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ZNF474 Gene

protein-coding   GIFtS: 40
GCID: GC05P121465

Zinc Finger Protein 474

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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Zinc Finger Protein 4741 2
Testis-Specific Zinc Finger Protein2 3
TSZFP2 3
4933409D10Rik2

External Ids:    HGNC: 232451   Entrez Gene: 1339232   Ensembl: ENSG000001641857   UniProtKB: Q6S9Z53   

Export aliases for ZNF474 gene to outside databases

Previous GC identifers: GC05P121494 GC05P116656


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for ZNF474 Gene:
ZNF474 (zinc finger protein 474) is a protein-coding gene.


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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Regulatory elements:
   Regulatory transcription factor binding sites in the ZNF474 gene promoter:
         TBP   AML1a   Brachyury   Ik-3   FOXL1   AREB6   TFIID   FOXJ2 (long isoform)   Zic1   FOXJ2   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidZNF474 promoter sequence
   Search Chromatin IP Primers for ZNF474

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat ZNF474


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 5q23.2   Ensembl cytogenetic band:  5q23.2   HGNC cytogenetic band: 5q23.2

ZNF474 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ZNF474 gene location

GeneLoc information about chromosome 5         GeneLoc Exon Structure

GeneLoc location for GC05P121465:  view genomic region     (about GC identifiers)

Start:
121,465,208 bp from pter      End:
121,515,312 bp from pter
Size:
50,105 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: ZN474_HUMAN, Q6S9Z5 (See protein sequence)
Recommended Name: Zinc finger protein 474  
Size: 364 amino acids; 40315 Da
Sequence caution: Sequence=BAB71507.1; Type=Frameshift; Positions=318;
Secondary accessions: A8K4M0 Q96M07

Explore the universe of human proteins at neXtProt for ZNF474: NX_Q6S9Z5

Explore proteomics data for ZNF474 at MOPED

Post-translational modifications: 

  • Ubiquitination2 at Lys327
  • Modification sites at PhosphoSitePlus

  • See ZNF474 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_997200.1  
    ENSEMBL proteins: 
     ENSP00000296600   ENSP00000422285   ENSP00000424794  

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    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    ZNF: Zinc fingers, C2H2-type

    2 InterPro protein domains:
     IPR026319 ZC2HC1
     IPR007087 Znf_C2H2

    Graphical View of Domain Structure for InterPro Entry Q6S9Z5

    ProtoNet protein and cluster: Q6S9Z5

    UniProtKB/Swiss-Prot: ZN474_HUMAN, Q6S9Z5
    Similarity: Contains 1 C2H2-type zinc finger


    Find genes that share domains with ZNF474           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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         Gene Ontology (GO): 1 molecular function term:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0046872metal ion binding IEA--
         
    Find genes that share ontologies with ZNF474           About GenesLikeMe


    Animal Models:
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    hsa-mir-335-5p (MIRT018912)

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    hsa-miR-577 hsa-miR-4317 hsa-miR-3673 hsa-miR-138-2* hsa-miR-10b* hsa-miR-3927 hsa-miR-514 hsa-miR-3668
    SwitchGear 3'UTR luciferase reporter plasmidZNF474 3' UTR sequence
    Inhib. RNA
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    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus3
    cytosol2
    cytoskeleton1

    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for ZNF474
    Interactions:

        Search GeneGlobe Interaction Network for ZNF474

    STRING Interaction Network Preview (showing 3 interactants - click image to see more details)

    3 Interacting proteins for ZNF474 (ENSP000002966004) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    UBCENSP000003448184STRING: ENSP00000344818
    UBE2IENSP000003248974STRING: ENSP00000324897
    APCENSP000002574304STRING: ENSP00000257430
    About this table

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for ZNF474 (ZN474)



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for ZNF474 gene: 
    NM_207317.1  

    4 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000296600(uc003ksv.3) ENST00000504912 ENST00000505843 ENST00000514925

    miRNA
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    Selected qRT-PCR Assays for microRNAs that regulate ZNF474 (see all 10):
    hsa-miR-577 hsa-miR-4317 hsa-miR-3673 hsa-miR-138-2* hsa-miR-10b* hsa-miR-3927 hsa-miR-514 hsa-miR-3668
    SwitchGear 3'UTR luciferase reporter plasmidZNF474 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat ZNF474
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      QuantiFast Probe-based Assays in human, mouse, rat ZNF474

    Selected AceView cDNA sequences (see all 31):

    AI217364 AA424196 AA846387 AA778547 AY461732 BX089039 AI797455 BI825704 
    NM_207317 AW304004 BX098374 AA969599 AA442327 BF056972 AI188208 AW631178 
    AI027637 AI223337 AW340809 AI150580 BG202474 BM979960 AK057483 BU567915 

    GeneLoc Exon Structure

    3 Alternative Splicing Database (ASD) splice patterns (SP) for ZNF474    About this scheme

    ExUns: 1a · 1b · 1c ^ 2 ^ 3a · 3b · 3c
    SP1:              -                           
    SP2:              -     -                     
    SP3:                    -                     


    ECgene alternative splicing isoforms for ZNF474

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    ZNF474 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CCACACAAGA
    ZNF474 Expression
    About this image

    ZNF474 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    ZNF474 Protein Expression
        Custom PCR Arrays for ZNF474
    Primer
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ZNF474

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals.

    Orthologs for ZNF474 gene from Selected species (see all 10)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Zfp4741 , 5 zinc finger protein 4741, 5 77.07(n)1
    70.55(a)1
      18 (28.24 cM)5
    667581  NM_025749.31  NP_080025.31 
     526159155 
    lizard
    (Anolis carolinensis)
    Reptilia ZNF4746
    zinc finger protein 474
    35(a)
    1 ↔ 1
    GL343193.1(11785837-11805835)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia LOC1004948411 zinc finger protein 474-like 55.56(n)
    54.3(a)
      100494841  XM_004910484.1  XP_004910541.1 
    worm
    (Caenorhabditis elegans)
    Secernentea M153.41 M153.4 56.16(n)
    56.52(a)
      3565375  NM_001029637.1  NP_001024808.1 


    ENSEMBL Gene Tree for ZNF474 (if available)
    TreeFam Gene Tree for ZNF474 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for ZNF474 gene

    Find genes that share paralogs with ZNF474           About GenesLikeMe


    1 Pseudogenes.org Pseudogene for ZNF474
    PGOHUM00000235744


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for ZNF474 (see all 594)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 5 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs171488651,2
    C,F,H--121463284(+) TGTATC/TGTATG 1 -- us2k112Minor allele frequency- T:0.06NA NS EA CSA WA 1342
    rs1462676351,2
    --121463351(+) AGTTAA/GAGCGT 1 -- us2k10--------
    rs1141271681,2
    F--121463355(+) AGAGCG/ATTATT 1 -- us2k11Minor allele frequency- A:0.01WA 118
    rs784660341,2
    --121463390(+) TGTCCC/TTTGGG 1 -- us2k10--------
    rs1494619301,2
    C--121463518(+) GTCAAA/GTTTTT 1 -- us2k10--------
    rs2005024091,2
    C--121463545(-) GTTAGA/CAAAAA 1 -- us2k10--------
    rs1438508951,2
    --121463560(+) AAGGAA/GCATTC 1 -- us2k10--------
    rs1828864111,2
    --121463795(+) CAGAAC/TATTTA 1 -- us2k10--------
    rs1486392031,2
    --121463822(+) ACAATA/TATCTT 1 -- us2k10--------
    rs780214961,2
    F--121463855(+) TACGAA/GTCTTG 1 -- us2k11Minor allele frequency- G:0.03WA 118

    HapMap Linkage Disequilibrium report for ZNF474 (121465208 - 121515312 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 1 variation for ZNF474:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv272075CNV Insertion20981092

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing ZNF474
    DNA2.0 Custom Variant and Variant Library Synthesis for ZNF474

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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      --

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for ZNF474 gene integrated from 10 sources:
    (articles sorted by number of sources associating them with ZNF474)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    2. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (Nat. Genet. 2004)
    3. A data set of human endogenous protein ubiquitination sites. (PubMed id 20972266)1 Shi Y....Qin J. (amp 2011)
    4. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)
    5. (PubMed id )2 

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 133923 HGNC: 23245 AceView: FLJ32921 Ensembl:ENSG00000164185 euGenes: HUgn133923
    ECgene: ZNF474 H-InvDB: ZNF474

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for ZNF474 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for ZNF474 gene:
    Search GeneIP for patents involving ZNF474

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014 , 7 Oct 2014

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