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Aliases for AKAP8 Gene

Aliases for AKAP8 Gene

  • A-Kinase Anchoring Protein 8 2 3 5
  • A Kinase (PRKA) Anchor Protein 8 2 3
  • AKAP 95 3 4
  • AKAP-8 3 4
  • AKAP95 3 4
  • A-Kinase Anchor Protein, 95kDa 3
  • A-Kinase Anchor Protein 95 KDa 4
  • A-Kinase Anchor Protein 2
  • AKAP-95 3
  • 95kDa 2

External Ids for AKAP8 Gene

Previous GeneCards Identifiers for AKAP8 Gene

  • GC19M015843
  • GC19M015309
  • GC19M015325
  • GC19M015464
  • GC19M015032

Summaries for AKAP8 Gene

Entrez Gene Summary for AKAP8 Gene

  • This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011]

GeneCards Summary for AKAP8 Gene

AKAP8 (A-Kinase Anchoring Protein 8) is a Protein Coding gene. Among its related pathways are Activation of cAMP-Dependent PKA and Cell cycle_Chromosome condensation in prometaphase. GO annotations related to this gene include poly(A) RNA binding and protein kinase A regulatory subunit binding. An important paralog of this gene is AKAP8L.

UniProtKB/Swiss-Prot for AKAP8 Gene

  • Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II) (PubMed:9473338). Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring (PubMed:10601332, PubMed:10791967, PubMed:11964380). May help to deliver cyclin D/E to CDK4 to facilitate cell cycle progression (PubMed:14641107). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at Ser-10 of histone H3; in this function may act redundantly with AKAP8L (PubMed:16980585). Involved in nuclear retention of RPS6KA1 upon ERK activation thus inducing cell proliferation (PubMed:22130794). May be involved in regulation of DNA replication by acting as scaffold for MCM2 (PubMed:12740381). Enhances HMT activity of the KMT2 family MLL4/WBP7 complex and is involved in transcriptional regulation. In a teratocarcinoma cell line is involved in retinoic acid-mediated induction of developmental genes implicating H3 Lys-4 methylation (PubMed:23995757). May be involved in recruitment of active CASP3 to the nucleus in apoptotic cells (PubMed:16227597). May act as a carrier protein of GJA1 for its transport to the nucleus (PubMed:26880274). Seems to involved in modulation of rDNA transcription. Preferentially binds GC-rich DNA in vitro and associates to GC-rich ribosomal RNA promoters (PubMed:26683827). Involved in modulation of Toll-like receptor signaling. Required for the cAMP-dependent suppression of TNF-alpha in early stages of LPS-induced macrophage activation; the function probably implicates targeting of PKA to NFKB1 (By similarity).

Gene Wiki entry for AKAP8 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for AKAP8 Gene

Genomics for AKAP8 Gene

Regulatory Elements for AKAP8 Gene

Enhancers for AKAP8 Gene
GeneHancer Identifier Score Enhancer Sources TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Other Gene Targets for Enhancer

Enhancers around AKAP8 on UCSC Golden Path with GeneCards custom track

Promoters for AKAP8 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around AKAP8 on UCSC Golden Path with GeneCards custom track

Genomic Location for AKAP8 Gene

Chromosome:
19
Start:
15,353,385 bp from pter
End:
15,379,828 bp from pter
Size:
26,444 bases
Orientation:
Minus strand

Genomic View for AKAP8 Gene

Genes around AKAP8 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
AKAP8 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for AKAP8 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for AKAP8 Gene

Proteins for AKAP8 Gene

  • Protein details for AKAP8 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O43823-AKAP8_HUMAN
    Recommended name:
    A-kinase anchor protein 8
    Protein Accession:
    O43823

    Protein attributes for AKAP8 Gene

    Size:
    692 amino acids
    Molecular mass:
    76108 Da
    Quaternary structure:
    • Binds to the PKA RII-alpha regulatory subunit PRKAR2A (phosphorylated at Thr-54) during mitosis (PubMed:9473338, PubMed:10601332, PubMed:10764601, PubMed:11591814). Interacts (via C-terminus) with FIGN (By similarity). Interacts with NCAPD2, CCND1, MCM2, RPS6KA1, PDE4A (PubMed:10601332, PubMed:11964380, PubMed:11591814, PubMed:12740381, PubMed:14641107, PubMed:15470020, PubMed:22130794). Interacts with CCND3, CCNE1, DDX5, CASP3. Interacts with NFKB1; detetcted in the cytoplasm. Interacts with MYCBP; MYCBP is translocated to the nucleus and the interaction prevents the association of the PKA catalytic subunit leading to suppression of PKA activity (By similarity). Interacts with DPY30; mediating AKAP8 association with at least the MLL4/WBP7 HMT complex (PubMed:23995757). Interacts with HDAC3; increased during mitosis (PubMed:16980585). Interacts with GJA1; in the nucleus and in the nuclear membrane; the nuclear association increases with progress of cell cycle G1, S and G2 phase and decreases in M phase (PubMed:26880274).

neXtProt entry for AKAP8 Gene

Proteomics data for AKAP8 Gene at MOPED

Post-translational modifications for AKAP8 Gene

  • Phosphorylated on tyrosine residues probably by SRC subfamily protein kinases; multiple phosphorylation is leading to dissociation from nuclear structures implicated in chromatin structural changes.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for AKAP8 Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for AKAP8 Gene

Domains & Families for AKAP8 Gene

Gene Families for AKAP8 Gene

Protein Domains for AKAP8 Gene

Suggested Antigen Peptide Sequences for AKAP8 Gene

Graphical View of Domain Structure for InterPro Entry

O43823

UniProtKB/Swiss-Prot:

AKAP8_HUMAN :
  • Belongs to the AKAP95 family.
  • Contains 2 C2H2 AKAP95-type zinc fingers.
Family:
  • Belongs to the AKAP95 family.
Similarity:
  • Contains 2 C2H2 AKAP95-type zinc fingers.
genes like me logo Genes that share domains with AKAP8: view

Function for AKAP8 Gene

Molecular function for AKAP8 Gene

GENATLAS Biochemistry:
A kinase (PRKA regulatory subunit RIIA) anchor protein 8,located in the nucleus in quiescent fibroblasts,excluding RIIA,suggesting that the interaction is cell cycle dependent,homolog to rat AKAP95
UniProtKB/Swiss-Prot Function:
Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II) (PubMed:9473338). Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring (PubMed:10601332, PubMed:10791967, PubMed:11964380). May help to deliver cyclin D/E to CDK4 to facilitate cell cycle progression (PubMed:14641107). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at Ser-10 of histone H3; in this function may act redundantly with AKAP8L (PubMed:16980585). Involved in nuclear retention of RPS6KA1 upon ERK activation thus inducing cell proliferation (PubMed:22130794). May be involved in regulation of DNA replication by acting as scaffold for MCM2 (PubMed:12740381). Enhances HMT activity of the KMT2 family MLL4/WBP7 complex and is involved in transcriptional regulation. In a teratocarcinoma cell line is involved in retinoic acid-mediated induction of developmental genes implicating H3 Lys-4 methylation (PubMed:23995757). May be involved in recruitment of active CASP3 to the nucleus in apoptotic cells (PubMed:16227597). May act as a carrier protein of GJA1 for its transport to the nucleus (PubMed:26880274). Seems to involved in modulation of rDNA transcription. Preferentially binds GC-rich DNA in vitro and associates to GC-rich ribosomal RNA promoters (PubMed:26683827). Involved in modulation of Toll-like receptor signaling. Required for the cAMP-dependent suppression of TNF-alpha in early stages of LPS-induced macrophage activation; the function probably implicates targeting of PKA to NFKB1 (By similarity).

Gene Ontology (GO) - Molecular Function for AKAP8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003677 DNA binding IEA --
GO:0003690 double-stranded DNA binding IEA --
GO:0042826 histone deacetylase binding IDA 16980585
GO:0044822 poly(A) RNA binding IDA 22658674
genes like me logo Genes that share ontologies with AKAP8: view
genes like me logo Genes that share phenotypes with AKAP8: view

Animal Model Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for AKAP8 Gene

Localization for AKAP8 Gene

Subcellular locations from UniProtKB/Swiss-Prot for AKAP8 Gene

Nucleus. Nucleus matrix. Nucleus, nucleolus. Cytoplasm. Note=Associated with the nuclear matrix in interphase and redistributes mostly to chromatin at mitosis. However, mitotic chromatin localization has been questioned. Upon nuclear reassembly at the end of mitosis, is sequestered into the daughter nuclei where it re-acquires an interphase distribution. Localized to the nucleolus in interphase. Colocalizes with GJA1 at the nuclear membrane specifically during cell cycle G1/S phase. {ECO:0000269 PubMed:10601332, ECO:0000269 PubMed:26683827, ECO:0000269 PubMed:26880274}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for AKAP8 Gene COMPARTMENTS Subcellular localization image for AKAP8 gene
Compartment Confidence
nucleus 5
cytosol 2
golgi apparatus 2
mitochondrion 2

Gene Ontology (GO) - Cellular Components for AKAP8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA,TAS 9473338
GO:0005730 nucleolus IEA --
GO:0016020 membrane IDA 19946888
genes like me logo Genes that share ontologies with AKAP8: view

Pathways & Interactions for AKAP8 Gene

genes like me logo Genes that share pathways with AKAP8: view

Pathways by source for AKAP8 Gene

Gene Ontology (GO) - Biological Process for AKAP8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006355 regulation of transcription, DNA-templated IEA --
GO:0007067 mitotic nuclear division TAS 9473338
GO:0007076 mitotic chromosome condensation IEA --
genes like me logo Genes that share ontologies with AKAP8: view

No data available for SIGNOR curated interactions for AKAP8 Gene

Drugs & Compounds for AKAP8 Gene

(4) Drugs for AKAP8 Gene - From: ApexBio, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
cyclic amp Experimental Pharma 0
PKA inhibitor fragment (6-22) amide Pharma 0

(1) ApexBio Compounds for AKAP8 Gene

Compound Action Cas Number
PKA inhibitor fragment (6-22) amide 121932-06-7
genes like me logo Genes that share compounds with AKAP8: view

Transcripts for AKAP8 Gene

Unigene Clusters for AKAP8 Gene

A kinase (PRKA) anchor protein 8:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for AKAP8 Gene

ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6a · 6b ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15
SP1: -
SP2: - - -
SP3: - - - - -
SP4:

Relevant External Links for AKAP8 Gene

GeneLoc Exon Structure for
AKAP8
ECgene alternative splicing isoforms for
AKAP8

Expression for AKAP8 Gene

mRNA expression in normal human tissues for AKAP8 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for AKAP8 Gene

This gene is overexpressed in Adrenal (19.4) and Peripheral blood mononuclear cells (10.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MOPED, and MaxQB for AKAP8 Gene



SOURCE GeneReport for Unigene cluster for AKAP8 Gene Hs.594496

mRNA Expression by UniProt/SwissProt for AKAP8 Gene

O43823-AKAP8_HUMAN
Tissue specificity: Highly expressed in heart, liver, skeletal muscle, kidney and pancreas. Expressed in mature dendritic cells.
genes like me logo Genes that share expression patterns with AKAP8: view

Protein tissue co-expression partners for AKAP8 Gene

- Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA differential expression in normal tissues for AKAP8 Gene

Orthologs for AKAP8 Gene

This gene was present in the common ancestor of chordates.

Orthologs for AKAP8 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia AKAP8 35
  • 85.46 (n)
  • 85.9 (a)
AKAP8 36
  • 84 (a)
OneToOne
dog
(Canis familiaris)
Mammalia AKAP8 35
  • 83.58 (n)
  • 82.99 (a)
AKAP8 36
  • 81 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Akap8 35
  • 81.54 (n)
  • 79.88 (a)
Akap8 16
Akap8 36
  • 77 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia AKAP8 35
  • 99.08 (n)
  • 98.62 (a)
AKAP8 36
  • 99 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Akap8 35
  • 81.65 (n)
  • 80.73 (a)
oppossum
(Monodelphis domestica)
Mammalia AKAP8 36
  • 61 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia -- 36
  • 83 (a)
OneToMany
-- 36
  • 53 (a)
OneToMany
chicken
(Gallus gallus)
Aves AKAP8 35
  • 67.15 (n)
  • 60.24 (a)
lizard
(Anolis carolinensis)
Reptilia AKAP8 36
  • 54 (a)
OneToOne
Species with no ortholog for AKAP8:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)
  • zebrafish (Danio rerio)

Evolution for AKAP8 Gene

ENSEMBL:
Gene Tree for AKAP8 (if available)
TreeFam:
Gene Tree for AKAP8 (if available)

Paralogs for AKAP8 Gene

Paralogs for AKAP8 Gene

(1) SIMAP similar genes for AKAP8 Gene using alignment to 4 proteins:

Pseudogenes.org Pseudogenes for AKAP8 Gene

genes like me logo Genes that share paralogs with AKAP8: view

Variants for AKAP8 Gene

Sequence variations from dbSNP and Humsavar for AKAP8 Gene

SNP ID Clin Chr 19 pos Sequence Context AA Info Type
VAR_036534 A breast cancer sample
rs12976674 -- 15,376,072(+) ctaca(A/G)gcacg intron-variant
rs12980485 -- 15,362,384(+) ctctc(C/T)ccacg intron-variant
rs12980728 -- 15,357,172(+) gccag(A/G)ctggt intron-variant
rs12981013 -- 15,363,284(+) gcctc(C/T)gccca intron-variant

Structural Variations from Database of Genomic Variants (DGV) for AKAP8 Gene

Variant ID Type Subtype PubMed ID
nsv833765 CNV Loss 17160897
nsv911133 CNV Loss 21882294

Variation tolerance for AKAP8 Gene

Residual Variation Intolerance Score: 82.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.19; 51.99% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for AKAP8 Gene

HapMap Linkage Disequilibrium report
AKAP8

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for AKAP8 Gene

Disorders for AKAP8 Gene

Relevant External Links for AKAP8

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
AKAP8

No disorders were found for AKAP8 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for AKAP8 Gene

Publications for AKAP8 Gene

  1. Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein, AKAP95. (PMID: 9473338) Eide T. … Tasken K. (Exp. Cell Res. 1998) 2 3 4 23 67
  2. A-kinase anchoring in dendritic cells is required for antigen presentation. (PMID: 19277197) Schillace R.V. … Carr D.W. (PLoS ONE 2009) 3 23
  3. A-kinase-anchoring protein 95 functions as a potential carrier for the nuclear translocation of active caspase 3 through an enzyme-substrate-like association. (PMID: 16227597) Kamada S. … Hunter T. (Mol. Cell. Biol. 2005) 3 23
  4. A novel partner for D-type cyclins: protein kinase A-anchoring protein AKAP95. (PMID: 14641107) Arsenijevic T. … Pirson I. (Biochem. J. 2004) 3 23
  5. A-kinase anchoring proteins interact with phosphodiesterases in T lymphocyte cell lines. (PMID: 15470020) Asirvatham A.L. … Carr D.W. (J. Immunol. 2004) 3 23

Products for AKAP8 Gene

Sources for AKAP8 Gene

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