Free for academic non-profit institutions. Other users need a Commercial license

Aliases for PRKCI Gene

Aliases for PRKCI Gene

  • Protein Kinase C Iota 2 3 5
  • Atypical Protein Kinase C-Lambda/Iota 3 4
  • PRKC-Lambda/Iota 3 4
  • APKC-Lambda/Iota 3 4
  • EC 2.7.11.13 4 61
  • NPKC-Iota 3 4
  • DXS1179E 3 4
  • Protein Kinase C Iota Type 3
  • Protein Kinase C, Iota 2
  • EC 2.7.11 61
  • PKCI 3

External Ids for PRKCI Gene

Previous HGNC Symbols for PRKCI Gene

  • DXS1179E

Previous GeneCards Identifiers for PRKCI Gene

  • GC0XM096083
  • GC03P170989
  • GC03P171342
  • GC03P171261
  • GC03P171422
  • GC03P169940
  • GC03P167310

Summaries for PRKCI Gene

Entrez Gene Summary for PRKCI Gene

  • This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq, Jul 2008]

GeneCards Summary for PRKCI Gene

PRKCI (Protein Kinase C Iota) is a Protein Coding gene. Diseases associated with PRKCI include Glioblastoma and Lung Squamous Cell Carcinoma. Among its related pathways are CCR5 Pathway in Macrophages and Development VEGF signaling via VEGFR2 - generic cascades. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is PRKCZ.

UniProtKB/Swiss-Prot for PRKCI Gene

  • Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABL oncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-induced apoptosis. In cultured neurons, prevents amyloid beta protein-induced apoptosis by interrupting cell death process at a very early step. In glioblastoma cells, may function downstream of phosphatidylinositol 3-kinase (PI(3)K) and PDPK1 in the promotion of cell survival by phosphorylating and inhibiting the pro-apoptotic factor BAD. Can form a protein complex in non-small cell lung cancer (NSCLC) cells with PARD6A and ECT2 and regulate ECT2 oncogenic activity by phosphorylation, which in turn promotes transformed growth and invasion. In response to nerve growth factor (NGF), acts downstream of SRC to phosphorylate and activate IRAK1, allowing the subsequent activation of NF-kappa-B and neuronal cell survival. Functions in the organization of the apical domain in epithelial cells by phosphorylating EZR. This step is crucial for activation and normal distribution of EZR at the early stages of intestinal epithelial cell differentiation. Forms a protein complex with LLGL1 and PARD6B independently of PARD3 to regulate epithelial cell polarity. Plays a role in microtubule dynamics in the early secretory pathway through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). In human coronary artery endothelial cells (HCAEC), is activated by saturated fatty acids and mediates lipid-induced apoptosis.

Tocris Summary for PRKCI Gene

  • Protein kinase C (PKC) refers to a family of serine/threonine protein kinases grouped by their activation mechanism. Conventional PKCs (cPKC alpha-, betaI- , betaII- and gamma-) are activated by phosphatidylserine in a calcium dependent manner and can bind diacylglycerol.

Gene Wiki entry for PRKCI Gene

No data available for CIViC summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PRKCI Gene

Genomics for PRKCI Gene

Regulatory Elements for PRKCI Gene

Enhancers for PRKCI Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH03G169657 1.9 FANTOM5 Ensembl ENCODE dbSUPER 30.5 -558.7 -558698 12.4 PKNOX1 FOXA2 ARNT ARID4B SIN3A FEZF1 DMAP1 ZNF2 ZBTB7B KLF13 PRKCI ENSG00000270096 SLC7A14 ACTRT3 MECOM ENSG00000242795
GH03G169938 1.5 Ensembl ENCODE dbSUPER 27.9 -281.7 -281699 4.2 PKNOX1 FOXA2 FEZF1 ZNF2 TCF12 GLIS2 GATA2 CBX5 ZNF263 JUNB PRKCI LOC100128164 SEC62 LRRC31 SAMD7 GPR160 LRRC34 LRRIQ4 GC03P169939 ENSG00000244193
GH03G170221 1.7 FANTOM5 Ensembl ENCODE 12.9 +0.9 942 3.4 HDGF PKNOX1 CREB3L1 ARNT SIN3A ZNF2 ZBTB7B YY1 SLC30A9 ZNF766 ENSG00000270096 PRKCI GPR160 SLC7A14 ENSG00000270135 MYNN PHC3 LRRIQ4 GC03P170245
GH03G169762 1.4 Ensembl ENCODE 12.7 -457.9 -457947 3.1 HDGF CREB3L1 ARNT AGO1 ARID4B SIN3A DMAP1 ZNF2 YY1 CBX5 SEC62-AS1 PRKCI RPL22P1 LRRC34 ENSG00000277925 PIR60567
GH03G169964 1.4 ENCODE dbSUPER 12.3 -255.4 -255418 3.9 PKNOX1 FOXA2 CREB3L1 ARNT ZFP64 WRNIP1 ARID4B SIN3A DMAP1 ZNF2 LOC100128164 PRKCI LRRC31 GPR160 ENSG00000270096 SEC62-AS1
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around PRKCI on UCSC Golden Path with GeneCards custom track

Promoters for PRKCI Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000161583 436 2399 CREB3L1 YY1 SLC30A9 ZNF143 ZNF263 SP3 TBX21 MEF2D ZNF610 GLIS1

Genomic Location for PRKCI Gene

Chromosome:
3
Start:
170,222,365 bp from pter
End:
170,305,982 bp from pter
Size:
83,618 bases
Orientation:
Plus strand

Genomic View for PRKCI Gene

Genes around PRKCI on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PRKCI Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PRKCI Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PRKCI Gene

Proteins for PRKCI Gene

  • Protein details for PRKCI Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P41743-KPCI_HUMAN
    Recommended name:
    Protein kinase C iota type
    Protein Accession:
    P41743
    Secondary Accessions:
    • D3DNQ4
    • Q8WW06

    Protein attributes for PRKCI Gene

    Size:
    596 amino acids
    Molecular mass:
    68262 Da
    Quaternary structure:
    • Forms a complex with SQSTM1 and MP2K5 (By similarity). Interacts directly with SQSTM1 (Probable). Interacts with IKBKB. Interacts with PARD6A, PARD6B and PARD6G. Part of a quaternary complex containing aPKC, PARD3, a PARD6 protein (PARD6A, PARD6B or PARD6G) and a GTPase protein (CDC42 or RAC1). Part of a complex with LLGL1 and PARD6B. Interacts with ADAP1/CENTA1. Interaction with SMG1, through the ZN-finger domain, activates the kinase activity. Interacts with CDK7. Forms a complex with RAB2A and GAPDH involved in recruitment onto the membrane of vesicular tubular clusters (VTCs). Interacts with ECT2 (Thr-359 phosphorylated form). Interacts with VAMP2 (PubMed:17313651). Interacts with WDFY2 (via WD repeats 1-3) (PubMed:16792529).
    SequenceCaution:
    • Sequence=AAA60171.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=AAB17011.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=AAH22016.3; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for PRKCI Gene

neXtProt entry for PRKCI Gene

Selected DME Specific Peptides for PRKCI Gene

P41743:
  • DEDIDWVQTEK
  • VNGGDLM
  • GIIYRDLK
  • HPFFRNVDWD
  • CINCKLLVHK
  • IHVFPCVPE
  • FLNKDPKERLGC
  • RLYELNKDSEL
  • EGDPCTVSSQ
  • YRRGARRW
  • YAMKVVKKELV
  • FQAKRFNR
  • TSTFCGTPNYIAPEILRG
  • RVIGRGSYAKVLLVRLKK
  • NTEDYLFQ
  • IDQSEFEGFEYINPLL
  • YRDLKLDN
  • KERLGCHPQTGFADI
  • VDWWALGVL
  • QIRIPRSLSV
  • GHIKLTD
  • LHSCFQT
  • MEQKQVVPPFKPNISGEFGLDNFD
  • EPVQLTPDD
  • YIAPEIL
  • YGFSVDWWALGVLMFEMMAGRSPFDI
  • HQVRVKAYYRGDIMITHFEPSIS
  • RDLKLDN
  • CGTPNYIAPE

Post-translational modifications for PRKCI Gene

  • Phosphorylation at Thr-412 in the activation loop is not mandatory for activation (By similarity). Upon neuronal growth factor (NGF) stimulation, phosphorylated by SRC at Tyr-265, Tyr-280 and Tyr-334. Phosphorylation at Tyr-265 facilitates binding to KPNB1/importin-beta regulating entry of PRKCI into the nucleus. Phosphorylation on Tyr-334 is important for NF-kappa-B stimulation.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for PRKCI Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for PRKCI (PKCI)
  • Cloud-Clone Corp. Antibodies for PRKCI

Domains & Families for PRKCI Gene

Gene Families for PRKCI Gene

Suggested Antigen Peptide Sequences for PRKCI Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

P41743

UniProtKB/Swiss-Prot:

KPCI_HUMAN :
  • The PB1 domain mediates interaction with SQSTM1.
  • Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Domain:
  • The PB1 domain mediates interaction with SQSTM1.
  • The C1 zinc finger does not bind diacylglycerol (DAG).
  • The pseudosubstrate motif resembles the sequence around sites phosphorylated on target proteins, except the presence of a non-phosphorylatable residue in place of Ser, it modulates activity by competing with substrates.
Family:
  • Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
genes like me logo Genes that share domains with PRKCI: view

Function for PRKCI Gene

Molecular function for PRKCI Gene

GENATLAS Biochemistry:
protein kinase C,iota polypeptide
UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=13.5 uM for ATP (for recombinant purified PRKCI) {ECO:0000269 PubMed:10906326}; Vmax=7.4 pmol/min/mg enzyme {ECO:0000269 PubMed:10906326};
UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation:
Atypical PKCs (PRKCI and PRKCZ) exhibit an elevated basal enzymatic activity (that may be due to the interaction with SMG1 or SQSTM1) and are not regulated by diacylglycerol, phosphatidylserine, phorbol esters or calcium ions. Two specific sites, Thr-412 (activation loop of the kinase domain) and Thr-564 (turn motif), need to be phosphorylated for its full activation (By similarity). Might also be a target for novel lipid activators that are elevated during nutrient-stimulated insulin secretion.
UniProtKB/Swiss-Prot Function:
Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABL oncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-induced apoptosis. In cultured neurons, prevents amyloid beta protein-induced apoptosis by interrupting cell death process at a very early step. In glioblastoma cells, may function downstream of phosphatidylinositol 3-kinase (PI(3)K) and PDPK1 in the promotion of cell survival by phosphorylating and inhibiting the pro-apoptotic factor BAD. Can form a protein complex in non-small cell lung cancer (NSCLC) cells with PARD6A and ECT2 and regulate ECT2 oncogenic activity by phosphorylation, which in turn promotes transformed growth and invasion. In response to nerve growth factor (NGF), acts downstream of SRC to phosphorylate and activate IRAK1, allowing the subsequent activation of NF-kappa-B and neuronal cell survival. Functions in the organization of the apical domain in epithelial cells by phosphorylating EZR. This step is crucial for activation and normal distribution of EZR at the early stages of intestinal epithelial cell differentiation. Forms a protein complex with LLGL1 and PARD6B independently of PARD3 to regulate epithelial cell polarity. Plays a role in microtubule dynamics in the early secretory pathway through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). In human coronary artery endothelial cells (HCAEC), is activated by saturated fatty acids and mediates lipid-induced apoptosis.

Enzyme Numbers (IUBMB) for PRKCI Gene

Gene Ontology (GO) - Molecular Function for PRKCI Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding IEA --
GO:0004672 protein kinase activity IDA 11724794
GO:0004674 protein serine/threonine kinase activity TAS --
GO:0004697 protein kinase C activity ISS --
GO:0005515 protein binding IPI 11257119
genes like me logo Genes that share ontologies with PRKCI: view
genes like me logo Genes that share phenotypes with PRKCI: view

Animal Models for PRKCI Gene

MGI Knock Outs for PRKCI:

Animal Model Products

  • Taconic Biosciences Mouse Models for PRKCI

CRISPR Products

miRNA for PRKCI Gene

Inhibitory RNA Products

Clone Products

  • Addgene plasmids for PRKCI

Flow Cytometry Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for PRKCI Gene

Localization for PRKCI Gene

Subcellular locations from UniProtKB/Swiss-Prot for PRKCI Gene

Cytoplasm. Membrane. Endosome. Nucleus. Note=Transported into the endosome through interaction with SQSTM1/p62. After phosphorylation by SRC, transported into the nucleus through interaction with KPNB1. Colocalizes with CDK7 in the cytoplasm and nucleus. Transported to vesicular tubular clusters (VTCs) through interaction with RAB2A. {ECO:0000269 PubMed:15695176, ECO:0000269 PubMed:9566925}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for PRKCI gene
Compartment Confidence
extracellular 5
cytoskeleton 5
nucleus 5
cytosol 5
plasma membrane 4
endosome 3
golgi apparatus 2
peroxisome 1

Gene Ontology (GO) - Cellular Components for PRKCI Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000139 Golgi membrane IEA --
GO:0005634 nucleus IEA,IDA 11260256
GO:0005737 cytoplasm IEA --
GO:0005768 endosome IEA --
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with PRKCI: view

Pathways & Interactions for PRKCI Gene

genes like me logo Genes that share pathways with PRKCI: view

Pathways by source for PRKCI Gene

SIGNOR curated interactions for PRKCI Gene

Activates:
Inactivates:
Is activated by:

Gene Ontology (GO) - Biological Process for PRKCI Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006468 protein phosphorylation IDA 8226978
GO:0006612 protein targeting to membrane NAS 11257119
GO:0007010 cytoskeleton organization NAS 11257119
GO:0007015 actin filament organization IEA --
GO:0010976 positive regulation of neuron projection development IMP 10467349
genes like me logo Genes that share ontologies with PRKCI: view

Drugs & Compounds for PRKCI Gene

(13) Drugs for PRKCI Gene - From: DrugBank, ApexBio, DGIdb, FDA Approved Drugs, HMDB, Tocris, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Tamoxifen Approved Pharma Antagonist, Target TGF-β modulatory and PKC inhibitory effects, ER antagonist, Anti-Estrogens 390
Ingenol Mebutate Approved Pharma 0
nolvadex Approved October 1998 Pharma 0
Adenosine triphosphate Approved Nutra 0
Rbt205 Inhibitor Experimental Pharma Target 0

(5) Additional Compounds for PRKCI Gene - From: HMDB