphoSitePlus

Other Protein References for RAD1 Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

Antibody Products

No data available for DME Specific Peptides for RAD1 Gene

Domains for RAD1 Gene

Suggested Antigen Peptide Sequences for RAD1 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

O60671

UniProtKB/Swiss-Prot:

RAD1_HUMAN :
  • O60671
Family:
  • Belongs to the rad1 family.
genes like me logo Genes that share domains with RAD1: view

No data available for Gene Families for RAD1 Gene

Function for RAD1 Gene

Molecular function for RAD1 Gene

GENATLAS Biochemistry:
cell cycle G2 checkpoint control gene,also involved in DNA repair,homologous to RAD17,S pombe Rad1,S.cerevisiae,with two alternatively spliced isoforms (large RAD1-b and short RAD1-c),ubiquitously expressed,may be involved in lung carcinoma
UniProtKB/Swiss-Prot CatalyticActivity:
Exonucleolytic cleavage in the 3- to 5-direction to yield nucleoside 5-phosphates
UniProtKB/Swiss-Prot Function:
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3->5 double stranded DNA exonuclease activity.

Enzyme Numbers (IUBMB) for RAD1 Gene

Gene Ontology (GO) - Molecular Function for RAD1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003684 damaged DNA binding TAS 9872989
GO:0005515 protein binding IPI 10359610
GO:0008408 3-5 exonuclease activity IDA 9660799
GO:0008853 exodeoxyribonuclease III activity IEA --
genes like me logo Genes that share ontologies with RAD1: view

Phenotypes for RAD1 Gene

MGI mutant phenotypes for RAD1:
inferred from 1 alleles
GenomeRNAi human phenotypes for RAD1:
genes like me logo Genes that share phenotypes with RAD1: view

Animal Model Products

miRNA for RAD1 Gene

No data available for Animal Models , Transcription Factor Targeting and HOMER Transcription for RAD1 Gene

Localization for RAD1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RAD1 Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for RAD1 Gene COMPARTMENTS Subcellular localization image for RAD1 gene
Compartment Confidence
nucleus 5
cytoskeleton 2
cytosol 1
extracellular 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for RAD1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IC 9660799
GO:0005654 nucleoplasm TAS --
GO:0005694 chromosome IDA 9716408
GO:0030896 checkpoint clamp complex IBA --
GO:0043231 intracellular membrane-bounded organelle IDA --
genes like me logo Genes that share ontologies with RAD1: view

Pathways for RAD1 Gene

genes like me logo Genes that share pathways with RAD1: view

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

Gene Ontology (GO) - Biological Process for RAD1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000077 DNA damage checkpoint IMP 21659603
GO:0000738 DNA catabolic process, exonucleolytic --
GO:0006260 DNA replication TAS --
GO:0006281 DNA repair IEA --
GO:0006974 cellular response to DNA damage stimulus TAS 9872989
genes like me logo Genes that share ontologies with RAD1: view

Transcripts for RAD1 Gene

Unigene Clusters for RAD1 Gene

RAD1 homolog (S. pombe):
Representative Sequences:

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for RAD1

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for RAD1 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b ^ 7 ^ 8a · 8b · 8c
SP1: - -
SP2:
SP3: - - - - -
SP4: - - -
SP5:

Relevant External Links for RAD1 Gene

GeneLoc Exon Structure for
RAD1
ECgene alternative splicing isoforms for
RAD1

Expression for RAD1 Gene

mRNA expression in normal human tissues for RAD1 Gene

Protein differential expression in normal tissues for RAD1 Gene

This gene is overexpressed in Blymphocyte (15.2), Lymph node (12.7), Salivary gland (8.1), Adrenal (7.9), and CD8 Tcells (7.1).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for RAD1 Gene

SOURCE GeneReport for Unigene cluster for RAD1 Gene Hs.38114

mRNA Expression by UniProt/SwissProt for RAD1 Gene

O60671-RAD1_HUMAN
Tissue specificity: Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level).
genes like me logo Genes that share expressions with RAD1: view

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and Expression partners for RAD1 Gene

Orthologs for RAD1 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for RAD1 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia RAD1 35
  • 92.47 (n)
  • 96.42 (a)
RAD1 36
  • 96 (a)
OneToOne
dog
(Canis familiaris)
Mammalia RAD1 35
  • 92.17 (n)
  • 95.73 (a)
RAD1 36
  • 95 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Rad1 35
  • 87.26 (n)
  • 90.71 (a)
Rad1 16
Rad1 36
  • 91 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia RAD1 35
  • 99.88 (n)
  • 100 (a)
RAD1 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Rad1 35
  • 87.26 (n)
  • 90.36 (a)
oppossum
(Monodelphis domestica)
Mammalia RAD1 36
  • 89 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia RAD1 36
  • 88 (a)
OneToOne
chicken
(Gallus gallus)
Aves RAD1 35
  • 75.24 (n)
  • 83.21 (a)
RAD1 36
  • 83 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia RAD1 36
  • 88 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia rad1 35
  • 73.69 (n)
  • 85 (a)
Str.15014 35
African clawed frog
(Xenopus laevis)
Amphibia Rad1 35
zebrafish
(Danio rerio)
Actinopterygii rad1 35
  • 68.82 (n)
  • 76.26 (a)
zgc77779 35
rad1 36
  • 76 (a)
OneToOne
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.2152 35
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP002255 35
  • 44.4 (n)
  • 33.21 (a)
fruit fly
(Drosophila melanogaster)
Insecta Rad1 35
  • 44.36 (n)
  • 31.47 (a)
Rad1 36
  • 28 (a)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea mrt-2 35
  • 45.43 (n)
  • 29.84 (a)
mrt-2 36
  • 28 (a)
OneToOne
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.4289 35
bread mold
(Neurospora crassa)
Ascomycetes NCU00942 35
  • 42.35 (n)
  • 33.33 (a)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes rad1 35
  • 44.96 (n)
  • 31.01 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.9754 36
  • 60 (a)
OneToOne
Species with no ortholog for RAD1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for RAD1 Gene

ENSEMBL:
Gene Tree for RAD1 (if available)
TreeFam:
Gene Tree for RAD1 (if available)

Paralogs for RAD1 Gene

Pseudogenes.org Pseudogenes for RAD1 Gene

genes like me logo Genes that share paralogs with RAD1: view

No data available for Paralogs for RAD1 Gene

Variants for RAD1 Gene

Sequence variations from dbSNP and Humsavar for RAD1 Gene

SNP ID Clin Chr 05 pos Sequence Context AA Info Type MAF
rs37436 -- 34,912,714(-) TTGCC(A/G)AATGA intron-variant
rs192236 -- 34,911,127(-) tgatc(A/G)cctac intron-variant
rs342471 -- 34,905,307(+) GACAA(G/T)CAATG utr-variant-3-prime, nc-transcript-variant
rs342472 -- 34,908,250(+) ctcac(C/T)acaac utr-variant-3-prime, nc-transcript-variant
rs396519 -- 34,910,195(-) TGGTG(A/G)TAGGG intron-variant

Relevant External Links for RAD1 Gene

HapMap Linkage Disequilibrium report
RAD1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Structural Variations from Database of Genomic Variants (DGV) for RAD1 Gene

Disorders for RAD1 Gene

Relevant External Links for RAD1

Genetic Association Database (GAD)
RAD1
Human Genome Epidemiology (HuGE) Navigator
RAD1

No disorders were found for RAD1 Gene.

No data available for MalaCards , OMIM , UniProtKB/Swiss-Prot , University of Copenhagen DISEASES , Novoseek inferred disease relationships and Genatlas for RAD1 Gene

Publications for RAD1 Gene

  1. Human and mouse homologs of Schizosaccharomyces pombe rad1(+) and Saccharomyces cerevisiae RAD17: linkage to checkpoint control and mammalian meiosis. (PMID: 9716408) Freire R. … Jackson S.P. (Genes Dev. 1998) 2 3 4 23
  2. A human and mouse homolog of the Schizosaccharomyces pombe rad1+ cell cycle checkpoint control gene. (PMID: 9828137) Bluyssen H.A.R. … de Klein A. (Genomics 1998) 2 3 4 23
  3. RAD1, a human structural homolog of the Schizosaccharomyces pombe RAD1 cell cycle checkpoint gene. (PMID: 9828139) Marathi U.K. … Legerski R.J. (Genomics 1998) 3 4 23
  4. A human homologue of the Schizosaccharomyces pombe rad1+ checkpoint gene encodes an exonuclease. (PMID: 9660799) Parker A.E. … Luyten W.H.M.L. (J. Biol. Chem. 1998) 3 4 23
  5. cDNA cloning and gene mapping of human homologs for Schizosaccharomyces pombe rad17, rad1, and hus1 and cloning of homologs from mouse, Caenorhabditis elegans, and Drosophila melanogaster. (PMID: 9878245) Dean F.B. … O'Donnell M. (Genomics 1998) 3 4 23

Products for RAD1 Gene

Sources for RAD1 Gene

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