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Aliases for PAK1 Gene

Aliases for PAK1 Gene

  • P21 Protein (Cdc42/Rac)-Activated Kinase 1 2 3
  • P21/Cdc42/Rac1-Activated Kinase 1 (STE20 Homolog, Yeast) 2 3
  • P21/Cdc42/Rac1-Activated Kinase 1 (Yeast Ste20-Related) 2 3
  • EC 2.7.11.1 4 63
  • Alpha-PAK 3 4
  • P65-PAK 3 4
  • Serine/Threonine-Protein Kinase PAK 1 3
  • P21-Activated Kinase 1 4
  • STE20 Homolog, Yeast 3
  • STE20 Homolog 2
  • EC 2.7.11 63
  • PAKalpha 3
  • PAK-1 4
  • Yeast 2

External Ids for PAK1 Gene

Previous GeneCards Identifiers for PAK1 Gene

  • GC11M079360
  • GC11M078573
  • GC11M076760
  • GC11M076710
  • GC11M077033
  • GC11M073331

Summaries for PAK1 Gene

Entrez Gene Summary for PAK1 Gene

  • This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]

GeneCards Summary for PAK1 Gene

PAK1 (P21 Protein (Cdc42/Rac)-Activated Kinase 1) is a Protein Coding gene. Among its related pathways are PI-3K cascade and MAPK signaling pathway. GO annotations related to this gene include identical protein binding and protein kinase binding. An important paralog of this gene is PAK7.

UniProtKB/Swiss-Prot for PAK1 Gene

  • Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases. Involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes. Phosphorylates the tubulin chaperone TBCB and thereby plays a role in the regulation of microtubule biogenesis and organization of the tubulin cytoskeleton. Plays a role in the regulation of insulin secretion in response to elevated glucose levels. Part of a ternary complex that contains PAK1, DVL1 and MUSK that is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Phosphorylates MYL9/MLC2. Phosphorylates RAF1 at Ser-338 and Ser-339 resulting in: activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2. Phosphorylates SNAI1 at Ser-246 promoting its transcriptional repressor activity by increasing its accumulation in the nucleus. In podocytes, promotes NR3C2 nuclear localization. Required for atypical chemokine receptor ACKR2-induced phosphorylation of LIMK1 and cofilin (CFL1) and for the up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3, maybe through CFL1 phosphorylation and inactivation.

Tocris Summary for PAK1 Gene

Gene Wiki entry for PAK1 Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PAK1 Gene

Genomics for PAK1 Gene

Regulatory Elements for PAK1 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for PAK1 Gene

Start:
77,321,707 bp from pter
End:
77,474,635 bp from pter
Size:
152,929 bases
Orientation:
Minus strand

Genomic View for PAK1 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for PAK1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PAK1 Gene

Proteins for PAK1 Gene

  • Protein details for PAK1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q13153-PAK1_HUMAN
    Recommended name:
    Serine/threonine-protein kinase PAK 1
    Protein Accession:
    Q13153
    Secondary Accessions:
    • O75561
    • Q13567
    • Q32M53
    • Q32M54
    • Q86W79

    Protein attributes for PAK1 Gene

    Size:
    545 amino acids
    Molecular mass:
    60647 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Homodimer in its autoinhibited state. Active as monomer. Component of cytoplasmic complexes, which also contains PXN, ARHGEF6 and GIT1. Interacts with NISCH (By similarity). Interacts with DVL1; mediates the formation of a DVL1, MUSK and PAK1 ternary complex involved in AChR clustering (By similarity). Binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. Interacts with ARHGEF7. Interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. Probably found in a ternary complex composed of DSCAM, PAK1 and RAC1. Interacts with DSCAM (via cytoplasmic domain); the interaction is direct and enhanced in presence of RAC1. Interacts with SCRIB. Interacts with PDPK1. Interacts (via kinase domain) with RAF1. Interacts with NCK1 and NCK2. Interacts with TBCB. Interacts with CRIPAK. Interacts with BRSK2. Interacts with SNAI1. Interacts with CIB1 isoform 2. Interacts with CIB1 (via N-terminal region); the interaction is direct, promotes PAK1 activity and occurs in a calcium-dependent manner.

    Three dimensional structures from OCA and Proteopedia for PAK1 Gene

    Alternative splice isoforms for PAK1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for PAK1 Gene

Proteomics data for PAK1 Gene at MOPED

Post-translational modifications for PAK1 Gene

  • Autophosphorylated in trans, meaning that in a dimer, one kinase molecule phosphorylates the other one. Activated by autophosphorylation at Thr-423 in response to a conformation change, triggered by interaction with GTP-bound CDC42 or RAC1. Activated by phosphorylation at Thr-423 by BRSK2 and by PDPK1. Phosphorylated by JAK2 in response to PRL; this increases PAK1 kinase activity. Phosphorylated at Ser-21 by PKB/AKT; this reduces interaction with NCK1 and association with focal adhesion sites.
  • Ubiquitination at Lys420
  • Modification sites at PhosphoSitePlus

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for PAK1 (PAK1)

Domains for PAK1 Gene

Gene Families for PAK1 Gene

Suggested Antigen Peptide Sequences for PAK1 Gene

Graphical View of Domain Structure for InterPro Entry

Q13153

UniProtKB/Swiss-Prot:

PAK1_HUMAN :
  • Q13153
Domain:
  • Contains 1 CRIB domain.
  • Contains 1 protein kinase domain.
Family:
  • Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
genes like me logo Genes that share domains with PAK1: view

Function for PAK1 Gene

Molecular function for PAK1 Gene

GENATLAS Biochemistry: p21/Cdc42/Rac1-activated kinase 1,S cerevisiae Ste20 related,expressed in brain,downstream effector linking Rho,GTPases to actin cytoskeleton,potentially involved in the regulation of a subset of cytoskeletal dynamics initiated by chemoattractant and phagocytic stimuli in human neutrophils
UniProtKB/Swiss-Prot CatalyticActivity: ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation: Phosphorylation of Thr-84 by OXSR1 inhibits activation (By similarity). Activated by binding small G proteins. Binding of GTP-bound CDC42 or RAC1 to the autoregulatory region releases monomers from the autoinhibited dimer, and enables activation by phosphorylation of Thr-423.
UniProtKB/Swiss-Prot Function: Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases. Involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes. Phosphorylates the tubulin chaperone TBCB and thereby plays a role in the regulation of microtubule biogenesis and organization of the tubulin cytoskeleton. Plays a role in the regulation of insulin secretion in response to elevated glucose levels. Part of a ternary complex that contains PAK1, DVL1 and MUSK that is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Phosphorylates MYL9/MLC2. Phosphorylates RAF1 at Ser-338 and Ser-339 resulting in: activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2. Phosphorylates SNAI1 at Ser-246 promoting its transcriptional repressor activity by increasing its accumulation in the nucleus. In podocytes, promotes NR3C2 nuclear localization. Required for atypical chemokine receptor ACKR2-induced phosphorylation of LIMK1 and cofilin (CFL1) and for the up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3, maybe through CFL1 phosphorylation and inactivation.

Enzyme Numbers (IUBMB) for PAK1 Gene

Gene Ontology (GO) - Molecular Function for PAK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity IDA 8805275
GO:0004674 protein serine/threonine kinase activity TAS --
GO:0004702 receptor signaling protein serine/threonine kinase activity IBA --
GO:0004713 protein tyrosine kinase activity --
GO:0005515 contributes_to protein binding IPI 11950598
genes like me logo Genes that share ontologies with PAK1: view
genes like me logo Genes that share phenotypes with PAK1: view

Animal Models for PAK1 Gene

MGI Knock Outs for PAK1:

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for PAK1

In Situ Assay Products

Flow Cytometry Products

No data available for Transcription Factor Targeting and HOMER Transcription for PAK1 Gene

Localization for PAK1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for PAK1 Gene

Cytoplasm. Cell junction, focal adhesion. Cell membrane. Cell projection, ruffle membrane. Note=Recruited to the cell membrane by interaction with CDC42 and RAC1. Recruited to focal adhesions upon activation. Colocalized with CIB1 within membrane ruffles during cell spreading upon readhesion to fibronectin.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for PAK1 Gene COMPARTMENTS Subcellular localization image for PAK1 gene
Compartment Confidence
plasma membrane 5
cytosol 4
golgi apparatus 4
nucleus 4
cytoskeleton 3
endosome 1
extracellular 1

Gene Ontology (GO) - Cellular Components for PAK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001726 ruffle IDA 12912914
GO:0005634 nucleus --
GO:0005737 cytoplasm IDA --
GO:0005794 Golgi apparatus IDA --
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with PAK1: view

Pathways for PAK1 Gene

genes like me logo Genes that share pathways with PAK1: view

PCR Array Products

Gene Ontology (GO) - Biological Process for PAK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000165 NOT MAPK cascade IDA 8805275
GO:0000278 NOT mitotic cell cycle IBA --
GO:0001666 response to hypoxia IEA --
GO:0001934 positive regulation of protein phosphorylation IMP 23633677
GO:0002223 stimulatory C-type lectin receptor signaling pathway TAS --
genes like me logo Genes that share ontologies with PAK1: view

Compounds for PAK1 Gene

(5) Tocris Compounds for PAK1 Gene

Compound Action Cas Number
(5Z)-7-Oxozeaenol Potent and selective TAK1 MAPKKK inhibitor [66018-38-0]
BMS 509744 Potent and selective ITK kinase inhibitor [439575-02-7]
CHR 6494 trifluoroacetate Potent and selective haspin kinase inhibitor [1458630-17-5]
GSK 650394 Serum- and glucocorticoid-regulated kinase (SGK) inhibitor [890842-28-1]
UNC 3230 Potent and selective PIP5K1C inhibitor; antinociceptive [1031602-63-7]

(4) HMDB Compounds for PAK1 Gene

Compound Synonyms Cas Number PubMed IDs
Adenosine triphosphate
  • 5'-(tetrahydrogen triphosphate) Adenosine
56-65-5
ADP
  • adenosindiphosphorsaeure
58-64-0
Guanosine triphosphate
  • 5'-GTP
86-01-1
Magnesium
  • Magnesium
7439-95-4

(30) Novoseek inferred chemical compound relationships for PAK1 Gene

Compound -log(P) Hits PubMed IDs
threonine 69 17
serine 58.8 16
depsipeptide 47.8 5
phosphatidylinositol 46.3 10
ly294002 40.6 3
genes like me logo Genes that share compounds with PAK1: view

Transcripts for PAK1 Gene

Unigene Clusters for PAK1 Gene

P21 protein (Cdc42/Rac)-activated kinase 1:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for PAK1

Primer Products

  • OriGene qSTAR qPCR primer pairs in human,mouse,rat for PAK1

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for PAK1 Gene

No ASD Table

Relevant External Links for PAK1 Gene

GeneLoc Exon Structure for
PAK1
ECgene alternative splicing isoforms for
PAK1

Expression for PAK1 Gene

mRNA expression in normal human tissues for PAK1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for PAK1 Gene

This gene is overexpressed in Brain - Frontal Cortex (BA9) (4.9), Brain - Cortex (4.5), and Whole Blood (4.1).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for PAK1 Gene

SOURCE GeneReport for Unigene cluster for PAK1 Gene Hs.435714

genes like me logo Genes that share expressions with PAK1: view

In Situ Assay Products

No data available for mRNA Expression by UniProt/SwissProt for PAK1 Gene

Orthologs for PAK1 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for PAK1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia PAK1 35
  • 94.1 (n)
  • 93.46 (a)
PAK1 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia PAK1 35
  • 93.32 (n)
  • 99.26 (a)
PAK1 36
  • 95 (a)
OneToOne
dog
(Canis familiaris)
Mammalia PAK1 35
  • 93.69 (n)
  • 96.87 (a)
PAK1 36
  • 93 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Pak1 35
  • 92.34 (n)
  • 99.26 (a)
Pak1 16
Pak1 36
  • 95 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia PAK1 36
  • 93 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia PAK1 36
  • 75 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Pak1 35
  • 92.46 (n)
  • 99.08 (a)
chicken
(Gallus gallus)
Aves PAK1 35
  • 82.81 (n)
  • 94.84 (a)
PAK1 36
  • 91 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia PAK1 36
  • 92 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia Str.7395 35
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.9895 35
zebrafish
(Danio rerio)
Actinopterygii -- 35
pak1 35
  • 76.22 (n)
  • 87.99 (a)
pak1 36
  • 88 (a)
OneToMany
pak1 36
  • 75 (a)
OneToMany
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP002986 35
  • 63.27 (n)
  • 68.85 (a)
fruit fly
(Drosophila melanogaster)
Insecta Pak 35
  • 65.35 (n)
  • 70.95 (a)
Pak 36
  • 42 (a)
OneToMany
Pak 37
  • 82 (a)
Pak3 37
  • 59 (a)
worm
(Caenorhabditis elegans)
Secernentea pak-1 35
  • 58.65 (n)
  • 62.23 (a)
pak-1 36
  • 53 (a)
OneToMany
C45B11.1b 37
  • 38 (a)
Y38F1A.10 37
  • 58 (a)
C45B11.1a 37
  • 38 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes STE20 35
  • 55.53 (n)
  • 55.17 (a)
STE20 38
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes shk1 35
  • 52.41 (n)
  • 53.35 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.3720 36
  • 59 (a)
OneToMany
Species with no ortholog for PAK1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for PAK1 Gene

ENSEMBL:
Gene Tree for PAK1 (if available)
TreeFam:
Gene Tree for PAK1 (if available)

Paralogs for PAK1 Gene

Paralogs for PAK1 Gene

genes like me logo Genes that share paralogs with PAK1: view

Variants for PAK1 Gene

Sequence variations from dbSNP and Humsavar for PAK1 Gene

SNP ID Clin Chr 11 pos Sequence Context AA Info Type MAF
rs51500 -- 77,340,775(-) TATCT(A/G)TATTC intron-variant
rs98703 -- 77,390,839(+) GGAAG(G/T)GAAGG intron-variant
rs471498 -- 77,420,930(-) gtggc(G/T)ctatt intron-variant
rs471974 -- 77,425,247(+) ATTCC(A/C)AAAAC intron-variant
rs472691 -- 77,392,953(+) ACATA(C/G)TGAGT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for PAK1 Gene

Variant ID Type Subtype PubMed ID
esv2744811 CNV Deletion 23290073

Relevant External Links for PAK1 Gene

HapMap Linkage Disequilibrium report
PAK1

PCR Panel Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for PAK1 Gene

Disorders for PAK1 Gene

(1) University of Copenhagen DISEASES for PAK1 Gene

(18) Novoseek inferred disease relationships for PAK1 Gene

Disease -log(P) Hits PubMed IDs
breast cancer 44.6 42
cancer 24.4 23
tumors 23.1 40
neurofibromatosis 22.9 2
ovarian carcinoma 21 7

Relevant External Links for PAK1

Genetic Association Database (GAD)
PAK1
Human Genome Epidemiology (HuGE) Navigator
PAK1
genes like me logo Genes that share disorders with PAK1: view

No data available for UniProtKB/Swiss-Prot for PAK1 Gene

Publications for PAK1 Gene

  1. Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells. (PMID: 9395435) Sells M.A. … Chernoff J. (Curr. Biol. 1997) 3 4 23
  2. Differential effects of PAK1-activating mutations reveal activity- dependent and -independent effects on cytoskeletal regulation. (PMID: 9774440) Frost J.A. … Cobb M.H. (J. Biol. Chem. 1998) 3 4 23
  3. Identification of a central phosphorylation site in p21-activated kinase regulating autoinhibition and kinase activity. (PMID: 10551809) Zenke F.T. … Bokoch G.M. (J. Biol. Chem. 1999) 3 4 23
  4. Pak1 kinase homodimers are autoinhibited in trans and dissociated upon activation by Cdc42 and Rac1. (PMID: 11804587) Parrini M.C. … Mayer B.J. (Mol. Cell 2002) 3 4 23
  5. The C-terminal kinase domain of the p34cdc2-related PITSLRE protein kinase (p110C) associates with p21-activated kinase 1 and inhibits its activity during anoikis. (PMID: 12624090) Chen S. … Gu J. (J. Biol. Chem. 2003) 3 4 23

Products for PAK1 Gene

  • Addgene plasmids for PAK1

Sources for PAK1 Gene

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