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Aliases for DPH5 Gene

Aliases for DPH5 Gene

  • Diphthamide Biosynthesis 5 2 3 5
  • Diphthamide Biosynthesis Methyltransferase 3 4
  • Diphthine Methyl Ester Synthase 3
  • DPH5 Homolog (S. Cerevisiae) 2
  • Diphthine Synthase 3
  • Protein X 0011 3
  • DPH5 Homolog 3
  • EC 4
  • EC 61
  • HSPC143 3
  • AD-018 3
  • CGI-30 3
  • NPD015 3

External Ids for DPH5 Gene

Previous GeneCards Identifiers for DPH5 Gene

  • GC01M101168
  • GC01M101227
  • GC01M101455
  • GC01M099580

Summaries for DPH5 Gene

Entrez Gene Summary for DPH5 Gene

  • This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

GeneCards Summary for DPH5 Gene

DPH5 (Diphthamide Biosynthesis 5) is a Protein Coding gene. Among its related pathways are Metabolism of proteins and Gamma carboxylation, hypusine formation and arylsulfatase activation. GO annotations related to this gene include methyltransferase activity and diphthine synthase activity.

UniProtKB/Swiss-Prot for DPH5 Gene

  • S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.

Gene Wiki entry for DPH5 Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for DPH5 Gene

Genomics for DPH5 Gene

Regulatory Elements for DPH5 Gene

Enhancers for DPH5 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH01G101257 1.9 FANTOM5 Ensembl ENCODE dbSUPER 10.2 -235.6 -235558 9.1 PKNOX1 FOXA2 YBX1 FEZF1 ZNF2 YY1 ZNF766 FOS SP5 SREBF1 SLC30A7 HNRNPA1P68 ENSG00000235795 ENSG00000252765 DPH5 LOC102606465 GC01M101263 RPS20P6
GH01G101110 1.6 FANTOM5 Ensembl ENCODE dbSUPER 11.3 -85.7 -85704 2.9 BRD4 ATF1 ZBTB40 FOXM1 ZNF766 ARID3A GATA2 CBX5 EED ETV6 LOC102606465 ENSG00000252765 DPH5 S1PR1 SLC30A7 GC01P101108 GC01P101137
GH01G101234 1.6 Ensembl ENCODE dbSUPER 11.2 -215.4 -215446 14.7 PKNOX1 WRNIP1 FEZF1 ZNF2 YY1 CBX5 ZNF143 FOS ZNF263 SP3 ENSG00000252765 DPH5 LOC102606465 LOC101928370 GC01M101239 GC01M101240 ENSG00000260940
GH01G101276 1.5 FANTOM5 ENCODE dbSUPER 11 -255.1 -255089 8.8 PKNOX1 ATF1 ARNT FEZF1 TCF12 ZNF766 GATA2 FOS ZNF548 SP5 HNRNPA1P68 ENSG00000252765 DPH5 LOC102606465 SLC30A7 S1PR1 PPIAP7 LOC102723784
GH01G101135 1.4 Ensembl ENCODE dbSUPER 10.5 -111.0 -111005 2.3 HDAC1 FOXA2 THRB RAD21 RARA YY1 GATA2 CBX5 SCRT2 CREM DPH5 LOC102606465 SLC30A7 ENSG00000252765 LOC101928334
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around DPH5 on UCSC Golden Path with GeneCards custom track

Promoters for DPH5 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

Genomic Location for DPH5 Gene

100,989,623 bp from pter
101,026,088 bp from pter
36,466 bases
Minus strand

Genomic View for DPH5 Gene

Genes around DPH5 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
DPH5 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for DPH5 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for DPH5 Gene

Proteins for DPH5 Gene

  • Protein details for DPH5 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Diphthine methyl ester synthase
    Protein Accession:
    Secondary Accessions:
    • A8JZY6
    • D3DT62
    • Q9P017
    • Q9P0I4
    • Q9Y319

    Protein attributes for DPH5 Gene

    285 amino acids
    Molecular mass:
    31651 Da
    Quaternary structure:
    No Data Available

    Alternative splice isoforms for DPH5 Gene

neXtProt entry for DPH5 Gene

Selected DME Specific Peptides for DPH5 Gene


Post-translational modifications for DPH5 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Domains & Families for DPH5 Gene

Protein Domains for DPH5 Gene

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the diphthine synthase family.
  • Belongs to the diphthine synthase family.
genes like me logo Genes that share domains with DPH5: view

No data available for Gene Families for DPH5 Gene

Function for DPH5 Gene

Molecular function for DPH5 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
4 S-adenosyl-L-methionine + 2-((3S)-3-carboxy-3-aminopropyl)-L-histidine-[translation elongation factor 2] = 4 S-adenosyl-L-homocysteine + diphthine methyl ester-[translation elongation factor 2].
UniProtKB/Swiss-Prot Function:
S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.

Enzyme Numbers (IUBMB) for DPH5 Gene

Gene Ontology (GO) - Molecular Function for DPH5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004164 diphthine synthase activity NAS 15485916
GO:0008168 methyltransferase activity IEA --
GO:0016740 transferase activity IEA --
genes like me logo Genes that share ontologies with DPH5: view

Phenotypes for DPH5 Gene

GenomeRNAi human phenotypes for DPH5:
genes like me logo Genes that share phenotypes with DPH5: view

Animal Model Products

CRISPR Products

miRNA for DPH5 Gene

miRTarBase miRNAs that target DPH5

Inhibitory RNA Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for DPH5 Gene

Localization for DPH5 Gene

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for DPH5 gene
Compartment Confidence
cytosol 5
nucleus 2

Gene Ontology (GO) - Cellular Components for DPH5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005575 cellular_component ND --
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with DPH5: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot for DPH5 Gene

Pathways & Interactions for DPH5 Gene

genes like me logo Genes that share pathways with DPH5: view

UniProtKB/Swiss-Prot Q9H2P9-DPH5_HUMAN

  • Pathway: Protein modification; peptidyl-diphthamide biosynthesis.

Gene Ontology (GO) - Biological Process for DPH5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0008152 metabolic process IEA --
GO:0017183 peptidyl-diphthamide biosynthetic process from peptidyl-histidine TAS --
GO:0032259 methylation IEA --
genes like me logo Genes that share ontologies with DPH5: view

No data available for SIGNOR curated interactions for DPH5 Gene

Drugs & Compounds for DPH5 Gene

(2) Drugs for DPH5 Gene - From: DrugBank and HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
S-Adenosylmethionine Approved Nutra 0
s-adenosylhomocysteine Experimental Pharma Target 0

(3) Additional Compounds for DPH5 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
  • 1-Carboxy-3-(L-histidin-2-yl)-N-methylpropan-1-aminium
  • 2-[3-Carboxy-3-(methylammonio)propyl]-His
  • 2-[3-Carboxy-3-(methylammonio)propyl]-histidine
  • [3-[[4-(2-amino-2-carboxy-ethyl)-1H-imidazol-2-yl]]-1-carboxy- propyl]-methyl-ammonium
  • 2-(3-Amino-3-carboxypropyl)-L-histidine
  • 2-Amino-4-[[4-(2-amino-2-carboxy-ethyl)-1H-imidazol-2-yl]] butanoate
  • 2-Amino-4-[[4-(2-amino-2-carboxy-ethyl)-1H-imidazol-2-yl]] butanoic acid
genes like me logo Genes that share compounds with DPH5: view

Transcripts for DPH5 Gene

Unigene Clusters for DPH5 Gene

DPH5 homolog (S. cerevisiae):
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Alternative Splicing Database (ASD) splice patterns (SP) for DPH5 Gene

ExUns: 1a · 1b ^ 2a · 2b · 2c · 2d · 2e · 2f ^ 3a · 3b ^ 4 ^ 5a · 5b ^ 6a · 6b ^ 7 ^ 8a · 8b ^ 9 ^ 10a · 10b ^ 11
SP1: - - - - -
SP2: - - - - - -
SP3: - - - - - -
SP4: - - - - - - -
SP5: - - - - - - -
SP6: - - - - - - - -
SP7: - - - - -
SP8: - -
SP9: - - - -
SP10: - - - - - - - -
SP11: - - - - - - -
SP12: - -
SP13: - -

Relevant External Links for DPH5 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for DPH5 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for DPH5 Gene

Protein differential expression in normal tissues from HIPED for DPH5 Gene

This gene is overexpressed in Monocytes (10.4).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for DPH5 Gene

Protein tissue co-expression partners for DPH5 Gene

NURSA nuclear receptor signaling pathways regulating expression of DPH5 Gene:


SOURCE GeneReport for Unigene cluster for DPH5 Gene:


Evidence on tissue expression from TISSUES for DPH5 Gene

  • Adrenal gland(4.3)
  • Nervous system(4.3)
  • Blood(4.1)
  • Eye(4.1)
genes like me logo Genes that share expression patterns with DPH5: view

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for DPH5 Gene

Orthologs for DPH5 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for DPH5 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia DPH5 34 35
  • 99.3 (n)
(Bos Taurus)
Mammalia DPH5 34 35
  • 92.16 (n)
(Canis familiaris)
Mammalia DPH5 34 35
  • 91.64 (n)
(Rattus norvegicus)
Mammalia Dph5 34
  • 88.14 (n)
(Mus musculus)
Mammalia Dph5 34 16 35
  • 87.19 (n)
(Ornithorhynchus anatinus)
Mammalia DPH5 35
  • 83 (a)
(Monodelphis domestica)
Mammalia DPH5 35
  • 79 (a)
(Gallus gallus)
Aves gga-mir-1610 35
  • 79 (a)
DPH5 34
  • 76.16 (n)
(Anolis carolinensis)
Reptilia DPH5 35
  • 77 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia dph5 34
  • 68.8 (n)
Str.7197 34
African clawed frog
(Xenopus laevis)
Amphibia cgi-30-prov 34
(Danio rerio)
Actinopterygii dph5 34 35
  • 69.96 (n)
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.13925 34
fruit fly
(Drosophila melanogaster)
Insecta Dph5 34 35
  • 59.9 (n)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP004412 34
  • 59.78 (n)
(Caenorhabditis elegans)
Secernentea B0491.7 34 35
  • 61.09 (n)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes DPH5 34 35
  • 58.43 (n)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0F22836g 34
  • 57.52 (n)
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_AFR123W 34
  • 55.56 (n)
thale cress
(Arabidopsis thaliana)
eudicotyledons AT4G31790 34
  • 63.77 (n)
(Oryza sativa)
Liliopsida Os03g0355800 34
  • 58.8 (n)
sea squirt
(Ciona savignyi)
Ascidiacea -- 35
  • 65 (a)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes dph5 34
  • 58.58 (n)
bread mold
(Neurospora crassa)
Ascomycetes NCU07266 34
  • 55.85 (n)
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.14906 34
Species where no ortholog for DPH5 was found in the sources mined by GeneCards:
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for DPH5 Gene

Gene Tree for DPH5 (if available)
Gene Tree for DPH5 (if available)

Paralogs for DPH5 Gene

No data available for Paralogs for DPH5 Gene

Variants for DPH5 Gene

Sequence variations from dbSNP and Humsavar for DPH5 Gene

SNP ID Clin Chr 01 pos Sequence Context AA Info Type
rs1000000812 -- 100,996,675(+) GGCAA(C/T)GTAAG intron-variant, nc-transcript-variant, utr-variant-3-prime
rs1000150437 -- 101,007,620(+) TGAGG(C/T)AGGAG intron-variant
rs1000181166 -- 101,001,978(+) GAGAC(C/T)ATCAA intron-variant
rs1000399962 -- 101,006,162(+) TTGAC(C/T)TCAAA intron-variant
rs1000440778 -- 100,998,976(+) GGCAT(A/G)AAAGT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for DPH5 Gene

Variant ID Type Subtype PubMed ID
esv2668878 CNV deletion 23128226
esv3586958 CNV loss 21293372
esv3586959 CNV loss 21293372
esv3586960 CNV gain 21293372
nsv522064 CNV gain 19592680
nsv950523 CNV duplication 24416366
nsv998086 CNV gain 25217958

Variation tolerance for DPH5 Gene

Residual Variation Intolerance Score: 48.7% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.00; 36.92% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for DPH5 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for DPH5 Gene

Disorders for DPH5 Gene

Relevant External Links for DPH5

Genetic Association Database (GAD)
Atlas of Genetics and Cytogenetics in Oncology and Haematology:

No disorders were found for DPH5 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for DPH5 Gene

Publications for DPH5 Gene

  1. A modified form of diphthamide causes immunotoxin resistance in a lymphoma cell line with a deletion of the WDR85 gene. (PMID: 23486472) Wei H. … Pastan I. (J. Biol. Chem. 2013) 2 3 4 64
  2. Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2. (PMID: 15485916) Liu S. … Leppla S.H. (Mol. Cell. Biol. 2004) 2 3 22 64
  3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard D.S. … Malek J. (Genome Res. 2004) 3 4 64
  4. Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics. (PMID: 10810093) Lai C.-H. … Lin W.-C. (Genome Res. 2000) 3 4 64
  5. Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning. (PMID: 10931946) Hu R.-M. … Chen J.-L. (Proc. Natl. Acad. Sci. U.S.A. 2000) 3 4 64

Products for DPH5 Gene

Sources for DPH5 Gene

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