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Aliases for EPHB4 Gene

Aliases for EPHB4 Gene

  • EPH Receptor B4 2 3
  • MYK1 3 4 6
  • HTK 3 4 6
  • Tyrosine-Protein Kinase TYRO11 3 4
  • Hepatoma Transmembrane Kinase 3 4
  • EC 2.7.10.1 4 63
  • TYRO11 3 4
  • Tyrosine-Protein Kinase Receptor HTK 3
  • Ephrin Type-B Receptor 4 3
  • Soluble EPHB4 Variant 1 3
  • Soluble EPHB4 Variant 2 3
  • Soluble EPHB4 Variant 3 3
  • Ephrin Receptor EphB4 3
  • EC 2.7.10 63
  • EphB4 2

External Ids for EPHB4 Gene

Previous HGNC Symbols for EPHB4 Gene

  • HTK

Previous GeneCards Identifiers for EPHB4 Gene

  • GC07M098934
  • GC07M099997
  • GC07M100011
  • GC07M100044
  • GC07M100238
  • GC07M100402
  • GC07M095030

Summaries for EPHB4 Gene

Entrez Gene Summary for EPHB4 Gene

  • Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development. [provided by RefSeq, Jul 2008]

GeneCards Summary for EPHB4 Gene

EPHB4 (EPH Receptor B4) is a Protein Coding gene. Diseases associated with EPHB4 include prostate cancer and breast cancer. Among its related pathways are GPCR Pathway and Akt Signaling. GO annotations related to this gene include transmembrane receptor protein tyrosine kinase activity and ephrin receptor activity. An important paralog of this gene is EPHB3.

UniProtKB/Swiss-Prot for EPHB4 Gene

  • Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis.

Tocris Summary for EPHB4 Gene

  • Ephrins are a family of receptor tyrosine kinases (RTKs) that influence cell migration. In conjunction with the ephrin ligands, ephrin receptors are involved in a variety of developmental events. EphB4 is overexpressed in many tumor types, including breast and prostate cancer.

Gene Wiki entry for EPHB4 Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for EPHB4 Gene

Genomics for EPHB4 Gene

Regulatory Elements for EPHB4 Gene

Genomic Location for EPHB4 Gene

Start:
100,802,565 bp from pter
End:
100,827,521 bp from pter
Size:
24,957 bases
Orientation:
Minus strand

Genomic View for EPHB4 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for EPHB4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for EPHB4 Gene

Proteins for EPHB4 Gene

  • Protein details for EPHB4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P54760-EPHB4_HUMAN
    Recommended name:
    Ephrin type-B receptor 4
    Protein Accession:
    P54760
    Secondary Accessions:
    • B5A970
    • B5A971
    • B5A972
    • Q7Z635
    • Q9BTA5
    • Q9BXP0

    Protein attributes for EPHB4 Gene

    Size:
    987 amino acids
    Molecular mass:
    108270 Da
    Quaternary structure:
    • Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity).

    Three dimensional structures from OCA and Proteopedia for EPHB4 Gene

    Alternative splice isoforms for EPHB4 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for EPHB4 Gene

Proteomics data for EPHB4 Gene at MOPED

Post-translational modifications for EPHB4 Gene

  • Phosphorylated; autophosphorylation is stimulated by EFNB2
  • Glycosylation at Asn203, Asn335, and Asn426
  • Modification sites at PhosphoSitePlus

Other Protein References for EPHB4 Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains for EPHB4 Gene

Gene Families for EPHB4 Gene

HGNC:
  • EPH :EPH receptors
  • FN3 :Fibronectin type III domain containing
  • SAMD :Sterile alpha motif (SAM) domain containing
IUPHAR :

Graphical View of Domain Structure for InterPro Entry

P54760

UniProtKB/Swiss-Prot:

EPHB4_HUMAN :
  • P54760
Domain:
  • Contains 1 Eph LBD (Eph ligand-binding) domain.
  • Contains 2 fibronectin type-III domains.
  • Contains 1 protein kinase domain.
  • Contains 1 SAM (sterile alpha motif) domain.
Family:
  • Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
genes like me logo Genes that share domains with EPHB4: view

Function for EPHB4 Gene

Molecular function for EPHB4 Gene

GENATLAS Biochemistry: EPH-related tyrosine kinase receptor,binding ephrin B4,expressed in projecting neurons and their target fields,involved in short-range contact-mediated axonal guidance,also involved in angiogenesis by marking venous but not arterial endothelial cells
UniProtKB/Swiss-Prot CatalyticActivity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
UniProtKB/Swiss-Prot Function: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis.

Enzyme Numbers (IUBMB) for EPHB4 Gene

Gene Ontology (GO) - Molecular Function for EPHB4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity --
GO:0004713 protein tyrosine kinase activity --
GO:0004714 transmembrane receptor protein tyrosine kinase activity IDA 8188704
GO:0005003 ephrin receptor activity IDA 12734395
GO:0005515 protein binding IPI 16867992
genes like me logo Genes that share ontologies with EPHB4: view
genes like me logo Genes that share phenotypes with EPHB4: view

miRNA for EPHB4 Gene

No data available for Animal Models , Transcription Factor Targeting and HOMER Transcription for EPHB4 Gene

Localization for EPHB4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for EPHB4 Gene

Cell membrane; Single-pass type I membrane protein.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for EPHB4 Gene COMPARTMENTS Subcellular localization image for EPHB4 gene
Compartment Confidence
plasma membrane 5
extracellular 2
cytoskeleton 1
cytosol 1
lysosome 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for EPHB4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005576 extracellular region TAS --
GO:0005829 cytosol TAS --
GO:0005886 plasma membrane TAS --
GO:0005887 integral component of plasma membrane IDA 8188704
GO:0070062 extracellular exosome IDA 23376485
genes like me logo Genes that share ontologies with EPHB4: view

Pathways for EPHB4 Gene

genes like me logo Genes that share pathways with EPHB4: view

Pathways by source for EPHB4 Gene

Gene Ontology (GO) - Biological Process for EPHB4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001525 angiogenesis ISS --
GO:0002042 cell migration involved in sprouting angiogenesis IDA 12734395
GO:0003007 heart morphogenesis ISS --
GO:0006468 protein phosphorylation --
GO:0007155 cell adhesion IDA 12734395
genes like me logo Genes that share ontologies with EPHB4: view

Compounds for EPHB4 Gene

(5) Tocris Compounds for EPHB4 Gene

Compound Action Cas Number
DDR1-IN-1 dihydrochloride Selective DDR1 inhibitor
GTP 14564 Class III receptor tyrosine kinase (RTK) inhibitor [34823-86-4]
Ki 20227 c-Fms inhibitor; suppresses osteolysis and osteoclast accumulation [623142-96-1]
KYL EphA4 receptor tyrosine kinase antagonist; neuroprotective [676657-00-4]
NVP BHG 712 Potent EphB4 kinase inhibitor [940310-85-0]

(2) HMDB Compounds for EPHB4 Gene

Compound Synonyms Cas Number PubMed IDs
Adenosine triphosphate
  • 5'-(tetrahydrogen triphosphate) Adenosine
56-65-5
ADP
  • adenosindiphosphorsaeure
58-64-0

(3) Novoseek inferred chemical compound relationships for EPHB4 Gene

Compound -log(P) Hits PubMed IDs
tyrosine 66 25
vegf 40.8 8
thymidine 38.9 7
genes like me logo Genes that share compounds with EPHB4: view

Transcripts for EPHB4 Gene

Unigene Clusters for EPHB4 Gene

EPH receptor B4:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for EPHB4 Gene

ExUns: 1a · 1b ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9a · 9b ^ 10 ^ 11a · 11b · 11c ^ 12a · 12b ^ 13 ^ 14a · 14b ^ 15 ^ 16 ^ 17
SP1: - - -
SP2: - - - -
SP3: - -
SP4: -
SP5: -
SP6:

Relevant External Links for EPHB4 Gene

GeneLoc Exon Structure for
EPHB4
ECgene alternative splicing isoforms for
EPHB4

Expression for EPHB4 Gene

mRNA expression in normal human tissues for EPHB4 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for EPHB4 Gene

SOURCE GeneReport for Unigene cluster for EPHB4 Gene Hs.437008

mRNA Expression by UniProt/SwissProt for EPHB4 Gene

P54760-EPHB4_HUMAN
Tissue specificity: Abundantly expressed in placenta but also detected in kidney, liver, lung, pancreas, skeletal muscle and heart. Expressed in primitive and myeloid, but not lymphoid, hematopoietic cells. Also observed in cell lines derived from liver, breast, colon, lung, melanocyte and cervix.
genes like me logo Genes that share expressions with EPHB4: view

No data available for mRNA differential expression in normal tissues for EPHB4 Gene

Orthologs for EPHB4 Gene

This gene was present in the common ancestor of animals.

Orthologs for EPHB4 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia EPHB4 35
  • 99.7 (n)
  • 100 (a)
EPHB4 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia EPHB4 35
  • 90.68 (n)
  • 96.05 (a)
EPHB4 36
  • 96 (a)
OneToOne
dog
(Canis familiaris)
Mammalia EPHB4 35
  • 89.36 (n)
  • 96.86 (a)
EPHB4 36
  • 97 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Ephb4 35
  • 86.47 (n)
  • 93.11 (a)
Ephb4 16
Ephb4 36
  • 92 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia EPHB4 36
  • 89 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia EPHB4 36
  • 88 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Ephb4 35
  • 86.16 (n)
  • 93.32 (a)
African clawed frog
(Xenopus laevis)
Amphibia ephB4a 35
tropical clawed frog
(Silurana tropicalis)
Amphibia Str.3205 35
zebrafish
(Danio rerio)
Actinopterygii -- 35
ephb4a 35
  • 64.1 (n)
  • 64.06 (a)
ephb4a 36
  • 62 (a)
OneToMany
ephb4b 36
  • 60 (a)
OneToMany
fruit fly
(Drosophila melanogaster)
Insecta Eph 36
  • 36 (a)
OneToMany
Eph 37
  • 38 (a)
hop 37
  • 35 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 42 (a)
ManyToMany
-- 36
  • 39 (a)
ManyToMany
CSA.1664 36
  • 38 (a)
ManyToMany
CSA.7809 36
  • 37 (a)
ManyToMany
Species with no ortholog for EPHB4:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for EPHB4 Gene

ENSEMBL:
Gene Tree for EPHB4 (if available)
TreeFam:
Gene Tree for EPHB4 (if available)

Paralogs for EPHB4 Gene

Paralogs for EPHB4 Gene

genes like me logo Genes that share paralogs with EPHB4: view

Variants for EPHB4 Gene

Sequence variations from dbSNP and Humsavar for EPHB4 Gene

SNP ID Clin Chr 07 pos Sequence Context AA Info Type MAF
rs144173 -- 100,818,628(-) GTGTC(C/T)GACAT synonymous-codon, reference
rs221005 -- 100,803,811(-) GGCCC(C/T)GCCTC intron-variant
rs314307 -- 100,821,606(-) ctgtc(A/G)cccag intron-variant
rs314308 -- 100,823,256(-) TCTTC(A/C/G)GAGTT intron-variant
rs314309 -- 100,823,322(-) AGCTC(A/G)AGACT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for EPHB4 Gene

Variant ID Type Subtype PubMed ID
esv2734888 CNV Deletion 23290073
nsv470381 CNV Loss 18288195
nsv888796 CNV Loss 21882294
dgv2119e1 CNV Complex 17122850
dgv7402n71 CNV Loss 21882294
dgv7403n71 CNV Loss 21882294
nsv888800 CNV Loss 21882294

Relevant External Links for EPHB4 Gene

HapMap Linkage Disequilibrium report
EPHB4

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for EPHB4 Gene

Disorders for EPHB4 Gene

MalaCards: The human disease database

MalaCards: The human disease database. (2) Diseases for EPHB4 Gene including...

Search for EPHB4 Gene in MalaCards »

(1) University of Copenhagen DISEASES for EPHB4 Gene

(22) Novoseek inferred disease relationships for EPHB4 Gene

Disease -log(P) Hits PubMed IDs
retinal neovascularization 61.7 1
neovascularization 52.9 2
hnscc 47 14
cancer 32.9 11
proliferative diabetic retinopathy 31.3 1

Relevant External Links for EPHB4

Genetic Association Database (GAD)
EPHB4
Human Genome Epidemiology (HuGE) Navigator
EPHB4
genes like me logo Genes that share disorders with EPHB4: view

No data available for UniProtKB/Swiss-Prot for EPHB4 Gene

Publications for EPHB4 Gene

  1. Cloning and characterization of HTK, a novel transmembrane tyrosine kinase of the EPH subfamily. (PMID: 8188704) Bennett B.D. … Scadden D.T. (J. Biol. Chem. 1994) 2 3 4 23
  2. EphB4 controls blood vascular morphogenesis during postnatal angiogenesis. (PMID: 16424904) Erber R. … Vajkoczy P. (EMBO J. 2006) 3 4 23
  3. Structural and biophysical characterization of the EphB4*ephrinB2 protein-protein interaction and receptor specificity. (PMID: 16867992) Chrencik J.E. … Kuhn P. (J. Biol. Chem. 2006) 3 4 23
  4. Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells. (PMID: 12734395) Fueller T. … Augustin H.G. (J. Cell Sci. 2003) 3 4 23
  5. Eph B4 receptor signaling mediates endothelial cell migration and proliferation via the phosphatidylinositol 3-kinase pathway. (PMID: 12235151) Steinle J.J. … Granger H.J. (J. Biol. Chem. 2002) 3 23

Products for EPHB4 Gene

  • Addgene plasmids for EPHB4

Sources for EPHB4 Gene

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