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Aliases for MGMT Gene

Aliases for MGMT Gene

  • O-6-Methylguanine-DNA Methyltransferase 2 3
  • 6-O-Methylguanine-DNA Methyltransferase 3 4
  • O-6-Methylguanine-DNA-Alkyltransferase 3 4
  • EC 2.1.1.63 4 63
  • Methylated DNA Protein Cysteine Methyltransferase 3
  • O6-Methylguanine-DNA Methyltransferase 3
  • Methylguanine-DNA Methyltransferase 3

External Ids for MGMT Gene

Previous GeneCards Identifiers for MGMT Gene

  • GC10P130078
  • GC10P130307
  • GC10P131195
  • GC10P130739
  • GC10P130808
  • GC10P131155
  • GC10P124842

Summaries for MGMT Gene

Entrez Gene Summary for MGMT Gene

  • Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015]

GeneCards Summary for MGMT Gene

MGMT (O-6-Methylguanine-DNA Methyltransferase) is a Protein Coding gene. Diseases associated with MGMT include spinal cord astrocytoma and central nervous system lymphoma, primary. Among its related pathways are DNA Damage and Chks in Checkpoint Regulation. GO annotations related to this gene include calcium ion binding and damaged DNA binding.

UniProtKB/Swiss-Prot for MGMT Gene

  • Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated

Tocris Summary for MGMT Gene

  • Histone methyltransferases (HMTs) are a group of enyzmes that catalyze the transfer of methyl groups from S-adenosylmethionine (SAM) to histones. This occurs predominantly on the side chains of lysine and arginine residues in histones H3 and H4.

Gene Wiki entry for MGMT Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MGMT Gene

Genomics for MGMT Gene

Regulatory Elements for MGMT Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for MGMT Gene

Chromosome:
10
Start:
129,467,184 bp from pter
End:
129,768,042 bp from pter
Size:
300,859 bases
Orientation:
Plus strand

Genomic View for MGMT Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
GeneLoc Logo Gene Density

RefSeq DNA sequence for MGMT Gene

Proteins for MGMT Gene

  • Protein details for MGMT Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P16455-MGMT_HUMAN
    Recommended name:
    Methylated-DNA--protein-cysteine methyltransferase
    Protein Accession:
    P16455
    Secondary Accessions:
    • Q5VY78

    Protein attributes for MGMT Gene

    Size:
    207 amino acids
    Molecular mass:
    21646 Da
    Cofactor:
    Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion.;
    Quaternary structure:
    No Data Available
    Miscellaneous:
    • This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles

    Three dimensional structures from OCA and Proteopedia for MGMT Gene

neXtProt entry for MGMT Gene

Proteomics data for MGMT Gene at MOPED

Selected DME Specific Peptides for MGMT Gene

Post-translational modifications for MGMT Gene

  • Ubiquitination at Lys 125 and Lys 178
  • Modification sites at PhosphoSitePlus

Other Protein References for MGMT Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for MGMT (MGMT)

Domains & Families for MGMT Gene

Suggested Antigen Peptide Sequences for MGMT Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

P16455

UniProtKB/Swiss-Prot:

MGMT_HUMAN :
  • Belongs to the MGMT family.
Family:
  • Belongs to the MGMT family.
genes like me logo Genes that share domains with MGMT: view

No data available for Gene Families for MGMT Gene

Function for MGMT Gene

Molecular function for MGMT Gene

GENATLAS Biochemistry:
O6-methylguanine-DNA-methyltransferase
UniProtKB/Swiss-Prot CatalyticActivity:
DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.
UniProtKB/Swiss-Prot Function:
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated

Enzyme Numbers (IUBMB) for MGMT Gene

Gene Ontology (GO) - Molecular Function for MGMT Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003677 DNA binding TAS 2188979
GO:0003684 damaged DNA binding IBA --
GO:0003824 catalytic activity --
GO:0003908 methylated-DNA-[protein]-cysteine S-methyltransferase activity IBA --
GO:0005509 calcium ion binding IEA --
genes like me logo Genes that share ontologies with MGMT: view
genes like me logo Genes that share phenotypes with MGMT: view

Animal Models for MGMT Gene

MGI Knock Outs for MGMT:

Animal Model Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for MGMT

In Situ Assay Products

Flow Cytometry Products

No data available for Transcription Factor Targets and HOMER Transcription for MGMT Gene

Localization for MGMT Gene

Subcellular locations from UniProtKB/Swiss-Prot for MGMT Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for MGMT Gene COMPARTMENTS Subcellular localization image for MGMT gene
Compartment Confidence
nucleus 5
cytoskeleton 1
cytosol 1
mitochondrion 1

Gene Ontology (GO) - Cellular Components for MGMT Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus TAS 2188979
GO:0005654 nucleoplasm TAS --
GO:0016020 membrane IDA 19946888
genes like me logo Genes that share ontologies with MGMT: view

Pathways & Interactions for MGMT Gene

genes like me logo Genes that share pathways with MGMT: view

Pathways by source for MGMT Gene

2 Qiagen pathways for MGMT Gene
2 Cell Signaling Technology pathways for MGMT Gene

PCR Array Products

Interacting Proteins for MGMT Gene

Gene Ontology (GO) - Biological Process for MGMT Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006266 DNA ligation TAS 2188979
GO:0006281 DNA repair TAS --
GO:0006306 DNA methylation TAS 2405387
GO:0006307 DNA dealkylation involved in DNA repair TAS --
GO:0006974 cellular response to DNA damage stimulus --
genes like me logo Genes that share ontologies with MGMT: view

No data available for SIGNOR curated interactions for MGMT Gene

Drugs & Compounds for MGMT Gene

(54) Drugs for MGMT Gene - From: NovoSeek, DGIdb, ApexBio, Tocris, HMDB, DrugBank, and ClinicalTrials

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
L-Cysteine Approved Nutra Target 0
Dacarbazine Approved, Investigational Pharma 389
Lomustine Approved Pharma Antineoplastic drug 71
Procarbazine Approved Pharma 67
Temozolomide Approved, Investigational Pharma 628

(54) Additional Compounds for MGMT Gene - From: NovoSeek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
12-O-Tetradecanoylphorbol 13-acetate
5-Aza-2deoxycytidine
5fluorouracil
5-methylcytosine
  • 4-Amino-5-methyl-2-(1H)-Pyrimidinone
  • 4-Amino-5-methyl-2-pyrimidinol
  • 5-Methyl-Cytosine
  • 5-Methylcytosine
  • 5-Methylcytosine>96
554-01-8
7,12-dimethylbenz(a)anthracene
genes like me logo Genes that share compounds with MGMT: view

Transcripts for MGMT Gene

Unigene Clusters for MGMT Gene

O-6-methylguanine-DNA methyltransferase:
Representative Sequences:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for MGMT

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for MGMT Gene

No ASD Table

Relevant External Links for MGMT Gene

GeneLoc Exon Structure for
MGMT
ECgene alternative splicing isoforms for
MGMT

Expression for MGMT Gene

mRNA expression in normal human tissues for MGMT Gene

mRNA differential expression in normal tissues according to GTEx for MGMT Gene

This gene is overexpressed in Liver (x5.5).

Protein differential expression in normal tissues from HIPED for MGMT Gene

This gene is overexpressed in Lymph node (13.8).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for MGMT Gene



SOURCE GeneReport for Unigene cluster for MGMT Gene Hs.501522

genes like me logo Genes that share expression patterns with MGMT: view

Protein tissue co-expression partners for MGMT Gene

- Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and mRNA Expression by UniProt/SwissProt for MGMT Gene

Orthologs for MGMT Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for MGMT Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia MGMT 35
  • 77.73 (n)
  • 68.72 (a)
dog
(Canis familiaris)
Mammalia MGMT 35
  • 75.36 (n)
  • 64.73 (a)
MGMT 36
  • 61 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Mgmt 35
  • 75.57 (n)
  • 70.39 (a)
Mgmt 16
Mgmt 36
  • 69 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia MGMT 35
  • 98.88 (n)
  • 97.9 (a)
MGMT 36
  • 98 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Mgmt 35
  • 74.02 (n)
  • 70.1 (a)
platypus
(Ornithorhynchus anatinus)
Mammalia MGMT 36
  • 55 (a)
OneToOne
chicken
(Gallus gallus)
Aves MGMT 35
  • 63.8 (n)
  • 59.68 (a)
MGMT 36
  • 41 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia MGMT 36
  • 69 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia mgmt 35
  • 60.75 (n)
  • 56.21 (a)
Str.8455 35
zebrafish
(Danio rerio)
Actinopterygii mgmt 35
  • 51.9 (n)
  • 48.5 (a)
mgmt 36
  • 42 (a)
OneToOne
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes MGT1 38
Species with no ortholog for MGMT:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • oppossum (Monodelphis domestica)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for MGMT Gene

ENSEMBL:
Gene Tree for MGMT (if available)
TreeFam:
Gene Tree for MGMT (if available)

Paralogs for MGMT Gene

No data available for Paralogs for MGMT Gene

Variants for MGMT Gene

Sequence variations from dbSNP and Humsavar for MGMT Gene

SNP ID Clin Chr 10 pos Sequence Context AA Info Type MAF
rs12917 -, - 129,708,019(+) CGGCT(C/T)TTCAC reference, missense
rs471357 -- 129,591,109(+) GGGGG(C/G)GCAGC intron-variant
rs471390 -- 129,584,561(-) ttaca(C/T)tgtga intron-variant
rs473073 -- 129,631,382(-) AGAGT(A/C)CTTCA intron-variant
rs473771 -- 129,609,891(+) AGAAA(A/C)AGGCC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for MGMT Gene

Variant ID Type Subtype PubMed ID
dgv174e199 CNV Deletion 23128226
nsv7617 CNV Insertion 18451855
esv21706 CNV Loss 19812545
nsv513300 CNV Insertion 21212237
esv2673495 CNV Deletion 23128226
nsv24217 CNV Insertion 16902084
esv33589 CNV Loss 17666407
nsv516662 CNV Loss 19592680
esv2743445 CNV Deletion 23290073
esv2673844 CNV Deletion 23128226
nsv896101 CNV Loss 21882294
nsv896102 CNV Loss 21882294
esv26422 CNV Gain 19812545

Variation tolerance for MGMT Gene

Residual Variation Intolerance Score: 57.95% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 6.20; 76.01% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for MGMT Gene

HapMap Linkage Disequilibrium report
MGMT
Human Gene Mutation Database (HGMD)
MGMT

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for MGMT Gene

Disorders for MGMT Gene

MalaCards: The human disease database

(38) MalaCards diseases for MGMT Gene - From: Orphanet, DISEASES, NovoSeek, and GeneCards

Disorder Aliases PubMed IDs
spinal cord astrocytoma
  • spinal astrocytoma
central nervous system lymphoma, primary
  • primary cerebral lymphoma
malignant glioma
  • glioma
central nervous system lymphoma
  • microglioma
brain cancer
  • tumor of the brain
- elite association
Search MGMT in MalaCards View complete list of genes associated with diseases

Relevant External Links for MGMT

Genetic Association Database (GAD)
MGMT
Human Genome Epidemiology (HuGE) Navigator
MGMT
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
MGMT
genes like me logo Genes that share disorders with MGMT: view

No data available for UniProtKB/Swiss-Prot and Genatlas for MGMT Gene

Publications for MGMT Gene

  1. Increased expression of the MGMT repair protein mediated by cysteine prodrugs and chemopreventative natural products in human lymphocytes and tumor cell lines. (PMID: 16950796) Niture S.K. … Srivenugopal K.S. (Carcinogenesis 2007) 23 25 26 67
  2. O(6)-Methylguanine-DNA methyltransferase expression and prognostic value in brain metastases of lung cancers. (PMID: 19740564) Wu P.F. … Lin C.H. (Lung Cancer 2010) 23 67
  3. High incidence of MGMT and RARbeta promoter methylation in primary glioblastomas: association with histopathological characteristics, inflammatory mediators and clinical outcome. (PMID: 19809523) Piperi C. … Papavassiliou A.G. (Mol. Med. 2010) 23 67
  4. Recurrent glioblastoma of childhood treated with bevacizumab: case report and molecular features. (PMID: 19823845) Zhang W. … Jiang T. (Childs Nerv Syst 2010) 23 67
  5. Role of histone modifications and DNA methylation in the regulation of O6-methylguanine-DNA methyltransferase gene expression in human stomach cancer cells. (PMID: 19857042) Meng C.F. … Dai D.Q. (Cancer Invest. 2010) 23 67

Products for MGMT Gene

Sources for MGMT Gene

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