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ZNF543 Gene

protein-coding   GIFtS: 49
GCID: GC19P057831

Zinc Finger Protein 543

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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Zinc Finger Protein 5431 2

External Ids:    HGNC: 252811   Entrez Gene: 1259192   Ensembl: ENSG000001782297   UniProtKB: Q08ER83   

Export aliases for ZNF543 gene to outside databases

Previous GC identifers: GC00U914381 GC19P062525 GC19P062523 GC19P054146


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for ZNF543 Gene:
ZNF543 (zinc finger protein 543) is a protein-coding gene. An important paralog of this gene is ZNF80.

UniProtKB/Swiss-Prot: ZN543_HUMAN, Q08ER8
Function: May be involved in transcriptional regulation




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000019.9  NC_018930.2  NT_011109.17  
Regulatory elements:
   Regulatory transcription factor binding sites in the ZNF543 gene promoter:
         POU2F1   p300   POU2F1a   Ik-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidZNF543 promoter sequence
   Search Chromatin IP Primers for ZNF543

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat ZNF543


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 19q13.43   Ensembl cytogenetic band:  19q13.43   HGNC cytogenetic band: 19q13.43

ZNF543 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ZNF543 gene location

GeneLoc information about chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19P057831:  view genomic region     (about GC identifiers)

Start:
57,831,865 bp from pter      End:
57,842,144 bp from pter
Size:
10,280 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: ZN543_HUMAN, Q08ER8 (See protein sequence)
Recommended Name: Zinc finger protein 543  
Size: 600 amino acids; 68404 Da
Secondary accessions: Q495U9 Q495V0 Q6ZMP4 Q8NCX4

Explore the universe of human proteins at neXtProt for ZNF543: NX_Q08ER8

Explore proteomics data for ZNF543 at MOPED

Post-translational modifications: 

  • Ubiquitination2 at Lys337, Lys477
  • Modification sites at PhosphoSitePlus

  • See ZNF543 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_998763.2  
    ENSEMBL proteins: 
     ENSP00000322545  
    Reactome Protein details: Q08ER8

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    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    ZNF: Zinc fingers, C2H2-type

    4 InterPro protein domains:
     IPR001909 Krueppel-associated_box
     IPR015880 Znf_C2H2-like
     IPR013087 Znf_C2H2/integrase_DNA-bd
     IPR007087 Znf_C2H2

    Graphical View of Domain Structure for InterPro Entry Q08ER8

    ProtoNet protein and cluster: Q08ER8

    1 Blocks protein domain: IPB001909 KRAB box

    UniProtKB/Swiss-Prot: ZN543_HUMAN, Q08ER8
    Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family
    Similarity: Contains 13 C2H2-type zinc fingers
    Similarity: Contains 1 KRAB domain


    Find genes that share domains with ZNF543           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: ZN543_HUMAN, Q08ER8
    Function: May be involved in transcriptional regulation

         Gene Ontology (GO): 3 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003676nucleic acid binding ----
    GO:0003677DNA binding IEA--
    GO:0046872metal ion binding IEA--
         
    Find genes that share ontologies with ZNF543           About GenesLikeMe


    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for ZNF543
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    miRNA
    Products:
        
    miRTarBase miRNAs that target ZNF543:
    hsa-mir-30b-5p (MIRT023415)

    Block miRNA regulation of human, mouse, rat ZNF543 using miScript Target Protectors
    1 qRT-PCR Assays for microRNA that regulate ZNF543:
    hsa-miR-520g
    SwitchGear 3'UTR luciferase reporter plasmidZNF543 3' UTR sequence
    Inhib. RNA
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    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    ZN543_HUMAN, Q08ER8: Nucleus (Probable)
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus4
    cytosol2

    Gene Ontology (GO): 2 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005622intracellular ----
    GO:0005634nucleus IEA--

    Find genes that share ontologies with ZNF543           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for ZNF543 About    
    See pathways by source

    SuperPathContained pathways About
    1Gene Expression
    Generic Transcription Pathway0.40
    Gene Expression0.40


    Find genes that share SuperPaths with ZNF543           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 Reactome Pathway for ZNF543
        Generic Transcription Pathway


        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for ZNF543
    Interactions:

        Search GeneGlobe Interaction Network for ZNF543

    STRING Interaction Network Preview (showing 1 interactants - click image to see more details)

    1 Interacting protein for ZNF543 (ENSP000003225454) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ELAVL1ENSP000003852694STRING: ENSP00000385269
    About this table

    Gene Ontology (GO): 2 biological process terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006351transcription, DNA-templated IEA--
    GO:0006355regulation of transcription, DNA-templated IEA--

    Find genes that share ontologies with ZNF543           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for ZNF543 (ZN543)



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for ZNF543 gene: 
    NM_213598.3  

    Unigene Cluster for ZNF543:

    Zinc finger protein 543
    Hs.202544  [show with all ESTs]
    Unigene Representative Sequence: NM_213598
    1 Ensembl transcript including schematic representation, and UCSC links where relevant:
    ENST00000321545(uc002qoi.2)
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat ZNF543 using miScript Target Protectors
    1 qRT-PCR Assays for microRNA that regulate ZNF543:
    hsa-miR-520g
    SwitchGear 3'UTR luciferase reporter plasmidZNF543 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for ZNF543
    Predesigned siRNA for gene silencing in human, mouse, rat ZNF543
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    GenScript: all cDNA clones in your preferred vector: ZNF543 (NM_213598)
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      QuantiTect SYBR Green Assays in human, mouse, rat ZNF543
      QuantiFast Probe-based Assays in human, mouse, rat ZNF543

    Additional mRNA sequence: 

    AF024696.1 AK131547.1 AL834534.1 BC101012.2 BC101013.1 BC101014.2 BC101015.1 

    2 DOTS entries:

    DT.100775917  DT.95358759 

    24 AceView cDNA sequences:

    AI261710 BG249309 AW949165 NM_213598 AL834534 AK131547 BF909478 AI697585 
    BE673681 BX478666 BF433113 AA346847 BF741901 AA376773 CD688279 BE176914 
    BF747956 AF024708 AF024696 BF927642 AI885371 AI458924 AW629313 BF364317 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    ZNF543 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TATTCACTTT
    ZNF543 Expression
    About this image

    ZNF543 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    ZNF543 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.202544
        Custom PCR Arrays for ZNF543
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ZNF543

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of chordates.

    Orthologs for ZNF543 gene from 3 species (see representative species )    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chimpanzee
    (Pan troglodytes)
    Mammalia ZNF5431 zinc finger protein 543 98.94(n)
    98.5(a)
      469036  XM_003316741.2  XP_003316789.1 
    lizard
    (Anolis carolinensis)
    Reptilia --
    Uncharacterized protein
    42(a)
    1 → many
    GL344452.1(42241-44649)
    zebrafish
    (Danio rerio)
    Actinopterygii si:dkey-7j22.36
    CU138542.36
    Uncharacterized protein
    51(a)
    45(a)
    many ↔ many
    many ↔ many
    4(50336373-50347976) ENSDARG00000088640
    Zv9_scaffold3506(185121-205552) ENSDARG00000088627


    ENSEMBL Gene Tree for ZNF543 (if available)
    TreeFam Gene Tree for ZNF543 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for ZNF543 gene
    ZNF802  ZNF4602  ZNF5992  ZNF5502  ZNF8052  ZNF2642  
    Selected SIMAP similar genes for ZNF543 using alignment to 1 protein entry:     ZN543_HUMAN(see all similar genes):
    ZNF805    ZNF264    ZNF78L1    kr-znf3    ZNF818P    DKFZp666C237
    HZF30    zf30    DKFZp686M04222    HZF16    ZFS-1    ZNF501
    ZNF80    ZSCAN16    ZNF550    ZNF146    ZNF271    ZNF502

    Find genes that share paralogs with ZNF543           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for ZNF543 (see all 513)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 19 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1496412661,2
    --57829955(+) GTTTTA/CCTGTG 1 -- us2k10--------
    rs1433212871,2
    C--57830226(+) GCAAGG/TGTGCA 1 -- us2k10--------
    rs45772011,2
    H--57830305(+) TGAGTG/AAAGCA 1 -- us2k14Minor allele frequency- A:0.00NS EA 418
    rs1916743171,2
    --57830314(+) CACCAA/TGGTGT 1 -- us2k10--------
    rs1140825401,2
    F--57830424(+) ATAACA/GTGCAG 1 -- us2k11Minor allele frequency- G:0.02WA 118
    rs1845795291,2
    --57830468(+) GAGAAC/TGGCCC 1 -- us2k10--------
    rs1152648251,2
    C,F--57830489(+) GCCATA/GTTGCT 1 -- us2k13Minor allele frequency- G:0.05WA NA EA 358
    rs1996081251,2
    C--57830551(+) TATAT-/ACATACA 1 -- us2k10--------
    rs1115121511,2
    C--57830561(+) ACATAC/TATACA 1 -- us2k10--------
    rs747278001,2
    C--57830581(+) ATATAC/TATACA 1 -- us2k12Minor allele frequency- T:0.25NA 4

    HapMap Linkage Disequilibrium report for ZNF543 (57831865 - 57842144 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 7 variations for ZNF543:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2659236CNV Deletion23128226
    nsv821238CNV Deletion20802225
    esv2531361CNV Insertion19546169
    nsv819877CNV Loss19587683
    esv25537CNV Gain19812545
    nsv819727CNV Gain19587683
    dgv602n67CNV Gain20364138

    Human Gene Mutation Database (HGMD): ZNF543
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing ZNF543
    DNA2.0 Custom Variant and Variant Library Synthesis for ZNF543

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    Find genes that share disorders with ZNF543           About GenesLikeMe


    Export disorders for ZNF543 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for ZNF543 gene integrated from 10 sources:
    (articles sorted by number of sources associating them with ZNF543)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. The proteomic analysis of endogenous FAT10 substrates identifies p62/SQSTM1 as a substrate of FAT10ylation. (PubMed id 22797925)1 Aichem A....Groettrup M. (J. Cell. Sci. 2012)
    2. Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition. (PubMed id 22505724)1 Udeshi N.D....Carr S.A. (amp 2012)
    3. Ubiquitin-mediated proteolysis of HuR by heat shock. (PubMed id 19322201)1 Abdelmohsen K....Gorospe M. (EMBO J. 2009)
    4. The full-ORF clone resource of the German cDNA consortium. (PubMed id 17974005)2 Bechtel S.... Schupp I. (BMC Genomics 2007)
    5. Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)1 Kimura K.... Sugano S. (Genome Res. 2006)
    6. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    7. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)2 Ota T.... Sugano S. (Nat. Genet. 2004)
    8. The DNA sequence and biology of human chromosome 19. (PubMed id 15057824)2 Grimwood J.... Lucas S.M. (Nature 2004)
    9. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 125919 HGNC: 25281 AceView: ZNF543 Ensembl:ENSG00000178229 euGenes: HUgn125919
    ECgene: ZNF543 H-InvDB: ZNF543

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for ZNF543 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for ZNF543 gene:
    Search GeneIP for patents involving ZNF543

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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