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Aliases for E4F1 Gene

Aliases for E4F1 Gene

  • E4F Transcription Factor 1 2 3 4 5
  • Putative E3 Ubiquitin-Protein Ligase E4F1 3 4
  • RING-Type E3 Ubiquitin Transferase E4F1 3 4
  • Transcription Factor E4F 3 4
  • P120E4F 3 4
  • P50E4F 3 4
  • E4F 3 4
  • Transcription Factor E4F1 3
  • EC 2.3.2.27 4

External Ids for E4F1 Gene

Previous GeneCards Identifiers for E4F1 Gene

  • GC16M002500
  • GC16P002299
  • GC16P002213
  • GC16P002274
  • GC16P002199
  • GC16P002275
  • GC16P002276

Summaries for E4F1 Gene

Entrez Gene Summary for E4F1 Gene

  • The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence. Alternative splicing results in multiple transcripts encoding different proteins. [provided by RefSeq, Jan 2014]

GeneCards Summary for E4F1 Gene

E4F1 (E4F Transcription Factor 1) is a Protein Coding gene. Among its related pathways are p53 pathway (Pathway Interaction Database). GO annotations related to this gene include nucleic acid binding and ligase activity.

UniProtKB/Swiss-Prot for E4F1 Gene

  • May function as a transcriptional repressor. May also function as a ubiquitin ligase mediating ubiquitination of chromatin-associated TP53. Functions in cell survival and proliferation through control of the cell cycle. Functions in the p53 and pRB tumor suppressor pathways and regulates the cyclin CCNA2 transcription.

  • Identified as a cellular target of the adenoviral oncoprotein E1A, it is required for both transcriptional activation and repression of viral genes.

Gene Wiki entry for E4F1 Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for E4F1 Gene

Genomics for E4F1 Gene

Regulatory Elements for E4F1 Gene

Enhancers for E4F1 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH16G003156 1.7 FANTOM5 ENCODE dbSUPER 5.1 +935.3 935306 3.8 CREB3L1 DMAP1 YY1 ZNF263 SP3 NFYC TBX21 MEF2D SSRP1 ZNF610 ZNF213-AS1 ZNF205 CLUAP1 RNPS1 MTCO1P28 ZNF174 ZNF263 MTND1P8 TIGD7 MTRNR2L4
GH16G002467 1.7 FANTOM5 ENCODE dbSUPER 2.4 +246.2 246207 5.4 HDGF PKNOX1 AGO1 ARID4B SIN3A FEZF1 DMAP1 YBX1 ZNF2 YY1 ERVK13-1 NME3 ENSG00000260646 MTCO1P28 SNORD60 SNHG19 ZNF205 ENSG00000261123 NTN3 RAB26
GH16G002529 1.5 FANTOM5 ENCODE 1.4 +308.4 308359 3.8 HDGF HNRNPUL1 AGO1 ARID4B SIN3A FEZF1 DMAP1 ZNF2 YY1 ZNF143 TBC1D24 LOC652276 ATP6V0C CEMP1 PDPK1 AMDHD2 SRRM2 E4F1 RAB26 GC16P002898
GH16G002222 1.1 ENCODE 0.7 +0.7 686 2.7 HDGF PKNOX1 CREB3L1 AGO1 ZFP64 ARID4B SIN3A DMAP1 ZNF2 YY1 ZNF598 ENSG00000261505 TRAF7 ENSG00000260051 ENSG00000260954 PGP ENSG00000255513 THOC6 SNORA64 LOC105371055
GH16G002226 0.9 ENCODE 0.7 +3.1 3126 1.1 ELF3 FOXA2 ARID4B THRB ZSCAN9 RARA ZNF121 CREM THAP11 PAF1 E4F1 DNASE1L2
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around E4F1 on UCSC Golden Path with GeneCards custom track

Promoters for E4F1 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000082457 312 1601 HDGF PKNOX1 AGO1 ZFP64 ARID4B SIN3A DMAP1 ZNF2 YY1 SLC30A9

Genomic Location for E4F1 Gene

Chromosome:
16
Start:
2,223,488 bp from pter
End:
2,235,742 bp from pter
Size:
12,255 bases
Orientation:
Plus strand

Genomic View for E4F1 Gene

Genes around E4F1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
E4F1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for E4F1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for E4F1 Gene

Proteins for E4F1 Gene

  • Protein details for E4F1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q66K89-E4F1_HUMAN
    Recommended name:
    Transcription factor E4F1
    Protein Accession:
    Q66K89
    Secondary Accessions:
    • A8K2R4
    • O00146

    Protein attributes for E4F1 Gene

    Size:
    784 amino acids
    Molecular mass:
    83496 Da
    Quaternary structure:
    • Homodimer; binds DNA as a dimer. Forms a complex with CDKN2A and TP53. Interactions with TP53, RB1, ANP32A, BMI1 and FHL2 regulate E4F1 activity. Interacts with HDAC1, HMGA2 and RASSF1. Interacts with HBV protein X.

neXtProt entry for E4F1 Gene

Post-translational modifications for E4F1 Gene

  • May be sumoylated by UBE2I upon interaction with CDKN2A.
  • Phosphorylated; p120E4F and p50E4F are both phosphorylated. Phosphorylation is cell cycle-dependent and differentially regulates DNA-binding activity and function of both forms.
  • Proteolytic cleavage produces a 50 kDa N-terminal peptide (p50E4F) which has a DNA-binding activity and activates transcription in presence of the adenoviral E1A protein. The major full length protein (p120E4F) functions as a repressor of transcription.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for E4F1 Gene

Domains & Families for E4F1 Gene

Gene Families for E4F1 Gene

Protein Domains for E4F1 Gene

Suggested Antigen Peptide Sequences for E4F1 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

genes like me logo Genes that share domains with E4F1: view

No data available for UniProtKB/Swiss-Prot for E4F1 Gene

Function for E4F1 Gene

Molecular function for E4F1 Gene

GENATLAS Biochemistry:
transcription factor E4F1,adenovirus EIA regulated,with low spliced isoforms,one 120kDa) upregulated,50kDa,down-regulated
UniProtKB/Swiss-Prot CatalyticActivity:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.
UniProtKB/Swiss-Prot Function:
May function as a transcriptional repressor. May also function as a ubiquitin ligase mediating ubiquitination of chromatin-associated TP53. Functions in cell survival and proliferation through control of the cell cycle. Functions in the p53 and pRB tumor suppressor pathways and regulates the cyclin CCNA2 transcription.
UniProtKB/Swiss-Prot Function:
Identified as a cellular target of the adenoviral oncoprotein E1A, it is required for both transcriptional activation and repression of viral genes.
UniProtKB/Swiss-Prot Induction:
Up-regulated by estrogen.

Enzyme Numbers (IUBMB) for E4F1 Gene

Gene Ontology (GO) - Molecular Function for E4F1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000977 RNA polymerase II regulatory region sequence-specific DNA binding IDA 2169022
GO:0001227 transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding IC --
GO:0003676 nucleic acid binding IEA --
GO:0003677 DNA binding TAS 10869426
GO:0003700 transcription factor activity, sequence-specific DNA binding TAS 9121437
genes like me logo Genes that share ontologies with E4F1: view
genes like me logo Genes that share phenotypes with E4F1: view

Animal Models for E4F1 Gene

MGI Knock Outs for E4F1:

Animal Model Products

CRISPR Products

miRNA for E4F1 Gene

miRTarBase miRNAs that target E4F1

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for E4F1 Gene

Localization for E4F1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for E4F1 Gene

Nucleus, nucleoplasm. Cytoplasm. Note=A small fraction is detected in the cytoplasm. Excluded from the nucleolus where it is targeted upon CDKN2A overexpression. Localizes to the mitotic spindle during embryogenesis. {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for E4F1 gene
Compartment Confidence
nucleus 5
cytoskeleton 2
mitochondrion 2

Gene Ontology (GO) - Cellular Components for E4F1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005654 nucleoplasm IDA --
GO:0005737 cytoplasm IEA --
GO:0005819 spindle IEA --
genes like me logo Genes that share ontologies with E4F1: view

Pathways & Interactions for E4F1 Gene

SuperPathway Contained pathways
1 p53 pathway (Pathway Interaction Database)
genes like me logo Genes that share pathways with E4F1: view

Pathways by source for E4F1 Gene

1 BioSystems pathway for E4F1 Gene

UniProtKB/Swiss-Prot Q66K89-E4F1_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

SIGNOR curated interactions for E4F1 Gene

Is inactivated by:

Gene Ontology (GO) - Biological Process for E4F1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000122 negative regulation of transcription from RNA polymerase II promoter IDA 14645522
GO:0006260 DNA replication IEA --
GO:0006351 transcription, DNA-templated IEA --
GO:0006355 regulation of transcription, DNA-templated IEA --
GO:0007049 cell cycle IEA --
genes like me logo Genes that share ontologies with E4F1: view

Transcripts for E4F1 Gene

Alternative Splicing Database (ASD) splice patterns (SP) for E4F1 Gene

ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3a · 3b ^ 4 ^ 5a · 5b · 5c ^ 6a · 6b ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12
SP1:
SP2:
SP3: -
SP4:
SP5:
SP6:

Relevant External Links for E4F1 Gene

GeneLoc Exon Structure for
E4F1
ECgene alternative splicing isoforms for
E4F1

Expression for E4F1 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for E4F1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for E4F1 Gene

This gene is overexpressed in CD8 Tcells (54.2) and Placenta (14.8).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for E4F1 Gene



Protein tissue co-expression partners for E4F1 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of E4F1 Gene:

E4F1

SOURCE GeneReport for Unigene cluster for E4F1 Gene:

Hs.513268

mRNA Expression by UniProt/SwissProt for E4F1 Gene:

Q66K89-E4F1_HUMAN
Tissue specificity: Ubiquitously expressed.

Evidence on tissue expression from TISSUES for E4F1 Gene

  • Nervous system(2.3)
genes like me logo Genes that share expression patterns with E4F1: view

Primer Products

No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for E4F1 Gene

Orthologs for E4F1 Gene

This gene was present in the common ancestor of chordates.

Orthologs for E4F1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia E4F1 34 35
  • 98.04 (n)
cow
(Bos Taurus)
Mammalia E4F1 34 35
  • 87.75 (n)
dog
(Canis familiaris)
Mammalia E4F1 34 35
  • 86.72 (n)
mouse
(Mus musculus)
Mammalia E4f1 34 16 35
  • 85.73 (n)
rat
(Rattus norvegicus)
Mammalia E4f1 34
  • 84.72 (n)
oppossum
(Monodelphis domestica)
Mammalia E4F1 35
  • 71 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia E4F1 35
  • 60 (a)
OneToOne
chicken
(Gallus gallus)
Aves E4F1 34 35
  • 68.82 (n)
lizard
(Anolis carolinensis)
Reptilia E4F1 35
  • 62 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia e4f1 34
  • 65.69 (n)
Str.8417 34
zebrafish
(Danio rerio)
Actinopterygii e4f1 34 35
  • 58.26 (n)
Species where no ortholog for E4F1 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for E4F1 Gene

ENSEMBL:
Gene Tree for E4F1 (if available)
TreeFam:
Gene Tree for E4F1 (if available)

Paralogs for E4F1 Gene

genes like me logo Genes that share paralogs with E4F1: view

No data available for Paralogs for E4F1 Gene

Variants for E4F1 Gene

Sequence variations from dbSNP and Humsavar for E4F1 Gene

SNP ID Clin Chr 16 pos Sequence Context AA Info Type
rs1000359585 -- 2,236,089(+) GGGAG(A/T)GTGGC downstream-variant-500B, upstream-variant-2KB
rs1000621430 -- 2,226,800(+) AGTGA(A/G)GGGGT intron-variant
rs1000656598 -- 2,229,598(+) AGAGG(A/G)GCCCA nc-transcript-variant, reference, missense
rs1000704081 -- 2,229,758(+) ATGCT(C/G/T)GTCTC intron-variant
rs1001215834 -- 2,224,634(+) ATACA(A/G)AAAAA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for E4F1 Gene

Variant ID Type Subtype PubMed ID
dgv4859n54 CNV loss 21841781
dgv4861n54 CNV loss 21841781
dgv4863n54 CNV loss 21841781
esv2422427 CNV duplication 17116639
esv2673834 CNV deletion 23128226
esv2750378 CNV deletion 23290073
esv3637631 CNV loss 21293372
nsv1052260 CNV gain 25217958
nsv1711 CNV insertion 18451855
nsv457351 CNV loss 19166990
nsv471072 CNV loss 18288195
nsv571200 CNV loss 21841781
nsv952902 CNV deletion 24416366

Variation tolerance for E4F1 Gene

Residual Variation Intolerance Score: 69.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.73; 46.72% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for E4F1 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
E4F1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for E4F1 Gene

Disorders for E4F1 Gene

Relevant External Links for E4F1

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
E4F1

No disorders were found for E4F1 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for E4F1 Gene

Publications for E4F1 Gene

  1. Interaction of the hepatitis B virus protein HBx with the human transcription regulatory protein p120E4F in vitro. (PMID: 16112766) Rui E. … Kobarg J. (Virus Res. 2006) 3 4 22 64
  2. Identification of the E1A-regulated transcription factor p120 E4F as an interacting partner of the RASSF1A candidate tumor suppressor gene. (PMID: 14729613) Fenton S.L. … Latif F. (Cancer Res. 2004) 3 4 22 64
  3. E4F1, a novel estrogen-responsive gene in possible atheroprotection, revealed by microarray analysis. (PMID: 15579445) Nakamura Y. … Sasano H. (Am. J. Pathol. 2004) 3 4 22 64
  4. Association of p14ARF with the p120E4F transcriptional repressor enhances cell cycle inhibition. (PMID: 12446718) Rizos H. … Kefford R.F. (J. Biol. Chem. 2003) 3 4 22 64
  5. Chromosomal location and tissue expression of the gene encoding the adenovirus E1A-regulated transcription factor E4F in humans and mice. (PMID: 9530632) Rooney R.J. … Copeland N.G. (Mamm. Genome 1998) 2 3 4 64

Products for E4F1 Gene

Sources for E4F1 Gene

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