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Aliases for PRKCE Gene

Aliases for PRKCE Gene

  • Protein Kinase C, Epsilon 2 3
  • NPKC-Epsilon 3 4
  • EC 2.7.11.13 4 63
  • PKCE 3 4
  • EC 2.7.11 63

External Ids for PRKCE Gene

Previous GeneCards Identifiers for PRKCE Gene

  • GC02P045897
  • GC02P046047
  • GC02P045853
  • GC02P045790

Summaries for PRKCE Gene

Entrez Gene Summary for PRKCE Gene

  • Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq, Jul 2008]

GeneCards Summary for PRKCE Gene

PRKCE (Protein Kinase C, Epsilon) is a Protein Coding gene. Among its related pathways are Immune System and DAG and IP3 signaling. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is PRKCB.

UniProtKB/Swiss-Prot for PRKCE Gene

  • Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays essential roles in the regulation of multiple cellular processes linked to cytoskeletal proteins, such as cell adhesion, motility, migration and cell cycle, functions in neuron growth and ion channel regulation, and is involved in immune response, cancer cell invasion and regulation of apoptosis. Mediates cell adhesion to the extracellular matrix via integrin-dependent signaling, by mediating angiotensin-2-induced activation of integrin beta-1 (ITGB1) in cardiac fibroblasts. Phosphorylates MARCKS, which phosphorylates and activates PTK2/FAK, leading to the spread of cardiomyocytes. Involved in the control of the directional transport of ITGB1 in mesenchymal cells by phosphorylating vimentin (VIM), an intermediate filament (IF) protein. In epithelial cells, associates with and phosphorylates keratin-8 (KRT8), which induces targeting of desmoplakin at desmosomes and regulates cell-cell contact. Phosphorylates IQGAP1, which binds to CDC42, mediating epithelial cell-cell detachment prior to migration. In HeLa cells, contributes to hepatocyte growth factor (HGF)-induced cell migration, and in human corneal epithelial cells, plays a critical role in wound healing after activation by HGF. During cytokinesis, forms a complex with YWHAB, which is crucial for daughter cell separation, and facilitates abscission by a mechanism which may implicate the regulation of RHOA. In cardiac myocytes, regulates myofilament function and excitation coupling at the Z-lines, where it is indirectly associated with F-actin via interaction with COPB1. During endothelin-induced cardiomyocyte hypertrophy, mediates activation of PTK2/FAK, which is critical for cardiomyocyte survival and regulation of sarcomere length. Plays a role in the pathogenesis of dilated cardiomyopathy via persistent phosphorylation of troponin I (TNNI3). Involved in nerve growth factor (NFG)-induced neurite outgrowth and neuron morphological change independently of its kinase activity, by inhibition of RHOA pathway, activation of CDC42 and cytoskeletal rearrangement. May be involved in presynaptic facilitation by mediating phorbol ester-induced synaptic potentiation. Phosphorylates gamma-aminobutyric acid receptor subunit gamma-2 (GABRG2), which reduces the response of GABA receptors to ethanol and benzodiazepines and may mediate acute tolerance to the intoxicating effects of ethanol. Upon PMA treatment, phosphorylates the capsaicin- and heat-activated cation channel TRPV1, which is required for bradykinin-induced sensitization of the heat response in nociceptive neurons. Is able to form a complex with PDLIM5 and N-type calcium channel, and may enhance channel activities and potentiates fast synaptic transmission by phosphorylating the pore-forming alpha subunit CACNA1B (CaV2.2). In prostate cancer cells, interacts with and phosphorylates STAT3, which increases DNA-binding and transcriptional activity of STAT3 and seems to be essential for prostate cancer cell invasion. Downstream of TLR4, plays an important role in the lipopolysaccharide (LPS)-induced immune response by phosphorylating and activating TICAM2/TRAM, which in turn activates the transcription factor IRF3 and subsequent cytokines production. In differentiating erythroid progenitors, is regulated by EPO and controls the protection against the TNFSF10/TRAIL-mediated apoptosis, via BCL2. May be involved in the regulation of the insulin-induced phosphorylation and activation of AKT1.

Tocris Summary for PRKCE Gene

  • Protein kinase C (PKC) refers to a family of serine/threonine protein kinases grouped by their activation mechanism. Conventional PKCs (cPKC alpha-, betaI- , betaII- and gamma-) are activated by phosphatidylserine in a calcium dependent manner and can bind diacylglycerol.

Gene Wiki entry for PRKCE Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PRKCE Gene

Genomics for PRKCE Gene

Regulatory Elements for PRKCE Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for PRKCE Gene

Chromosome:
2
Start:
45,651,315 bp from pter
End:
46,187,990 bp from pter
Size:
536,676 bases
Orientation:
Plus strand

Genomic View for PRKCE Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for PRKCE Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PRKCE Gene

Proteins for PRKCE Gene

  • Protein details for PRKCE Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q02156-KPCE_HUMAN
    Recommended name:
    Protein kinase C epsilon type
    Protein Accession:
    Q02156
    Secondary Accessions:
    • B0LPH7
    • Q32MQ3
    • Q53SL4
    • Q53SM5
    • Q9UE81

    Protein attributes for PRKCE Gene

    Size:
    737 amino acids
    Molecular mass:
    83674 Da
    Quaternary structure:
    • Forms a ternary complex with TRIM63 and GN2BL1. Can form a complex with PDLIM5 and N-type calcium channel. Interacts with COPB1 and YWHAB (By similarity). Interacts with DGKQ and STAT3.

    Three dimensional structures from OCA and Proteopedia for PRKCE Gene

neXtProt entry for PRKCE Gene

Proteomics data for PRKCE Gene at MOPED

Post-translational modifications for PRKCE Gene

  • Phosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729. Phosphorylation in the hinge domain at Ser-350 by MAPK11 or MAPK14, Ser-346 by GSK3B and Ser-368 by autophosphorylation is required for interaction with YWHAB.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for PRKCE Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for PRKCE (PKCE)

Domains & Families for PRKCE Gene

Suggested Antigen Peptide Sequences for PRKCE Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q02156

UniProtKB/Swiss-Prot:

KPCE_HUMAN :
  • The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain
  • Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
  • Contains 2 phorbol-ester/DAG-type zinc fingers.
Domain:
  • The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain
  • Contains 1 AGC-kinase C-terminal domain.
  • Contains 1 C2 domain.
  • Contains 1 protein kinase domain.
Family:
  • Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Similarity:
  • Contains 2 phorbol-ester/DAG-type zinc fingers.
genes like me logo Genes that share domains with PRKCE: view

Function for PRKCE Gene

Molecular function for PRKCE Gene

UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein
UniProtKB/Swiss-Prot EnzymeRegulation:
Novel PKCs (PRKCD, PRKCE, PRKCH and PRKCQ) are calcium-insensitive, but activated by diacylglycerol (DAG) and phosphatidylserine. Three specific sites; Thr-566 (activation loop of the kinase domain), Thr-710 (turn motif) and Ser-729 (hydrophobic region), need to be phosphorylated for its full activation
UniProtKB/Swiss-Prot Function:
Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays essential roles in the regulation of multiple cellular processes linked to cytoskeletal proteins, such as cell adhesion, motility, migration and cell cycle, functions in neuron growth and ion channel regulation, and is involved in immune response, cancer cell invasion and regulation of apoptosis. Mediates cell adhesion to the extracellular matrix via integrin-dependent signaling, by mediating angiotensin-2-induced activation of integrin beta-1 (ITGB1) in cardiac fibroblasts. Phosphorylates MARCKS, which phosphorylates and activates PTK2/FAK, leading to the spread of cardiomyocytes. Involved in the control of the directional transport of ITGB1 in mesenchymal cells by phosphorylating vimentin (VIM), an intermediate filament (IF) protein. In epithelial cells, associates with and phosphorylates keratin-8 (KRT8), which induces targeting of desmoplakin at desmosomes and regulates cell-cell contact. Phosphorylates IQGAP1, which binds to CDC42, mediating epithelial cell-cell detachment prior to migration. In HeLa cells, contributes to hepatocyte growth factor (HGF)-induced cell migration, and in human corneal epithelial cells, plays a critical role in wound healing after activation by HGF. During cytokinesis, forms a complex with YWHAB, which is crucial for daughter cell separation, and facilitates abscission by a mechanism which may implicate the regulation of RHOA. In cardiac myocytes, regulates myofilament function and excitation coupling at the Z-lines, where it is indirectly associated with F-actin via interaction with COPB1. During endothelin-induced cardiomyocyte hypertrophy, mediates activation of PTK2/FAK, which is critical for cardiomyocyte survival and regulation of sarcomere length. Plays a role in the pathogenesis of dilated cardiomyopathy via persistent phosphorylation of troponin I (TNNI3). Involved in nerve growth factor (NFG)-induced neurite outgrowth and neuron morphological change independently of its kinase activity, by inhibition of RHOA pathway, activation of CDC42 and cytoskeletal rearrangement. May be involved in presynaptic facilitation by mediating phorbol ester-induced synaptic potentiation. Phosphorylates gamma-aminobutyric acid receptor subunit gamma-2 (GABRG2), which reduces the response of GABA receptors to ethanol and benzodiazepines and may mediate acute tolerance to the intoxicating effects of ethanol. Upon PMA treatment, phosphorylates the capsaicin- and heat-activated cation channel TRPV1, which is required for bradykinin-induced sensitization of the heat response in nociceptive neurons. Is able to form a complex with PDLIM5 and N-type calcium channel, and may enhance channel activities and potentiates fast synaptic transmission by phosphorylating the pore-forming alpha subunit CACNA1B (CaV2.2). In prostate cancer cells, interacts with and phosphorylates STAT3, which increases DNA-binding and transcriptional activity of STAT3 and seems to be essential for prostate cancer cell invasion. Downstream of TLR4, plays an important role in the lipopolysaccharide (LPS)-induced immune response by phosphorylating and activating TICAM2/TRAM, which in turn activates the transcription factor IRF3 and subsequent cytokines production. In differentiating erythroid progenitors, is regulated by EPO and controls the protection against the TNFSF10/TRAIL-mediated apoptosis, via BCL2. May be involved in the regulation of the insulin-induced phosphorylation and activation of AKT1.

Enzyme Numbers (IUBMB) for PRKCE Gene

Gene Ontology (GO) - Molecular Function for PRKCE Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003785 actin monomer binding ISS --
GO:0004672 protein kinase activity --
GO:0004674 protein serine/threonine kinase activity IDA 15695813
GO:0004697 protein kinase C activity IDA 18556656
GO:0004699 calcium-independent protein kinase C activity IEA --
genes like me logo Genes that share ontologies with PRKCE: view
genes like me logo Genes that share phenotypes with PRKCE: view

Animal Models for PRKCE Gene

MGI Knock Outs for PRKCE:

Animal Model Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for PRKCE

In Situ Assay Products

Flow Cytometry Products

No data available for Transcription Factor Targets and HOMER Transcription for PRKCE Gene

Localization for PRKCE Gene

Subcellular locations from UniProtKB/Swiss-Prot for PRKCE Gene

Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cytoplasm, perinuclear region. Nucleus. Note=Translocated to plasma membrane in epithelial cells stimulated by HGF. Associated with the Golgi at the perinuclear site in pre-passage fibroblasts (By similarity). In passaging cells, translocated to the cell periphery (By similarity). Translocated to the nucleus in PMA-treated cells (By similarity). {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for PRKCE Gene COMPARTMENTS Subcellular localization image for PRKCE gene
Compartment Confidence
cytosol 5
plasma membrane 5
endoplasmic reticulum 4
cytoskeleton 3
nucleus 3
golgi apparatus 2
mitochondrion 2

Gene Ontology (GO) - Cellular Components for PRKCE Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005737 cytoplasm IDA 15632189
GO:0005739 mitochondrion IEA --
GO:0005783 endoplasmic reticulum IDA 18556656
GO:0005794 colocalizes_with Golgi apparatus ISS --
genes like me logo Genes that share ontologies with PRKCE: view

Pathways & Interactions for PRKCE Gene

SuperPathways for PRKCE Gene

Superpath Contained pathways
1 Interleukin receptor SHC signaling
2 IL-2 Pathway
3 DAG and IP3 signaling
4 Development Angiotensin activation of ERK
5 fMLP Pathway
genes like me logo Genes that share pathways with PRKCE: view

Pathways by source for PRKCE Gene

PCR Array Products

SIGNOR curated interactions for PRKCE Gene

Activates:
Inactivates:
Is activated by:
Is inactivated by:

Gene Ontology (GO) - Biological Process for PRKCE Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0002281 macrophage activation involved in immune response IEA --
GO:0006468 protein phosphorylation IDA 18556656
GO:0006915 apoptotic process TAS 10438519
GO:0007049 cell cycle IEA --
GO:0007155 cell adhesion IEA --
genes like me logo Genes that share ontologies with PRKCE: view

Drugs & Compounds for PRKCE Gene

(37) Drugs for PRKCE Gene - From: NovoSeek, HMDB, DGIdb, FDA Approved Drugs, ApexBio, Tocris, DrugBank, and PharmGKB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Tamoxifen Approved Pharma Antagonist, Target ER antagonist, Anti-Estrogens 341
Ingenol Mebutate Approved Pharma 0
nolvadex Approved October 1998 Pharma 0
Adenosine triphosphate Approved Nutra 0
Ethanol Approved Pharma 1708

(14) Additional Compounds for PRKCE Gene - From: HMDB, NovoSeek, and Tocris

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Antagonist, Gating inhibitor 58-64-0
Diglycerides Group A
  • 1,2-Dimyristoyl-rac-glycerol
  • DAG(14:0/14:0)
  • DAG(28:0)
  • DG(14:0/14:0)
  • DG(28:0)
Diglycerides Group B
  • 1-Palmitoleoyl-2-arachidonyl-sn-glycerol
  • DAG(16:1/20:0)
  • DAG(16:1n7/20:0)
  • DAG(16:1w7/20:0)
  • DAG(36:1)
Diglycerides Group C
  • 1-Myristoyl-3-stearoyl-sn-glycerol
  • 1-Tetradecanoyl-3-octadecanoyl-sn-glycerol
  • DAG(14:0/0:0/18:0)
  • DAG(32:0)
  • DG(32:0)
Diglycerides Group D
  • 1,2-Didocosahexaenoyl-rac-glycerol
  • DAG(22:6/22:6)
  • DAG(22:6n3/22:6n3)
  • DAG(22:6w3/22:6w3)
  • DAG(44:12)
genes like me logo Genes that share compounds with PRKCE: view

Drug Products

Transcripts for PRKCE Gene

Unigene Clusters for PRKCE Gene

Protein kinase C, epsilon:
Representative Sequences:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for PRKCE

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for PRKCE Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17 ^ 18 ^ 19a · 19b ^ 20 ^ 21a · 21b ^ 22a · 22b ^
SP1: - - - - - - - - -
SP2:
SP3: - - -
SP4:
SP5:

ExUns: 23
SP1:
SP2:
SP3:
SP4:
SP5:

Relevant External Links for PRKCE Gene

GeneLoc Exon Structure for
PRKCE
ECgene alternative splicing isoforms for
PRKCE

Expression for PRKCE Gene

mRNA expression in normal human tissues for PRKCE Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for PRKCE Gene

This gene is overexpressed in Brain - Frontal Cortex (BA9) (x5.6) and Brain - Anterior cingulate cortex (BA24) (x4.9).

Protein differential expression in normal tissues from HIPED for PRKCE Gene

This gene is overexpressed in Frontal cortex (50.2).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for PRKCE Gene



SOURCE GeneReport for Unigene cluster for PRKCE Gene Hs.580351

genes like me logo Genes that share expression patterns with PRKCE: view

Protein tissue co-expression partners for PRKCE Gene

- Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA Expression by UniProt/SwissProt for PRKCE Gene

Orthologs for PRKCE Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for PRKCE Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia PRKCE 35
  • 92.54 (n)
  • 99.19 (a)
PRKCE 36
  • 99 (a)
OneToOne
dog
(Canis familiaris)
Mammalia PRKCE 35
  • 93.17 (n)
  • 98.91 (a)
PRKCE 36
  • 99 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Prkce 35
  • 91.09 (n)
  • 97.96 (a)
Prkce 16
Prkce 36
  • 98 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia PRKCE 35
  • 99.64 (n)
  • 100 (a)
PRKCE 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Prkce 35
  • 90.86 (n)
  • 98.1 (a)
oppossum
(Monodelphis domestica)
Mammalia PRKCE 36
  • 96 (a)
OneToOne
chicken
(Gallus gallus)
Aves PRKCE 35
  • 81.95 (n)
  • 95.93 (a)
PRKCE 36
  • 95 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia PRKCE 36
  • 94 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia prkce 35
  • 79.25 (n)
  • 91.96 (a)
Str.13025 35
zebrafish
(Danio rerio)
Actinopterygii Dr.12470 35
prkceb 35
  • 76.34 (n)
  • 86.92 (a)
prkcea 36
  • 85 (a)
OneToMany
prkceb 36
  • 86 (a)
OneToMany
fruit fly
(Drosophila melanogaster)
Insecta Pkc98E 37
  • 59 (a)
Pkc98E 35
  • 60.64 (n)
  • 59.94 (a)
Pkc98E 36
  • 57 (a)
OneToMany
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP002748 35
  • 60.6 (n)
  • 60.97 (a)
worm
(Caenorhabditis elegans)
Secernentea F48G7.10 37
  • 44 (a)
R12G8.1 37
  • 43 (a)
tpa-1 37
  • 42 (a)
pkc-1 35
  • 58.64 (n)
  • 62.26 (a)