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Aliases for IRF3 Gene

Aliases for IRF3 Gene

  • Interferon Regulatory Factor 3 2 3
  • IRF-3 4

External Ids for IRF3 Gene

Previous GeneCards Identifiers for IRF3 Gene

  • GC19M050821
  • GC19M050530
  • GC19M054838
  • GC19M054854
  • GC19M050162
  • GC19M046539

Summaries for IRF3 Gene

Entrez Gene Summary for IRF3 Gene

  • This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

GeneCards Summary for IRF3 Gene

IRF3 (Interferon Regulatory Factor 3) is a Protein Coding gene. Diseases associated with IRF3 include lymphocytic choriomeningitis and herpes simplex. Among its related pathways are Hemostasis and Epstein-Barr virus infection. GO annotations related to this gene include sequence-specific DNA binding transcription factor activity and identical protein binding. An important paralog of this gene is IRF7.

UniProtKB/Swiss-Prot for IRF3 Gene

  • Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages

Gene Wiki entry for IRF3 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for IRF3 Gene

Genomics for IRF3 Gene

Regulatory Elements for IRF3 Gene

Genomic Location for IRF3 Gene

Start:
49,659,569 bp from pter
End:
49,665,875 bp from pter
Size:
6,307 bases
Orientation:
Minus strand

Genomic View for IRF3 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for IRF3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for IRF3 Gene

Proteins for IRF3 Gene

  • Protein details for IRF3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q14653-IRF3_HUMAN
    Recommended name:
    Interferon regulatory factor 3
    Protein Accession:
    Q14653
    Secondary Accessions:
    • A8K7L2
    • B2RAZ3
    • Q5FBY1
    • Q5FBY2
    • Q5FBY4
    • Q7Z5G6

    Protein attributes for IRF3 Gene

    Size:
    427 amino acids
    Molecular mass:
    47219 Da
    Quaternary structure:
    • Monomer. Homodimer; phosphorylation-induced. Heterodimer with IRF7. Interacts with CREBBP. May interact with MAVS. Interacts with IKBKE and TBK1. Interacts with TICAM1 and TICAM2. Interacts with RBCK1. Interacts with TRIM21. Interacts with HERC5. InteractS with DDX3X (phosphorylated at Ser-102); the interaction allows the phosphorylation and activation of IRF3 by IKBKE. Interacts with rotavirus A NSP1 (via C-terminus); this interaction leads to the proteasome-dependent degradation of IRF3. Interacts with herpes virus 8/HHV-8 protein vIRF-1.

    Three dimensional structures from OCA and Proteopedia for IRF3 Gene

    Alternative splice isoforms for IRF3 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for IRF3 Gene

Proteomics data for IRF3 Gene at MOPED

Post-translational modifications for IRF3 Gene

  • Constitutively phosphorylated on many Ser/Thr residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Phosphorylated at Ser-396 by IKBKE upon ssRNA viral infection. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. Phosphorylation at Ser-386 by TBK1 results in oligomerization. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection. Phosphorylation, and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3.
  • ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation.
  • Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation.
  • Ubiquitination at Lys70
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for IRF3 Gene

Domains for IRF3 Gene

Graphical View of Domain Structure for InterPro Entry

Q14653

UniProtKB/Swiss-Prot:

IRF3_HUMAN :
  • Q14653
Domain:
  • Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
Family:
  • Belongs to the IRF family.
genes like me logo Genes that share domains with IRF3: view

No data available for Gene Families for IRF3 Gene

Function for IRF3 Gene

Molecular function for IRF3 Gene

GENATLAS Biochemistry: interferon regulatory factor 3
UniProtKB/Swiss-Prot EnzymeRegulation: In the absence of viral infection, maintained as a monomer in an autoinhibited state and phosphorylation disrupts this autoinhibition leading to the liberation of the DNA-binding and dimerization activities and its nuclear localization where it can activate type I IFN and ISG genes
UniProtKB/Swiss-Prot Function: Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages

Gene Ontology (GO) - Molecular Function for IRF3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000975 regulatory region DNA binding --
GO:0003677 DNA binding NAS 12062447
GO:0003700 sequence-specific DNA binding transcription factor activity IEA --
GO:0003712 transcription cofactor activity TAS 8524823
GO:0005515 protein binding IPI 15841462
genes like me logo Genes that share ontologies with IRF3: view

Phenotypes for IRF3 Gene

genes like me logo Genes that share phenotypes with IRF3: view

Animal Models for IRF3 Gene

MGI Knock Outs for IRF3:

No data available for Enzyme Numbers (IUBMB) , miRNA , Transcription Factor Targeting and HOMER Transcription for IRF3 Gene

Localization for IRF3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for IRF3 Gene

Cytoplasm. Nucleus. Note=Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for IRF3 Gene COMPARTMENTS Subcellular localization image for IRF3 gene
Compartment Confidence
nucleus 5
cytosol 4
mitochondrion 4
endoplasmic reticulum 1
endosome 1
extracellular 1

Gene Ontology (GO) - Cellular Components for IRF3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA 17079289
GO:0005654 nucleoplasm TAS --
GO:0005737 cytoplasm IDA --
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with IRF3: view

Pathways for IRF3 Gene

genes like me logo Genes that share pathways with IRF3: view

Gene Ontology (GO) - Biological Process for IRF3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000122 negative regulation of transcription from RNA polymerase II promoter IEA --
GO:0002224 toll-like receptor signaling pathway TAS --
GO:0002756 MyD88-independent toll-like receptor signaling pathway TAS --
GO:0006355 regulation of transcription, DNA-templated --
GO:0006366 transcription from RNA polymerase II promoter TAS 8524823
genes like me logo Genes that share ontologies with IRF3: view

Compounds for IRF3 Gene

(17) Novoseek inferred chemical compound relationships for IRF3 Gene

Compound -log(P) Hits PubMed IDs
polyinosinic-polycytidylic acid 81.2 17
rantes 76.6 29
poly(i-c) 76.3 14
ifn-alpha 42.3 1
mdms 41.2 1
genes like me logo Genes that share compounds with IRF3: view

Transcripts for IRF3 Gene

Unigene Clusters for IRF3 Gene

Interferon regulatory factor 3:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for IRF3 Gene

ExUns: 1 ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6a · 6b · 6c ^ 7a · 7b ^ 8 ^ 9a · 9b ^ 10a · 10b · 10c
SP1: - - - -
SP2: - - - - - -
SP3: -
SP4:
SP5: - -
SP6: -
SP7:

Relevant External Links for IRF3 Gene

GeneLoc Exon Structure for
IRF3
ECgene alternative splicing isoforms for
IRF3

Expression for IRF3 Gene

mRNA expression in normal human tissues for IRF3 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for IRF3 Gene

SOURCE GeneReport for Unigene cluster for IRF3 Gene Hs.75254

mRNA Expression by UniProt/SwissProt for IRF3 Gene

Q14653-IRF3_HUMAN
Tissue specificity: Expressed constitutively in a variety of tissues
genes like me logo Genes that share expressions with IRF3: view

No data available for mRNA differential expression in normal tissues for IRF3 Gene

Orthologs for IRF3 Gene

This gene was present in the common ancestor of chordates.

Orthologs for IRF3 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia IRF3 36
  • 62 (a)
OneToOne
cow
(Bos Taurus)
Mammalia IRF3 35
  • 82.49 (n)
  • 76.74 (a)
IRF3 36
  • 63 (a)
OneToOne
dog
(Canis familiaris)
Mammalia IRF3 36
  • 58 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Irf3 35
  • 76.98 (n)
  • 72.42 (a)
Irf3 16
Irf3 36
  • 57 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia mdo-mir-7306 36
  • 39 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Irf3 35
  • 77.46 (n)
  • 75 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia LOC100493780 35
  • 48.46 (n)
  • 38.78 (a)
zebrafish
(Danio rerio)
Actinopterygii irf3 35
  • 44.89 (n)
  • 35.2 (a)
irf3 36
  • 22 (a)
OneToOne
Species with no ortholog for IRF3:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for IRF3 Gene

ENSEMBL:
Gene Tree for IRF3 (if available)
TreeFam:
Gene Tree for IRF3 (if available)

Paralogs for IRF3 Gene

Paralogs for IRF3 Gene

genes like me logo Genes that share paralogs with IRF3: view

Variants for IRF3 Gene

Sequence variations from dbSNP and Humsavar for IRF3 Gene

SNP ID Clin Chr 19 pos Sequence Context AA Info Type MAF
rs7251 - 49,659,652(-) GGAGA(C/G)CTGAG missense, reference, nc-transcript-variant
rs1049486 - 49,659,803(-) TGGTA(A/G)AAATG missense, reference, nc-transcript-variant
rs1130611 -- 49,660,731(-) ACCAA(A/G)AGGCT missense, reference, synonymous-codon, nc-transcript-variant
rs1140375 -- 49,661,737(-) TCGGG(C/T)GTGGT intron-variant, nc-transcript-variant
rs2230667 -- 49,662,445(-) GAAGC(A/G)GCTGT missense, reference, nc-transcript-variant

Structural Variations from Database of Genomic Variants (DGV) for IRF3 Gene

Variant ID Type Subtype PubMed ID
nsv9739 CNV Gain+Loss 18304495
nsv833864 CNV Gain 17160897
nsv828578 CNV Loss 20364138
nsv469715 CNV Complex 16826518
nsv833865 CNV Gain+Loss 17160897
dgv3972n71 CNV Loss 21882294
nsv912269 CNV Loss 21882294
nsv817854 CNV Gain 17921354
nsv470150 CNV Gain 18288195
dgv3973n71 CNV Loss 21882294

Relevant External Links for IRF3 Gene

HapMap Linkage Disequilibrium report
IRF3

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for IRF3 Gene

Disorders for IRF3 Gene

MalaCards: The human disease database

MalaCards: The human disease database. (2) Diseases for IRF3 Gene including...

Search for IRF3 Gene in MalaCards »

(1) University of Copenhagen DISEASES for IRF3 Gene

(21) Novoseek inferred disease relationships for IRF3 Gene

Disease -log(P) Hits PubMed IDs
virus infection 78.9 85
rna virus infections 63.8 3
vesicular stomatitis 61.6 4
necrosis 33.1 4
newcastle disease 31.2 1

Relevant External Links for IRF3

Genetic Association Database (GAD)
IRF3
Human Genome Epidemiology (HuGE) Navigator
IRF3
genes like me logo Genes that share disorders with IRF3: view

No data available for UniProtKB/Swiss-Prot for IRF3 Gene

Publications for IRF3 Gene

  1. Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes. (PMID: 8524823) Au W.W.-C. … Pitha P.M. (Proc. Natl. Acad. Sci. U.S.A. 1995) 2 3 4 23
  2. Regulated nuclear-cytoplasmic localization of interferon regulatory factor 3, a subunit of double-stranded RNA-activated factor 1. (PMID: 10805757) Kumar K.P. … Reich N.C. (Mol. Cell. Biol. 2000) 3 4 23
  3. Mapping of human interferon regulatory factor 3 (IRF3) to chromosome 19q13.3-13.4 by an intragenic polymorphic marker. (PMID: 9803267) Bellingham J. … Gregory-Evans C.Y. (Ann. Hum. Genet. 1998) 3 4 23
  4. Analyses of virus-induced homomeric and heteromeric protein associations between IRF-3 and coactivator CBP/p300. (PMID: 10920266) Suhara W. … Fujita T. (J. Biochem. 2000) 3 4 23
  5. HHV-8 encoded vIRF-1 represses the interferon antiviral response by blocking IRF-3 recruitment of the CBP/p300 coactivators. (PMID: 11314014) Lin R. … Hiscott J. (Oncogene 2001) 3 4 23

Products for IRF3 Gene

  • Addgene plasmids for IRF3

Sources for IRF3 Gene

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