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Aliases for MGMT Gene

Aliases for MGMT Gene

  • O-6-Methylguanine-DNA Methyltransferase 2 3
  • 6-O-Methylguanine-DNA Methyltransferase 3 4
  • O-6-Methylguanine-DNA-Alkyltransferase 3 4
  • EC 2.1.1.63 4 64
  • Methylated-DNA--Protein-Cysteine Methyltransferase 3
  • O6-Methylguanine-DNA Methyltransferase 3
  • Methylguanine-DNA Methyltransferase 3

External Ids for MGMT Gene

Summaries for MGMT Gene

GeneCards Summary for MGMT Gene

MGMT (O-6-Methylguanine-DNA Methyltransferase) is a Protein Coding gene. Diseases associated with MGMT include spinal cord astrocytoma and astrocytoma. Among its related pathways are Cell Cycle / Checkpoint Control and Chks in Checkpoint Regulation. GO annotations related to this gene include calcium ion binding and DNA-methyltransferase activity.

UniProtKB/Swiss-Prot for MGMT Gene

  • Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated

Tocris Summary for MGMT Gene

  • MGMT encodes O-6-methylguanine-DNA methyltransferase, an enzyme involved in DNA repair. This enzyme removes alkylating lesions at the O6 of guanine residues. This can undermine cancer therapy using alkylating agents, and thus inhibitors of MGMT activity are of interest in cancer research.

Gene Wiki entry for MGMT Gene

No data available for Entrez Gene Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for MGMT Gene

Genomics for MGMT Gene

Genomic Location for MGMT Gene

Start:
129,467,184 bp from pter
End:
129,768,007 bp from pter
Size:
300,824 bases
Orientation:
Plus strand

Genomic View for MGMT Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for MGMT Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for MGMT Gene

Regulatory Elements for MGMT Gene

Proteins for MGMT Gene

  • Protein details for MGMT Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P16455-MGMT_HUMAN
    Recommended name:
    Methylated-DNA--protein-cysteine methyltransferase
    Protein Accession:
    P16455
    Secondary Accessions:
    • Q5VY78

    Protein attributes for MGMT Gene

    Size:
    207 amino acids
    Molecular mass:
    21646 Da
    Cofactor:
    Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion.;
    Quaternary structure:
    No Data Available
    Miscellaneous:
    • This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles

    Three dimensional structures from OCA and Proteopedia for MGMT Gene

neXtProt entry for MGMT Gene

Proteomics data for MGMT Gene at MOPED

Selected DME Specific Peptides for MGMT Gene

Post-translational modifications for MGMT Gene

  • Modification sites at PhosphoSitePlus
  • Ubiquitination at Lys125 and Lys178

Other Protein References for MGMT Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

Domains for MGMT Gene

UniProtKB/Swiss-Prot:

MGMT_HUMAN
Family:
  • Belongs to the MGMT family.:
    • P16455
genes like me logo Genes that share domains with MGMT: view

No data available for Gene Families for MGMT Gene

Function for MGMT Gene

Molecular function for MGMT Gene

GENATLAS Biochemistry: O6-methylguanine-DNA-methyltransferase
UniProtKB/Swiss-Prot CatalyticActivity: DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.
UniProtKB/Swiss-Prot Function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated

Enzyme Numbers (IUBMB) for MGMT Gene

Gene Ontology (GO) - Molecular Function for MGMT Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003677 DNA binding TAS 2188979
GO:0003684 damaged DNA binding IBA --
GO:0003824 catalytic activity --
GO:0003908 methylated-DNA-[protein]-cysteine S-methyltransferase activity IBA --
GO:0005509 calcium ion binding IEA --
genes like me logo Genes that share ontologies with MGMT: view
genes like me logo Genes that share phenotypes with MGMT: view

Animal Models for MGMT Gene

MGI Knock Outs for MGMT:

miRNA for MGMT Gene

miRTarBase miRNAs that target MGMT

No data available for Transcription Factor Targeting and HOMER Transcription for MGMT Gene

Localization for MGMT Gene

Subcellular locations from UniProtKB/Swiss-Prot for MGMT Gene

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for MGMT Gene COMPARTMENTS Subcellular localization image for MGMT gene
Compartment Confidence
nucleus 5
extracellular 2
cytoskeleton 1
cytosol 1
mitochondrion 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for MGMT Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus TAS 2188979
GO:0005654 nucleoplasm TAS --
GO:0016020 membrane IDA 19946888
genes like me logo Genes that share ontologies with MGMT: view

Pathways for MGMT Gene

genes like me logo Genes that share pathways with MGMT: view

Pathways by source for MGMT Gene

2 Qiagen pathways for MGMT Gene
3 Cell Signaling Technology pathways for MGMT Gene
2 Reactome pathways for MGMT Gene

Interacting Proteins for MGMT Gene

Gene Ontology (GO) - Biological Process for MGMT Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006266 DNA ligation TAS 2188979
GO:0006281 DNA repair TAS --
GO:0006306 DNA methylation TAS 2405387
GO:0006307 DNA dealkylation involved in DNA repair TAS --
GO:0006974 cellular response to DNA damage stimulus --
genes like me logo Genes that share ontologies with MGMT: view

Compounds for MGMT Gene

(5) Tocris Compounds for MGMT Gene

Compound Action Cas Number
3-Deazaneplanocin A hydrochloride Histone methyltransferase inhibitor [120964-45-6]
BIX 01294 G9a-like protein and G9a histone lysine methyltransferase inhibitor [935693-62-2]
SGC 0946 Highly potent and selective DOT1L inhibitor; cell permeable
UNC 0638 Selective G9a and GLP histone lysine methyltransferase inhibitor [1255517-77-1]
UNC 0646 Potent and selective G9a/GLP inhibitor [1320288-17-2]

(1) HMDB Compounds for MGMT Gene

Compound Synonyms Cas Number PubMed IDs
L-Cysteine
  • (+)-2-Amino-3-mercaptopropionic acid
52-90-4

(3) Drugbank Compounds for MGMT Gene

Compound Synonyms Cas Number Type Actions PubMed IDs
Benzylcysteine
target
L-Cysteine
  • (R)-2-Amino-3-mercaptopropanoic acid
52-90-4 target
S-Methylcysteine
1187-84-4 target

(99) Novoseek inferred chemical compound relationships for MGMT Gene

Compound -log(P) Hits PubMed IDs
o6-methylguanine 97.2 170
temozolomide 95.5 269
o6-benzylguanine 94.3 181
chloroethylnitrosourea 89.2 46
chloroethyl nitrosoureas 88.9 25
genes like me logo Genes that share compounds with MGMT: view

Transcripts for MGMT Gene

Unigene Clusters for MGMT Gene

O-6-methylguanine-DNA methyltransferase:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for MGMT Gene

No ASD Table

Relevant External Links for MGMT Gene

GeneLoc Exon Structure for
MGMT
ECgene alternative splicing isoforms for
MGMT

Expression for MGMT Gene

mRNA expression in normal human tissues for MGMT Gene

mRNA differential expression in normal tissues according to GTEx for MGMT Gene

This gene is overexpressed in Liver (5.5).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, and MOPED for MGMT Gene

SOURCE GeneReport for Unigene cluster for MGMT Gene Hs.501522

genes like me logo Genes that share expressions with MGMT: view

Orthologs for MGMT Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for MGMT Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia MGMT 36
  • 98.88 (n)
  • 97.9 (a)
MGMT 37
  • 98 (a)
OneToOne
cow
(Bos Taurus)
Mammalia MGMT 36
  • 77.73 (n)
  • 68.72 (a)
dog
(Canis familiaris)
Mammalia MGMT 36
  • 75.36 (n)
  • 64.73 (a)
MGMT 37
  • 61 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Mgmt 36
  • 75.57 (n)
  • 70.39 (a)
Mgmt 16
Mgmt 37
  • 69 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia MGMT 37
  • 55 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Mgmt 36
  • 74.02 (n)
  • 70.1 (a)
chicken
(Gallus gallus)
Aves MGMT 36
  • 63.8 (n)
  • 59.68 (a)
MGMT 37
  • 41 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia MGMT 37
  • 69 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia mgmt 36
  • 60.75 (n)
  • 56.21 (a)
Str.8455 36
zebrafish
(Danio rerio)
Actinopterygii mgmt 36
  • 51.9 (n)
  • 48.5 (a)
mgmt 37
  • 42 (a)
OneToOne
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes MGT1 39
Species with no ortholog for MGMT:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • oppossum (Monodelphis domestica)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for MGMT Gene

ENSEMBL:
Gene Tree for MGMT (if available)
TreeFam:
Gene Tree for MGMT (if available)

Paralogs for MGMT Gene

No data available for Paralogs for MGMT Gene

Variants for MGMT Gene

Sequence variations from dbSNP and Humsavar for MGMT Gene

SNP ID Clin Chr 10 pos Sequence Context AA Info Type MAF
rs12917 -, - 129,708,019(+) CGGCT(C/T)TTCAC reference, missense
rs471357 -- 129,591,109(+) GGGGG(C/G)GCAGC intron-variant
rs471390 -- 129,584,561(-) ttaca(C/T)tgtga intron-variant
rs473073 -- 129,631,382(-) AGAGT(A/C)CTTCA intron-variant
rs473771 -- 129,609,891(+) AGAAA(A/C)AGGCC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for MGMT Gene

Variant ID Type Subtype PubMed ID
dgv174e199 CNV Deletion 23128226
nsv7617 CNV Insertion 18451855
esv21706 CNV Loss 19812545
nsv513300 CNV Insertion 21212237
esv2673495 CNV Deletion 23128226
nsv24217 CNV Insertion 16902084
esv33589 CNV Loss 17666407
nsv516662 CNV Loss 19592680
esv2743445 CNV Deletion 23290073
esv2673844 CNV Deletion 23128226
nsv896101 CNV Loss 21882294
nsv896102 CNV Loss 21882294
esv26422 CNV Gain 19812545

Relevant External Links for MGMT Gene

HapMap Linkage Disequilibrium report
MGMT
Human Gene Mutation Database (HGMD)
MGMT

Disorders for MGMT Gene

(1) University of Copenhagen DISEASES for MGMT Gene

(93) Novoseek inferred disease relationships for MGMT Gene

Disease -log(P) Hits PubMed IDs
glioblastoma 87.5 173
glioma 80.8 183
glioblastoma multiforme 77.8 29
malignant glioma 75.5 33
tumors 75.3 320

Relevant External Links for MGMT

Genetic Association Database (GAD)
MGMT
Human Genome Epidemiology (HuGE) Navigator
MGMT
genes like me logo Genes that share disorders with MGMT: view

No data available for UniProtKB/Swiss-Prot for MGMT Gene

Publications for MGMT Gene

  1. Isolation and structural characterization of a cDNA clone encoding the human DNA repair protein for O6-alkylguanine. (PMID: 2405387) Tano K. … Mitra S. (Proc. Natl. Acad. Sci. U.S.A. 1990) 3 4 23
  2. Structural and immunological comparison of indigenous human O6- methylguanine-DNA methyltransferase with that encoded by a cloned cDNA. (PMID: 1985934) von Wronski M.A. … Brent T.P. (J. Biol. Chem. 1991) 3 4 23
  3. cDNA cloning and chromosomal assignment of the human O6- methylguanine-DNA methyltransferase. cDNA expression in Escherichia coli and gene expression in human cells. (PMID: 2188979) Rydberg B. … Karran P. (J. Biol. Chem. 1990) 3 4 23
  4. Specificities of human, rat and E. coli O6-methylguanine-DNA methyltransferases towards the repair of O6-methyl and O6-ethylguanine in DNA. (PMID: 8202360) Liem L.-K. … Li B.F.L. (Nucleic Acids Res. 1994) 3 4 23
  5. Novel O6-methylguanine-DNA methyltransferase SNPs: a frequency comparison of patients with familial melanoma and healthy individuals in Sweden. (PMID: 12402349) EgyhA!zi S. … Ringborg U. (Hum. Mutat. 2002) 3 23 49

Products for MGMT Gene

Sources for MGMT Gene

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