Free for academic non-profit institutions. Other users need a Commercial license

Aliases for PRKCH Gene

Aliases for PRKCH Gene

  • Protein Kinase C, Eta 2 3
  • PRKCL 3 4 6
  • PKCL 3 4 6
  • EC 2.7.11.13 4 64
  • NPKC-Eta 3 4
  • PKC-L 3 4
  • Protein Kinase C Eta Type 3
  • EC 2.7.11 64

External Ids for PRKCH Gene

Previous Symbols for PRKCH Gene

  • PRKCL

Summaries for PRKCH Gene

Entrez Gene Summary for PRKCH Gene

  • Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. [provided by RefSeq, Jul 2008]

GeneCards Summary for PRKCH Gene

PRKCH (Protein Kinase C, Eta) is a Protein Coding gene. Diseases associated with PRKCH include stroke, ischemic. Among its related pathways are Signaling by GPCR and Development FGFR signaling pathway. GO annotations related to this gene include enzyme binding and protein kinase C activity. An important paralog of this gene is PRKCB.

UniProtKB/Swiss-Prot for PRKCH Gene

  • Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. Associates with the cyclin CCNE1-CDK2-CDKN1B complex and inhibits CDK2 kinase activity, leading to RB1 dephosphorylation and thereby G1 arrest in keratinocytes. In association with RALA activates actin depolymerization, which is necessary for keratinocyte differentiation. In the pre-B cell receptor signaling, functions downstream of BLNK by up-regulating IRF4, which in turn activates L chain gene rearrangement. Regulates epithelial tight junctions (TJs) by phosphorylating occludin (OCLN) on threonine residues, which is necessary for the assembly and maintenance of TJs. In association with PLD2 and via TLR4 signaling, is involved in lipopolysaccharide (LPS)-induced RGS2 down-regulation and foam cell formation. Upon PMA stimulation, mediates glioblastoma cell proliferation by activating the mTOR pathway, the PI3K/AKT pathway and the ERK1-dependent phosphorylation of ELK1. Involved in the protection of glioblastoma cells from irradiation-induced apoptosis by preventing caspase-9 activation. In camptothecin-treated MCF-7 cells, regulates NF-kappa-B upstream signaling by activating IKBKB, and confers protection against DNA damage-induced apoptosis. Promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria. Phosphorylates ATF2 which promotes its nuclear retention and transcriptional activity and negatively regulates its mitochondrial localization.

Tocris Summary for PRKCH Gene

  • Protein kinase C (PKC) refers to a family of serine/threonine protein kinases grouped by their activation mechanism. Classical or conventional PKCs (cPKC alpha-, betaI- , betaII- and gamma-) are activated by phosphatidylserine in a calcium dependent manner and can bind diacylglycerol (DAG). The Ca2+ insensitive novel PKCs (nPKCs epsilon-, delta-, theta- and eta- isotypes) are also activated by DAG and phosphatidylserine. The atypical PKCs (aPKCs iota- and zeta-) are insensitive to Ca2+, DAG and phorbol esters. All PKCs isoforms consist of a highly conserved catalytic domain connected to a regulatory domain via a hinge region. The physiological roles of PKCs are vast. This due to PKC phosphorylation and activation of multiple target proteins, which are involved in signal transduction pathways including, but not limited to, receptor desensitization, modulation of membrane structure events, regulation of transcription, regulation of cell growth, immune responses, and in learning and memory.

Gene Wiki entry for PRKCH Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PRKCH Gene

Genomics for PRKCH Gene

Genomic Location for PRKCH Gene

Start:
61,187,559 bp from pter
End:
61,550,980 bp from pter
Size:
363,422 bases
Orientation:
Plus strand

Genomic View for PRKCH Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for PRKCH Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PRKCH Gene

Regulatory Elements for PRKCH Gene

Proteins for PRKCH Gene

  • Protein details for PRKCH Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P24723-KPCL_HUMAN
    Recommended name:
    Protein kinase C eta type
    Protein Accession:
    P24723
    Secondary Accessions:
    • B4DJN5
    • Q16246
    • Q8NE03

    Protein attributes for PRKCH Gene

    Size:
    683 amino acids
    Molecular mass:
    77828 Da
    Quaternary structure:
    • Interacts with FYN and RALA (By similarity). Interacts with DGKQ.

    Three dimensional structures from OCA and Proteopedia for PRKCH Gene

    Alternative splice isoforms for PRKCH Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for PRKCH Gene

Proteomics data for PRKCH Gene at MOPED

Post-translational modifications for PRKCH Gene

  • Modification sites at PhosphoSitePlus
  • Ubiquitination at Lys36 and Lys404

Domains for PRKCH Gene

UniProtKB/Swiss-Prot:

KPCL_HUMAN
Domain:
  • The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain:
    • P24723
  • Contains 1 AGC-kinase C-terminal domain.:
    • P24723
  • Contains 1 C2 domain.:
    • P24723
  • Contains 1 protein kinase domain.:
    • P24723
Family:
  • Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.:
    • P24723
Similarity:
  • Contains 2 phorbol-ester/DAG-type zinc fingers.:
    • P24723
genes like me logo Genes that share domains with PRKCH: view

Function for PRKCH Gene

Molecular function for PRKCH Gene

UniProtKB/Swiss-Prot CatalyticActivity: ATP + a protein = ADP + a phosphoprotein
UniProtKB/Swiss-Prot EnzymeRegulation: Novel PKCs (PRKCD, PRKCE, PRKCH and PRKCQ) are calcium-insensitive, but activated by diacylglycerol (DAG) and phosphatidylserine. Three specific sites; Thr-513 (activation loop of the kinase domain), Thr-656 (turn motif) and Ser-675 (hydrophobic region), need to be phosphorylated for its full activation
UniProtKB/Swiss-Prot Function: Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. Associates with the cyclin CCNE1-CDK2-CDKN1B complex and inhibits CDK2 kinase activity, leading to RB1 dephosphorylation and thereby G1 arrest in keratinocytes. In association with RALA activates actin depolymerization, which is necessary for keratinocyte differentiation. In the pre-B cell receptor signaling, functions downstream of BLNK by up-regulating IRF4, which in turn activates L chain gene rearrangement. Regulates epithelial tight junctions (TJs) by phosphorylating occludin (OCLN) on threonine residues, which is necessary for the assembly and maintenance of TJs. In association with PLD2 and via TLR4 signaling, is involved in lipopolysaccharide (LPS)-induced RGS2 down-regulation and foam cell formation. Upon PMA stimulation, mediates glioblastoma cell proliferation by activating the mTOR pathway, the PI3K/AKT pathway and the ERK1-dependent phosphorylation of ELK1. Involved in the protection of glioblastoma cells from irradiation-induced apoptosis by preventing caspase-9 activation. In camptothecin-treated MCF-7 cells, regulates NF-kappa-B upstream signaling by activating IKBKB, and confers protection against DNA damage-induced apoptosis. Promotes oncogenic functions of ATF2 in the nucleus while blocking its apoptotic function at mitochondria. Phosphorylates ATF2 which promotes its nuclear retention and transcriptional activity and negatively regulates its mitochondrial localization.

Enzyme Numbers (IUBMB) for PRKCH Gene

Gene Ontology (GO) - Molecular Function for PRKCH Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity --
GO:0004674 protein serine/threonine kinase activity --
GO:0004697 protein kinase C activity TAS 1986216
GO:0004699 calcium-independent protein kinase C activity IEA --
GO:0004713 protein tyrosine kinase activity --
genes like me logo Genes that share ontologies with PRKCH: view
genes like me logo Genes that share phenotypes with PRKCH: view

Animal Models for PRKCH Gene

MGI Knock Outs for PRKCH:

No data available for miRNA , Transcription Factor Targeting and HOMER Transcription for PRKCH Gene

Localization for PRKCH Gene

Subcellular locations from UniProtKB/Swiss-Prot for PRKCH Gene

Cytoplasm.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for PRKCH Gene COMPARTMENTS Subcellular localization image for PRKCH gene
Compartment Confidence
plasma membrane 5
cytosol 4
mitochondrion 2
golgi apparatus 1
nucleus 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for PRKCH Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IDA 15632189
GO:0005829 cytosol TAS --
GO:0005886 plasma membrane IDA 15632189
GO:0005911 cell-cell junction IEA --
GO:0016020 membrane --
genes like me logo Genes that share ontologies with PRKCH: view

Pathways for PRKCH Gene

genes like me logo Genes that share pathways with PRKCH: view

Gene Ontology (GO) - Biological Process for PRKCH Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006468 protein phosphorylation IMP 22304920
GO:0007165 signal transduction TAS 1986216
GO:0007596 blood coagulation TAS --
GO:0010744 positive regulation of macrophage derived foam cell differentiation ISS --
GO:0030168 platelet activation TAS --
genes like me logo Genes that share ontologies with PRKCH: view

Compounds for PRKCH Gene

(10) ApexBio Compounds for PRKCH Gene

Compound Action Cas Number
CX-4945 (Silmitasertib) potent and selective ATP-competitive small molecule protein kinase CK2 inhibitor [1009820-21-6]
Dequalinium Chloride PKC inhibitor [522-51-0]
Enzastaurin (LY317615) potent PKC beta inhibitor [170364-57-5]
GF 109203X Very potent and selective inhibitor of PKC [133052-90-1]
Go 6976 PKC inhibitor [136194-77-9]
Go 6983 Broad spectrum PKC inhibitor [133053-19-7]
K-252c PKC inhibitor [85753-43-1]
Ro 31-8220 cell-permeable inhibitor of PKC [125314-64-9]
Sotrastaurin (AEB071) Potent and specific inhibitor of PKC [425637-18-9]
Staurosporine (CGP 41251) inhibitor of a broad spectrum of protein kinases [62996-74-1]

(5) Tocris Compounds for PRKCH Gene

Compound Action Cas Number
GF 109203X Protein kinase C inhibitor [133052-90-1]
Go 6976 Potent protein kinase C inhibitor; selective for alpha and beta isozymes [136194-77-9]
Go 6983 Broad spectrum PKC inhibitor [133053-19-7]
Phorbol 12-myristate 13-acetate Protein kinase C activator [16561-29-8]
ZIP Cell-permeable inhibitor of atypical PKC isozyme PKMzeta [863987-12-6]

(846) HMDB Compounds for PRKCH Gene

Compound Synonyms Cas Number PubMed IDs
Adenosine triphosphate
  • 5'-(tetrahydrogen triphosphate) Adenosine
56-65-5
ADP
  • adenosindiphosphorsaeure
58-64-0
Calcium
  • Ca
7440-70-2
DG(14:0/14:0/0:0)
  • DAG(14:0/14:0)
Not Available
DG(14:0/14:1(9Z)/0:0)
  • DAG(14:0/14:1)
Not Available

(4) Novoseek inferred chemical compound relationships for PRKCH Gene

Compound -log(P) Hits PubMed IDs
cholesterol-sulfate 73.9 3
rapamycin 26.6 1
nitric oxide 12 5
cholesterol 0 1
genes like me logo Genes that share compounds with PRKCH: view

Transcripts for PRKCH Gene

Unigene Clusters for PRKCH Gene

Protein kinase C, eta:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for PRKCH Gene

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11a · 11b ^ 12 ^ 13a · 13b ^ 14 ^ 15 ^ 16 ^ 17 ^ 18a · 18b ^ 19a · 19b
SP1: - - - - - - -
SP2: - -
SP3:
SP4: -
SP5:
SP6: - - - -
SP7: - - -
SP8:

Relevant External Links for PRKCH Gene

GeneLoc Exon Structure for
PRKCH
ECgene alternative splicing isoforms for
PRKCH

Expression for PRKCH Gene

mRNA expression in normal human tissues for PRKCH Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for PRKCH Gene

This gene is overexpressed in Whole Blood (4.4).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for PRKCH Gene

SOURCE GeneReport for Unigene cluster for PRKCH Gene Hs.333907

mRNA Expression by UniProt/SwissProt for PRKCH Gene

P24723-KPCL_HUMAN
Tissue specificity: Most abundant in lung, less in heart and skin.
genes like me logo Genes that share expressions with PRKCH: view

Orthologs for PRKCH Gene

This gene was present in the common ancestor of animals.

Orthologs for PRKCH Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia PRKCH 36
  • 99.61 (n)
  • 99.85 (a)
PRKCH 37
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia PRKCH 36
  • 92.42 (n)
  • 98.09 (a)
PRKCH 37
  • 98 (a)
OneToOne
dog
(Canis familiaris)
Mammalia PRKCH 36
  • 93.07 (n)
  • 97.66 (a)
PRKCH 37
  • 98 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Prkch 36
  • 90.03 (n)
  • 97.8 (a)
Prkch 16
Prkch 37
  • 98 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia PRKCH 37
  • 94 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia PRKCH 37
  • 80 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Prkch 36
  • 90.09 (n)
  • 97.36 (a)
chicken
(Gallus gallus)
Aves PRKCH 36
  • 79.31 (n)
  • 86.45 (a)
PRKCH 37
  • 79 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia PRKCH 37
  • 86 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia prkch 36
  • 72.1 (n)
  • 77.48 (a)
zebrafish
(Danio rerio)
Actinopterygii prkcha 36
  • 66.22 (n)
  • 67.9 (a)
prkcha 37
  • 63 (a)
OneToMany
prkchb 37
  • 62 (a)
OneToMany
fruit fly
(Drosophila melanogaster)
Insecta Pkc98E 37
  • 51 (a)
OneToMany
Pkc98E 38
  • 53 (a)
Pkcdgr 38
  • 47 (a)
worm
(Caenorhabditis elegans)
Secernentea pkc-1 37
  • 51 (a)
OneToMany
R12G8.1 38
  • 46 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.2703 37
  • 48 (a)
OneToMany
Species with no ortholog for PRKCH:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for PRKCH Gene

ENSEMBL:
Gene Tree for PRKCH (if available)
TreeFam:
Gene Tree for PRKCH (if available)

Paralogs for PRKCH Gene

Paralogs for PRKCH Gene

Pseudogenes.org Pseudogenes for PRKCH Gene

genes like me logo Genes that share paralogs with PRKCH: view

Variants for PRKCH Gene

Sequence variations from dbSNP and Humsavar for PRKCH Gene

SNP ID Clin Chr 14 pos Sequence Context AA Info Type MAF
rs14095 -- 61,550,925(+) CGAAT(A/G)TACAG utr-variant-3-prime
rs718028 -- 61,357,508(-) ATAAA(C/T)GTGGC intron-variant
rs752180 -- 61,377,535(-) CCTTT(A/G)CAGAA intron-variant
rs764153 -- 61,326,807(+) GGACA(C/T)GGTCT intron-variant
rs767755 -- 61,407,730(+) ACTTA(A/G)GTACA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for PRKCH Gene

Variant ID Type Subtype PubMed ID
nsv902005 CNV Gain 21882294
esv1002305 CNV Loss 20482838
esv2657744 CNV Deletion 23128226
nsv514743 CNV Loss 21397061
nsv1297 CNV Insertion 18451855
nsv442341 CNV CNV 18776908
esv2422159 CNV Deletion 20811451
nsv517729 CNV Loss 19592680
nsv902006 CNV Loss 21882294
esv2663429 CNV Deletion 23128226
nsv470650 CNV Loss 18288195
nsv517477 CNV Loss 19592680

Relevant External Links for PRKCH Gene

HapMap Linkage Disequilibrium report
PRKCH
Human Gene Mutation Database (HGMD)
PRKCH

Disorders for PRKCH Gene

(1) OMIM Diseases for PRKCH Gene (605437)

UniProtKB/Swiss-Prot

KPCL_HUMAN
  • Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. {ECO:0000269 PubMed:17206144}. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.

(1) MalaCards Diseases for PRKCH Gene

Search for PRKCH Gene in MalaCards »

(3) Novoseek inferred disease relationships for PRKCH Gene

Disease -log(P) Hits PubMed IDs
glioblastoma 48.5 4
rheumatoid arthritis 3 1
tumors 0 1

Relevant External Links for PRKCH

Genetic Association Database (GAD)
PRKCH
Human Genome Epidemiology (HuGE) Navigator
PRKCH
genes like me logo Genes that share disorders with PRKCH: view

Publications for PRKCH Gene

  1. Protein kinase C-eta regulates resistance to UV- and gamma- irradiation-induced apoptosis in glioblastoma cells by preventing caspase-9 activation. (PMID: 11772428) Hussaini I.M. … Vandenberg S.R. (Neuro-oncol. 2002) 3 4 23
  2. PKC-eta mediates glioblastoma cell proliferation through the Akt and mTOR signaling pathways. (PMID: 15489897) Aeder S.E. … Hussaini I.M. (Oncogene 2004) 3 4 23
  3. The protein kinase C-eta isoform induces proliferation in glioblastoma cell lines through an ERK/Elk-1 pathway. (PMID: 17146445) Uht R.M. … Hussaini I.M. (Oncogene 2007) 3 4 23
  4. Genetic association between the PRKCH gene encoding protein kinase Ceta isozyme and rheumatoid arthritis in the Japanese population. (PMID: 17195206) Takata Y. … Itakura M. (Arthritis Rheum. 2007) 3 23 49
  5. A nonsynonymous SNP in PRKCH (protein kinase C eta) increases the risk of cerebral infarction. (PMID: 17206144) Kubo M. … Kiyohara Y. (Nat. Genet. 2007) 3 4 23

Products for PRKCH Gene

  • Addgene plasmids for PRKCH

Sources for PRKCH Gene

Back to Top

Content