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Aliases for DAPK3 Gene

Aliases for DAPK3 Gene

  • Death-Associated Protein Kinase 3 2 3 5
  • Zipper-Interacting Protein Kinase 3 4
  • DAP-Like Kinase 3 4
  • DAP Kinase 3 3 4
  • MYPT1 Kinase 3 4
  • EC 2.7.11.1 4 63
  • ZIP-Kinase 3 4
  • ZIPK 3 4
  • DLK 3 4
  • EC 2.7.11 63
  • ZIP 3

External Ids for DAPK3 Gene

Previous GeneCards Identifiers for DAPK3 Gene

  • GC19M004050
  • GC19M003898
  • GC19M003909
  • GC19M003721

Summaries for DAPK3 Gene

Entrez Gene Summary for DAPK3 Gene

  • Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. [provided by RefSeq, Jul 2008]

GeneCards Summary for DAPK3 Gene

DAPK3 (Death-Associated Protein Kinase 3) is a Protein Coding gene. Diseases associated with DAPK3 include recessive dystrophic epidermolysis bullosa. Among its related pathways are Pathways in cancer and Apoptotic cleavage of cellular proteins. GO annotations related to this gene include protein homodimerization activity and transferase activity, transferring phosphorus-containing groups. An important paralog of this gene is MYLK4.

UniProtKB/Swiss-Prot for DAPK3 Gene

  • Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Involved in the regulation of smooth muscle contraction. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in regulation of starvation-induced autophagy. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Overexpression leads to condensation of actin stress fibers into thick bundles. Involved in actin filament focal adhesion dynamics. The function in both reorganization of actin cytoskeleton and focal adhesion dissolution is modulated by RhoD. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Phosphorylates RPL13A on Ser-77 upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Involved in regulation of cell cycle progression and cell proliferation. May be a tumor suppressor.

Gene Wiki entry for DAPK3 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for DAPK3 Gene

Genomics for DAPK3 Gene

Regulatory Elements for DAPK3 Gene

Promoters for DAPK3 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around DAPK3 on UCSC Golden Path with GeneCards custom track

Genomic Location for DAPK3 Gene

Chromosome:
19
Start:
3,958,453 bp from pter
End:
3,971,123 bp from pter
Size:
12,671 bases
Orientation:
Minus strand

Genomic View for DAPK3 Gene

Genes around DAPK3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
DAPK3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for DAPK3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for DAPK3 Gene

Proteins for DAPK3 Gene

  • Protein details for DAPK3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O43293-DAPK3_HUMAN
    Recommended name:
    Death-associated protein kinase 3
    Protein Accession:
    O43293
    Secondary Accessions:
    • A0AVN4
    • B3KQE2
    • Q05JY4

    Protein attributes for DAPK3 Gene

    Size:
    454 amino acids
    Molecular mass:
    52536 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Homooligomer in its kinase-active form (homotrimers and homodimers are reported); monomeric in its kinase-inactive form. Homodimerization is required for activation segment autophosphorylation (Probable). Isoform 1 and isoform 2 interact with myosin and PPP1R12A; interaction of isoform 1 with PPP1R12A is inhibited by RhoA dominant negative form. Interacts with NLK, DAXX, STAT3, RHOD (GTP-bound form) and TCP10L. Interacts with PAWR; the interaction is reported conflictingly: according to PubMed:17953487 does not interact with PAWR. Interacts with ULK1; may be a substrate of ULK1.

    Three dimensional structures from OCA and Proteopedia for DAPK3 Gene

    Alternative splice isoforms for DAPK3 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for DAPK3 Gene

Proteomics data for DAPK3 Gene at MOPED

Post-translational modifications for DAPK3 Gene

  • The phosphorylation status is critical for kinase activity, oligomerization and intracellular localization. Phosphorylation at Thr-180, Thr-225 and Thr-265 is essential for activity. The phosphorylated form is localized in the cytoplasm promoted by phosphorylation at Thr-299; nuclear translocation or retention is maximal when it is not phosphorylated. Phosphorylation increases the trimeric form, and its dephosphorylation favors a kinase-inactive monomeric form. Both isoform 1 and isoform 2 can undergo autophosphorylation.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for DAPK3 Gene

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for DAPK3 (DAPK3)

Domains & Families for DAPK3 Gene

Suggested Antigen Peptide Sequences for DAPK3 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

O43293

UniProtKB/Swiss-Prot:

DAPK3_HUMAN :
  • Contains 1 protein kinase domain.
  • Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
Domain:
  • Contains 1 protein kinase domain.
Family:
  • Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
genes like me logo Genes that share domains with DAPK3: view

Function for DAPK3 Gene

Molecular function for DAPK3 Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=12 uM for myosin (isoform 2) {ECO:0000269 PubMed:17126281}; KM=6.2 uM for myosin (isoform 1) {ECO:0000269 PubMed:17126281}; KM=73 uM for MYL12B (isoform 2) {ECO:0000269 PubMed:17126281}; KM=10.4 uM for MYL12B (isoform 1) {ECO:0000269 PubMed:17126281}; Vmax=248 nmol/min/mg enzyme toward myosin (isoform 2) {ECO:0000269 PubMed:17126281}; Vmax=120 nmol/min/mg enzyme toward myosin (isoform 1) {ECO:0000269 PubMed:17126281}; Vmax=1.3 umol/min/mg enzyme toward MYL12B (isoform 2) {ECO:0000269 PubMed:17126281}; Vmax=271 nmol/min/mg enzyme toward MYL12B (isoform 1) {ECO:0000269 PubMed:17126281};
UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation:
A sequential activation is proposed: autophosphorylation at consensus sites is leading to dimerization of the catalytic domain stabilized by phosphorylation at Ser-50 and activation segment exchange (producing an active confirmation of both kinase modules in trans) followed by phosphorylation at Thr-180 in the activation segment and at other regulatory sites (Probable). Phosphorylation at Thr-180, Thr-225 and Thr-265 is essential for activity. Oligomerization is required for full enzymatic activity. Inhibited by pyridone 6 (K00225), a potent, ATP-competitive inhibitor.
UniProtKB/Swiss-Prot Function:
Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Involved in the regulation of smooth muscle contraction. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in regulation of starvation-induced autophagy. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Overexpression leads to condensation of actin stress fibers into thick bundles. Involved in actin filament focal adhesion dynamics. The function in both reorganization of actin cytoskeleton and focal adhesion dissolution is modulated by RhoD. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Phosphorylates RPL13A on Ser-77 upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Involved in regulation of cell cycle progression and cell proliferation. May be a tumor suppressor.

Enzyme Numbers (IUBMB) for DAPK3 Gene

Gene Ontology (GO) - Molecular Function for DAPK3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0017048 Rho GTPase binding IDA 23454120
GO:0042802 identical protein binding IPI 18239682
genes like me logo Genes that share ontologies with DAPK3: view
genes like me logo Genes that share phenotypes with DAPK3: view

Animal Model Products

miRNA for DAPK3 Gene

miRTarBase miRNAs that target DAPK3

Inhibitory RNA Products

In Situ Assay Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for DAPK3 Gene

Localization for DAPK3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for DAPK3 Gene

Nucleus. Cytoplasm. Note=Predominantly localizes to the cytoplasm but can shuttle between the nucleus and cytoplasm; cytoplasmic localization is promoted by phosphorylation at Thr-299 and involves Rho/Rock signaling. {ECO:0000269 PubMed:17953487, ECO:0000269 PubMed:20854903}.
Isoform 1: Nucleus. Cytoplasm.
Isoform 2: Nucleus. Cytoplasm.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for DAPK3 Gene COMPARTMENTS Subcellular localization image for DAPK3 gene
Compartment Confidence
nucleus 5
cytoskeleton 3
cytosol 3

Gene Ontology (GO) - Cellular Components for DAPK3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005884 actin filament IEA --
genes like me logo Genes that share ontologies with DAPK3: view

Pathways & Interactions for DAPK3 Gene

genes like me logo Genes that share pathways with DAPK3: view

Pathways by source for DAPK3 Gene

2 KEGG pathways for DAPK3 Gene
1 Qiagen pathway for DAPK3 Gene

SIGNOR curated interactions for DAPK3 Gene

Activates:
Is activated by:

Gene Ontology (GO) - Biological Process for DAPK3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000910 cytokinesis TAS --
GO:0006355 regulation of transcription, DNA-templated TAS --
GO:0006468 protein phosphorylation IEA,IDA 10356987
GO:0006915 apoptotic process IMP 12917339
GO:0007088 regulation of mitotic nuclear division TAS --
genes like me logo Genes that share ontologies with DAPK3: view

Drugs & Compounds for DAPK3 Gene

(8) Drugs for DAPK3 Gene - From: HMDB, DrugBank, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Adenosine triphosphate Approved Nutra 0
(4R)-7,8-dichloro-1',9-dimethyl-1-oxo-1,2,4,9-tetrahydrospiro[beta-carboline-3,4'-piperidine]-4-carbonitrile Experimental Pharma Target 0
2-(1,1-DIMETHYLETHYL)9-FLUORO-3,6-DIHYDRO-7H-BENZ[H]-IMIDAZ[4,5-F]ISOQUINOLIN-7-ONE Experimental Pharma Target 0
4-(6-{[(1R)-1-(hydroxymethyl)propyl]amino}imidazo[1,2-b]pyridazin-3-yl)benzoic acid Experimental Pharma Target 0
Magnesium Nutra 0

(1) Additional Compounds for DAPK3 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Full agonist, Agonist 58-64-0
genes like me logo Genes that share compounds with DAPK3: view

Transcripts for DAPK3 Gene

Unigene Clusters for DAPK3 Gene

Death-associated protein kinase 3:
Representative Sequences:

Inhibitory RNA Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for DAPK3 Gene

ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b ^ 8 ^ 9a · 9b
SP1: - -
SP2: - -
SP3:
SP4:

Relevant External Links for DAPK3 Gene

GeneLoc Exon Structure for
DAPK3
ECgene alternative splicing isoforms for
DAPK3

Expression for DAPK3 Gene

mRNA expression in normal human tissues for DAPK3 Gene

Protein differential expression in normal tissues from HIPED for DAPK3 Gene

This gene is overexpressed in Bone marrow mesenchymal stem cell (12.8), CD4 Tcells (7.4), and Gallbladder (7.2).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for DAPK3 Gene



SOURCE GeneReport for Unigene cluster for DAPK3 Gene Hs.631844

mRNA Expression by UniProt/SwissProt for DAPK3 Gene

O43293-DAPK3_HUMAN
Tissue specificity: Widely expressed. Isoform 1 and isoform 2 are expressed in the bladder smooth muscle.
genes like me logo Genes that share expression patterns with DAPK3: view

Primer Products

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and Protein tissue co-expression partners for DAPK3 Gene

Orthologs for DAPK3 Gene

This gene was present in the common ancestor of animals.

Orthologs for DAPK3 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia DAPK3 35
  • 88.47 (n)
  • 94.93 (a)
DAPK3 36
  • 95 (a)
OneToOne
dog
(Canis familiaris)
Mammalia DAPK3 35
  • 89.79 (n)
  • 95.37 (a)
DAPK3 36
  • 95 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Dapk3 35
  • 82.22 (n)
  • 85.04 (a)
Dapk3 16
Dapk3 36
  • 85 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia DAPK3 35
  • 99.41 (n)
  • 100 (a)
DAPK3 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Dapk3 35
  • 81.47 (n)
  • 84.82 (a)
oppossum
(Monodelphis domestica)
Mammalia DAPK3 36
  • 91 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia DAPK3 36
  • 90 (a)
OneToOne
chicken
(Gallus gallus)
Aves DAPK3 35
  • 81.94 (n)
  • 83.8 (a)
DAPK3 36
  • 82 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia dapk3 35
  • 68.82 (n)
  • 79.51 (a)
zebrafish
(Danio rerio)
Actinopterygii dapk3 35
  • 74.92 (n)
  • 82.21 (a)
dapk3 36
  • 81 (a)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea dapk-1 36
  • 12 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 44 (a)
OneToMany
Species with no ortholog for DAPK3:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for DAPK3 Gene

ENSEMBL:
Gene Tree for DAPK3 (if available)
TreeFam:
Gene Tree for DAPK3 (if available)

Paralogs for DAPK3 Gene

Paralogs for DAPK3 Gene

genes like me logo Genes that share paralogs with DAPK3: view

Variants for DAPK3 Gene

Sequence variations from dbSNP and Humsavar for DAPK3 Gene

SNP ID Clin Chr 19 pos Sequence Context AA Info Type
VAR_040438 A colorectal adenocarcinoma sample
VAR_040439 An ovarian mucinous carcinoma sample
VAR_040440 A lung neuroendocrine carcinoma sample
rs2010751 -- 3,968,417(-) ACTCA(C/T)TGCAG intron-variant
rs2278437 -- 3,963,726(+) CCCAG(A/G)ACCGT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for DAPK3 Gene

Variant ID Type Subtype PubMed ID
nsv833715 CNV Loss 17160897
nsv910780 CNV Loss 21882294
dgv3665n71 CNV Loss 21882294
nsv910783 CNV Loss 21882294
nsv910786 CNV Loss 21882294
dgv3666n71 CNV Loss 21882294
nsv910791 CNV Loss 21882294

Variation tolerance for DAPK3 Gene

Residual Variation Intolerance Score: 47.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.62; 31.19% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for DAPK3 Gene

HapMap Linkage Disequilibrium report
DAPK3
Human Gene Mutation Database (HGMD)
DAPK3

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for DAPK3 Gene

Disorders for DAPK3 Gene

MalaCards: The human disease database

(1) MalaCards diseases for DAPK3 Gene - From: GeneCards

Disorder Aliases PubMed IDs
recessive dystrophic epidermolysis bullosa
  • rdeb
- elite association - COSMIC cancer census association via MalaCards
Search DAPK3 in MalaCards View complete list of genes associated with diseases

Relevant External Links for DAPK3

Genetic Association Database (GAD)
DAPK3
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
DAPK3
genes like me logo Genes that share disorders with DAPK3: view

No data available for UniProtKB/Swiss-Prot and Genatlas for DAPK3 Gene

Publications for DAPK3 Gene

  1. ZIP kinase, a novel serine/threonine kinase which mediates apoptosis. (PMID: 9488481) Kawai T. … Akira S. (Mol. Cell. Biol. 1998) 2 3 4 23 67
  2. Link of Dlk/ZIP kinase to cell apoptosis and tumor suppression. (PMID: 20085750) Wu Y. … Yu L. (Biochem. Biophys. Res. Commun. 2010) 3 23
  3. Downregulation of ZIP kinase is associated with tumor invasion, metastasis and poor prognosis in gastric cancer. (PMID: 19117059) Bi J. … Guan X.Y. (Int. J. Cancer 2009) 3 23
  4. ZIP kinase plays a crucial role in androgen receptor-mediated transcription. (PMID: 18084323) Leister P. … Scheidtmann K.H. (Oncogene 2008) 3 23
  5. Novel ZIP kinase isoform lacks leucine zipper. (PMID: 17126281) Takamoto N. … Ikebe M. (Arch. Biochem. Biophys. 2006) 3 23

Products for DAPK3 Gene

Sources for DAPK3 Gene

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