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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

ZNF474 Gene

protein-coding   GIFtS: 41
GCID: GC05P121465

Zinc Finger Protein 474

  Search for ZNF474
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

Aliases
Zinc Finger Protein 4741 2
Testis-Specific Zinc Finger Protein2 3
TSZFP2 3
4933409D10Rik2

External Ids:    HGNC: 232451   Entrez Gene: 1339232   Ensembl: ENSG000001641857   UniProtKB: Q6S9Z53   

Export aliases for ZNF474 gene to outside databases

Previous GC identifers: GC05P121494 GC05P116656


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

GeneCards Summary for ZNF474 Gene: 
ZNF474 (zinc finger protein 474) is a protein-coding gene. GO annotations related to this gene include zinc ion binding.


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 73), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000005.9  NC_018916.2  NT_034772.6  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the ZNF474 gene promoter:
         TBP   AML1a   Brachyury   Ik-3   FOXL1   AREB6   TFIID   FOXJ2 (long isoform)   Zic1   FOXJ2   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidZNF474 promoter sequence
   Search SABiosciences Chromatin IP Primers for ZNF474

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ZNF474


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 5q23.2   Ensembl cytogenetic band:  5q23.2   HGNC cytogenetic band: 5q23.2

ZNF474 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ZNF474 gene location

GeneLoc information about chromosome 5         GeneLoc Exon Structure

GeneLoc location for GC05P121465:  view genomic region     (about GC identifiers)

Start:
121,465,208 bp from pter      End:
121,515,312 bp from pter
Size:
50,105 bases      Orientation:
plus strand

(According to UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MAXQB RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Ontologies according to Gene Ontology Consortium 01 Oct 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

UniProtKB/Swiss-Prot: ZN474_HUMAN, Q6S9Z5 (See protein sequence)
Recommended Name: Zinc finger protein 474  
Size: 364 amino acids; 40315 Da
Sequence caution: Sequence=BAB71507.1; Type=Frameshift; Positions=318;
Secondary accessions: A8K4M0 Q96M07

Explore the universe of human proteins at neXtProt for ZNF474: NX_Q6S9Z5

Explore proteomics data for ZNF474 at MOPED 

Post-translational modifications:

  • View modification sites using PhosphoSitePlus
  • View neXtProt modification sites for NX_Q6S9Z5

  • ZNF474 Protein expression data from MOPED1, PaxDb2 and MAXQB3 :    About this image 

    ZNF474 Protein Expression
    REFSEQ proteins: NP_997200.1  
    ENSEMBL proteins: 
     ENSP00000296600   ENSP00000422285   ENSP00000424794  

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    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    HGNC Gene Families: 
    ZNF: Zinc fingers, C2H2-type

    2 InterPro protein domains:
     IPR026319 ZC2HC1
     IPR007087 Znf_C2H2

    Graphical View of Domain Structure for InterPro Entry Q6S9Z5

    ProtoNet protein and cluster: Q6S9Z5

    UniProtKB/Swiss-Prot: ZN474_HUMAN, Q6S9Z5
    Similarity: Contains 1 C2H2-type zinc finger


    ZNF474 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, Vector BioLabs, and Sirion Biotech, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene.)
    About This Section

         Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0008270zinc ion binding ----
    GO:0046872metal ion binding IEA--
         
    ZNF474 for ontologies           About GeneDecksing


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    SwitchGear 3'UTR luciferase reporter plasmidZNF474 3' UTR sequence
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    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for ZNF474

    STRING Interaction Network Preview (showing 3 interactants - click image to see more details)

    3 Interacting proteins for ZNF474 (ENSP000002966004) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    UBCENSP000003448184STRING: ENSP00000344818
    UBE2IENSP000003248974STRING: ENSP00000324897
    APCENSP000002574304STRING: ENSP00000257430
    About this table

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for ZNF474 (ZN474)

    Search CenterWatch for drugs/clinical trials and news about ZNF474 / ZN474

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
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    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Sirion Biotech, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for ZNF474 gene: 
    NM_207317.1  

    Unigene Cluster for ZNF474:

    Zinc finger protein 474
    Hs.729673  [show with all ESTs]
    Unigene Representative Sequence: AY461732
    4 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000296600(uc003ksv.3) ENST00000504912 ENST00000505843 ENST00000514925

    miRNA
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    hsa-miR-577 hsa-miR-4317 hsa-miR-3673 hsa-miR-138-2* hsa-miR-10b* hsa-miR-3927 hsa-miR-514 hsa-miR-3668
    SwitchGear 3'UTR luciferase reporter plasmidZNF474 3' UTR sequence
    Inhib. RNA
    Products:
         
    Browse for Gene Knock-down Tools from EMD Millipore
    OriGene RNAi products in human, mouse, rat for ZNF474
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      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat ZNF474

    Additional mRNA sequence: 

    AK057483.1 AK290985.1 AY461732.1 BC133022.1 BC133026.1 

    2 DOTS entries:

    DT.99986435  DT.91746775 

    24/31 AceView cDNA sequences (see all 31):

    AI797455 AY461732 NM_207317 AA846387 AI217364 BX089039 AA424196 AA778547 
    BI825704 AI027637 AA442327 AI223337 BG202474 BX098374 AW340809 AW631178 
    AW304004 AA969599 AI150580 BF056972 BM979960 AK057483 AI188208 AI123441 

    GeneLoc Exon Structure

    3 Alternative Splicing Database (ASD) splice patterns (SP) for ZNF474    About this scheme

    ExUns: 1a · 1b · 1c ^ 2 ^ 3a · 3b · 3c
    SP1:              -                           
    SP2:              -     -                     
    SP3:                    -                     


    ECgene alternative splicing isoforms for ZNF474

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    ZNF474 expression in normal human tissues (normalized intensities)      ZNF474 embryonic expression: see
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CCACACAAGA
    ZNF474 Expression
    About this image


    ZNF474 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database 
     5/2 selected tissues (see all 2) fully expand
     
     Epithelium (Respiratory System)    fully expand to see all 2 entries
             bronchial epithelial cells   
     
     Pancreas (Endocrine System)
             Posterior foregut-like cells ( A scalable, suspension protocol for derivation of...

    See ZNF474 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for ZNF474

    SOURCE GeneReport for Unigene cluster: Hs.729673
        SABiosciences Custom PCR Arrays for ZNF474
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ZNF474

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for ZNF474 gene from 3/9 species (see all 9)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Zfp4741 , 5 zinc finger protein 4741, 5 78.13(n)1
    71.14(a)1
      18 (28.24 cM)5
    667581  NM_025749.31  NP_080025.31 
     526159155 
    lizard
    (Anolis carolinensis)
    Reptilia ZNF4746
    Uncharacterized protein
    30(a)
    1 ↔ 1
    GL343193.1(11785837-11805835)
    worm
    (Caenorhabditis elegans)
    Secernentea M153.41 Protein M153.4 51.85(n)
    49.57(a)
      3565375  NM_001029637.1  NP_001024808.1 


    ENSEMBL Gene Tree for ZNF474 (if available)
    TreeFam Gene Tree for ZNF474 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for ZNF474 gene

    ZNF474 for paralogs           About GeneDecksing


    1 Pseudogenes.org Pseudogene for ZNF474
    PGOHUM00000235744


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/594 SNPs in ZNF474 are shown (see all 594)    About this table     
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 5 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs171488651,2
    C,F,H--121463284(+) TGTATC/TGTATG 1 -- us2k112Minor allele frequency- T:0.06NA NS EA CSA WA 1342
    rs1462676351,2
    --121463351(+) AGTTAA/GAGCGT 1 -- us2k10--------
    rs1141271681,2
    F--121463355(+) AGAGCG/ATTATT 1 -- us2k11Minor allele frequency- A:0.01WA 118
    rs784660341,2
    --121463390(+) TGTCCC/TTTGGG 1 -- us2k10--------
    rs1494619301,2
    C--121463518(+) GTCAAA/GTTTTT 1 -- us2k10--------
    rs2005024091,2
    C--121463545(-) GTTAGA/CAAAAA 1 -- us2k10--------
    rs1438508951,2
    --121463560(+) AAGGAA/GCATTC 1 -- us2k10--------
    rs1828864111,2
    --121463795(+) CAGAAC/TATTTA 1 -- us2k10--------
    rs1486392031,2
    --121463822(+) ACAATA/TATCTT 1 -- us2k10--------
    rs780214961,2
    F--121463855(+) TACGAA/GTCTTG 1 -- us2k11Minor allele frequency- G:0.03WA 118

    HapMap Linkage Disequilibrium report for ZNF474 (121465208 - 121515312 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 1 variation for ZNF474:    About this table     
    Variant IDTypeSubtypePubMed ID
    esv272075CNV Insertion20981092

    SABiosciences Cancer Mutation PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
      --

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for ZNF474 gene integrated from 9 sources:
    (articles sorted by number of sources associating them with ZNF474)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    2. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    3. A data set of human endogenous protein ubiquitination sites. (PubMed id 20972266)1 Shi Y....Qin J. (2011)
    4. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
    5. Hepatitis C virus core protein interacts with 14-3-3 protein and activates the kinase Raf-1. (PubMed id )2 Aoki H.... Hino O. (2000)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 133923 HGNC: 23245 AceView: FLJ32921 Ensembl:ENSG00000164185 euGenes: HUgn133923
    ECgene: ZNF474 H-InvDB: ZNF474

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for ZNF474 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for ZNF474 gene:
    Search GeneIP for patents involving ZNF474

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Sirion Biotech, Cell lines from GenScript, and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

     
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    GeneCards Homepage - Last full update: 23 Oct 2013 - Incrementals: 3 Nov 2013 , 7 Nov 2013 , 23 Jan 2014

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    (GIFtS: 73)
    transforming growth factor, beta 1
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


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