ZNF645 Gene
protein-coding GIFtS: 43
GCID: GC0XP022200
|
|
zinc finger protein 645
| |
Aliases
for ZNF645 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Zinc Finger Protein 6451 2 3 | | HAKAIL1 2 | | FLJ257351 | | E3 Ubiquitin Ligase2 | | E3 Ubiquitin-Protein Ligase ZNF6452 | | EC 6.3.2.-3 |
Export aliases for ZNF645 gene to outside databasesPrevious GC identifers: GC0XP022051 GC0XP020031 |
Summaries
for ZNF645 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for ZNF645:
This gene encodes a member of the zinc finger domain-containing protein family. This family member contains both aRING-type and a C2H2-type of zinc finger domain, and it may function as an E3 ubiquitin-protein ligase. Proteinlocalization suggests a role in human sperm production and quality control. (provided by RefSeq, Aug 2011)
UniProtKB/Swiss-Prot: ZN645_HUMAN, Q8N7E2Function: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Mayoperate on tyrosine-phosphorylated SRC substrates
|
Genomic Views
for ZNF645 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000023.10 NC_018934.1 NT_167197.1
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the ZNF645 gene promoter:
AML1a CUTL1 S8 POU2F1 FOXJ2 (long isoform) POU2F1a POU2F1b POU2F1c FOXJ2 MRF-2
Other transcription factors
Search SABiosciences Chromatin IP Primers for ZNF645
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ZNF645 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: Xp22.11 Ensembl cytogenetic band: Xp22.11 HGNC cytogenetic band: Xp22.11ZNF645 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome X GeneLoc Exon Structure GeneLoc location for GC0XP022200: view genomic region
(about GC identifiers)
Start:
|
22,291,060 bp from pter |
End:
|
22,292,574 bp from pter |
Size:
|
1,515 bases |
Orientation:
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plus strand |
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Proteins
for ZNF645 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: ZN645_HUMAN, Q8N7E2 (See
protein sequence)Recommended Name: E3 ubiquitin-protein ligase ZNF645 Size: 425 amino acids; 48785 Da
Subunit: Homodimer (By similarity)
Subcellular location: Cytoplasm. Note=Localized over the postacrosomal perinuclear theca region and the entire lengthof sperm tail
Secondary accessions: A0AV29 A0AV31 Q6DJY9Explore the universe of human proteins at neXtProt for ZNF645: NX_Q8N7E2
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q8N7E2
ZNF645 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_689790.1
ENSEMBL proteins: ENSP00000323348
Human Recombinant Protein Products:
Gene Ontology (GO): 1 cellular component term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005737 | cytoplasm |
IEA | -- |
ZNF645 for ontologies About GeneDecksing
ZNF645 Antibody Products:
Assay Products for ZNF645: |
Protein
Domains /
Families
for ZNF645 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
ZNF645 for domains About GeneDecksing
5 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q8N7E2ProtoNet protein and cluster: Q8N7E2 1 Blocks protein family: IPB001841 Zn-finger
UniProtKB/Swiss-Prot: ZN645_HUMAN, Q8N7E2Domain: The HYB domain forms a phosphotyrosine-binding pocket upon dimerization, and mediates as well the recognitionof its flanking acidic amino acids (By similarity)Similarity: Contains 1 C2H2-type zinc fingerSimilarity: Contains 1 RING-type zinc finger |
Function
for ZNF645 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: ZN645_HUMAN, Q8N7E2Function: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Mayoperate on tyrosine-phosphorylated SRC substratesEnzyme Number (IUBMB): EC 6.3.2.-1
Clone
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ZNF645 |
Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0008270 | zinc ion binding |
IEA | -- | | GO:0016874 | ligase activity |
IEA | -- |
ZNF645 for ontologies About GeneDecksing
1 GenomeRNAi human phenotype for ZNF645:
|
Pathways & Interactions
for ZNF645 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
UniProtKB/Swiss-Prot: ZN645_HUMAN, Q8N7E2Pathway: Protein modification; protein ubiquitinationInteractions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for ZNF645
STRING Interaction
Network Preview (showing 1 interactants - click image to see more details)
1 Interacting protein for ZNF645 (ENSP000003233484) via UniProtKB, MINT, STRING, and/or I2D About this table
Gene Ontology (GO): 1 biological process term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0016567 | protein ubiquitination |
IEA | -- |
ZNF645 for ontologies About GeneDecksing
|
Drugs & Compounds
for ZNF645 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for ZNF645
Search CenterWatch for drugs/clinical trials and news about ZNF645 / ZN645
|
Transcripts
for ZNF645 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for ZNF645 gene: NM_152577.3 Unigene Cluster for ZNF645: Zinc finger protein 645 Hs.132485 [show with all ESTs]Unigene Representative Sequence: GQ3553361 Ensembl transcript including schematic representation, and UCSC links where relevant: ENST00000323684(uc004dai.2)
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for ZNF645
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for ZNF645
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: ZNF645 (NM_152577) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for ZNF645 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ZNF645 |
Additional cDNA sequence: AK098601.1 BC074910.2 BC126190.1 BC126192.1 GQ355336.1 1 DOTS entry: DT.40129332 6 AceView cDNA sequences: BX103567 AA922253 BC074910 NM_152577 AK098601 BP370443
GeneLoc Exon Structure
|
Expression
for ZNF645 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| ZNF645 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: GTCGATCACA
About this image
See ZNF645 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for ZNF645
SOURCE GeneReport for Unigene cluster: Hs.132485
UniProtKB/Swiss-Prot: ZN645_HUMAN, Q8N7E2Tissue specificity: Exclusively expressed in testis and sperm, including spermatocytes, round and elongated spermatids,and Leydig cells
SABiosciences Expression via Pathway-Focused PCR Array including ZNF645: | Ubiquitin Ligases in human mouse rat |
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for ZNF645
Browse OriGene validated miRNA SYBR primer pairs
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Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ZNF645 |
Orthologs
for ZNF645 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of eukaryotes.
Orthologs for ZNF645 gene from 6/11 species (see all 11) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
chicken
(Gallus gallus) |
Aves |
HAKAI_CHICK6 |
E3 ubiquitin-protein ligase Hakai |
44(a) |
1 → many |
1(15799779-15806215) |
lizard
(Anolis carolinensis) |
Reptilia |
-- |
-- |
43(a) |
1 → many |
5(97639568-97645224) |
zebrafish
(Danio rerio) |
Actinopterygii |
CU041386.16cbll16 |
--Cas-Br-M (murine) ecotropic retroviral transformin... |
36(a)35(a) |
many ↔ manymany ↔ many |
25(20230700-20237657) 4(17904979-17909255) |
fruit fly
(Drosophila melanogaster) |
Insecta |
Hakai6 |
Hakai |
16(a) |
1 → many |
2L(19580612-19582474) |
thale cress
(Arabidopsis thaliana) |
eudicotyledons |
AT5G011606 |
e-cadherin binding protein-like protein |
15(a) |
1 → many |
5(54010-55856) |
rice
(Oryza sativa) |
Liliopsida |
-- |
ZOS10-06 - C2H2 zinc finger protein, expressed |
12(a) |
1 → many |
10(18723094-18727119) |
Species with no ortholog for ZNF645
ENSEMBL Gene Tree for ZNF645 (if available)
TreeFam Gene Tree for ZNF645 (if available) |
Paralogs
for ZNF645 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for ZNF645 gene
- CBLL12
1 SIMAP similar gene for ZNF645 using alignment to 2 protein entries: ZN645_HUMAN (see all proteins):CBLL1
ZNF645 for paralogs About GeneDecksing
|
Genomic Variants
for ZNF645 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr X pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for ZNF645 (22291060 - 22292574 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for ZNF645: --
Locus Specific Mutation Databases (LSDB): ZNF645
| SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing ZNF645 |
|
Disorders
/ Diseases
for ZNF645 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
| -- |
Publications
for ZNF645 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for ZNF645 gene integrated from 9 sources:
(articles sorted by number of sources associating them with ZNF645) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Human RING finger protein ZNF645 is a novel testis-sp ecific E3 ubiquitin ligase. (PubMed id 20657603)1, 2 Liu Y.Q....Zhang S.Z. (2010)
- The DNA sequence of the human X chromosome. (PubMed id 15772651)1, 2 Ross M.T.... Bentley D.R. (2005)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- Structure of a novel phosphotyrosine-binding domain in Hakai that targets E-cadherin. (PubMed id 22252131)2 Mukherjee M....Sivaraman J. (2012)
- Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (2011)
- Promoter demethylation mediates the expression of ZNF 645, a novel cancer/testis gene. (PubMed id 20587329)1 Bai G....Zhang S. (2010)
- Toward a confocal subcellular atlas of the human proteome. (PubMed id 18029348)1 Barbe L....Andersson-Svahn H. (2008)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
|
External Searches for ZNF645 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing ZNF645 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing ZNF645 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing ZNF645 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for ZNF645 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for ZNF645 gene:
Search GeneIP for patents involving ZNF645
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for ZNF645 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
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 | | | ZNF645 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ZNF645 |
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| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ZNF645 |
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