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Aliases for CENPE Gene

Aliases for CENPE Gene

  • Centromere Protein E, 312kDa 2 3
  • Kinesin-Related Protein CENPE 3 4
  • CENP-E 3 4
  • Protein Phosphatase 1, Regulatory Subunit 61 3
  • Centromere Autoantigen E (312kD) 3
  • Centromere-Associated Protein E 3
  • Centromere Protein E (312kD) 2
  • Kinesin Family Member 10 3
  • Protein Phosphatase 1 2
  • Regulatory Subunit 61 2
  • Centromere Protein E 4
  • PPP1R61 3
  • MCPH13 3
  • KIF10 3

External Ids for CENPE Gene

Previous GeneCards Identifiers for CENPE Gene

  • GC04M104426
  • GC04M104486
  • GC04M104384
  • GC04M104246
  • GC04M104027
  • GC04M099760

Summaries for CENPE Gene

Entrez Gene Summary for CENPE Gene

  • Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Nov 2014]

GeneCards Summary for CENPE Gene

CENPE (Centromere Protein E, 312kDa) is a Protein Coding gene. Diseases associated with CENPE include microcephaly 13, primary, autosomal recessive and seckel syndrome. Among its related pathways are Class I MHC mediated antigen processing and presentation and Cell Cycle, Mitotic. GO annotations related to this gene include microtubule binding and kinetochore binding. An important paralog of this gene is KIF5C.

UniProtKB/Swiss-Prot for CENPE Gene

  • Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end-directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. The highly processive microtubule-dependent motor activity of CENPE serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss. Required for the efficient recruitment of BUBR1, MAD1 and MAD2 to attached and newly unattached kinetochores. Stimulates mammalian BUBR1 kinase activity. Accumulates just before mitosis at the G2 phase of the cell cycle.

Gene Wiki entry for CENPE Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for CENPE Gene

Genomics for CENPE Gene

Regulatory Elements for CENPE Gene

Genomic Location for CENPE Gene

Start:
103,105,806 bp from pter
End:
103,198,409 bp from pter
Size:
92,604 bases
Orientation:
Minus strand

Genomic View for CENPE Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for CENPE Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for CENPE Gene

Proteins for CENPE Gene

  • Protein details for CENPE Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q02224-CENPE_HUMAN
    Recommended name:
    Centromere-associated protein E
    Protein Accession:
    Q02224
    Secondary Accessions:
    • A6NKY9
    • A8K2U7
    • Q4LE75

    Protein attributes for CENPE Gene

    Size:
    2701 amino acids
    Molecular mass:
    316415 Da
    Quaternary structure:
    • Monomer. Interacts with CENPF, SEPT7, KIF18A, BUB1B kinase and PRC1. Interacts with NUF2; this interaction determines kinetochore localization. Interacts with SKAP; this interaction greatly favors SKAP binding to microtubules.
    SequenceCaution:
    • Sequence=BAE06078.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for CENPE Gene

    Alternative splice isoforms for CENPE Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for CENPE Gene

Proteomics data for CENPE Gene at MOPED

Post-translational modifications for CENPE Gene

  • Sumoylated with SUMO2 and SUMO3. The sumoylation mediates the association to the kinetochore.
  • The C-terminal inhibitory domain is phosphorylated. Phosphorylation relieves autoinhibition of the kinetochore motor (By similarity).
  • Ubiquitination at Lys1937
  • Modification sites at PhosphoSitePlus

Other Protein References for CENPE Gene

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for CENPE (CENPE)

No data available for DME Specific Peptides for CENPE Gene

Domains for CENPE Gene

Gene Families for CENPE Gene

HGNC:
  • KIF :Kinesins
  • PPP1R :Serine/threonine phosphatases / Protein phosphatase 1, regulatory subunits

Protein Domains for CENPE Gene

Suggested Antigen Peptide Sequences for CENPE Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q02224

UniProtKB/Swiss-Prot:

CENPE_HUMAN :
  • Q02224
Domain:
  • The protein is composed of a N-terminal kinesin-motor domain involved in the chromosome movements, a long coil-coiled region involved in the homodimerization and an inhibitory C-tail involved in autoinhibition of the N-terminal catalytic part.
  • Contains 1 kinesin motor domain.
Family:
  • Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.
genes like me logo Genes that share domains with CENPE: view

Function for CENPE Gene

Molecular function for CENPE Gene

GENATLAS Biochemistry: centromere protein E (312kDa),component of the outer kinetochore,microtubule associated kinesin motor protein,ATP dependent superfamily,required for the metaphase-anaphase transition in mitosis and meiosis II
UniProtKB/Swiss-Prot Function: Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end-directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. The highly processive microtubule-dependent motor activity of CENPE serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss. Required for the efficient recruitment of BUBR1, MAD1 and MAD2 to attached and newly unattached kinetochores. Stimulates mammalian BUBR1 kinase activity. Accumulates just before mitosis at the G2 phase of the cell cycle.

Gene Ontology (GO) - Molecular Function for CENPE Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003700 sequence-specific DNA binding transcription factor activity --
GO:0003777 microtubule motor activity IMP 7889940
GO:0004871 signal transducer activity --
GO:0005515 protein binding IPI 17535814
GO:0005524 ATP binding IEA --
genes like me logo Genes that share ontologies with CENPE: view
genes like me logo Genes that share phenotypes with CENPE: view

Animal Models for CENPE Gene

MGI Knock Outs for CENPE:

Animal Model Products

CRISPR Products

miRNA for CENPE Gene

miRTarBase miRNAs that target CENPE

No data available for Enzyme Numbers (IUBMB) , Transcription Factor Targeting and HOMER Transcription for CENPE Gene

Localization for CENPE Gene

Subcellular locations from UniProtKB/Swiss-Prot for CENPE Gene

Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Associates with kinetochores during congression (as early as prometaphase), relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for CENPE Gene COMPARTMENTS Subcellular localization image for CENPE gene
Compartment Confidence
cytoskeleton 5
cytosol 4
nucleus 4

Gene Ontology (GO) - Cellular Components for CENPE Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000775 chromosome, centromeric region IDA 11084331
GO:0000776 kinetochore IDA 11682612
GO:0000778 condensed nuclear chromosome kinetochore IEA --
GO:0000779 condensed chromosome, centromeric region IDA 19465021
GO:0000940 colocalizes_with condensed chromosome outer kinetochore TAS 9763420
genes like me logo Genes that share ontologies with CENPE: view

Pathways for CENPE Gene

genes like me logo Genes that share pathways with CENPE: view

Pathways by source for CENPE Gene

1 BioSystems pathway for CENPE Gene

Gene Ontology (GO) - Biological Process for CENPE Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000278 mitotic cell cycle TAS --
GO:0006200 obsolete ATP catabolic process --
GO:0006355 regulation of transcription, DNA-templated --
GO:0006614 SRP-dependent cotranslational protein targeting to membrane --
GO:0007018 microtubule-based movement TAS --
genes like me logo Genes that share ontologies with CENPE: view

Compounds for CENPE Gene

(7) Novoseek inferred chemical compound relationships for CENPE Gene

Compound -log(P) Hits PubMed IDs
monastrol 71.1 1
lonafarnib 66.4 2
nocodazole 40.8 2
vinblastine 17.4 2
atp 0 1
genes like me logo Genes that share compounds with CENPE: view

Transcripts for CENPE Gene

Unigene Clusters for CENPE Gene

Centromere protein E, 312kDa:
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for CENPE

Primer Products

  • OriGene qSTAR qPCR primer pairs in human,mouse,rat for CENPE
  • QuantiTect SYBR Green Assays in human,mouse,rat
  • Pre-validated RT² qPCR Primer Assay in human,mouse,rat
  • QuantiFast Probe-based Assays in human,mouse,rat

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for CENPE Gene

No ASD Table

Relevant External Links for CENPE Gene

GeneLoc Exon Structure for
CENPE
ECgene alternative splicing isoforms for
CENPE

Expression for CENPE Gene

mRNA expression in normal human tissues for CENPE Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for CENPE Gene

This gene is overexpressed in Testis (4.1).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for CENPE Gene

SOURCE GeneReport for Unigene cluster for CENPE Gene Hs.75573

genes like me logo Genes that share expressions with CENPE: view

In Situ Assay Products

No data available for mRNA Expression by UniProt/SwissProt for CENPE Gene

Orthologs for CENPE Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for CENPE Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia CENPE 35
  • 99.17 (n)
  • 98.78 (a)
CENPE 36
  • 99 (a)
OneToOne
cow
(Bos Taurus)
Mammalia CENPE 35
  • 86.28 (n)
  • 79.13 (a)
CENPE 36
  • 78 (a)
OneToOne
dog
(Canis familiaris)
Mammalia CENPE 35
  • 86.96 (n)
  • 80.38 (a)
CENPE 36
  • 80 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Cenpe 35
  • 73.88 (n)
  • 63.98 (a)
Cenpe 16
Cenpe 36
  • 65 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia CENPE 36
  • 50 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Cenpe 35
  • 74.9 (n)
  • 65.82 (a)
chicken
(Gallus gallus)
Aves CENPE 36
  • 44 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia cenpe-A 35
tropical clawed frog
(Silurana tropicalis)
Amphibia Str.17159 35
zebrafish
(Danio rerio)
Actinopterygii cenpe 36
  • 28 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta cana 36
  • 23 (a)
OneToMany
cmet 36
  • 23 (a)
OneToMany
cmet 37
  • 24 (a)
cana 37
  • 23 (a)
worm
(Caenorhabditis elegans)
Secernentea hcp-2 37
  • 19 (a)
corn
(Zea mays)
Liliopsida Zm.7330 35
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 29 (a)
OneToOne
Species with no ortholog for CENPE:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for CENPE Gene

ENSEMBL:
Gene Tree for CENPE (if available)
TreeFam:
Gene Tree for CENPE (if available)

Paralogs for CENPE Gene

Selected SIMAP similar genes for CENPE Gene using alignment to 3 proteins:

Pseudogenes.org Pseudogenes for CENPE Gene

genes like me logo Genes that share paralogs with CENPE: view

Variants for CENPE Gene

Sequence variations from dbSNP and Humsavar for CENPE Gene

SNP ID Clin Chr 04 pos Sequence Context AA Info Type MAF
rs1031803 -- 103,142,315(+) AAATG(A/C)TAATT intron-variant
rs1031804 -- 103,144,892(+) GTTTA(C/T)TGGTA intron-variant
rs1049660 -- 103,133,846(-) TGAAT(C/G)CATCA reference, missense
rs1381657 - 103,140,836(+) GCAGG(C/G)TTTCC missense, reference
rs1381658 -- 103,141,066(+) TTTAA(C/T)GTAAT synonymous-codon, reference

Structural Variations from Database of Genomic Variants (DGV) for CENPE Gene

Variant ID Type Subtype PubMed ID
dgv1656e1 CNV Complex 17122850
nsv830021 CNV Loss 17160897
esv2554056 CNV Loss 19546169
esv1606562 CNV Deletion 17803354
esv2728082 CNV Deletion 23290073
esv2675893 CNV Deletion 23128226
nsv830022 CNV Loss 17160897

Relevant External Links for CENPE Gene

HapMap Linkage Disequilibrium report
CENPE
Human Gene Mutation Database (HGMD)
CENPE

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for CENPE Gene

Disorders for CENPE Gene

MalaCards: The human disease database

MalaCards: The human disease database. (2) Diseases for CENPE Gene including...

Search for CENPE Gene in MalaCards »

UniProtKB/Swiss-Prot

CENPE_HUMAN
  • Microcephaly 13, primary, autosomal recessive (MCPH13) [MIM:616051]: A form of microcephaly, a disease defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. {ECO:0000269 PubMed:24748105}. Note=The disease is caused by mutations affecting the gene represented in this entry.

(2) Novoseek inferred disease relationships for CENPE Gene

Disease -log(P) Hits PubMed IDs
tumors 0 2
cancer 0 2

Relevant External Links for CENPE

Genetic Association Database (GAD)
CENPE
Human Genome Epidemiology (HuGE) Navigator
CENPE
genes like me logo Genes that share disorders with CENPE: view

Publications for CENPE Gene

  1. Mitotic HeLa cells contain a CENP-E-associated minus end-directed microtubule motor. (PMID: 7889940) Thrower D.A. … Wilson L. (EMBO J. 1995) 3 4 23
  2. CENP-E is a putative kinetochore motor that accumulates just before mitosis. (PMID: 1406971) Yen T.J. … Cleveland D.W. (Nature 1992) 3 4 23
  3. Farnesyl transferase inhibitors block the farnesylation of CENP-E and CENP-F and alter the association of CENP-E with the microtubules. (PMID: 10852915) Ashar H.R. … Kirschmeier P. (J. Biol. Chem. 2000) 3 4 23
  4. Crystal structure of the motor domain of the human kinetochore protein CENP-E. (PMID: 15236970) Garcia-Saez I. … Kozielski F. (J. Mol. Biol. 2004) 3 4 23
  5. Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation. (PMID: 15297875) Kurasawa Y. … Todokoro K. (EMBO J. 2004) 3 4 23

Products for CENPE Gene

Sources for CENPE Gene

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