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Aliases for POLR2B Gene

Aliases for POLR2B Gene

  • Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa 2 3
  • DNA-Directed RNA Polymerase II 140 KDa Polypeptide 3 4
  • DNA-Directed RNA Polymerase II Subunit B 3 4
  • RNA Polymerase II Subunit B2 3 4
  • RNA Polymerase II Subunit 2 3 4
  • EC 2.7.7.6 4 64
  • POL2RB 3 6
  • Polymerase (RNA) II (DNA Directed) Polypeptide B (140kD) 2
  • DNA-Directed RNA Polymerase II Subunit RPB2 3
  • RNA Polymerase II Second Largest Subunit 3
  • EC 2.7.7 64
  • HRPB140 3
  • RPB2 3

External Ids for POLR2B Gene

Summaries for POLR2B Gene

Entrez Gene Summary for POLR2B Gene

  • This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

GeneCards Summary for POLR2B Gene

POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa) is a Protein Coding gene. Diseases associated with POLR2B include eumycotic mycetoma and hiv-1. Among its related pathways are Disease and GPCR Pathway. GO annotations related to this gene include chromatin binding and ribonucleoside binding. An important paralog of this gene is POLR1B.

UniProtKB/Swiss-Prot for POLR2B Gene

  • DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).

Gene Wiki entry for POLR2B Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for POLR2B Gene

Genomics for POLR2B Gene

Genomic Location for POLR2B Gene

Start:
56,977,722 bp from pter
End:
57,031,168 bp from pter
Size:
53,447 bases
Orientation:
Plus strand

Genomic View for POLR2B Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for POLR2B Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for POLR2B Gene

Regulatory Elements for POLR2B Gene

Proteins for POLR2B Gene

  • Protein details for POLR2B Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P30876-RPB2_HUMAN
    Recommended name:
    DNA-directed RNA polymerase II subunit RPB2
    Protein Accession:
    P30876
    Secondary Accessions:
    • A8K1A8
    • Q8IZ61

    Protein attributes for POLR2B Gene

    Size:
    1174 amino acids
    Molecular mass:
    133897 Da
    Quaternary structure:
    • Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. Interacts with WDR82. Interacts with MEN1.
    Miscellaneous:
    • The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits (By similarity).

neXtProt entry for POLR2B Gene

Proteomics data for POLR2B Gene at MOPED

Post-translational modifications for POLR2B Gene

  • Modification sites at PhosphoSitePlus
  • Ubiquitination at Lys95, Lys130, Lys134, Lys146, Lys151, Lys199, Lys214, Lys264, Lys340, Lys421, Lys445, Lys457, Lys578, Lys730, Lys756, Lys821, Lys831, Lys847, Lys869, Lys917, Lys934, Lys942, Lys988, Lys993, Lys1010, Lys1036, Lys1143, and Lys1156

Other Protein References for POLR2B Gene

Domains for POLR2B Gene

Gene Families for POLR2B Gene

HGNC:
  • POLR :RNA polymerase subunits

UniProtKB/Swiss-Prot:

RPB2_HUMAN
Family:
  • Belongs to the RNA polymerase beta chain family.:
    • P30876
genes like me logo Genes that share domains with POLR2B: view

Function for POLR2B Gene

Molecular function for POLR2B Gene

GENATLAS Biochemistry: polymerase II,RNA (DNA directed) large polypeptide B (140kDa)
UniProtKB/Swiss-Prot CatalyticActivity: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
UniProtKB/Swiss-Prot Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).

Enzyme Numbers (IUBMB) for POLR2B Gene

Gene Ontology (GO) - Molecular Function for POLR2B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001055 contributes_to RNA polymerase II activity IBA --
GO:0003677 DNA binding IEA --
GO:0003682 chromatin binding IEA --
GO:0003899 DNA-directed RNA polymerase activity --
GO:0005515 protein binding IPI 17998332
genes like me logo Genes that share ontologies with POLR2B: view
genes like me logo Genes that share phenotypes with POLR2B: view

miRNA for POLR2B Gene

No data available for Animal Models , Transcription Factor Targeting and HOMER Transcription for POLR2B Gene

Localization for POLR2B Gene

Subcellular locations from UniProtKB/Swiss-Prot for POLR2B Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for POLR2B Gene COMPARTMENTS Subcellular localization image for POLR2B gene
Compartment Confidence
nucleus 5
cytosol 2
cytoskeleton 1

Gene Ontology (GO) - Cellular Components for POLR2B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA 9852112
GO:0005654 nucleoplasm TAS --
GO:0005665 DNA-directed RNA polymerase II, core complex IDA 9852112
GO:0016020 membrane IDA 19946888
genes like me logo Genes that share ontologies with POLR2B: view

Pathways for POLR2B Gene

SuperPathways for POLR2B Gene

Superpath Contained pathways
1 Formation of RNA Pol II elongation complex
2 DNA Repair
3 Assembly of RNA Polymerase-II Initiation Complex
4 mRNA Splicing - Major Pathway
5 HIV Life Cycle
genes like me logo Genes that share pathways with POLR2B: view

Gene Ontology (GO) - Biological Process for POLR2B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000398 mRNA splicing, via spliceosome TAS --
GO:0006281 DNA repair TAS --
GO:0006283 transcription-coupled nucleotide-excision repair TAS --
GO:0006289 nucleotide-excision repair TAS --
GO:0006351 transcription, DNA-templated --
genes like me logo Genes that share ontologies with POLR2B: view

Compounds for POLR2B Gene

(8) HMDB Compounds for POLR2B Gene

Compound Synonyms Cas Number PubMed IDs
Adenosine triphosphate
  • 5'-(tetrahydrogen triphosphate) Adenosine
56-65-5
Cytidine triphosphate
  • cytidine 5'-(tetrahydrogen triphosphate)
65-47-4
Guanosine triphosphate
  • 5'-GTP
86-01-1
Magnesium
  • Magnesium
7439-95-4
Phosphoric acid
  • acide phosphorique (FRENCH)
7664-38-2
genes like me logo Genes that share compounds with POLR2B: view

Transcripts for POLR2B Gene

Unigene Clusters for POLR2B Gene

Polymerase (RNA) II (DNA directed) polypeptide B, 140kDa:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for POLR2B Gene

ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3a · 3b ^ 4a · 4b ^ 5a · 5b · 5c ^ 6a · 6b ^ 7a · 7b · 7c ^ 8 ^ 9a · 9b ^ 10a · 10b ^ 11a · 11b · 11c ^ 12a ·
SP1: -
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:
SP12:
SP13: - - -
SP14: - - -
SP15:

ExUns: 12b ^ 13 ^ 14 ^ 15 ^ 16a · 16b ^ 17a · 17b ^ 18a · 18b ^ 19 ^ 20 ^ 21a · 21b ^ 22a · 22b ^ 23a · 23b ^ 24a · 24b ^ 25 ^ 26
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:
SP12:
SP13:
SP14:
SP15:

Relevant External Links for POLR2B Gene

GeneLoc Exon Structure for
POLR2B
ECgene alternative splicing isoforms for
POLR2B

Expression for POLR2B Gene

mRNA expression in normal human tissues for POLR2B Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for POLR2B Gene

SOURCE GeneReport for Unigene cluster for POLR2B Gene Hs.602757

genes like me logo Genes that share expressions with POLR2B: view

Orthologs for POLR2B Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for POLR2B Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia POLR2B 36
  • 99.66 (n)
  • 100 (a)
POLR2B 37
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia POLR2B 36
  • 94.32 (n)
  • 99.91 (a)
POLR2B 37
  • 100 (a)
OneToOne
dog
(Canis familiaris)
Mammalia POLR2B 36
  • 94.92 (n)
  • 99.91 (a)
POLR2B 37
  • 100 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Polr2b 36
  • 90.35 (n)
  • 99.91 (a)
Polr2b 16
Polr2b 37
  • 100 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia POLR2B 37
  • 100 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia -- 37
  • 100 (a)
OneToMany
-- 37
  • 81 (a)
OneToMany
rat
(Rattus norvegicus)
Mammalia Polr2b 36
  • 89.89 (n)
  • 100 (a)
chicken
(Gallus gallus)
Aves POLR2B 36
  • 83.82 (n)
  • 99.4 (a)
POLR2B 37
  • 99 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia POLR2B 37
  • 99 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia MGC68569 36
tropical clawed frog
(Silurana tropicalis)
Amphibia polr2b 36
  • 82.61 (n)
  • 99.06 (a)
Str.15006 36
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.13862 36
zebrafish
(Danio rerio)
Actinopterygii Dr.16327 36
polr2b 36
  • 77.98 (n)
  • 97.27 (a)
polr2b 37
  • 96 (a)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP003648 36
  • 69.31 (n)
  • 86.35 (a)
fruit fly
(Drosophila melanogaster)
Insecta RpII140 36
  • 72.35 (n)
  • 86.18 (a)
RpII140 37
  • 85 (a)
OneToOne
RpIII128 38
  • 36 (a)
RpI135 38
  • 27 (a)
RpII140 38
  • 86 (a)
worm
(Caenorhabditis elegans)
Secernentea rpb-2 36
  • 70.2 (n)
  • 79.13 (a)
rpb-2 37
  • 77 (a)
OneToOne
C26E6.4 38
  • 78 (a)
F09F7.3 38
  • 36 (a)
F14B4.3 38
  • 29 (a)
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_AFR404C 36
  • 60.06 (n)
  • 60.86 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes RPB2 36
  • 61.81 (n)
  • 61.29 (a)
RPB2 37
  • 56 (a)
OneToMany
RPB2 39
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0B05577g 36
  • 61.35 (n)
  • 61.18 (a)
Alicante grape
(Vitis vinifera)
eudicotyledons Vvi.3309 36
thale cress
(Arabidopsis thaliana)
eudicotyledons NRPB2 36
  • 64.57 (n)
  • 68.04 (a)
barley
(Hordeum vulgare)
Liliopsida Hv.4411 36
rice
(Oryza sativa)
Liliopsida Os.25542 36
Os03g0646800 36
  • 64.35 (n)
  • 69.28 (a)
wheat
(Triticum aestivum)
Liliopsida Ta.12287 36
bread mold
(Neurospora crassa)
Ascomycetes NCU05120 36
  • 59.4 (n)
  • 62.59 (a)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes rpb2 36
  • 62.59 (n)
  • 63.69 (a)
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.11524 36
sea squirt
(Ciona savignyi)
Ascidiacea CSA.1138 37
  • 82 (a)
OneToOne
Species with no ortholog for POLR2B:
  • Actinobacteria (Mycobacterium tuberculosis)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)

Evolution for POLR2B Gene

ENSEMBL:
Gene Tree for POLR2B (if available)
TreeFam:
Gene Tree for POLR2B (if available)

Paralogs for POLR2B Gene

Paralogs for POLR2B Gene

Selected SIMAP similar genes for POLR2B Gene using alignment to 5 proteins:

genes like me logo Genes that share paralogs with POLR2B: view

Variants for POLR2B Gene

Sequence variations from dbSNP and Humsavar for POLR2B Gene

SNP ID Clin Chr 04 pos Sequence Context AA Info Type MAF
rs1277279 -- 56,999,237(+) CTCTC(A/T)TCTAT intron-variant
rs1277280 -- 57,000,706(+) TTTTT(G/T)GGGGG intron-variant
rs1277281 -- 56,989,926(-) AAAAG(C/T)AGACA intron-variant
rs1277282 -- 56,989,653(-) GAGAC(C/T)TTCTT intron-variant
rs1277283 -- 56,989,475(-) ACCAA(A/T)ATTAG intron-variant

Relevant External Links for POLR2B Gene

HapMap Linkage Disequilibrium report
POLR2B

No data available for Structural Variations from Database of Genomic Variants (DGV) for POLR2B Gene

Disorders for POLR2B Gene

(1) University of Copenhagen DISEASES for POLR2B Gene

Relevant External Links for POLR2B

Genetic Association Database (GAD)
POLR2B
Human Genome Epidemiology (HuGE) Navigator
POLR2B
genes like me logo Genes that share disorders with POLR2B: view

No data available for UniProtKB/Swiss-Prot for POLR2B Gene

Publications for POLR2B Gene

  1. Primary structure of the second largest subunit of human RNA polymerase II (or B). (PMID: 1518060) Acker J. … Kedinger C. (J. Mol. Biol. 1992) 2 3 4
  2. Chromosomal localization of human RNA polymerase II subunit genes. (PMID: 8034326) Acker J. … Kedinger C. (Genomics 1994) 2 3
  3. Large-scale concatenation cDNA sequencing. (PMID: 9110174) Yu W. … Gibbs R.A. (Genome Res. 1997) 3 4
  4. Identification of proteins interacting with the RNAPII FCP1 phosphatase: FCP1 forms a complex with arginine methyltransferase PRMT5 and it is a substrate for PRMT5-mediated methylation. (PMID: 15670829) Amente S. … Majello B. (FEBS Lett. 2005) 3 23
  5. Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes. (PMID: 9852112) Kershnar E. … Chiang C.-M. (J. Biol. Chem. 1998) 3 4

Products for POLR2B Gene

  • antibodies-online peptides for POLR2B
  • antibodies-online antibodies for POLR2B
  • antibodies-online kits for POLR2B

Sources for POLR2B Gene

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