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ZNF461 Gene

protein-coding   GIFtS: 51
GCID: GC19M037128

Zinc Finger Protein 461

  Search for ZNF461
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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This gene clusters with an RNA gene
Subcategory (RNA class): lncRNA

Quality score for the ORGUL clustered with this gene is 3

Aliases
Zinc Finger Protein 4611 2
GIOT12 3 5
Gonadotropin-Inducible Ovary Transcription Repressor 12 3
GIOT-12 3
Gonadotropin Inducible Transcription Repressor 12

External Ids:    HGNC: 216291   Entrez Gene: 922832   Ensembl: ENSG000001978087   OMIM: 6086405   UniProtKB: Q8TAF73   
ORGUL members:         

Export aliases for ZNF461 gene to outside databases

Previous GC identifers: GC19M041822 GC19M033596


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for ZNF461 Gene:
ZNF461 (zinc finger protein 461) is a protein-coding gene. An important paralog of this gene is ZNF79.

UniProtKB/Swiss-Prot: ZN461_HUMAN, Q8TAF7
Function: May be involved in transcriptional regulation




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000019.9  NT_011109.17  NC_018930.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the ZNF461 gene promoter:
         C/EBPbeta   RP58   Ik-3   Ik-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidZNF461 promoter sequence
   Search Chromatin IP Primers for ZNF461

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat ZNF461


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 19q13.12   Ensembl cytogenetic band:  19q13.12   HGNC cytogenetic band: 19q13.13

ZNF461 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ZNF461 gene location

GeneLoc information about chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19M037128:  view genomic region     (about GC identifiers)

Start:
37,128,094 bp from pter      End:
37,157,755 bp from pter
Size:
29,662 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: ZN461_HUMAN, Q8TAF7 (See protein sequence)
Recommended Name: Zinc finger protein 461  
Size: 563 amino acids; 66214 Da
Secondary accessions: A8K9W9 Q6VSF7 Q9ULZ8
Alternative splicing: 2 isoforms:  Q8TAF7-1   Q8TAF7-2   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for ZNF461: NX_Q8TAF7

Explore proteomics data for ZNF461 at MOPED

Post-translational modifications: 

  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See ZNF461 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_694989.2  
    ENSEMBL proteins: 
     ENSP00000380102   ENSP00000467931   ENSP00000353515   ENSP00000468822   ENSP00000465235  
     ENSP00000465668   ENSP00000464914  
    Reactome Protein details: Q8TAF7

    ZNF461 Human Recombinant Protein Products:

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    Cloud-Clone Corp. Proteins for ZNF461

     
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    Search eBioscience for ELISAs for ZNF461 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    ZNF: Zinc fingers, C2H2-type

    4 InterPro protein domains:
     IPR001909 Krueppel-associated_box
     IPR015880 Znf_C2H2-like
     IPR013087 Znf_C2H2/integrase_DNA-bd
     IPR007087 Znf_C2H2

    Graphical View of Domain Structure for InterPro Entry Q8TAF7

    ProtoNet protein and cluster: Q8TAF7

    2 Blocks protein domains:
    IPB001909 KRAB box
    IPB007086 C2H2-type zinc finger signature


    UniProtKB/Swiss-Prot: ZN461_HUMAN, Q8TAF7
    Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family
    Similarity: Contains 12 C2H2-type zinc fingers
    Similarity: Contains 1 KRAB domain


    Find genes that share domains with ZNF461           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: ZN461_HUMAN, Q8TAF7
    Function: May be involved in transcriptional regulation

         Gene Ontology (GO): 4 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003676nucleic acid binding ----
    GO:0003677DNA binding IEA--
    GO:0003700sequence-specific DNA binding transcription factor activity IBA--
    GO:0046872metal ion binding IEA--
         
    Find genes that share ontologies with ZNF461           About GenesLikeMe


    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for ZNF461
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    miRNA
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    miRTarBase miRNAs that target ZNF461:
    hsa-mir-335-5p (MIRT017024)

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    3 qRT-PCR Assays for microRNAs that regulate ZNF461:
    hsa-miR-579 hsa-miR-4276 hsa-miR-29b-1*
    SwitchGear 3'UTR luciferase reporter plasmidZNF461 3' UTR sequence
    Inhib. RNA
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ZNF461


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    ZN461_HUMAN, Q8TAF7: Nucleus (Probable)
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus4
    cytosol2

    Gene Ontology (GO): 2 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005622intracellular ----
    GO:0005634nucleus IEA--

    Find genes that share ontologies with ZNF461           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for ZNF461 About    
    See pathways by source

    SuperPathContained pathways About
    1Gene Expression
    Generic Transcription Pathway0.40
    Gene Expression0.40


    Find genes that share SuperPaths with ZNF461           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 Reactome Pathway for ZNF461
        Generic Transcription Pathway


        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for ZNF461
    Interactions:

        Search GeneGlobe Interaction Network for ZNF461

    STRING Interaction Network Preview (showing 3 interactants - click image to see more details)

    4 Interacting proteins for ZNF461 (Q8TAF73 ENSP000003535154) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HDAC2Q927693, ENSP000003813314I2D: score=1 STRING: ENSP00000381331
    SF1Q156373, ENSP000003666074I2D: score=1 STRING: ENSP00000366607
    TOX2ENSP000003447244STRING: ENSP00000344724
    --G3XAC73I2D: score=1 
    About this table

    Gene Ontology (GO): 2 biological process terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006351transcription, DNA-templated IEA--
    GO:0006355regulation of transcription, DNA-templated IBA--

    Find genes that share ontologies with ZNF461           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for ZNF461 (ZN461)



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for ZNF461 gene: 
    NM_153257.2  

    Unigene Cluster for ZNF461:

    Zinc finger protein 461
    Hs.590972  [show with all ESTs]
    Unigene Representative Sequence: BX649031
    12 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000396893(uc002oem.3 uc002oen.3 uc010xtj.2) ENST00000589442
    ENST00000588268 ENST00000360357 ENST00000590487 ENST00000540605 ENST00000591370
    ENST00000590361 ENST00000588844 ENST00000591827 ENST00000592829 ENST00000592496

    miRNA
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    3 qRT-PCR Assays for microRNAs that regulate ZNF461:
    hsa-miR-579 hsa-miR-4276 hsa-miR-29b-1*
    SwitchGear 3'UTR luciferase reporter plasmidZNF461 3' UTR sequence
    Inhib. RNA
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    GenScript: all cDNA clones in your preferred vector: ZNF461 (NM_153257)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for ZNF461
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ZNF461
    Primer
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    OriGene qSTAR qPCR primer pairs in human, mouse for ZNF461
    Pre-validated RT2 qPCR Primer Assay in human, mouse / rat ZNF461
      QuantiTect SYBR Green Assays in human, mouse, rat ZNF461
      QuantiFast Probe-based Assays in human, mouse, rat ZNF461

    Additional mRNA sequence: 

    AB021641.1 AB209279.1 AK292834.1 AK299370.1 AY329493.1 AY500360.1 BC028631.1 BX649031.1 

    9 DOTS entries:

    DT.91754830  DT.101966703  DT.430801  DT.102822779  DT.95362977  DT.40206333  DT.91897672  DT.95296094 
    DT.209776 

    Selected AceView cDNA sequences (see all 27):

    BG288843 AW079205 BM713670 AY329493 AI399892 BC028631 AW974543 BI460349 
    BI552797 AB021641 BX649031 AA326608 NM_153257 AA027946 AI264877 BG434840 
    AY500360 BI464798 BI545486 BX114320 BF541218 AA027962 AA213612 AW304212 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    ZNF461 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: AAATTTGTTG
    ZNF461 Expression
    About this image

    ZNF461 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    ZNF461 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.590972

    UniProtKB/Swiss-Prot: ZN461_HUMAN, Q8TAF7
    Tissue specificity: Widely expressed, with highest levels in liver, kidney, pancreas, thymus, and small intestine

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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ZNF461

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of mammals.

    Orthologs for ZNF461 gene from Selected species (see all 2)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    cow
    (Bos taurus)
    Mammalia ZNF4611 zinc finger protein 461 81.79(n)
    73.36(a)
      100296502  XM_002694949.3  XP_002694995.3 


    ENSEMBL Gene Tree for ZNF461 (if available)
    TreeFam Gene Tree for ZNF461 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for ZNF461 gene
    ZNF792  ZNF5702  ZFP302  ZNF5292  ZNF286B2  ZNF7902  ZNF3832  ZNF1812  
    ZNF2872  ZNF5822  ZNF4712  ZFP822  ZNF286A2  ZNF2502  ZNF8792  ZNF354C2  
    ZNF742  ZNF5832  ZNF3912  ZNF5542  ZNF4542  ZFP902  ENSG000001876072  ZFP142  
    ZNF3312  ZNF354A2  ZNF354B2  ZNF342  ZNF5272  ZNF5652  
    Selected SIMAP similar genes for ZNF461 using alignment to 6 protein entries:     ZN461_HUMAN (see all proteins) (see all similar genes):
    ATP6V0A1    DNMT1    FAM210A    CHID1    FIK    CTSS
    NF2    NSRP1    ZNF78L1    ZNF83    ZNF320    ZNF738
    ZNF91    ZNF790    ZFP90    ZNF283    ZFP30    MGC12518

    Find genes that share paralogs with ZNF461           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for ZNF461 (see all 541)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 19 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1897925431,2
    --37127799(+) TTCCAA/GTGCTT 1 -- ds50010--------
    rs1123243071,2
    C--37127828(+) CTTGAA/GCACAG 1 -- ds50010--------
    rs795699551,2
    C,F--37127876(+) AGGAAC/TGTGAT 1 -- ds50011Minor allele frequency- T:0.02NA 120
    rs1826796871,2
    --37127995(+) ACAGTC/TCTACC 1 -- ds50010--------
    rs1457756461,2
    --37128029(+) TATTCA/GCTAAA 1 -- ds50010--------
    rs18805631,2
    C,F,H--37128075(-) ACCAAT/CAGATA 1 -- ds500119Minor allele frequency- C:0.03NS EA NA WA 1954
    rs1880714221,2
    --37128159(+) AGTGGC/TGCGAT 1 -- ds50010--------
    rs1928217471,2
    --37128162(+) GGCGCG/TATCTC 1 -- ds50010--------
    rs1834753541,2
    C--37128344(+) TGATCC/TGCCCG 1 -- ut310--------
    rs1387052781,2
    --37128419(+) TTTCAA/TCCACA 1 -- ut310--------

    HapMap Linkage Disequilibrium report for ZNF461 (37128094 - 37157755 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 5 variations for ZNF461:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv2475CNV Insertion18451855
    esv34384CNV Loss17911159
    nsv817833CNV Loss17921354
    nsv833817CNV Loss17160897
    nsv469794CNV Complex16826518

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing ZNF461
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 608640    OMIM disorders: --


    Find genes that share disorders with ZNF461           About GenesLikeMe

    Genetic Association Database (GAD): ZNF461

    Export disorders for ZNF461 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for ZNF461 gene integrated from 10 sources:
    (articles sorted by number of sources associating them with ZNF461)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Characterization of two novel KRAB-domain-containing zinc finger genes, ZNF460 and ZNF461, on human chromosome 19q13.1-q13.4. (PubMed id 15004467)1, 2, 3, 9 Dai J.... Mao Y. (Cytogenet. Genome Res. 2003)
    2. Cloning and characterization of gonadotropin-inducible ovarian transcription factors (GIOT1 and -2) that are novel members of the (Cys)(2)-(His)(2)-type zinc finger protein family. (PubMed id 11579202)1, 3, 9 Mizutani T....Miyamoto K. (Mol. Endocrinol. 2001)
    3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    4. Pathway Analysis of ChIP-Seq-Based NRF1 Target Genes Suggests a Logical Hypothesis of their Involvement in the Pathogenesis of Neurodegenerative Diseases. (PubMed id 24250222)1 Satoh J....Yamamoto Y. (Gene Regul Syst Bio 2013)
    5. Orphan nuclear receptor Nur77 induces zinc finger protein GIOT-1 gene expression, and GIOT-1 acts as a novel corepressor of orphan nuclear receptor SF-1 via recruitment of HDAC2. (PubMed id 16595694)1 Song K.H....Choi H.S. (J. Biol. Chem. 2006)
    6. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)2 Ota T.... Sugano S. (Nat. Genet. 2004)
    7. Cloning and characterization of granulosa cell high-mobility group (HMG)-box protein-1, a novel HMG-box transcriptional regulator strongly expressed in rat ovarian granulosa cells. (PubMed id 14764631)1 Kajitani T....Miyamoto K. (Endocrinology 2004)
    8. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)
    9. Mono-(2-ethylhexyl) phthalate induces NR4A subfamily and GIOT-1 gene expression, and suppresses CYP19 expression in human granulosa-like tumor cell line KGN. (PubMed id 19822197)9 Ohno S....Nakajin S. (Toxicol. Lett. 2009)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 92283 HGNC: 21629 AceView: GIOT-1 Ensembl:ENSG00000197808 euGenes: HUgn92283
    ECgene: ZNF461 H-InvDB: ZNF461

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for ZNF461 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for ZNF461 gene:
    Search GeneIP for patents involving ZNF461

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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