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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

ZNF474 Gene

protein-coding GIFtS: 40
GCID: GC05P121465

zinc finger protein 474

Search for ZNF474
in

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Zinc Finger Protein 474¹ ²
4933409D10Rik¹ ²
Testis-Specific Zinc Finger Protein² ³
TSZFP² ³
FLJ32921¹

External Ids:	HGNC: 23245¹	Entrez Gene: 133923²	Ensembl: ENSG00000164185⁷	UniProtKB: Q6S9Z5³

Export aliases for ZNF474 gene to outside databases

Previous GC identifers: GC05P121494 GC05P116656

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000005.9 NC_018916.1 NT_034772.6

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the ZNF474 gene promoter:
TBP AML1a Brachyury Ik-3 FOXL1 AREB6 TFIID FOXJ2 (long isoform) Zic1 FOXJ2
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: ZNF474 promoter sequence

Search SABiosciences Chromatin IP Primers for ZNF474

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ZNF474

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 5q23.2 Ensembl cytogenetic band: 5q23.2 HGNC cytogenetic band: 5q23.2

ZNF474 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ZNF474 gene location

GeneLoc information about chromosome 5 GeneLoc Exon Structure

GeneLoc location for GC05P121465: view genomic region (about GC identifiers)

Start:	121,465,208 bp from pter	End:	121,515,312 bp from pter
Size:	50,105 bases	Orientation:	plus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: ZN474_HUMAN, Q6S9Z5 (See protein sequence)

Recommended Name: Zinc finger protein 474

Size: 364 amino acids; 40315 Da

Sequence caution: Sequence=BAB71507.1; Type=Frameshift; Positions=318;

Secondary accessions: A8K4M0 Q96M07

Explore the universe of human proteins at neXtProt for ZNF474: NX_Q6S9Z5

Post-translational modifications:

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_Q6S9Z5

ZNF474 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins: NP_997200.1

ENSEMBL proteins:

ENSP00000296600 ENSP00000422285 ENSP00000424794

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Gene Ontology (GO): 1 cellular component term (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005622	intracellular	--	--

ZNF474 for ontologies About GeneDecksing

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(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

ZNF474 for domains About GeneDecksing

2 InterPro domains/families:

IPR026319 ZC2HC1

IPR007087 Znf_C2H2

Graphical View of Domain Structure for InterPro Entry Q6S9Z5

ProtoNet protein and cluster: Q6S9Z5

UniProtKB/Swiss-Prot: ZN474_HUMAN, Q6S9Z5

Similarity: Contains 1 C2H2-type zinc finger

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Gene Ontology (GO): 1 molecular function term (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0008270	zinc ion binding	IEA	--

ZNF474 for ontologies About GeneDecksing

Animal Models:

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In Situ Assay
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(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Interactions:

Search SABiosciences Gene Network Central^TM Interacting Genes and Proteins Networks for ZNF474

STRING Interaction Network Preview (showing 3 interactants - click image to see more details)

3 Interacting proteins for ZNF474 (ENSP00000296600⁴) via UniProtKB, MINT, STRING, and/or I2D

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
UBC	ENSP00000344818⁴	STRING: ENSP00000344818
UBE2I	ENSP00000324897⁴	STRING: ENSP00000324897
APC	ENSP00000257430⁴	STRING: ENSP00000257430

About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

Browse Small Molecules at EMD Millipore

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Browse Tocris compounds for ZNF474
Search CenterWatch for drugs/clinical trials and news about ZNF474 / ZN474

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
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About This Section

REFSEQ mRNAs for ZNF474 gene:

NM_207317.1

Unigene Cluster for ZNF474:

Zinc finger protein 474

Hs.729673 [show with all ESTs]

Unigene Representative Sequence: AY461732

4 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000296600(uc003ksv.3) ENST00000504912 ENST00000505843 ENST00000514925

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		SwitchGear 3'UTR luciferase reporter plasmid: ZNF474 3' UTR sequence

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Additional cDNA sequence:

AK057483.1 AK290985.1 AY461732.1 BC133022.1 BC133026.1

2 DOTS entries:

DT.99986435 DT.91746775

24/31 AceView cDNA sequences (see all 31):

AA778547 BI825704 AA424196 AY461732 AI217364 AA846387 NM_207317 BX089039

AI797455 AI027637 BM979960 AW304004 BG202474 BF056972 AI150580 AW340809

AK057483 AW631178 AA969599 AI188208 AI223337 BX098374 AA442327 BU567915

GeneLoc Exon Structure

3 Alternative Splicing Database (ASD) splice patterns (SP) for ZNF474 About this scheme

ExUns:	1a	·	1b	·	1c	^	2	^	3a	·	3b	·	3c
SP1:					-
SP2:					-		-
SP3:							-

ECgene alternative splicing isoforms for ZNF474

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

ZNF474 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: CCACACAAGA

About this image

ZNF474 expression in embryonic tissues and stem cells
Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

Stem Cell Differentiation: 1 LifeMap Cell

Name

Category

Posterior foregut-like cells (A scalable, suspensi...)

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

See ZNF474 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for ZNF474

SOURCE GeneReport for Unigene cluster: Hs.729673

SABiosciences Custom PCR Arrays for ZNF474

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In Situ
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(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of animals.

Orthologs for ZNF474 gene from 5/15 species (see all 15) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Zfp474^{1 , 5}	zinc finger protein 474^{1, 5}	78.13(n)¹ 71.14(a)¹		18 (28.24 cM)⁵ 66758¹ NM_025749.3¹ NP_080025.3¹ 52615915⁵
chicken (Gallus gallus)	Aves	ZNF474⁶	Uncharacterized protein	39(a)	1 ↔ 1	Z(73661452-73664941)
lizard (Anolis carolinensis)	Reptilia	ZNF474⁶	--	56(a)	1 ↔ 1	GL343193.1(11793453-11804902)
honey bee (Apis mellifera)	Insecta	--	--	24(a)	1 ↔ 1	Group5.12(597052-602373)
worm (Caenorhabditis elegans)	Secernentea	M153.4¹	Protein M153.4	51.85(n) 49.57(a)		3565375 NM_001029637.1 NP_001024808.1

ENSEMBL Gene Tree for ZNF474 (if available)
TreeFam Gene Tree for ZNF474 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

Paralogs for ZNF474 gene

ZNF474 for paralogs About GeneDecksing

1 Pseudogenes.org Pseudogene for ZNF474
PGOHUM00000235744

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 5 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs17148865^1,2	C,F,H	--	121463284(+)	`TGTATC/TGTATG`	1	--	us2k¹		12		NA NS EA CSA WA	1342
rs146267635^1,2		--	121463351(+)	`AGTTAA/GAGCGT`	1	--	us2k¹		0	--	--	--	--
rs114127168^1,2		--	121463355(+)	`AGAGCG/ATTATT`	1	--	us2k¹		1		WA	118
rs78466034^1,2		--	121463390(+)	`TGTCCC/TTTGGG`	1	--	us2k¹		0	--	--	--	--
rs149461930^1,2		--	121463518(+)	`GTCAAA/GTTTTT`	1	--	us2k¹		0	--	--	--	--
rs200502409^1,2	C	--	121463545(-)	`GTTAGA/CAAAAA`	1	--	us2k¹		0	--	--	--	--
rs143850895^1,2		--	121463560(+)	`AAGGAA/GCATTC`	1	--	us2k¹		0	--	--	--	--
rs182886411^1,2		--	121463795(+)	`CAGAAC/TATTTA`	1	--	us2k¹		0	--	--	--	--
rs148639203^1,2		--	121463822(+)	`ACAATA/TATCTT`	1	--	us2k¹		0	--	--	--	--
rs78021496^1,2	F	--	121463855(+)	`TACGAA/GTCTTG`	1	--	us2k¹		1		WA	118

HapMap Linkage Disequilibrium report for ZNF474 (121465208 - 121515312 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for ZNF474: --

SABiosciences Cancer Mutation PCR Assays

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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for ZNF474 gene integrated from 9 sources:
(articles sorted by number of sources associating them with ZNF474)

Utopia: connect your pdf to the dynamic
world of online information

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{1, 2} Ota T.... Sugano S. (2004)
A data set of human endogenous protein ubiquitination sites. (PubMed id 20972266)¹ Shi Y....Qin J. (2011)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)¹ Strausberg R.L....Marra M.A. (2002)
Hepatitis C virus core protein interacts with 14-3-3 protein and activates the kinase Raf-1. (PubMed id )² Aoki H.... Hino O. (2000)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 133923	HGNC: 23245	AceView: FLJ32921	Ensembl:ENSG00000164185	euGenes: HUgn133923
ECgene: ZNF474	H-InvDB: ZNF474

(According to HUGE)
About This Section

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for ZNF474	Pharmacogenomics, SNPs, Pathways

Intellectual Property for ZNF474 gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

About This Section

Patent Information for ZNF474 gene:
Search GeneIP for patents involving ZNF474

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, LifeMap BioReagents, and Sirion Biotech, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory)
About This Section


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GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013 , 13 May 2013

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Category			VWF (GIFTS: 73) von Willebrand factor
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The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

The GeneCards Human Gene Database

Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

Hot genes Disease genes ZNF474 gene at Home site.

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ZNF474 Gene

zinc finger protein 474

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ZNF474 Gene

zinc finger protein 474

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