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Aliases for EPHX2 Gene

Aliases for EPHX2 Gene

  • Epoxide Hydrolase 2 2 3
  • Epoxide Hydrolase 2, Cytoplasmic 2 3 5
  • Epoxide Hydrolase 2, Cytosolic 3
  • Epoxide Hydrolase, Soluble 3
  • Epoxide Hydratase 3
  • CEH 3
  • SEH 3

External Ids for EPHX2 Gene

Previous GeneCards Identifiers for EPHX2 Gene

  • GC08P027131
  • GC08P027853
  • GC08P027370
  • GC08P027404
  • GC08P025893
  • GC08P027348

Summaries for EPHX2 Gene

Entrez Gene Summary for EPHX2 Gene

  • This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012]

GeneCards Summary for EPHX2 Gene

EPHX2 (Epoxide Hydrolase 2) is a Protein Coding gene. Diseases associated with EPHX2 include Hypercholesterolemia, Familial and Fetal Hydantoin Syndrome. Among its related pathways are Arachidonic acid metabolism and Metabolism. GO annotations related to this gene include protein homodimerization activity and hydrolase activity. An important paralog of this gene is EPHX3.

UniProtKB/Swiss-Prot for EPHX2 Gene

  • Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro-9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy-octadec-9Z-enoic acid, 12-phosphonooxy-octadec-9E-enoic acid, and p-nitrophenyl phospate.

Gene Wiki entry for EPHX2 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for EPHX2 Gene

Genomics for EPHX2 Gene

Regulatory Elements for EPHX2 Gene

Enhancers for EPHX2 Gene
GeneHancer Identifier Score Enhancer Sources TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Other Gene Targets for Enhancer

Enhancers around EPHX2 on UCSC Golden Path with GeneCards custom track

Promoters for EPHX2 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around EPHX2 on UCSC Golden Path with GeneCards custom track

Genomic Location for EPHX2 Gene

Chromosome:
8
Start:
27,490,779 bp from pter
End:
27,545,564 bp from pter
Size:
54,786 bases
Orientation:
Plus strand

Genomic View for EPHX2 Gene

Genes around EPHX2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
EPHX2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for EPHX2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for EPHX2 Gene

Proteins for EPHX2 Gene

  • Protein details for EPHX2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P34913-HYES_HUMAN
    Recommended name:
    Bifunctional epoxide hydrolase 2
    Protein Accession:
    P34913
    Secondary Accessions:
    • B2Z3B1
    • B3KTU8
    • B3KUA0
    • G3V134
    • J3KPH7
    • Q16764
    • Q9HBJ1
    • Q9HBJ2

    Protein attributes for EPHX2 Gene

    Size:
    555 amino acids
    Molecular mass:
    62616 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Homodimer.

    Three dimensional structures from OCA and Proteopedia for EPHX2 Gene

    Alternative splice isoforms for EPHX2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for EPHX2 Gene

Proteomics data for EPHX2 Gene at MOPED

Post-translational modifications for EPHX2 Gene

  • The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation (Probable).
  • The N-terminus is blocked.
  • Ubiquitination at Lys 43, Lys 84, Lys 89, Lys 174, Lys 191, Lys 245, Lys 406, Lys 421, Lys 456, Lys 474, and Lys 530
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for EPHX2 Gene

Domains & Families for EPHX2 Gene

Gene Families for EPHX2 Gene

Suggested Antigen Peptide Sequences for EPHX2 Gene

Graphical View of Domain Structure for InterPro Entry

P34913

UniProtKB/Swiss-Prot:

HYES_HUMAN :
  • The N-terminal domain has phosphatase activity. The C-terminal domain has epoxide hydrolase activity.
  • Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.
Domain:
  • The N-terminal domain has phosphatase activity. The C-terminal domain has epoxide hydrolase activity.
Family:
  • Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.
genes like me logo Genes that share domains with EPHX2: view

Function for EPHX2 Gene

Molecular function for EPHX2 Gene

GENATLAS Biochemistry:
epoxyde hydrolase,soluble
UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=21 uM for threo-9,10-phosphonooxy-hydroxy-octadecanoic acid {ECO:0000269 PubMed:12574508, ECO:0000269 PubMed:12574510, ECO:0000269 PubMed:15196990}; KM=1.1 mM for p-nitrophenyl phosphate {ECO:0000269 PubMed:12574508, ECO:0000269 PubMed:12574510, ECO:0000269 PubMed:15196990}; Vmax=338 nmol/min/mg enzyme with threo-9,10-phosphonooxy- hydroxy-octadecanoic acid {ECO:0000269 PubMed:12574508, ECO:0000269 PubMed:12574510, ECO:0000269 PubMed:15196990}; Vmax=5.8 nmol/min/mg enzyme with p-nitrophenyl phosphate {ECO:0000269 PubMed:12574508, ECO:0000269 PubMed:12574510, ECO:0000269 PubMed:15196990};
UniProtKB/Swiss-Prot CatalyticActivity:
An epoxide + H(2)O = a glycol.
UniProtKB/Swiss-Prot CatalyticActivity:
(9S,10S)-10-hydroxy-9-(phosphonooxy)octadecanoate + H(2)O = (9S,10S)-9,10-dihydroxyoctadecanoate + phosphate.
UniProtKB/Swiss-Prot Function:
Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro-9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy-octadec-9Z-enoic acid, 12-phosphonooxy-octadec-9E-enoic acid, and p-nitrophenyl phospate.
UniProtKB/Swiss-Prot Induction:
By compounds that cause peroxisome proliferation such as clofibrate, tiadenol and fenofibrate.

Gene Ontology (GO) - Molecular Function for EPHX2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004301 epoxide hydrolase activity TAS --
GO:0015643 toxic substance binding IDA 15096040
GO:0016787 hydrolase activity IEA --
GO:0042577 lipid phosphatase activity IDA 12574510
genes like me logo Genes that share ontologies with EPHX2: view
genes like me logo Genes that share phenotypes with EPHX2: view

Human Phenotype Ontology for EPHX2 Gene

HPO Id HPO Name Alternative Ids Definition Synonyms

Animal Models for EPHX2 Gene

MGI Knock Outs for EPHX2:

Animal Model Products

  • Taconic Biosciences Mouse Models for EPHX2

CRISPR Products

No data available for Enzyme Numbers (IUBMB) , Transcription Factor Targets and HOMER Transcription for EPHX2 Gene

Localization for EPHX2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for EPHX2 Gene

Cytoplasm. Peroxisome.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for EPHX2 Gene COMPARTMENTS Subcellular localization image for EPHX2 gene
Compartment Confidence
cytosol 5
extracellular 5
peroxisome 5
endoplasmic reticulum 2
plasma membrane 2
lysosome 1
nucleus 1
vacuole 1

Gene Ontology (GO) - Cellular Components for EPHX2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IDA --
GO:0005777 peroxisome IDA 16314446
genes like me logo Genes that share ontologies with EPHX2: view

Pathways & Interactions for EPHX2 Gene

genes like me logo Genes that share pathways with EPHX2: view

Interacting Proteins for EPHX2 Gene

Gene Ontology (GO) - Biological Process for EPHX2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006805 xenobiotic metabolic process NAS 10862610
GO:0006874 cellular calcium ion homeostasis NAS 10862610
GO:0006954 inflammatory response NAS 10862610
GO:0009636 response to toxic substance NAS 10862610
GO:0016311 dephosphorylation IDA 12574508
genes like me logo Genes that share ontologies with EPHX2: view

No data available for SIGNOR curated interactions for EPHX2 Gene

Drugs & Compounds for EPHX2 Gene

(41) Drugs for EPHX2 Gene - From: DrugBank, DGIdb, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
4-{[(CYCLOHEXYLAMINO)CARBONYL]AMINO}BUTANOIC ACID Experimental Pharma Target 0
6-{[(CYCLOHEXYLAMINO)CARBONYL]AMINO}HEXANOIC ACID Experimental Pharma Target 0
7-{[(CYCLOHEXYLAMINO)CARBONYL]AMINO}HEPTANOIC ACID Experimental Pharma Target 0
N-[(CYCLOHEXYLAMINO)CARBONYL]GLYCINE Experimental Pharma Target 0
N-Cyclohexyl-N'-(4-Iodophenyl)Urea Experimental Pharma Target 0

(64) Additional Compounds for EPHX2 Gene - From: Novoseek and HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
(9S,10S)-10-hydroxy-9-(phosphonooxy)octadecanoate
(9S,10S)-9,10-dihydroxyoctadecanoate
11,12-DiHETrE
  • (+/-)-11,12-dihydroxy-5Z,8Z,14Z,17Z-eicosatetraenoate
  • (+/-)-11,12-dihydroxy-5Z,8Z,14Z,17Z-eicosatetraenoic acid
  • (5Z,8Z,14Z)-11,12-Dihydroxyeicosa-5,8,14-trienoate
  • (5Z,8Z,14Z)-11,12-Dihydroxyeicosa-5,8,14-trienoic acid
  • (5Z,8Z,14Z)-11,12-Dihydroxyicosa-5,8,14-trienoate
192461-95-3
11,12-EpETrE
  • (+/-)11(12)-EET
  • (+/-)11(12)-EpETrE
  • (+/-)11,12-EpETrE
  • (5Z,8Z,14Z)-11,12-Epoxyeicosa-5,8,14-trienoate
  • (5Z,8Z,14Z)-11,12-Epoxyeicosa-5,8,14-trienoic acid
11,12-Epoxyeicosatrienoic acid
  • (5Z,8Z,14Z)-11,12-Epoxyeicosa-5,8,14-trienoate
  • (5Z,8Z,14Z)-11,12-Epoxyeicosa-5,8,14-trienoic acid
  • (5Z,8Z,14Z)-11,12-Epoxyicosa-5,8,14-trienoate
  • (5Z,8Z,14Z)-11,12-Epoxyicosa-5,8,14-trienoic acid
  • 10-(3-(2-Octenyl)oxiranyl)-5,8-Decadienoate
81276-02-0
genes like me logo Genes that share compounds with EPHX2: view

Transcripts for EPHX2 Gene

Unigene Clusters for EPHX2 Gene

Epoxide hydrolase 2, cytoplasmic:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for EPHX2 Gene

ExUns: 1 ^ 2a · 2b · 2c · 2d ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b ^ 7a · 7b · 7c · 7d ^ 8 ^ 9a · 9b ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17 ^
SP1: - - -
SP2: - - - - -
SP3: - - - -
SP4: - - - -
SP5: - - -
SP6: -
SP7: -
SP8: - - - -
SP9: - - -
SP10:
SP11:

ExUns: 18 ^ 19 ^ 20 ^ 21
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:

Relevant External Links for EPHX2 Gene

GeneLoc Exon Structure for
EPHX2
ECgene alternative splicing isoforms for
EPHX2

Expression for EPHX2 Gene

mRNA expression in normal human tissues for EPHX2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for EPHX2 Gene

This gene is overexpressed in Liver (x7.9).

Protein differential expression in normal tissues from HIPED for EPHX2 Gene

This gene is overexpressed in Liver, secretome (10.9) and Liver (8.3).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for EPHX2 Gene



SOURCE GeneReport for Unigene cluster for EPHX2 Gene Hs.212088

genes like me logo Genes that share expression patterns with EPHX2: view

Protein tissue co-expression partners for EPHX2 Gene

- Elite partner

Primer Products

No data available for mRNA Expression by UniProt/SwissProt for EPHX2 Gene

Orthologs for EPHX2 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for EPHX2 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia EPHX2 35
  • 83.12 (n)
  • 74.77 (a)
EPHX2 36
  • 75 (a)
OneToOne
dog
(Canis familiaris)
Mammalia EPHX2 35
  • 84.8 (n)
  • 78.56 (a)
EPHX2 36
  • 78 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Ephx2 35
  • 79.08 (n)
  • 73.42 (a)
Ephx2 16
Ephx2 36
  • 73 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia EPHX2 35
  • 99.22 (n)
  • 98.92 (a)
EPHX2 36
  • 99 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Ephx2 35
  • 79.32 (n)
  • 73.24 (a)
oppossum
(Monodelphis domestica)
Mammalia EPHX2 36
  • 61 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia EPHX2 36
  • 59 (a)
OneToOne
chicken
(Gallus gallus)
Aves EPHX2 35
  • 62.93 (n)
  • 57.35 (a)
EPHX2 36
  • 55 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia EPHX2 36
  • 47 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia ephx2 35
  • 62.07 (n)
  • 57.53 (a)
Str.6967 35
African clawed frog
(Xenopus laevis)
Amphibia Xl.18049 35
zebrafish
(Danio rerio)
Actinopterygii ephx2 35
  • 56.22 (n)
  • 50.75 (a)
ephx2 36
  • 48 (a)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea ceeh-1 36
  • 23 (a)
ManyToMany
ceeh-2 36
  • 25 (a)
ManyToMany
thale cress
(Arabidopsis thaliana)
eudicotyledons AT3G51000 35
  • 47.6 (n)
  • 38.13 (a)
rice
(Oryza sativa)
Liliopsida Os12g0636400 35
  • 47.17 (n)
  • 35.64 (a)
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.1281 35
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 25 (a)
ManyToMany
Species with no ortholog for EPHX2:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for EPHX2 Gene

ENSEMBL:
Gene Tree for EPHX2 (if available)
TreeFam:
Gene Tree for EPHX2 (if available)

Paralogs for EPHX2 Gene

Paralogs for EPHX2 Gene

(1) SIMAP similar genes for EPHX2 Gene using alignment to 5 proteins:

genes like me logo Genes that share paralogs with EPHX2: view

Variants for EPHX2 Gene

Sequence variations from dbSNP and Humsavar for EPHX2 Gene

SNP ID Clin Chr 08 pos Sequence Context AA Info Type
rs751141 - 27,516,348(-) GGACC(C/T)GGTAA reference, missense
rs17057255 - 27,503,724(+) TCAAC(C/T)GCCCC reference, missense
rs41507953 - 27,500,988(+) TATGA(A/G)AGGAG intron-variant, reference, missense
VAR_055392 -
rs72475803 - 27,500,979(+) TACCC(A/G)GCTTA intron-variant, reference, missense, utr-variant-5-prime

Structural Variations from Database of Genomic Variants (DGV) for EPHX2 Gene

Variant ID Type Subtype PubMed ID
nsv831275 CNV Loss 17160897
nsv890696 CNV Gain 21882294
esv28788 CNV Loss 19812545
nsv890697 CNV Loss 21882294
nsv437612 CNV Loss 16327808

Variation tolerance for EPHX2 Gene

Residual Variation Intolerance Score: 98% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 8.72; 86.58% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for EPHX2 Gene

Human Gene Mutation Database (HGMD)
EPHX2

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for EPHX2 Gene

Disorders for EPHX2 Gene

MalaCards: The human disease database

(5) MalaCards diseases for EPHX2 Gene - From: OMIM, ClinVar, DISEASES, and Novoseek

Disorder Aliases PubMed IDs
hypercholesterolemia, familial
  • hypercholesterolemia, familial, modifier of
fetal hydantoin syndrome
  • dilantin embryopathy
staphyloenterotoxemia
  • staphylococcal food poisoning
reticulohistiocytic granuloma
  • reticulohistiocytoma
multicentric reticulohistiocytosis
  • lipoid dermatoarthritis
- elite association - COSMIC cancer census association via MalaCards
Search EPHX2 in MalaCards View complete list of genes associated with diseases

Relevant External Links for EPHX2

Genetic Association Database (GAD)
EPHX2
Human Genome Epidemiology (HuGE) Navigator
EPHX2
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
EPHX2
genes like me logo Genes that share disorders with EPHX2: view

No data available for UniProtKB/Swiss-Prot and Genatlas for EPHX2 Gene

Publications for EPHX2 Gene

  1. Genetic variation in soluble epoxide hydrolase (EPHX2) and risk of coronary heart disease: The Atherosclerosis Risk in Communities (ARIC) study. (PMID: 16595607) Lee C.R. … Zeldin D.C. (Hum. Mol. Genet. 2006) 3 23 48 67
  2. Homozygosity for the EPHX2 K55R polymorphism increases the long-term risk of ischemic stroke in men: a study in Swedes. (PMID: 20065888) Fava C. … Melander O. (Pharmacogenet. Genomics 2010) 3 23
  3. Inhibition of the soluble epoxide hydrolase by tyrosine nitration. (PMID: 19704161) Barbosa-Sicard E. … Fleming I. (J. Biol. Chem. 2009) 3 23
  4. Transcriptional regulation of the human soluble epoxide hydrolase gene EPHX2. (PMID: 18078836) Tanaka H. … Hammock B.D. (Biochim. Biophys. Acta 2008) 3 23
  5. Distribution and expression of soluble epoxide hydrolase in human brain. (PMID: 18319271) Sura P. … Grant D.F. (J. Histochem. Cytochem. 2008) 3 23

Products for EPHX2 Gene

Sources for EPHX2 Gene

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