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Aliases for ISCU Gene

Aliases for ISCU Gene

  • Iron-Sulfur Cluster Assembly Enzyme 2 3
  • NifU-Like N-Terminal Domain-Containing Protein 3 4
  • NIFUN 3 4
  • IscU Iron-Sulfur Cluster Scaffold Homolog (E. Coli) 2
  • Iron-Sulfur Cluster Scaffold Homolog (E. Coli) 2
  • IscU Iron-Sulfur Cluster Scaffold Homolog 3
  • NifU-Like N-Terminal Domain Containing 2
  • NifU-Like Protein 4
  • 2310020H20Rik 3
  • HnifU 3
  • NifU 3
  • ISU2 3
  • HML 3

External Ids for ISCU Gene

Previous HGNC Symbols for ISCU Gene


Previous GeneCards Identifiers for ISCU Gene

  • GC12P107458
  • GC12P107459
  • GC12P108956
  • GC12P106020

Summaries for ISCU Gene

Entrez Gene Summary for ISCU Gene

  • Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. They contain sulfur and iron, and are created via several steps that include cysteine desulfurases, iron donors, chaperones, and scaffold proteins. This gene encodes the two isomeric forms, ISCU1 and ISCU2, of the Fe-S cluster scaffold protein. Mutations in this gene have been found in patients with myopathy with severe exercise intolerance and myoglobinuria. A pseudogene of this gene is present on chromosome 1. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]

GeneCards Summary for ISCU Gene

ISCU (Iron-Sulfur Cluster Assembly Enzyme) is a Protein Coding gene. Diseases associated with ISCU include myopathy with deficiency of iron-sulfur cluster assembly enzyme and hereditary myopathy with lactic acidosis due to iscu deficiency. Among its related pathways are Metabolism and Mitochondrial iron-sulfur cluster biogenesis. GO annotations related to this gene include iron ion binding and iron-sulfur cluster binding.

UniProtKB/Swiss-Prot for ISCU Gene

  • Involved in the assembly or repair of the [Fe-S] clusters present in iron-sulfur proteins. Binds iron.

Gene Wiki entry for ISCU Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for ISCU Gene

Genomics for ISCU Gene

Regulatory Elements for ISCU Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for ISCU Gene

108,561,463 bp from pter
108,569,384 bp from pter
7,922 bases
Plus strand

Genomic View for ISCU Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
GeneLoc Logo Gene Density

RefSeq DNA sequence for ISCU Gene

Proteins for ISCU Gene

  • Protein details for ISCU Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Iron-sulfur cluster assembly enzyme ISCU, mitochondrial
    Protein Accession:
    Secondary Accessions:
    • Q6P713
    • Q99617
    • Q9H1K2

    Protein attributes for ISCU Gene

    167 amino acids
    Molecular mass:
    17999 Da
    Quaternary structure:
    • Binds NFS1. Interacts with HSCB.

    Alternative splice isoforms for ISCU Gene


neXtProt entry for ISCU Gene

Proteomics data for ISCU Gene at MOPED

Post-translational modifications for ISCU Gene

  • Ubiquitination at Lys 54, Lys 57, Lys 82, Lys 91, Lys 112, Lys 121, and Lys 147
  • Modification sites at PhosphoSitePlus

Antibody Products

No data available for DME Specific Peptides for ISCU Gene

Domains & Families for ISCU Gene

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the NifU family.
  • Belongs to the NifU family.
genes like me logo Genes that share domains with ISCU: view

No data available for Gene Families for ISCU Gene

Function for ISCU Gene

Molecular function for ISCU Gene

UniProtKB/Swiss-Prot Function:
Involved in the assembly or repair of the [Fe-S] clusters present in iron-sulfur proteins. Binds iron.

Gene Ontology (GO) - Molecular Function for ISCU Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005506 iron ion binding TAS 11060020
GO:0005515 protein binding IPI 16527810
GO:0008198 ferrous iron binding IBA --
GO:0032947 protein complex scaffold IDA 16527810
GO:0036455 iron-sulfur transferase activity IBA --
genes like me logo Genes that share ontologies with ISCU: view
genes like me logo Genes that share phenotypes with ISCU: view

miRNA for ISCU Gene

miRTarBase miRNAs that target ISCU

No data available for Enzyme Numbers (IUBMB) , Animal Models , Transcription Factor Targets and HOMER Transcription for ISCU Gene

Localization for ISCU Gene

Subcellular locations from UniProtKB/Swiss-Prot for ISCU Gene

Isoform 1: Mitochondrion.
Isoform 2: Cytoplasm. Nucleus.

Subcellular locations from

Jensen Localization Image for ISCU Gene COMPARTMENTS Subcellular localization image for ISCU gene
Compartment Confidence
mitochondrion 5
nucleus 5
cytosol 4

Gene Ontology (GO) - Cellular Components for ISCU Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus TAS 16527810
GO:0005737 cytoplasm IDA 11060020
GO:0005739 mitochondrion IDA 11060020
GO:0005759 mitochondrial matrix TAS --
GO:0005829 cytosol IDA 16527810
genes like me logo Genes that share ontologies with ISCU: view

Pathways & Interactions for ISCU Gene

genes like me logo Genes that share pathways with ISCU: view

Pathways by source for ISCU Gene

2 Reactome pathways for ISCU Gene

SIGNOR curated interactions for ISCU Gene

Is activated by:

Gene Ontology (GO) - Biological Process for ISCU Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006879 cellular iron ion homeostasis IBA --
GO:0009399 nitrogen fixation TAS 8875867
GO:0016226 iron-sulfur cluster assembly TAS 11060020
GO:0044281 small molecule metabolic process TAS --
GO:0044571 [2Fe-2S] cluster assembly IBA --
genes like me logo Genes that share ontologies with ISCU: view

Drugs & Compounds for ISCU Gene

(2) Drugs for ISCU Gene - From: NovoSeek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Iron Approved Pharma 957
cysteine Nutra 143

(3) Additional Compounds for ISCU Gene - From: HMDB and NovoSeek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
  • Armco iron
  • Carbonyl iron
  • FE
  • Ferrovac e
  • Hematite
genes like me logo Genes that share compounds with ISCU: view

Transcripts for ISCU Gene

Unigene Clusters for ISCU Gene

Iron-sulfur cluster scaffold homolog (E. coli):
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for ISCU Gene

ExUns: 1a · 1b · 1c · 1d · 1e ^ 2a · 2b ^ 3 ^ 4a · 4b ^ 5a · 5b · 5c ^ 6 ^ 7 ^ 8a · 8b · 8c · 8d
SP1: - - - - - -
SP2: - - - - - -
SP3: - - - - - -
SP4: - - - - - - -
SP5: - - -
SP6: - -
SP7: - - -

Relevant External Links for ISCU Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for ISCU Gene

mRNA expression in normal human tissues for ISCU Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for ISCU Gene

This gene is overexpressed in Peripheral blood mononuclear cells (11.1) and Heart (7.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for ISCU Gene

SOURCE GeneReport for Unigene cluster for ISCU Gene Hs.615131

mRNA Expression by UniProt/SwissProt for ISCU Gene

Tissue specificity: Detected in heart, liver, skeletal muscle, brain, pancreas, kidney, lung and placenta.
genes like me logo Genes that share expression patterns with ISCU: view

Protein tissue co-expression partners for ISCU Gene

- Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA differential expression in normal tissues for ISCU Gene

Orthologs for ISCU Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for ISCU Gene

Organism Taxonomy Gene Similarity Type Details
(Bos Taurus)
Mammalia ISCU 35
  • 90.02 (n)
  • 97.6 (a)
  • 98 (a)
(Canis familiaris)
Mammalia LOC477527 35
  • 93.41 (n)
  • 98.8 (a)
  • 99 (a)
(Mus musculus)
Mammalia Iscu 35
  • 87.55 (n)
  • 96.39 (a)
Iscu 16
Iscu 36
  • 95 (a)
(Pan troglodytes)
Mammalia ISCU 35
  • 99.6 (n)
  • 99.4 (a)
-- 36
  • 99 (a)
  • 99 (a)
(Rattus norvegicus)
Mammalia Iscu 35
  • 88.22 (n)
  • 97.01 (a)
(Monodelphis domestica)
Mammalia ISCU 36
  • 83 (a)
(Ornithorhynchus anatinus)
Mammalia ISCU 36
  • 56 (a)
(Gallus gallus)
Aves ISCU 35
  • 75 (n)
  • 85.53 (a)
  • 78 (a)
(Anolis carolinensis)
Reptilia ISCU 36
  • 81 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia iscu 35
  • 78.45 (n)
  • 84.96 (a)
African clawed frog
(Xenopus laevis)
Amphibia nifu-pending-prov 35
(Danio rerio)
Actinopterygii iscub 35
  • 73.43 (n)
  • 86.47 (a)
wufj08f02 35
ISCU (2 of 2) 36
  • 77 (a)
iscub 36
  • 71 (a)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP005813 35
  • 69.08 (n)
  • 80.43 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG9836 35
  • 68.28 (n)
  • 79.31 (a)
CG9836 36
  • 75 (a)
(Caenorhabditis elegans)
Secernentea Y45F10D.4 35
  • 63.7 (n)
  • 71.23 (a)
Y45F10D.4 36
  • 68 (a)
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_ACR112C 35
  • 60.69 (n)
  • 66.21 (a)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0D07161g 35
  • 64.49 (n)
  • 71.74 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes ISU2 35
  • 65.9 (n)
  • 72.31 (a)
ISU1 36
  • 60 (a)
ISU2 36
  • 62 (a)
ISU2 38
thale cress
(Arabidopsis thaliana)
eudicotyledons ISU3 35
  • 65.15 (n)
  • 74.24 (a)
Alicante grape
(Vitis vinifera)
eudicotyledons Vvi.6801 35
(Oryza sativa)
Liliopsida Os01g0662600 35
  • 65.91 (n)
  • 77.27 (a)
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.4350 35
bread mold
(Neurospora crassa)
Ascomycetes NCU05778 35
  • 59.33 (n)
  • 68 (a)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes isu1 35
  • 62.93 (n)
  • 71.2 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 70 (a)
Species with no ortholog for ISCU:
  • Actinobacteria (Mycobacterium tuberculosis)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for ISCU Gene

Gene Tree for ISCU (if available)
Gene Tree for ISCU (if available)

Paralogs for ISCU Gene Pseudogenes for ISCU Gene

genes like me logo Genes that share paralogs with ISCU: view

No data available for Paralogs for ISCU Gene

Variants for ISCU Gene

Sequence variations from dbSNP and Humsavar for ISCU Gene

SNP ID Clin Chr 12 pos Sequence Context AA Info Type MAF
rs10187 -- 108,569,028(+) AGATA(C/T)GCACA utr-variant-3-prime, downstream-variant-500B
rs876161 -- 108,567,452(+) AGAGA(A/G)TCATG intron-variant
rs880844 -- 108,568,592(-) TAGTT(C/T)CTTAG intron-variant, nc-transcript-variant
rs888546 -- 108,567,517(+) TAAAC(C/T)TCACA intron-variant
rs888547 -- 108,567,578(+) GAAGC(C/T)AAGGC intron-variant

Variation tolerance for ISCU Gene

Residual Variation Intolerance Score: 45.81% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.30; 25.83% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for ISCU Gene

HapMap Linkage Disequilibrium report
Human Gene Mutation Database (HGMD)

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Structural Variations from Database of Genomic Variants (DGV) for ISCU Gene

Disorders for ISCU Gene

MalaCards: The human disease database

(7) MalaCards diseases for ISCU Gene - From: OMIM, ClinVar, GeneTests, Orphanet, DISEASES, and GeneCards

Disorder Aliases PubMed IDs
myopathy with deficiency of iron-sulfur cluster assembly enzyme
  • myopathy with deficiency of iscu
hereditary myopathy with lactic acidosis due to iscu deficiency
  • iron-sulfur cluster deficiency myopathy
myopathy with lactic acidosis, hereditary
  • myopathy with exercise intolerance, swedish type
myopathy with deficiency of iscu
  • iron-sulfur cluster deficiency myopathy
  • pulmonary siderosis
- elite association
Search ISCU in MalaCards View complete list of genes associated with diseases


  • Myopathy with exercise intolerance Swedish type (MEIS) [MIM:255125]: Autosomal recessive metabolic disease characterized by lifelong severe exercise intolerance, in which minor exertion causes fatigue of active muscles, shortness of breath, and cardiac palpitations in association with lactic acidosis. The biochemical phenotype is characterized by a deficiency in mitochondrial iron-sulfur proteins and impaired muscle oxidative metabolism. {ECO:0000269 PubMed:18304497}. Note=The disease is caused by mutations affecting the gene represented in this entry.

Relevant External Links for ISCU

Genetic Association Database (GAD)
Human Genome Epidemiology (HuGE) Navigator
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
genes like me logo Genes that share disorders with ISCU: view

No data available for Genatlas for ISCU Gene

Publications for ISCU Gene

  1. Distinct iron-sulfur cluster assembly complexes exist in the cytosol and mitochondria of human cells. (PMID: 11060020) Tong W.-H. … Rouault T. (EMBO J. 2000) 2 23 67
  2. A modular domain of NifU, a nitrogen fixation cluster protein, is highly conserved in evolution. (PMID: 8875867) Hwang D.M. … Liew C.-C. (J. Mol. Evol. 1996) 2 23 67
  3. Iron-binding activity of human iron-sulfur cluster assembly protein hIscA1. (PMID: 20302570) Lu J. … Ding H. (Biochem. J. 2010) 23 67
  4. Clinical manifestation and a new ISCU mutation in iron-sulphur cluster deficiency myopathy. (PMID: 19567699) Kollberg G. … Holme E. (Brain 2009) 23 67
  5. The iron-sulfur clusters of dehydratases are primary intracellular targets of copper toxicity. (PMID: 19416816) Macomber L. … Imlay J.A. (Proc. Natl. Acad. Sci. U.S.A. 2009) 23 67

Products for ISCU Gene

Sources for ISCU Gene

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