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Aliases for IFI16 Gene

Aliases for IFI16 Gene

  • Interferon, Gamma-Inducible Protein 16 2 3
  • Interferon-Inducible Myeloid Differentiation Transcriptional Activator 3 4
  • IFNGIP1 3 4
  • Interferon-Gamma Induced Protein IFI 16 3
  • Gamma-Interferon-Inducible Protein 16 3
  • PYHIN2 3
  • Ifi-16 4

External Ids for IFI16 Gene

Summaries for IFI16 Gene

Entrez Gene Summary for IFI16 Gene

  • This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2011]

GeneCards Summary for IFI16 Gene

IFI16 (Interferon, Gamma-Inducible Protein 16) is a Protein Coding gene. Among its related pathways are Cytosolic sensors of pathogen-associated DNA and NF-kappaB Signaling. GO annotations related to this gene include transcription factor binding and double-stranded DNA binding. An important paralog of this gene is PYHIN1.

UniProtKB/Swiss-Prot for IFI16 Gene

  • Binds double-stranded DNA. Binds preferentially to supercoiled DNA and cruciform DNA structures. Seems to be involved in transcriptional regulation. May function as a transcriptional repressor. Could have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. May be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. Seems to be involved in energy-level-dependent activation of the ATM/ AMPK/TP53 pathway coupled to regulation of autophagy. May be involved in regulation of TP53-mediated cell death also involving BRCA1. May be involved in the senescence of prostate epithelial cells. Involved in innate immune response by recognizing viral dsDNA in the cytosol and probably in the nucleus. After binding to viral DNA in the cytoplasm recruits TMEM173/STING and mediates the induction of IFN-beta. Has anti-inflammatory activity and inhibits the activation of the AIM2 inflammasome, probably via association with AIM2. Proposed to bind viral DNA in the nucleus, such as of Kaposis sarcoma-associated herpesvirus, and to induce the formation of nuclear caspase-1-activating inflammasome formation via association with PYCARD. Inhibits replication of herpesviruses such as human cytomegalovirus (HCMV) probably by interfering with promoter recruitment of members of the Sp1 family of transcription factors. Necessary to activate the IRF3 signaling cascade during human herpes simplex virus 1 (HHV-1) infection and promotes the assembly of heterochromatin on herpesviral DNA and inhibition of viral immediate-early gene expression and replication. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.

Gene Wiki entry for IFI16 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for IFI16 Gene

Genomics for IFI16 Gene

Genomic Location for IFI16 Gene

Start:
158,999,968 bp from pter
End:
159,055,155 bp from pter
Size:
55,188 bases
Orientation:
Plus strand

Genomic View for IFI16 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for IFI16 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for IFI16 Gene

Regulatory Elements for IFI16 Gene

Proteins for IFI16 Gene

  • Protein details for IFI16 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q16666-IF16_HUMAN
    Recommended name:
    Gamma-interferon-inducible protein 16
    Protein Accession:
    Q16666
    Secondary Accessions:
    • B4DJT8
    • H3BLV7
    • Q59GX0
    • Q5T3W7
    • Q5T3W8
    • Q5T3X0
    • Q5T3X1
    • Q5T3X2
    • Q8N9E5
    • Q8NEQ7
    • Q96AJ5
    • Q9UH78

    Protein attributes for IFI16 Gene

    Size:
    785 amino acids
    Molecular mass:
    88256 Da
    Quaternary structure:
    • Forms homooligomers; isoform 2 can self-associate or associate with isoform 1 or isoform 3. Interacts with TMEM173, AIM2, PYCARD and CASP1. Interacts with BRCA1, TP53, E2F1, RB1 and SP1. Interacts with MTA1.
    SequenceCaution:
    • Sequence=AAF20997.1; Type=Frameshift; Positions=776; Evidence={ECO:0000305}; Sequence=AAF20997.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305}; Sequence=BAC04462.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact.; Evidence={ECO:0000305}; Sequence=BAD92226.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=BAG58950.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for IFI16 Gene

    Alternative splice isoforms for IFI16 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for IFI16 Gene

Proteomics data for IFI16 Gene at MOPED

Post-translational modifications for IFI16 Gene

  • Isoform 3 seems to show a minor degree of complex carbohydrate addition
  • Lysine acetylation in the multipartite nuclear localization signal (NLS) regulates the subcellular location. In vitro can be acetylated by p300/EP300 coupled to cytoplasmic localization.
  • Phosphorylated on Ser and Thr.
  • Ubiquitinated by human herpes simplex virus 1 (HHV-1) ICP0 protein; leading to degradation by the proteasome
  • Modification sites at PhosphoSitePlus
  • Ubiquitination at Lys128, Lys143, Lys190, Lys614, and Lys743

No data available for DME Specific Peptides for IFI16 Gene

Domains for IFI16 Gene

Protein Domains for IFI16 Gene

UniProtKB/Swiss-Prot:

IF16_HUMAN
Domain:
  • The HIN-20 domains mediates dsDNA binding via electrostatic interactions.:
    • Q16666
  • Contains 1 DAPIN domain.:
    • Q16666
  • Contains 2 HIN-200 domains.:
    • Q16666
Family:
  • Belongs to the HIN-200 family.:
    • Q16666
genes like me logo Genes that share domains with IFI16: view

No data available for Gene Families for IFI16 Gene

Function for IFI16 Gene

Molecular function for IFI16 Gene

GENATLAS Biochemistry: nuclear protein,constitutively expressed in lymphocytes,interferon alpha or gamma inducible in myeloid cells (clone IFI-6-16)
UniProtKB/Swiss-Prot Function: Binds double-stranded DNA. Binds preferentially to supercoiled DNA and cruciform DNA structures. Seems to be involved in transcriptional regulation. May function as a transcriptional repressor. Could have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. May be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. Seems to be involved in energy-level-dependent activation of the ATM/ AMPK/TP53 pathway coupled to regulation of autophagy. May be involved in regulation of TP53-mediated cell death also involving BRCA1. May be involved in the senescence of prostate epithelial cells. Involved in innate immune response by recognizing viral dsDNA in the cytosol and probably in the nucleus. After binding to viral DNA in the cytoplasm recruits TMEM173/STING and mediates the induction of IFN-beta. Has anti-inflammatory activity and inhibits the activation of the AIM2 inflammasome, probably via association with AIM2. Proposed to bind viral DNA in the nucleus, such as of Kaposis sarcoma-associated herpesvirus, and to induce the formation of nuclear caspase-1-activating inflammasome formation via association with PYCARD. Inhibits replication of herpesviruses such as human cytomegalovirus (HCMV) probably by interfering with promoter recruitment of members of the Sp1 family of transcription factors. Necessary to activate the IRF3 signaling cascade during human herpes simplex virus 1 (HHV-1) infection and promotes the assembly of heterochromatin on herpesviral DNA and inhibition of viral immediate-early gene expression and replication. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.
UniProtKB/Swiss-Prot Induction: Strongly induced by IFNG/IFN-gamma and, to a lesser extent, by alpha interferon. In HL-60 cells, maximum induction by IFNG/IFN-gamma occurs within 12 hours whereas, for IFN-alpha, only 10-fold induction was observed after 36 hours. Induced in vitro by dimethylsulfoxide, retinoic acid and 1,25 dihydroxyvitamin D3. Induced in monocytes by IFN-alpha and viral dsDNA. Induced by glucose restriction.

Gene Ontology (GO) - Molecular Function for IFI16 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000978 RNA polymerase II core promoter proximal region sequence-specific DNA binding IDA 24413532
GO:0001047 core promoter binding IDA 24413532
GO:0001078 RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription IDA 24413532
GO:0003690 double-stranded DNA binding IDA 7536752
GO:0005515 protein binding IPI 11146555
genes like me logo Genes that share ontologies with IFI16: view

Phenotypes for IFI16 Gene

GenomeRNAi human phenotypes for IFI16:
genes like me logo Genes that share phenotypes with IFI16: view

miRNA for IFI16 Gene

No data available for Enzyme Numbers (IUBMB) , Animal Models , Transcription Factor Targeting and HOMER Transcription for IFI16 Gene

Localization for IFI16 Gene

Subcellular locations from UniProtKB/Swiss-Prot for IFI16 Gene

Nucleus. Cytoplasm. Note=Cellular distribution is dependent on the acetylation status of the multipartite nuclear localization signal (NLS); NLS acetylation promotes cytoplasmic localization. Localizes in the nucleus during human herpes simplex virus 1 (HHV-1) infection.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for IFI16 Gene COMPARTMENTS Subcellular localization image for IFI16 gene
Compartment Confidence
nucleus 5
cytosol 4

Gene Ontology (GO) - Cellular Components for IFI16 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA --
GO:0005654 nucleoplasm IDA 14654789
GO:0005730 nucleolus IDA --
GO:0005737 cytoplasm IDA --
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with IFI16: view

Pathways for IFI16 Gene

genes like me logo Genes that share pathways with IFI16: view

Pathways by source for IFI16 Gene

1 Cell Signaling Technology pathway for IFI16 Gene
2 BioSystems pathways for IFI16 Gene

Gene Ontology (GO) - Biological Process for IFI16 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000122 negative regulation of transcription from RNA polymerase II promoter IMP 22046441
GO:0001819 positive regulation of cytokine production TAS 22046441
GO:0002218 activation of innate immune response IDA 21575908
GO:0006351 transcription, DNA-templated IEA --
GO:0006914 autophagy IEA --
genes like me logo Genes that share ontologies with IFI16: view

Compounds for IFI16 Gene

(3) Novoseek inferred chemical compound relationships for IFI16 Gene

Compound -log(P) Hits PubMed IDs
mnda 97.8 17
p202 94.3 2
physostigmine 67.6 2
genes like me logo Genes that share compounds with IFI16: view

Transcripts for IFI16 Gene

Unigene Clusters for IFI16 Gene

Interferon, gamma-inducible protein 16:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for IFI16 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b · 6c ^ 7a · 7b ^ 8a · 8b · 8c ^ 9a · 9b ^ 10 ^ 11a · 11b · 11c · 11d ^ 12 ^ 13 ^ 14a · 14b · 14c ^
SP1:
SP2: -
SP3: - -
SP4:
SP5: - -
SP6: -
SP7: -
SP8: - - - -
SP9: -
SP10:
SP11: - - -
SP12:

ExUns: 15a · 15b · 15c ^ 16a · 16b
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10: -
SP11:
SP12:

Relevant External Links for IFI16 Gene

GeneLoc Exon Structure for
IFI16
ECgene alternative splicing isoforms for
IFI16

Expression for IFI16 Gene

mRNA expression in normal human tissues for IFI16 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for IFI16 Gene

This gene is overexpressed in Whole Blood (5.9).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for IFI16 Gene

SOURCE GeneReport for Unigene cluster for IFI16 Gene Hs.380250

mRNA Expression by UniProt/SwissProt for IFI16 Gene

Q16666-IF16_HUMAN
Tissue specificity: Expressed in peripheral blood leukocytes, fibroblasts and lymphoid cells. Present in myeloid precursors (CD34+) and throughout monocyte development, but its expression is down-regulated in erythroid and polymorphonuclear precursor cells. Present in prostate, ovary and breast (at protein level).
genes like me logo Genes that share expressions with IFI16: view

Orthologs for IFI16 Gene

This gene was present in the common ancestor of mammals.

Orthologs for IFI16 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia IFI16 36
  • 99.27 (n)
  • 98.35 (a)
IFI16 37
  • 98 (a)
OneToOne
cow
(Bos Taurus)
Mammalia -- 37
  • 31 (a)
OneToMany
dog
(Canis familiaris)
Mammalia -- 37
  • 21 (a)
ManyToMany
mouse
(Mus musculus)
Mammalia BC094916 37
  • 37 (a)
ManyToMany
Ifi204 16
Pydc4 36
  • 65.77 (n)
  • 57.14 (a)
oppossum
(Monodelphis domestica)
Mammalia -- 37
  • 30 (a)
OneToMany
rat
(Rattus norvegicus)
Mammalia Mnda 36
  • 65.29 (n)
  • 47.55 (a)
Species with no ortholog for IFI16:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)
  • zebrafish (Danio rerio)

Evolution for IFI16 Gene

ENSEMBL:
Gene Tree for IFI16 (if available)
TreeFam:
Gene Tree for IFI16 (if available)

Paralogs for IFI16 Gene

Paralogs for IFI16 Gene

Selected SIMAP similar genes for IFI16 Gene using alignment to 7 proteins:

genes like me logo Genes that share paralogs with IFI16: view

Variants for IFI16 Gene

Sequence variations from dbSNP and Humsavar for IFI16 Gene

SNP ID Clin Chr 01 pos Sequence Context AA Info Type MAF
rs1956 -- 159,019,137(-) TCCTA(C/T)CTACT intron-variant
rs6940 -, - 159,054,878(+) TGGAA(A/T)CTTCA reference, missense
rs855864 -- 159,055,617(+) ATATT(C/T)CCTGT downstream-variant-500B
rs856042 -- 159,016,435(+) AAACA(C/T)CTTCT intron-variant
rs856043 -- 159,017,451(-) AAAAA(C/T)GGTGC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for IFI16 Gene

Variant ID Type Subtype PubMed ID
nsv3176 CNV Loss 18451855
nsv872486 CNV Loss 21882294
esv32912 CNV Loss 17666407
nsv506961 CNV Insertion 20534489
esv33390 CNV Gain 17666407
esv33763 CNV Loss 17666407
esv2718684 CNV Deletion 23290073
esv6708 CNV Loss 19470904
esv21466 CNV Gain+Loss 19812545
nsv825019 CNV Loss 20364138
nsv825032 CNV Loss 20364138
esv2718696 CNV Deletion 23290073

Relevant External Links for IFI16 Gene

HapMap Linkage Disequilibrium report
IFI16

Disorders for IFI16 Gene

(4) Novoseek inferred disease relationships for IFI16 Gene

Disease -log(P) Hits PubMed IDs
autoimmune diseases 38.7 1
melanoma 28.1 3
cancer 2.15 1
tumors 0 10

Relevant External Links for IFI16

Genetic Association Database (GAD)
IFI16
Human Genome Epidemiology (HuGE) Navigator
IFI16
genes like me logo Genes that share disorders with IFI16: view

No data available for UniProtKB/Swiss-Prot for IFI16 Gene

Publications for IFI16 Gene

  1. A novel gene constitutively expressed in human lymphoid cells is inducible with interferon-gamma in myeloid cells. (PMID: 1526658) Trapani J.A. … Dupont B. (Immunogenetics 1992) 2 3 4 23
  2. Genomic organization of IFI16, an interferon-inducible gene whose expression is associated with human myeloid cell differentiation: correlation of predicted protein domains with exon organization. (PMID: 7959953) Trapani J.A. … Browne K.A. (Immunogenetics 1994) 2 3 4 23
  3. IFI 16 gene encodes a nuclear protein whose expression is induced by interferons in human myeloid leukaemia cell lines. (PMID: 7536752) Dawson M.J. … Trapani J.A. (J. Cell. Biochem. 1995) 3 4 23
  4. The human interferon-inducible protein, IFI 16, is a repressor of transcription. (PMID: 9642285) Johnstone R.W. … Trapani J.A. (J. Biol. Chem. 1998) 3 4 23
  5. Isotypic variants of the interferon-inducible transcriptional repressor IFI 16 arise through differential mRNA splicing. (PMID: 9718316) Johnstone R.W. … Trapani J.A. (Biochemistry 1998) 3 4 23

Products for IFI16 Gene

  • Pathway & Disease-focused RT² Profiler PCR Arrays
    • Apoptosis 384HT in human,mouse,rat
    • Interferons & Receptors in human,mouse,rat
    • Type I Interferon Response in human,mouse,rat
  • QIAGEN qRT-PCR Assays for microRNAs that regulate IFI16
  • QuantiTect SYBR Green Assays in human,mouse,rat
  • Pre-validated RT² qPCR Primer Assay in human,mouse,rat
  • QuantiFast Probe-based Assays in human,mouse,rat
  • Predesigned siRNA for gene silencing in human,mouse,rat for IFI16
  • Block miRNA regulation of IFI16 using miScript Target Protectors

Sources for IFI16 Gene

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