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CENPF Gene(Protein Coding)

Centromere Protein F

GCID:
GC01P214603
GIFtS:
63
Genes Participants
UDN Logo

Aliases for CENPF Gene

Aliases for CENPF Gene

  • Centromere Protein F 2 3 5
  • Mitosin 2 3 4
  • Centromere Protein F, 350/400kDa (Mitosin) 2 3
  • Kinetochore Protein CENPF 3 4
  • AH Antigen 3 4
  • Cell-Cycle-Dependent 350K Nuclear Protein 3
  • Centromere Protein F, 350/400ka (Mitosin) 3
  • Centromere Protein F, 350/400kDa 2
  • CENP-F Kinetochore Protein 3
  • PRO1779 3
  • CILD31 3
  • STROMS 3
  • CENP-F 4
  • Hcp-1 3
  • CENF 3

External Ids for CENPF Gene

Previous GeneCards Identifiers for CENPF Gene

  • GC01P213428
  • GC01P210596
  • GC01P211392
  • GC01P211833
  • GC01P211165
  • GC01P212843
  • GC01P214776
  • GC01P185450

Summaries for CENPF Gene

Entrez Gene Summary for CENPF Gene

  • This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq, Jul 2008]

GeneCards Summary for CENPF Gene

CENPF (Centromere Protein F) is a Protein Coding gene. Diseases associated with CENPF include Ciliary Dyskinesia, Primary, 31 and Lethal Fetal Brain Malformation-Duodenal Atresia-Bilateral Renal Hypoplasia Syndrome. Among its related pathways are FOXM1 transcription factor network and Mitotic Metaphase and Anaphase. GO annotations related to this gene include protein homodimerization activity and transcription factor binding.

UniProtKB/Swiss-Prot for CENPF Gene

  • Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia.

Gene Wiki entry for CENPF Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CENPF Gene

Genomics for CENPF Gene

Products:

  1. Regulatory Element

Regulatory Elements for CENPF Gene

Enhancers for CENPF Gene
GeneHancer Identifier Score Enhancer Sources TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Other Gene Targets for Enhancer

Enhancers around CENPF on UCSC Golden Path with GeneCards custom track

Promoters for CENPF Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around CENPF on UCSC Golden Path with GeneCards custom track

Transcription factor binding sites by QIAGEN in the CENPF gene promoter:

Genomic Location for CENPF Gene

Chromosome:
1
Start:
214,603,179 bp from pter
End:
214,664,588 bp from pter
Size:
61,410 bases
Orientation:
Plus strand

Genomic View for CENPF Gene

Genes around CENPF on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
CENPF Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for CENPF Gene
GeneLoc Logo Genomic Neighborhood • Exon Structure • Gene Density

RefSeq DNA sequence for CENPF Gene

Proteins for CENPF Gene

Products:

  1. Antibody
  2. Protein
  3. Assay

  • Protein details for CENPF Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P49454-CENPF_HUMAN
    Recommended name:
    Centromere protein F
    Protein Accession:
    P49454
    Secondary Accessions:
    • Q13171
    • Q13246
    • Q5VVM7

    Protein attributes for CENPF Gene

    Size:
    3210 amino acids
    Molecular mass:
    367764 Da
    Quaternary structure:
    • Interacts with and STX4 (via C-terminus) (By similarity). Interacts (via N-terminus) with RBL1, RBL2 and SNAP25 (By similarity). Self-associates. Interacts with CENP-E and BUBR1 (via C-terminus). Interacts (via C-terminus) with NDE1, NDEL1 and RB1.

neXtProt entry for CENPF Gene

Proteomics data for CENPF Gene at MOPED

Protein Expression for CENPF Gene

See protein expression from ProteomicsDB, MOPED, PaxDb, and MaxQB

Post-translational modifications for CENPF Gene

  • Hyperphosphorylated during mitosis.
  • Ubiquitination at Lys 19, Lys 223, Lys 293, Lys 314, Lys 356, Lys 457, Lys 513, Lys 527, Lys 555, Lys 697, Lys 770, Lys 823, Lys 956, Lys 1022, Lys 1088, Lys 1341, Lys 1410, Lys 1906, Lys 2132, and Lys 2940
  • Modification sites at PhosphoSitePlus

Other Protein References for CENPF Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for CENPF (CENPF)
  • Abcam antibodies for CENPF

No data available for DME Specific Peptides for CENPF Gene

Domains & Families for CENPF Gene

Protein Domains for CENPF Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for CENPF Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

P49454

UniProtKB/Swiss-Prot:

CENPF_HUMAN :
  • Belongs to the centromere protein F family.
Family:
  • Belongs to the centromere protein F family.
genes like me logo Genes that share domains with CENPF: view

No data available for Gene Families for CENPF Gene

Function for CENPF Gene

Products:

  1. Animal Model
  2. CRISPR
  3. miRNA
  4. Inhibitory RNA
  5. Clone
  6. Cell Line
  7. Flow Cytometry

Molecular function for CENPF Gene

GENATLAS Biochemistry:
centromere protein F,component of the outer kinetochore plate
UniProtKB/Swiss-Prot Function:
Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia.

Gene Ontology (GO) - Molecular Function for CENPF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0008134 transcription factor binding IEA,IPI 15677469
GO:0042803 protein homodimerization activity IEA,IPI 7642639
genes like me logo Genes that share ontologies with CENPF: view

Phenotypes for CENPF Gene

MGI mutant phenotypes for CENPF:
inferred from 1 alleles
GenomeRNAi human phenotypes for CENPF:
genes like me logo Genes that share phenotypes with CENPF: view

Human Phenotype Ontology for CENPF Gene

HPO Id HPO Name Alternative Ids Definition Synonyms

Animal Model Products

miRNA for CENPF Gene

miRTarBase miRNAs that target CENPF

Inhibitory RNA Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Animal Models , Transcription Factor Targets and HOMER Transcription for CENPF Gene

Localization for CENPF Gene

Subcellular locations from UniProtKB/Swiss-Prot for CENPF Gene

Cytoplasm, perinuclear region. Nucleus matrix. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Relocalizes to the kinetochore/centromere (coronal surface of the outer plate) and the spindle during mitosis. Observed in nucleus during interphase but not in the nucleolus. At metaphase becomes localized to areas including kinetochore and mitotic apparatus as well as cytoplasm. By telophase, is concentrated within the intracellular bridge at either side of the mid-body.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for CENPF Gene COMPARTMENTS Subcellular localization image for CENPF gene
Compartment Confidence
cytoskeleton 5
cytosol 5
nucleus 5

Gene Ontology (GO) - Cellular Components for CENPF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000776 kinetochore IDA 7542657
GO:0005634 nucleus IEA,IDA 7542657
GO:0005635 nuclear envelope IDA 12154071
GO:0005654 nucleoplasm IDA --
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with CENPF: view

Pathways & Interactions for CENPF Gene

genes like me logo Genes that share pathways with CENPF: view

Pathways by source for CENPF Gene

1 Cell Signaling Technology pathway for CENPF Gene
2 BioSystems pathways for CENPF Gene

Gene Ontology (GO) - Biological Process for CENPF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000278 mitotic cell cycle IMP 7542657
GO:0001822 kidney development IMP 25564561
GO:0007062 sister chromatid cohesion TAS --
GO:0007094 mitotic spindle assembly checkpoint NAS 12974617
GO:0007264 small GTPase mediated signal transduction TAS --
genes like me logo Genes that share ontologies with CENPF: view

No data available for SIGNOR curated interactions for CENPF Gene

Drugs & Compounds for CENPF Gene

Products:

  1. Drug

(4) Drugs for CENPF Gene - From: Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
genes like me logo Genes that share compounds with CENPF: view

Transcripts for CENPF Gene

Products:

  1. CRISPR
  2. miRNA
  3. Inhibitory RNA
  4. Clone
  5. Flow Cytometry

mRNA/cDNA for CENPF Gene

(3) REFSEQ mRNAs :
(4) Additional mRNA sequences :
(256) Selected AceView cDNA sequences:
(6) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for CENPF Gene

Centromere protein F, 350/400kDa:
Representative Sequences:

Inhibitory RNA Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for CENPF Gene

No ASD Table

Relevant External Links for CENPF Gene

GeneLoc Exon Structure for
CENPF
ECgene alternative splicing isoforms for
CENPF

Expression for CENPF Gene

Products:

  1. Primer

mRNA expression in normal human tissues for CENPF Gene

mRNA expression in normal human tissues for CENPF Gene
mRNA expression by BioGPS for CENPF GenemRNA expression by GTEx for CENPF Gene

mRNA differential expression in normal tissues according to GTEx for CENPF Gene

This gene is overexpressed in Testis (x4.8) and Esophagus - Mucosa (x4.2).

Protein differential expression in normal tissues from HIPED for CENPF Gene

This gene is overexpressed in Nasal epithelium (43.0) and Bone marrow stromal cell (8.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for CENPF Gene



SOURCE GeneReport for Unigene cluster for CENPF Gene Hs.497741

genes like me logo Genes that share expression patterns with CENPF: view

Primer Products

No data available for mRNA Expression by UniProt/SwissProt and Protein tissue co-expression partners for CENPF Gene

Orthologs for CENPF Gene

This gene was present in the common ancestor of animals.

Orthologs for CENPF Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
 Mammalia CENPF 35
  • 84.66 (n)
  • 78.63 (a)
CENPF 36
  • 79 (a)
 OneToOne
dog
(Canis familiaris)
 Mammalia CENPF 35
  • 83.79 (n)
  • 76.66 (a)
-- 36
  • 84 (a)
 OneToMany
-- 36
  • 71 (a)
 OneToMany
mouse
(Mus musculus)
 Mammalia Cenpf 35
  • 75.67 (n)
  • 65.48 (a)
Cenpf 16
Cenpf 36
  • 66 (a)
 OneToOne
chimpanzee
(Pan troglodytes)
 Mammalia CENPF 35
  • 99.28 (n)
  • 98.78 (a)
CENPF 36
  • 99 (a)
 OneToOne
rat
(Rattus norvegicus)
 Mammalia Cenpf 35
  • 77.45 (n)
  • 67.93 (a)
oppossum
(Monodelphis domestica)
 Mammalia CENPF 36
  • 52 (a)
 OneToOne
platypus
(Ornithorhynchus anatinus)
 Mammalia CENPF 36
  • 54 (a)
 OneToOne
chicken
(Gallus gallus)
 Aves CENPF 35
  • 59.13 (n)
  • 44.33 (a)
CENPF 36
  • 41 (a)
 OneToOne
lizard
(Anolis carolinensis)
 Reptilia CENPF 36
  • 40 (a)
 OneToOne
tropical clawed frog
(Silurana tropicalis)
 Amphibia cenpf 35
  • 53.33 (n)
  • 42.05 (a)
zebrafish
(Danio rerio)
 Actinopterygii cenpf 36
  • 29 (a)
 OneToOne
worm
(Caenorhabditis elegans)
 Secernentea C02F12.7 37
  • 21 (a)
Species with no ortholog for CENPF:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for CENPF Gene

ENSEMBL:
Gene Tree for CENPF (if available)
TreeFam:
Gene Tree for CENPF (if available)

Paralogs for CENPF Gene

No data available for Paralogs for CENPF Gene

Variants for CENPF Gene

Sequence variations from dbSNP and Humsavar for CENPF Gene

SNP ID Clin Chr 01 pos Sequence Context AA Info Type
rs7289 - 214,663,767(-) CTGCC(A/C/G)TTGGA reference, missense
rs17023281 - 214,622,111(+) AACTA(C/T)GCCTG reference, missense
rs2070065 - 214,637,901(+) AGTCA(C/G)TCTGA reference, missense
rs3795524 - 214,640,439(-) CTCCA(C/G/T)ATAAG reference, missense
rs3795523 - 214,640,598(+) GTTAC(C/G)AAGAC reference, missense

Structural Variations from Database of Genomic Variants (DGV) for CENPF Gene

Variant ID Type Subtype PubMed ID
esv29119 CNV Loss 19812545
esv27146 CNV Gain+Loss 19812545
esv2145066 CNV Deletion 18987734
esv2722628 CNV Deletion 23290073
nsv160230 CNV Loss 16902084
esv1007536 CNV Insertion 20482838
nsv873165 CNV Loss 21882294
nsv436780 CNV Insertion 17901297

Variation tolerance for CENPF Gene

Residual Variation Intolerance Score: 98.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 20.93; 99.19% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for CENPF Gene

Human Gene Mutation Database (HGMD)
CENPF

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CENPF Gene

Disorders for CENPF Gene

MalaCards: The human disease database

(6) MalaCards diseases for CENPF Gene - From: OMIM, ClinVar, Orphanet, DISEASES, and GeneCards

- elite association - COSMIC cancer census association via MalaCards
Search CENPF in MalaCards View complete list of genes associated with diseases

UniProtKB/Swiss-Prot

CENPF_HUMAN
  • Ciliary dyskinesia, primary, 31 (CILD31) [MIM:616369]: A disorder characterized by abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia. Patients may exhibit randomization of left-right body asymmetry and situs inversus, due to dysfunction of monocilia at the embryonic node. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome. {ECO:0000269 PubMed:25564561}. Note=The disease is caused by mutations affecting the gene represented in this entry.

Relevant External Links for CENPF

Genetic Association Database (GAD)
CENPF
Human Genome Epidemiology (HuGE) Navigator
CENPF
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
CENPF
genes like me logo Genes that share disorders with CENPF: view

No data available for Genatlas for CENPF Gene

Publications for CENPF Gene

  1. CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis. (PMID: 7542657) Liao H. … Yen T.J. (J. Cell Biol. 1995) 3 4 23 67
  2. Chromosomal localization of the genes encoding the kinetochore proteins CENPE and CENPF to human chromosomes 4q24-->q25 and 1q32-->q41, respectively, by fluorescence in situ hybridization. (PMID: 7851898) Testa J.R. … Yen T.J. (Genomics 1994) 2 3 23
  3. Cdc20 is required for the post-anaphase, KEN-dependent degradation of centromere protein F. (PMID: 20053638) Gurden M.D. … Taylor S.S. (J. Cell. Sci. 2010) 3 23
  4. Involvement of CENP-F in histone methylation. (PMID: 20213041) Du J. … Zhu X. (Acta Biochim. Biophys. Sin. (Shanghai) 2010) 3 23
  5. Ki-67 expression is superior to mitotic count and novel proliferation markers PHH3, MCM4 and mitosin as a prognostic factor in thick cutaneous melanoma. (PMID: 20398247) Ladstein R.G. … Akslen L.A. (BMC Cancer 2010) 3 23

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Sources for CENPF Gene

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