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Aliases for LIMK1 Gene

Aliases for LIMK1 Gene

  • LIM Domain Kinase 1 2 3
  • EC 2.7.11.1 4 63
  • LIMK-1 3 4
  • LIMK 3 4
  • LIM Motif-Containing Protein Kinase 3

External Ids for LIMK1 Gene

Previous GeneCards Identifiers for LIMK1 Gene

  • GC07P072138
  • GC07P072896
  • GC07P072910
  • GC07P072942
  • GC07P073136
  • GC07P073497
  • GC07P069382

Summaries for LIMK1 Gene

Entrez Gene Summary for LIMK1 Gene

  • There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Feb 2011]

GeneCards Summary for LIMK1 Gene

LIMK1 (LIM Domain Kinase 1) is a Protein Coding gene. Diseases associated with LIMK1 include intracranial aneurysm and williams-beuren syndrome. Among its related pathways are Regulation of actin cytoskeleton and Regulation of actin cytoskeleton. GO annotations related to this gene include protein heterodimerization activity and protein kinase activity. An important paralog of this gene is LIMK2.

UniProtKB/Swiss-Prot for LIMK1 Gene

  • Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1).

Tocris Summary for LIMK1 Gene

  • LIM kinases (LIMKs), EC 2.7.11.1, are dual specificity kinases (serine/threonine and tyrosine), which phosphorylate and inactivate cofilin (a key regulator of actin cytoskeleton dynamics). There are two isoforms of LIMK: LIMK1 and LIMK2.

Gene Wiki entry for LIMK1 Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for LIMK1 Gene

Genomics for LIMK1 Gene

Regulatory Elements for LIMK1 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for LIMK1 Gene

Start:
74,082,933 bp from pter
End:
74,122,525 bp from pter
Size:
39,593 bases
Orientation:
Plus strand

Genomic View for LIMK1 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for LIMK1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for LIMK1 Gene

Proteins for LIMK1 Gene

  • Protein details for LIMK1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P53667-LIMK1_HUMAN
    Recommended name:
    LIM domain kinase 1
    Protein Accession:
    P53667
    Secondary Accessions:
    • B7Z6I8
    • D3DXF4
    • D3DXF5
    • O15283
    • Q15820
    • Q15821
    • Q75MU3
    • Q9Y5Q1

    Protein attributes for LIMK1 Gene

    Size:
    647 amino acids
    Molecular mass:
    72585 Da
    Quaternary structure:
    • Interacts (via LIM domain) with the cytoplasmic domain of NRG1 (By similarity). Interacts with NISCH (By similarity). Interacts with RLIM and RNF6 (By similarity). Self-associates to form homodimers. Interacts with HSP90AA1; this interaction promotes LIMK1 dimerization and subsequent transphosphorylation. Interacts with CDN1C. Interacts with SSH1. Interacts with ROCK1.

    Three dimensional structures from OCA and Proteopedia for LIMK1 Gene

    Alternative splice isoforms for LIMK1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for LIMK1 Gene

Proteomics data for LIMK1 Gene at MOPED

Post-translational modifications for LIMK1 Gene

  • Autophosphorylated (By similarity). Phosphorylated on Thr-508 by ROCK1 and PAK1, resulting in activation. Phosphorylated by PAK4 which increases the ability of LIMK1 to phosphorylate cofilin. Phosphorylated at Ser-323 by MAPKAPK2 during activation of VEGFA-induced signaling, which results in activation of LIMK1 and promotion of actin reorganization, cell migration, and tubule formation of endothelial cells. Dephosphorylated and inactivated by SSH1. Phosphorylated by CDC42BP.
  • Ubiquitinated. Lys-48-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM (By similarity).
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for LIMK1 Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for LIMK1 (LIMK1)

Domains for LIMK1 Gene

Gene Families for LIMK1 Gene

Suggested Antigen Peptide Sequences for LIMK1 Gene

Graphical View of Domain Structure for InterPro Entry

P53667

UniProtKB/Swiss-Prot:

LIMK1_HUMAN :
  • P53667
Domain:
  • Contains 2 LIM zinc-binding domains.
  • Contains 1 PDZ (DHR) domain.
  • Contains 1 protein kinase domain.
Family:
  • Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
genes like me logo Genes that share domains with LIMK1: view

Function for LIMK1 Gene

Molecular function for LIMK1 Gene

GENATLAS Biochemistry: LIM motif containing protein kinase 1,serine only protein kinase,cytosolic,highly expressed in neurons,regulating actin dynamics through phosphorylation of cofilin,which leads to accumulation of F-actin,deleted in Williams syndrome,claimed to be responsible for the cognitive defect of the syndrome,but contradictory evidence,see WBS1
UniProtKB/Swiss-Prot CatalyticActivity: ATP + a protein = ADP + a phosphoprotein
UniProtKB/Swiss-Prot Function: Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1).

Enzyme Numbers (IUBMB) for LIMK1 Gene

Gene Ontology (GO) - Molecular Function for LIMK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity NAS 8537403
GO:0004674 protein serine/threonine kinase activity TAS --
GO:0004713 protein tyrosine kinase activity --
GO:0005515 protein binding IPI 10559936
GO:0005524 ATP binding IEA --
genes like me logo Genes that share ontologies with LIMK1: view

Phenotypes for LIMK1 Gene

genes like me logo Genes that share phenotypes with LIMK1: view

Animal Models for LIMK1 Gene

MGI Knock Outs for LIMK1:

Animal Model Products

CRISPR Products

miRNA for LIMK1 Gene

miRTarBase miRNAs that target LIMK1

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for LIMK1

In Situ Assay Products

Flow Cytometry Products

No data available for Transcription Factor Targeting and HOMER Transcription for LIMK1 Gene

Localization for LIMK1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for LIMK1 Gene

Cytoplasm. Nucleus. Note=Predominantly found in the cytoplasm.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for LIMK1 Gene COMPARTMENTS Subcellular localization image for LIMK1 gene
Compartment Confidence
nucleus 5
cytosol 4
cytoskeleton 3

Gene Ontology (GO) - Cellular Components for LIMK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus --
GO:0005654 nucleoplasm IDA --
GO:0005737 cytoplasm IDA --
GO:0005829 cytosol TAS --
GO:0005925 focal adhesion IEA --
genes like me logo Genes that share ontologies with LIMK1: view

Pathways for LIMK1 Gene

genes like me logo Genes that share pathways with LIMK1: view

PCR Array Products

Gene Ontology (GO) - Biological Process for LIMK1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006468 protein phosphorylation IMP 23633677
GO:0007165 signal transduction TAS 10428028
GO:0007264 small GTPase mediated signal transduction TAS --
GO:0007266 Rho protein signal transduction TAS 10436159
GO:0007399 nervous system development TAS 8689688
genes like me logo Genes that share ontologies with LIMK1: view

Compounds for LIMK1 Gene

(1) Tocris Compounds for LIMK1 Gene

Compound Action Cas Number
LIMKi 3 Potent LIM kinase inhibitor; antitumor [1338247-35-0]

(2) HMDB Compounds for LIMK1 Gene

Compound Synonyms Cas Number PubMed IDs
Adenosine triphosphate
  • 5'-(tetrahydrogen triphosphate) Adenosine
56-65-5
ADP
  • adenosindiphosphorsaeure
58-64-0

(6) Novoseek inferred chemical compound relationships for LIMK1 Gene

Compound -log(P) Hits PubMed IDs
leptomycin b 62.1 1
threonine 60.7 17
serine 50 19
nocodazole 27.5 1
tyrosine 5.62 4
genes like me logo Genes that share compounds with LIMK1: view

Transcripts for LIMK1 Gene

Unigene Clusters for LIMK1 Gene

LIM domain kinase 1:
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for LIMK1

Primer Products

  • OriGene qSTAR qPCR primer pairs in human,mouse,rat for LIMK1

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for LIMK1 Gene

ExUns: 1 ^ 2a · 2b ^ 3 ^ 4 ^ 5a · 5b · 5c ^ 6 ^ 7a · 7b ^ 8 ^ 9a · 9b ^ 10 ^ 11a · 11b · 11c ^ 12 ^ 13a · 13b ^ 14 ^ 15 ^ 16a · 16b ^ 17a ·
SP1: - - - - - - -
SP2: - - - - - - - -
SP3:
SP4: - - -
SP5: - - - - - -
SP6: -
SP7:
SP8: -
SP9:

ExUns: 17b ^ 18a · 18b ^ 19a · 19b · 19c
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:

Relevant External Links for LIMK1 Gene

GeneLoc Exon Structure for
LIMK1
ECgene alternative splicing isoforms for
LIMK1

Expression for LIMK1 Gene

mRNA expression in normal human tissues for LIMK1 Gene

mRNA differential expression in normal tissues according to GTEx for LIMK1 Gene

This gene is overexpressed in Brain - Cortex (4.2).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for LIMK1 Gene

SOURCE GeneReport for Unigene cluster for LIMK1 Gene Hs.647035

mRNA Expression by UniProt/SwissProt for LIMK1 Gene

P53667-LIMK1_HUMAN
Tissue specificity: Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle
genes like me logo Genes that share expressions with LIMK1: view

In Situ Assay Products

Orthologs for LIMK1 Gene

This gene was present in the common ancestor of animals.

Orthologs for LIMK1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia LIMK1 35
  • 99.64 (n)
  • 100 (a)
LIMK1 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia LIMK1 35
  • 92.12 (n)
  • 97.68 (a)
LIMK1 36
  • 98 (a)
OneToOne
dog
(Canis familiaris)
Mammalia LIMK1 35
  • 90.01 (n)
  • 94.13 (a)
LIMK1 36
  • 94 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Limk1 35
  • 88 (n)
  • 95.21 (a)
Limk1 16
Limk1 36
  • 95 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia LIMK1 36
  • 90 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia LIMK1 36
  • 78 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Limk1 35
  • 88 (n)
  • 95.36 (a)
chicken
(Gallus gallus)
Aves LIMK1 35
  • 82.04 (n)
  • 84.52 (a)
LIMK1 36
  • 82 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia LIMK1 36
  • 83 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia xlimk1 35
tropical clawed frog
(Silurana tropicalis)
Amphibia limk1 35
  • 69.65 (n)
  • 77.04 (a)
zebrafish
(Danio rerio)
Actinopterygii limk1a 35
  • 71.62 (n)
  • 73.87 (a)
limk1a 36
  • 73 (a)
OneToMany
limk1b 36
  • 56 (a)
OneToMany
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP011710 35
  • 32.92 (n)
  • 15.85 (a)
fruit fly
(Drosophila melanogaster)
Insecta LIMK1 35
  • 56.67 (n)
  • 50.74 (a)
LIMK1 36
  • 23 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea K09B11.5 36
  • 19 (a)
ManyToMany
kin-21 36
  • 19 (a)
ManyToMany
T25B9.5 36
  • 17 (a)
ManyToMany
Y52D5A.2 36
  • 18 (a)
ManyToMany
Y69E1A.3 36
  • 17 (a)
ManyToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 41 (a)
OneToMany
Species with no ortholog for LIMK1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for LIMK1 Gene

ENSEMBL:
Gene Tree for LIMK1 (if available)
TreeFam:
Gene Tree for LIMK1 (if available)

Paralogs for LIMK1 Gene

Paralogs for LIMK1 Gene

Selected SIMAP similar genes for LIMK1 Gene using alignment to 4 proteins:

Pseudogenes.org Pseudogenes for LIMK1 Gene

genes like me logo Genes that share paralogs with LIMK1: view

Variants for LIMK1 Gene

Sequence variations from dbSNP and Humsavar for LIMK1 Gene

SNP ID Clin Chr 07 pos Sequence Context AA Info Type MAF
rs149618 -- 74,115,716(-) CAACC(C/T)GTAGA intron-variant
rs150861 -- 74,110,571(-) ggaca(A/G)taaga intron-variant
rs150862 -- 74,109,877(-) ACAGG(A/G)CTCTG intron-variant
rs150863 -- 74,109,839(-) tgaga(A/C)ggaat intron-variant
rs150864 -- 74,109,811(-) tagag(C/T)gcagt intron-variant

Structural Variations from Database of Genomic Variants (DGV) for LIMK1 Gene

Variant ID Type Subtype PubMed ID
nsv888374 CNV Loss 21882294
nsv888375 CNV Loss 21882294
esv1010108 CNV Deletion 20482838
esv1038833 CNV Deletion 17803354
nsv366821 CNV Loss 16902084
nsv464563 CNV Gain 19166990
nsv831030 CNV Gain 17160897

Relevant External Links for LIMK1 Gene

HapMap Linkage Disequilibrium report
LIMK1
Human Gene Mutation Database (HGMD)
LIMK1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for LIMK1 Gene

Disorders for LIMK1 Gene

MalaCards: The human disease database

MalaCards: The human disease database. (2) Diseases for LIMK1 Gene including...

Search for LIMK1 Gene in MalaCards »

UniProtKB/Swiss-Prot

LIMK1_HUMAN
  • Note=LIMK1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.

(2) University of Copenhagen DISEASES for LIMK1 Gene

(12) Novoseek inferred disease relationships for LIMK1 Gene

Disease -log(P) Hits PubMed IDs
williams syndrome 86.1 10
aortic stenosis supravalvular 63.4 2
cognitive deficit 28.1 1
prostate cancer 23.3 6
intracranial aneurysm 19.5 1

Relevant External Links for LIMK1

Genetic Association Database (GAD)
LIMK1
Human Genome Epidemiology (HuGE) Navigator
LIMK1
genes like me logo Genes that share disorders with LIMK1: view

Publications for LIMK1 Gene

  1. Identification of genes from a 500-kb region at 7q11.23 that is commonly deleted in Williams syndrome patients. (PMID: 8812460) Osborne L.R. … Tsui L.-C. (Genomics 1996) 2 3 4 23
  2. Activation of LIM kinases by myotonic dystrophy kinase-related Cdc42- binding kinase alpha. (PMID: 11340065) Sumi T. … Nakamura T. (J. Biol. Chem. 2001) 3 4 23
  3. Cytoskeletal changes regulated by the PAK4 serine/threonine kinase are mediated by LIM kinase 1 and cofilin. (PMID: 11413130) Dan C. … Minden A. (J. Biol. Chem. 2001) 3 4 23
  4. Identification of a human cDNA encoding a novel protein kinase with two repeats of the LIM/double zinc finger motif. (PMID: 8183554) Mizuno K. … Nakamura T. (Oncogene 1994) 3 4 23
  5. Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop. (PMID: 10652353) Ohashi K. … Mizuno K. (J. Biol. Chem. 2000) 3 4 23

Products for LIMK1 Gene

  • Addgene plasmids for LIMK1

Sources for LIMK1 Gene

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