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Aliases for TSSK2 Gene

Aliases for TSSK2 Gene

  • Testis-Specific Serine Kinase 2 2 3
  • STK22B 3 4 6
  • Serine/Threonine Kinase 22B (Spermiogenesis Associated) 2 3
  • Serine/Threonine-Protein Kinase 22B 3 4
  • DiGeorge Syndrome Protein G 3 4
  • Testis-Specific Kinase 2 3 4
  • EC 4 64
  • SPOGA2 3 4
  • TSSK-2 3 4
  • DGS-G 3 4
  • TSK-2 3 4
  • TSK2 3 4
  • Testis-Specific Serine/Threonine-Protein Kinase 2 3
  • Testis Specific Serine Threonine Kinase 2 3
  • Spermiogenesis Associated 2 3
  • EC 2.7.11 64
  • DGSG 4

External Ids for TSSK2 Gene

Previous Symbols for TSSK2 Gene

  • STK22B

Summaries for TSSK2 Gene

Entrez Gene Summary for TSSK2 Gene

  • TSSK2 belongs to a family of serine/threonine kinases highly expressed in testis (Hao et al., 2004 [PubMed 15044604]).[supplied by OMIM, Mar 2008]

GeneCards Summary for TSSK2 Gene

TSSK2 (Testis-Specific Serine Kinase 2) is a Protein Coding gene. Diseases associated with TSSK2 include digeorge syndrome. Among its related pathways are Sweet Taste Signaling and Transcription_CREM signaling in testis. GO annotations related to this gene include identical protein binding and magnesium ion binding. An important paralog of this gene is TSSK4.

UniProtKB/Swiss-Prot for TSSK2 Gene

  • Testis-specific serine/threonine-protein kinase required during spermatid development. Phosphorylates TSKS at Ser-288 and SPAG16. Involved in the late stages of spermatogenesis, during the reconstruction of the cytoplasm. During spermatogenesis, required for the transformation of a ring-shaped structure around the base of the flagellum originating from the chromatoid body.

Gene Wiki entry for TSSK2 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for TSSK2 Gene

Genomics for TSSK2 Gene

Genomic Location for TSSK2 Gene

19,130,808 bp from pter
19,132,623 bp from pter
1,816 bases
Plus strand

Genomic View for TSSK2 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for TSSK2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for TSSK2 Gene

Regulatory Elements for TSSK2 Gene

Proteins for TSSK2 Gene

  • Protein details for TSSK2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Testis-specific serine/threonine-protein kinase 2
    Protein Accession:
    Secondary Accessions:
    • Q8IY55

    Protein attributes for TSSK2 Gene

    358 amino acids
    Molecular mass:
    40939 Da
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Interacts with TSSK1B.
    • TSSK1B might be used as a target for male contraception or and intra-vaginal spermicides.

neXtProt entry for TSSK2 Gene

Proteomics data for TSSK2 Gene at MOPED

Post-translational modifications for TSSK2 Gene

Other Protein References for TSSK2 Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains for TSSK2 Gene


  • Contains 1 protein kinase domain.:
    • Q96PF2
  • Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.:
    • Q96PF2
genes like me logo Genes that share domains with TSSK2: view

Function for TSSK2 Gene

Molecular function for TSSK2 Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties: Kinetic parameters: KM=10 uM for ATP {ECO:0000269 PubMed:20729278};
UniProtKB/Swiss-Prot CatalyticActivity: ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation: Activated by phosphorylation on Thr-174, potentially by autophosphorylation.
UniProtKB/Swiss-Prot Function: Testis-specific serine/threonine-protein kinase required during spermatid development. Phosphorylates TSKS at Ser-288 and SPAG16. Involved in the late stages of spermatogenesis, during the reconstruction of the cytoplasm. During spermatogenesis, required for the transformation of a ring-shaped structure around the base of the flagellum originating from the chromatoid body.

Enzyme Numbers (IUBMB) for TSSK2 Gene

Gene Ontology (GO) - Molecular Function for TSSK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000287 magnesium ion binding ISS --
GO:0004672 protein kinase activity --
GO:0004674 protein serine/threonine kinase activity ISS --
GO:0004713 protein tyrosine kinase activity --
GO:0005515 protein binding IPI 15044604
genes like me logo Genes that share ontologies with TSSK2: view
genes like me logo Genes that share phenotypes with TSSK2: view

Animal Models for TSSK2 Gene

MGI Knock Outs for TSSK2:

No data available for miRNA , Transcription Factor Targeting and HOMER Transcription for TSSK2 Gene

Localization for TSSK2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for TSSK2 Gene

Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Note=Present in the cytoplasm of elongating spermatids. In spermatozoa, localizes in the equatorial segment, neck, the midpiece and in a specific sperm head compartment (By similarity). In spermatids, concentrates in centrioles during flagellogenesis. {ECO:0000250}.

Subcellular locations from

Jensen Localization Image for TSSK2 Gene COMPARTMENTS Subcellular localization image for TSSK2 gene
Compartment Confidence
cytoskeleton 5
cytosol 3
nucleus 2
extracellular 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for TSSK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001669 acrosomal vesicle IEA --
GO:0005634 nucleus IDA 21630459
GO:0005737 cytoplasm ISS --
GO:0005814 centriole IEA --
genes like me logo Genes that share ontologies with TSSK2: view

Pathways for TSSK2 Gene

genes like me logo Genes that share pathways with TSSK2: view

Pathways by source for TSSK2 Gene

1 GeneGo (Thomson Reuters Life Sciences Research) pathway for TSSK2 Gene
1 Qiagen pathway for TSSK2 Gene

Gene Ontology (GO) - Biological Process for TSSK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006468 protein phosphorylation ISS --
GO:0007275 multicellular organismal development IEA --
GO:0007286 spermatid development ISS --
GO:0035556 intracellular signal transduction IBA --
GO:0046777 protein autophosphorylation IDA 20729278
genes like me logo Genes that share ontologies with TSSK2: view

Compounds for TSSK2 Gene

(3) HMDB Compounds for TSSK2 Gene

Compound Synonyms Cas Number PubMed IDs
Adenosine triphosphate
  • 5'-(tetrahydrogen triphosphate) Adenosine
  • adenosindiphosphorsaeure
  • Magnesium
genes like me logo Genes that share compounds with TSSK2: view

Transcripts for TSSK2 Gene

mRNA/cDNA for TSSK2 Gene

(1) REFSEQ mRNAs :
(4) Additional mRNA sequences :
(1) Selected AceView cDNA sequences:
(1) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for TSSK2 Gene

Testis-specific serine kinase 2:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for TSSK2 Gene

No ASD Table

Relevant External Links for TSSK2 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for TSSK2 Gene

mRNA expression in normal human tissues for TSSK2 Gene

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, and MOPED for TSSK2 Gene

SOURCE GeneReport for Unigene cluster for TSSK2 Gene Hs.694070

mRNA Expression by UniProt/SwissProt for TSSK2 Gene

Tissue specificity: Testis-specific. Present in mature spermatozoa (at protein level).
genes like me logo Genes that share expressions with TSSK2: view

Orthologs for TSSK2 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for TSSK2 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia TSSK2 36
  • 99.53 (n)
  • 98.6 (a)
TSSK2 37
  • 99 (a)
(Bos Taurus)
Mammalia TSSK2 36
  • 89.2 (n)
  • 91.34 (a)
TSSK2 37
  • 91 (a)
(Canis familiaris)
Mammalia TSSK2 36
  • 86.69 (n)
  • 87.43 (a)
TSSK2 37
  • 94 (a)
(Mus musculus)
Mammalia Tssk2 36
  • 89.48 (n)
  • 92.18 (a)
Tssk2 16
Tssk2 37
  • 92 (a)
(Monodelphis domestica)
Mammalia TSSK2 37
  • 89 (a)
(Ornithorhynchus anatinus)
Mammalia TSSK2 37
  • 80 (a)
(Rattus norvegicus)
Mammalia Tssk2 36
  • 89.48 (n)
  • 93.02 (a)
(Anolis carolinensis)
Reptilia -- 37
  • 72 (a)
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.4797 36
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP008765 36
  • 53.21 (n)
  • 41.85 (a)
(Caenorhabditis elegans)
Secernentea tag-344 37
  • 30 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes -- 37
  • 11 (a)
FRK1 37
  • 13 (a)
KIN4 37
  • 14 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.4544 37
  • 50 (a)
Species with no ortholog for TSSK2:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • zebrafish (Danio rerio)

Evolution for TSSK2 Gene

Gene Tree for TSSK2 (if available)
Gene Tree for TSSK2 (if available)

Paralogs for TSSK2 Gene

Paralogs for TSSK2 Gene

Selected SIMAP similar genes for TSSK2 Gene using alignment to 2 proteins:

genes like me logo Genes that share paralogs with TSSK2: view

Variants for TSSK2 Gene

Sequence variations from dbSNP and Humsavar for TSSK2 Gene

SNP ID Clin Chr 22 pos Sequence Context AA Info Type MAF
rs5747995 -- 19,129,441(+) AGGCA(G/T)CCCGA upstream-variant-2KB
rs8139221 -- 19,129,754(+) ACTCT(C/T)TCTGT upstream-variant-2KB
rs17811040 -- 19,128,838(+) CAGCC(A/G)TAGAG upstream-variant-2KB
rs34517131 -- 19,129,609(+) CCAAG(-/G)CCCAT upstream-variant-2KB
rs41277599 -- 19,129,311(+) ATGCC(C/T)GTGAT upstream-variant-2KB

Structural Variations from Database of Genomic Variants (DGV) for TSSK2 Gene

Variant ID Type Subtype PubMed ID
nsv435744 CNV Deletion 17901297
nsv914263 CNV Gain 21882294
nsv914268 CNV Gain 21882294
nsv834122 CNV Loss 17160897

Relevant External Links for TSSK2 Gene

HapMap Linkage Disequilibrium report
Human Gene Mutation Database (HGMD)

Disorders for TSSK2 Gene

(1) MalaCards Diseases for TSSK2 Gene

Search for TSSK2 Gene in MalaCards »

(1) University of Copenhagen DISEASES for TSSK2 Gene

Relevant External Links for TSSK2

Genetic Association Database (GAD)
Human Genome Epidemiology (HuGE) Navigator
genes like me logo Genes that share disorders with TSSK2: view

No data available for UniProtKB/Swiss-Prot for TSSK2 Gene

Publications for TSSK2 Gene

  1. The DNA sequence of human chromosome 22. (PMID: 10591208) Dunham I. … Wright H. (Nature 1999) 2 3 4
  2. Some single-nucleotide polymorphisms of the TSSK2 gene may be associated with human spermatogenesis impairment. (PMID: 19926886) Zhang H. … Zhang S. (J. Androl. 2010) 3 4 49
  3. Expression and localization of five members of the testis-specific serine kinase (Tssk) family in mouse and human sperm and testis. (PMID: 20729278) Li Y. … Salicioni A.M. (Mol. Hum. Reprod. 2011) 3 4
  4. A novel family of serine/threonine kinases participating in spermiogenesis. (PMID: 9412477) Kueng P. … Ziemiecki A. (J. Cell Biol. 1997) 3 23
  5. Validation of a testis specific serine/threonine kinase [TSSK] family and the substrate of TSSK1 & 2, TSKS, as contraceptive targets. (PMID: 17566264) Xu B. … Herr J.C. (Soc. Reprod. Fertil. Suppl. 2007) 4 23

Products for TSSK2 Gene

  • Addgene plasmids for TSSK2

Sources for TSSK2 Gene

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