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Aliases for PRKCE Gene

Aliases for PRKCE Gene

  • Protein Kinase C Epsilon 2 3 5
  • NPKC-Epsilon 3 4
  • EC 2.7.11.13 4 61
  • PKCE 3 4
  • Protein Kinase C Epsilon Type 3
  • Protein Kinase C, Epsilon 2
  • EC 2.7.11 61

External Ids for PRKCE Gene

Previous GeneCards Identifiers for PRKCE Gene

  • GC02P045897
  • GC02P046047
  • GC02P045853
  • GC02P045790

Summaries for PRKCE Gene

Entrez Gene Summary for PRKCE Gene

  • Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq, Jul 2008]

GeneCards Summary for PRKCE Gene

PRKCE (Protein Kinase C Epsilon) is a Protein Coding gene. Among its related pathways are CCR5 Pathway in Macrophages and Development VEGF signaling via VEGFR2 - generic cascades. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is PRKCH.

UniProtKB/Swiss-Prot for PRKCE Gene

  • Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays essential roles in the regulation of multiple cellular processes linked to cytoskeletal proteins, such as cell adhesion, motility, migration and cell cycle, functions in neuron growth and ion channel regulation, and is involved in immune response, cancer cell invasion and regulation of apoptosis. Mediates cell adhesion to the extracellular matrix via integrin-dependent signaling, by mediating angiotensin-2-induced activation of integrin beta-1 (ITGB1) in cardiac fibroblasts. Phosphorylates MARCKS, which phosphorylates and activates PTK2/FAK, leading to the spread of cardiomyocytes. Involved in the control of the directional transport of ITGB1 in mesenchymal cells by phosphorylating vimentin (VIM), an intermediate filament (IF) protein. In epithelial cells, associates with and phosphorylates keratin-8 (KRT8), which induces targeting of desmoplakin at desmosomes and regulates cell-cell contact. Phosphorylates IQGAP1, which binds to CDC42, mediating epithelial cell-cell detachment prior to migration. In HeLa cells, contributes to hepatocyte growth factor (HGF)-induced cell migration, and in human corneal epithelial cells, plays a critical role in wound healing after activation by HGF. During cytokinesis, forms a complex with YWHAB, which is crucial for daughter cell separation, and facilitates abscission by a mechanism which may implicate the regulation of RHOA. In cardiac myocytes, regulates myofilament function and excitation coupling at the Z-lines, where it is indirectly associated with F-actin via interaction with COPB1. During endothelin-induced cardiomyocyte hypertrophy, mediates activation of PTK2/FAK, which is critical for cardiomyocyte survival and regulation of sarcomere length. Plays a role in the pathogenesis of dilated cardiomyopathy via persistent phosphorylation of troponin I (TNNI3). Involved in nerve growth factor (NFG)-induced neurite outgrowth and neuron morphological change independently of its kinase activity, by inhibition of RHOA pathway, activation of CDC42 and cytoskeletal rearrangement. May be involved in presynaptic facilitation by mediating phorbol ester-induced synaptic potentiation. Phosphorylates gamma-aminobutyric acid receptor subunit gamma-2 (GABRG2), which reduces the response of GABA receptors to ethanol and benzodiazepines and may mediate acute tolerance to the intoxicating effects of ethanol. Upon PMA treatment, phosphorylates the capsaicin- and heat-activated cation channel TRPV1, which is required for bradykinin-induced sensitization of the heat response in nociceptive neurons. Is able to form a complex with PDLIM5 and N-type calcium channel, and may enhance channel activities and potentiates fast synaptic transmission by phosphorylating the pore-forming alpha subunit CACNA1B (CaV2.2). In prostate cancer cells, interacts with and phosphorylates STAT3, which increases DNA-binding and transcriptional activity of STAT3 and seems to be essential for prostate cancer cell invasion. Downstream of TLR4, plays an important role in the lipopolysaccharide (LPS)-induced immune response by phosphorylating and activating TICAM2/TRAM, which in turn activates the transcription factor IRF3 and subsequent cytokines production. In differentiating erythroid progenitors, is regulated by EPO and controls the protection against the TNFSF10/TRAIL-mediated apoptosis, via BCL2. May be involved in the regulation of the insulin-induced phosphorylation and activation of AKT1.

Tocris Summary for PRKCE Gene

  • Protein kinase C (PKC) refers to a family of serine/threonine protein kinases grouped by their activation mechanism. Conventional PKCs (cPKC alpha-, betaI- , betaII- and gamma-) are activated by phosphatidylserine in a calcium dependent manner and can bind diacylglycerol.

Gene Wiki entry for PRKCE Gene

No data available for CIViC summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PRKCE Gene

Genomics for PRKCE Gene

Regulatory Elements for PRKCE Gene

Enhancers for PRKCE Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH02G046071 1.9 FANTOM5 Ensembl ENCODE dbSUPER 9.2 +423.0 423001 5.7 PKNOX1 ZFP64 ARID4B SIN3A DMAP1 YY1 ZNF766 ZNF207 FOS KLF13 EPAS1 ENSG00000231336 ENSG00000226548 LINC01119 LOC105374580 PRKCE ENSG00000232696 RPL26P15
GH02G045935 1.5 Ensembl ENCODE dbSUPER 11.6 +285.1 285132 1.3 ATF1 ZFP64 WRNIP1 ARID4B SIN3A BRCA1 ZNF2 ZNF48 ZNF766 ZNF143 PRKCE LOC105374580 GC02M046004 GC02P045738
GH02G045937 1.5 Ensembl ENCODE dbSUPER 11.6 +287.7 287721 2.5 PKNOX1 FOXA2 SIN3A FEZF1 YY1 GATA2 ZNF143 FOS ZNF263 REST PRKCE SOCS5 LOC105374580 GC02M046004 GC02P045738
GH02G046064 1.8 FANTOM5 Ensembl ENCODE dbSUPER 9.2 +415.7 415692 4.7 ATF1 SIN3A BRCA1 ZNF766 GATA2 FOS DEK ZNF263 REST MEF2D EPAS1 ENSG00000231336 ENSG00000226548 LINC01119 LOC105374580 PRKCE ENSG00000232696 RPL26P15
GH02G045722 0.9 Ensembl ENCODE 18 +71.9 71910 1.2 MXI1 BHLHE40 MAX MNT EBF1 TBX21 RAD51 BCL6B RELB MLLT1 PRKCE LOC105374580 GC02P045738 TRQ-TTG5-1
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around PRKCE on UCSC Golden Path with GeneCards custom track

Promoters for PRKCE Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000290745 -115 2801 HDGF FOXA2 ARNT WRNIP1 ARID4B SIN3A FEZF1 DMAP1 ZNF2 YY1
ENSR00000116454 1585 201 MXI1 SUZ12 MAX ZFHX2 EGR1 ZBTB20 EGR2 SP3 L3MBTL2 ZBTB8A

Genomic Location for PRKCE Gene

Chromosome:
2
Start:
45,651,315 bp from pter
End:
46,187,990 bp from pter
Size:
536,676 bases
Orientation:
Plus strand

Genomic View for PRKCE Gene

Genes around PRKCE on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PRKCE Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PRKCE Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PRKCE Gene

Proteins for PRKCE Gene

  • Protein details for PRKCE Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q02156-KPCE_HUMAN
    Recommended name:
    Protein kinase C epsilon type
    Protein Accession:
    Q02156
    Secondary Accessions:
    • B0LPH7
    • Q32MQ3
    • Q53SL4
    • Q53SM5
    • Q9UE81

    Protein attributes for PRKCE Gene

    Size:
    737 amino acids
    Molecular mass:
    83674 Da
    Quaternary structure:
    • Forms a ternary complex with TRIM63 and GN2BL1. Can form a complex with PDLIM5 and N-type calcium channel. Interacts with COPB1 and YWHAB (By similarity). Interacts with DGKQ and STAT3. Interacts with HSP90AB1; promotes functional activation in a heat shock-dependent manner (PubMed:20353823).

    Three dimensional structures from OCA and Proteopedia for PRKCE Gene

neXtProt entry for PRKCE Gene

Selected DME Specific Peptides for PRKCE Gene

Q02156:
  • VNGGDLM
  • RKFDEPR
  • VLGKGSFGKV
  • GKGSFGKV
  • ILKAFMTKN
  • SVDWWALGVLMYEMMAGQPPFEADNED
  • NVAPNCGV
  • DFGMCKE
  • MNVHRRC
  • LSKEAVS
  • YRDLKLDN
  • RQGLQCK
  • VDWWALGVL
  • DRLFFVME
  • CTMTEKR
  • TFCDHCGSLL
  • YAVKVLKKDVILQDDDV
  • FGKVMLAE
  • GTPDYIAPE
  • TFCGTPDYIAPEI
  • YIAPEIL
  • FVMEYVNGG
  • HPFFKEIDW
  • VVHKRCH
  • GAESPQP
  • NGHKFMATYLRQPTYCSHCR
  • FQTKDRL
  • RDLKLDN

Post-translational modifications for PRKCE Gene

  • Phosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729. Phosphorylation in the hinge domain at Ser-350 by MAPK11 or MAPK14, Ser-346 by GSK3B and Ser-368 by autophosphorylation is required for interaction with YWHAB.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for PRKCE (PKCE)
  • Cloud-Clone Corp. Antibodies for PRKCE

Domains & Families for PRKCE Gene

Suggested Antigen Peptide Sequences for PRKCE Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q02156

UniProtKB/Swiss-Prot:

KPCE_HUMAN :
  • The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Domain:
  • The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
Family:
  • Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
genes like me logo Genes that share domains with PRKCE: view

Function for PRKCE Gene

Molecular function for PRKCE Gene

UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation:
Novel PKCs (PRKCD, PRKCE, PRKCH and PRKCQ) are calcium-insensitive, but activated by diacylglycerol (DAG) and phosphatidylserine. Three specific sites; Thr-566 (activation loop of the kinase domain), Thr-710 (turn motif) and Ser-729 (hydrophobic region), need to be phosphorylated for its full activation.
UniProtKB/Swiss-Prot Function:
Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays essential roles in the regulation of multiple cellular processes linked to cytoskeletal proteins, such as cell adhesion, motility, migration and cell cycle, functions in neuron growth and ion channel regulation, and is involved in immune response, cancer cell invasion and regulation of apoptosis. Mediates cell adhesion to the extracellular matrix via integrin-dependent signaling, by mediating angiotensin-2-induced activation of integrin beta-1 (ITGB1) in cardiac fibroblasts. Phosphorylates MARCKS, which phosphorylates and activates PTK2/FAK, leading to the spread of cardiomyocytes. Involved in the control of the directional transport of ITGB1 in mesenchymal cells by phosphorylating vimentin (VIM), an intermediate filament (IF) protein. In epithelial cells, associates with and phosphorylates keratin-8 (KRT8), which induces targeting of desmoplakin at desmosomes and regulates cell-cell contact. Phosphorylates IQGAP1, which binds to CDC42, mediating epithelial cell-cell detachment prior to migration. In HeLa cells, contributes to hepatocyte growth factor (HGF)-induced cell migration, and in human corneal epithelial cells, plays a critical role in wound healing after activation by HGF. During cytokinesis, forms a complex with YWHAB, which is crucial for daughter cell separation, and facilitates abscission by a mechanism which may implicate the regulation of RHOA. In cardiac myocytes, regulates myofilament function and excitation coupling at the Z-lines, where it is indirectly associated with F-actin via interaction with COPB1. During endothelin-induced cardiomyocyte hypertrophy, mediates activation of PTK2/FAK, which is critical for cardiomyocyte survival and regulation of sarcomere length. Plays a role in the pathogenesis of dilated cardiomyopathy via persistent phosphorylation of troponin I (TNNI3). Involved in nerve growth factor (NFG)-induced neurite outgrowth and neuron morphological change independently of its kinase activity, by inhibition of RHOA pathway, activation of CDC42 and cytoskeletal rearrangement. May be involved in presynaptic facilitation by mediating phorbol ester-induced synaptic potentiation. Phosphorylates gamma-aminobutyric acid receptor subunit gamma-2 (GABRG2), which reduces the response of GABA receptors to ethanol and benzodiazepines and may mediate acute tolerance to the intoxicating effects of ethanol. Upon PMA treatment, phosphorylates the capsaicin- and heat-activated cation channel TRPV1, which is required for bradykinin-induced sensitization of the heat response in nociceptive neurons. Is able to form a complex with PDLIM5 and N-type calcium channel, and may enhance channel activities and potentiates fast synaptic transmission by phosphorylating the pore-forming alpha subunit CACNA1B (CaV2.2). In prostate cancer cells, interacts with and phosphorylates STAT3, which increases DNA-binding and transcriptional activity of STAT3 and seems to be essential for prostate cancer cell invasion. Downstream of TLR4, plays an important role in the lipopolysaccharide (LPS)-induced immune response by phosphorylating and activating TICAM2/TRAM, which in turn activates the transcription factor IRF3 and subsequent cytokines production. In differentiating erythroid progenitors, is regulated by EPO and controls the protection against the TNFSF10/TRAIL-mediated apoptosis, via BCL2. May be involved in the regulation of the insulin-induced phosphorylation and activation of AKT1.

Enzyme Numbers (IUBMB) for PRKCE Gene

Gene Ontology (GO) - Molecular Function for PRKCE Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003785 actin monomer binding ISS --
GO:0004672 protein kinase activity IEA --
GO:0004674 protein serine/threonine kinase activity IDA 15695813
GO:0004697 protein kinase C activity TAS,IDA --
GO:0004699 calcium-independent protein kinase C activity IEA --
genes like me logo Genes that share ontologies with PRKCE: view
genes like me logo Genes that share phenotypes with PRKCE: view

Animal Models for PRKCE Gene

MGI Knock Outs for PRKCE:

Animal Model Products

Inhibitory RNA Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for PRKCE Gene

Localization for PRKCE Gene

Subcellular locations from UniProtKB/Swiss-Prot for PRKCE Gene

Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cytoplasm, perinuclear region. Nucleus. Note=Translocated to plasma membrane in epithelial cells stimulated by HGF. Associated with the Golgi at the perinuclear site in pre-passage fibroblasts (By similarity). In passaging cells, translocated to the cell periphery (By similarity). Translocated to the nucleus in PMA-treated cells (By similarity). {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for PRKCE gene
Compartment Confidence
plasma membrane 5
endoplasmic reticulum 5
cytosol 5
nucleus 4
cytoskeleton 3
mitochondrion 3
golgi apparatus 2

Gene Ontology (GO) - Cellular Components for PRKCE Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005737 cytoplasm IDA 15632189
GO:0005739 mitochondrion IEA --
GO:0005783 endoplasmic reticulum IDA 18556656
GO:0005794 colocalizes_with Golgi apparatus ISS --
genes like me logo Genes that share ontologies with PRKCE: view

Pathways & Interactions for PRKCE Gene

genes like me logo Genes that share pathways with PRKCE: view

Pathways by source for PRKCE Gene

SIGNOR curated interactions for PRKCE Gene

Activates:
Inactivates:
Is activated by:
Is inactivated by:

Gene Ontology (GO) - Biological Process for PRKCE Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0002281 macrophage activation involved in immune response IEA --
GO:0002376 immune system process IEA --
GO:0006468 protein phosphorylation IEA,IDA 18556656
GO:0006915 apoptotic process TAS 10438519
GO:0007049 cell cycle IEA --
genes like me logo Genes that share ontologies with PRKCE: view

Drugs & Compounds for PRKCE Gene

(38) Drugs for PRKCE Gene - From: DrugBank, PharmGKB, ApexBio, DGIdb, FDA Approved Drugs, HMDB, Tocris, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Tamoxifen Approved Pharma Antagonist, Target TGF-β modulatory and PKC inhibitory effects, ER antagonist, Anti-Estrogens 390
Ingenol Mebutate Approved Pharma 0
nolvadex Approved October 1998 Pharma 0
Adenosine triphosphate Approved Nutra 0
Staurosporine Experimental Pharma Positive, Allosteric regulator Protein kinase inhibitor,potent and cell permeable 47

(12) Additional Compounds for PRKCE Gene - From: Novoseek and HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Full agonist, Agonist 58-64-0