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Aliases for ZNF304 Gene

Aliases for ZNF304 Gene

  • Zinc Finger Protein 304 2 3 5
  • KRAB-Containing Zinc Finger Protein 4

External Ids for ZNF304 Gene

Previous GeneCards Identifiers for ZNF304 Gene

  • GC19P058761
  • GC19P058316
  • GC19P062538
  • GC19P062554
  • GC19P057862
  • GC19P054176

Summaries for ZNF304 Gene

Entrez Gene Summary for ZNF304 Gene

  • This gene encodes a member of the Krueppel C2H2-type zinc-finger family of proteins. The encoded protein functions as a transcriptional repressor that recruits a corepressor complex to stimulate promoter hypermethylation and transcriptional silencing of target genes. Expression of this gene is upregulated in colorectal, ovarian and breast cancer, and this gene may promote cancer cell survival, growth and invasion. [provided by RefSeq, Jul 2016]

GeneCards Summary for ZNF304 Gene

ZNF304 (Zinc Finger Protein 304) is a Protein Coding gene. Among its related pathways are Gene Expression. GO annotations related to this gene include nucleic acid binding. An important paralog of this gene is ZNF132.

UniProtKB/Swiss-Prot for ZNF304 Gene

  • Acts as transcriptional regulator and plays a role in gene silencing (PubMed:24623306, PubMed:26081979). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of several tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) by inducing trimethylation of Lys-27 of histone H3 (H3K27me3) (PubMed:24623306) in a Polycomb group (PcG) complexes-dependent manner. Associates at promoter regions of TSGs and mediates the recruitment of the corepressor complex containing the scaffolding protein TRIM28, methyltransferase DNMT1 and histone methyltransferase SETDB1 and/or the PcG complexes at those sites (PubMed:24623306). Transcription factor involved in the metastatic cascade process by inducing cell migration and proliferation and gain resistance to anoikis of ovarian carcinoma (OC) cells via integrin-mediated signaling pathways (PubMed:26081979). Associates with the ITGB1 promoter and positively regulates beta-1 integrin transcription expression (PubMed:26081979). Promotes angiogenesis (PubMed:26081979). Promotes tumor growth (PubMed:24623306, PubMed:26081979).

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for ZNF304 Gene

Genomics for ZNF304 Gene

Regulatory Elements for ZNF304 Gene

Enhancers for ZNF304 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH19F057361 1.1 ENCODE 21.4 +11.7 11737 3.2 HDGF PKNOX1 ATF1 CREB3L1 WRNIP1 ARID4B SIN3A DMAP1 BRCA1 ZNF2 ZNF548 ZNF304 ZFP28 ZNF667 ZIM2 ZNF667-AS1 PEG3 SMIM17 ZNF805 ZNF470
GH19F057388 1.1 ENCODE 19.5 +38.8 38755 2.2 PKNOX1 WRNIP1 SIN3A DMAP1 YY1 ZNF143 FOS CEBPZ SP3 SP5 ZNF805 ZNF304 ZNF264 ZNF548 ZNF543 ZNF134 RPS5 ZNF749 ENSG00000268533 ENSG00000269533
GH19F057319 1.1 ENCODE 18.5 -30.4 -30440 2.1 HDGF PKNOX1 WRNIP1 ARID4B SIN3A DMAP1 YY1 ZNF143 SP3 SP5 ZNF304 ZNF17 TRAPPC2B ZNF547 ZNF805 AURKC ZNF550 ZNF543 RPL19P19 ZNF256
GH19F057406 0.8 ENCODE 24.7 +55.5 55473 0.2 ARID4B ZNF2 RAD21 ZNF143 THAP11 ZNF654 CEBPB REST KAT8 MIER3 ZNF304 ZNF17 PIR33606
GH19F057410 1 ENCODE 19.4 +60.2 60152 1.8 PKNOX1 ATF1 CREB3L1 ARID4B SIN3A DMAP1 ETS1 YY1 SLC30A9 GLIS2 ZNF548 ZNF805 ZNF304 ZNF264 ZNF543 ZNF134 RPS5 ZNF17 GC19P057420
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around ZNF304 on UCSC Golden Path with GeneCards custom track

Promoters for ZNF304 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000045610 356 2399 PKNOX1 ATF1 CREB3L1 ARID4B DMAP1 BRCA1 ZNF2 ETS1 YY1 ZNF143

Transcription factor binding sites by QIAGEN in the ZNF304 gene promoter:

Genomic Location for ZNF304 Gene

Chromosome:
19
Start:
57,351,245 bp from pter
End:
57,359,898 bp from pter
Size:
8,654 bases
Orientation:
Plus strand

Genomic View for ZNF304 Gene

Genes around ZNF304 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ZNF304 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ZNF304 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ZNF304 Gene

Proteins for ZNF304 Gene

  • Protein details for ZNF304 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9HCX3-ZN304_HUMAN
    Recommended name:
    Zinc finger protein 304
    Protein Accession:
    Q9HCX3

    Protein attributes for ZNF304 Gene

    Size:
    659 amino acids
    Molecular mass:
    75047 Da
    Quaternary structure:
    • Probably part of a corepressor complex containing ZNF304, TRIM28, SETDB1 and DNMT1; leading to promoter hypermethylation and transcriptional silencing (PubMed:24623306). Probably associates with Polycomb group (PcG) complexes; leading to trimethylation of Lys-27 of histone H3 (H3K27me3) (PubMed:24623306). Interacts with USP28 (PubMed:24623306).

neXtProt entry for ZNF304 Gene

Post-translational modifications for ZNF304 Gene

  • Deubiquitinated by USP28; the deubiquitination leads to the stabilization of ZNF304 from proteolytic degradation (PubMed:24623306).
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for ZNF304 Gene

Domains & Families for ZNF304 Gene

Gene Families for ZNF304 Gene

Suggested Antigen Peptide Sequences for ZNF304 Gene

Graphical View of Domain Structure for InterPro Entry

Q9HCX3

UniProtKB/Swiss-Prot:

ZN304_HUMAN :
  • Contains 1 KRAB domain.
  • Belongs to the krueppel C2H2-type zinc-finger protein family.
  • Contains 16 C2H2-type zinc fingers.
Domain:
  • Contains 1 KRAB domain.
Family:
  • Belongs to the krueppel C2H2-type zinc-finger protein family.
Similarity:
  • Contains 16 C2H2-type zinc fingers.
genes like me logo Genes that share domains with ZNF304: view

Function for ZNF304 Gene

Molecular function for ZNF304 Gene

UniProtKB/Swiss-Prot Function:
Acts as transcriptional regulator and plays a role in gene silencing (PubMed:24623306, PubMed:26081979). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of several tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) by inducing trimethylation of Lys-27 of histone H3 (H3K27me3) (PubMed:24623306) in a Polycomb group (PcG) complexes-dependent manner. Associates at promoter regions of TSGs and mediates the recruitment of the corepressor complex containing the scaffolding protein TRIM28, methyltransferase DNMT1 and histone methyltransferase SETDB1 and/or the PcG complexes at those sites (PubMed:24623306). Transcription factor involved in the metastatic cascade process by inducing cell migration and proliferation and gain resistance to anoikis of ovarian carcinoma (OC) cells via integrin-mediated signaling pathways (PubMed:26081979). Associates with the ITGB1 promoter and positively regulates beta-1 integrin transcription expression (PubMed:26081979). Promotes angiogenesis (PubMed:26081979). Promotes tumor growth (PubMed:24623306, PubMed:26081979).
UniProtKB/Swiss-Prot Induction:
Down-regulated during embryonic stem cells (ESCs) differentiation by retinoic acid treatment (PubMed:24623306).

Gene Ontology (GO) - Molecular Function for ZNF304 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000977 RNA polymerase II regulatory region sequence-specific DNA binding IBA --
GO:0003676 nucleic acid binding IEA --
GO:0003677 DNA binding IEA --
GO:0003700 transcription factor activity, sequence-specific DNA binding IBA --
GO:0005515 protein binding IPI 24623306
genes like me logo Genes that share ontologies with ZNF304: view
genes like me logo Genes that share phenotypes with ZNF304: view

Animal Model Products

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , miRNA , Transcription Factor Targets and HOMER Transcription for ZNF304 Gene

Localization for ZNF304 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ZNF304 Gene

Nucleus. Note=Associates with chromatin (PubMed:24623306). {ECO:0000269 PubMed:24623306}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for ZNF304 gene
Compartment Confidence
nucleus 5
cytosol 3

Gene Ontology (GO) - Cellular Components for ZNF304 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005622 intracellular IEA --
GO:0005634 nucleus IEA --
genes like me logo Genes that share ontologies with ZNF304: view

Pathways & Interactions for ZNF304 Gene

SuperPathways for ZNF304 Gene

SuperPathway Contained pathways
1 Gene Expression
genes like me logo Genes that share pathways with ZNF304: view

Pathways by source for ZNF304 Gene

2 Reactome pathways for ZNF304 Gene

Interacting Proteins for ZNF304 Gene

Gene Ontology (GO) - Biological Process for ZNF304 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001525 angiogenesis IEA --
GO:0006351 transcription, DNA-templated IEA --
GO:0006355 regulation of transcription, DNA-templated IEA --
GO:0007229 integrin-mediated signaling pathway IMP 26081979
GO:0007265 Ras protein signal transduction IMP 24623306
genes like me logo Genes that share ontologies with ZNF304: view

No data available for SIGNOR curated interactions for ZNF304 Gene

Transcripts for ZNF304 Gene

mRNA/cDNA for ZNF304 Gene

(6) REFSEQ mRNAs :
(3) Additional mRNA sequences :
(5) Selected AceView cDNA sequences:
(4) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for ZNF304 Gene

Zinc finger protein 304:
Representative Sequences:

Inhibitory RNA Products

Clone Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for ZNF304 Gene

No ASD Table

Relevant External Links for ZNF304 Gene

GeneLoc Exon Structure for
ZNF304
ECgene alternative splicing isoforms for
ZNF304

Expression for ZNF304 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for ZNF304 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for ZNF304 Gene

This gene is overexpressed in Peripheral blood mononuclear cells (69.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for ZNF304 Gene



Protein tissue co-expression partners for ZNF304 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of ZNF304 Gene:

ZNF304

SOURCE GeneReport for Unigene cluster for ZNF304 Gene:

Hs.287374

mRNA Expression by UniProt/SwissProt for ZNF304 Gene:

Q9HCX3-ZN304_HUMAN
Tissue specificity: Expressed in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Expressed strongly in colorectal cancers cells (CRCs) (PubMed:24623306). Expressed strongly in ovarian carcinoma (OC) tumor cell lines compared to non-transformed ovarian epithelial cells (at protein level) (PubMed:26081979). Expressed in lymphoid tissues, thyroid, adrenal gland, prostate, pancreas and skeletal muscles (PubMed:12051768).
genes like me logo Genes that share expression patterns with ZNF304: view

Primer Products

No data available for mRNA differential expression in normal tissues for ZNF304 Gene

Orthologs for ZNF304 Gene

This gene was present in the common ancestor of animals.

Orthologs for ZNF304 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia ZNF304 35
  • 89 (a)
OneToOne
dog
(Canis familiaris)
Mammalia ZNF304 34 35
  • 87.69 (n)
cow
(Bos Taurus)
Mammalia ZNF304 34 35
  • 85.25 (n)
fruit fly
(Drosophila melanogaster)
Insecta CG2052 36
  • 31 (a)
Species where no ortholog for ZNF304 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • mouse (Mus musculus)
  • oppossum (Monodelphis domestica)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rat (Rattus norvegicus)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)
  • zebrafish (Danio rerio)

Evolution for ZNF304 Gene

ENSEMBL:
Gene Tree for ZNF304 (if available)
TreeFam:
Gene Tree for ZNF304 (if available)

Paralogs for ZNF304 Gene

(534) SIMAP similar genes for ZNF304 Gene using alignment to 5 proteins:

genes like me logo Genes that share paralogs with ZNF304: view

Variants for ZNF304 Gene

Sequence variations from dbSNP and Humsavar for ZNF304 Gene

SNP ID Clin Chr 19 pos Sequence Context AA Info Type
rs193920881 Uncertain significance 57,357,269(+) CCAGA(A/T)AATTC reference, missense
rs111405312 -- 57,358,961(+) CATGA(C/T)TGCTA utr-variant-3-prime
rs111415011 -- 57,350,811(+) AGAAG(G/T)ACATT upstream-variant-2KB
rs111622428 -- 57,352,727(+) GTGTG(A/G)CTGTG intron-variant, utr-variant-5-prime
rs111932808 -- 57,356,687(+) AATCC(A/G)CAGTG reference, missense

Variation tolerance for ZNF304 Gene

Residual Variation Intolerance Score: 59.2% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.40; 54.30% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for ZNF304 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
ZNF304

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Structural Variations from Database of Genomic Variants (DGV) for ZNF304 Gene

Disorders for ZNF304 Gene

Relevant External Links for ZNF304

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
ZNF304

No disorders were found for ZNF304 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for ZNF304 Gene

Publications for ZNF304 Gene

  1. The ZNF304-integrin axis protects against anoikis in cancer. (PMID: 26081979) Aslan B. … Lopez-Berestein G. (Nat Commun 2015) 3 4 64
  2. A KRAS-directed transcriptional silencing pathway that mediates the CpG island methylator phenotype. (PMID: 24623306) Serra R.W. … Green M.R. (Elife 2014) 3 4 64
  3. Identification of a KRAB-containing zinc finger protein, ZNF304, by AU-motif-directed display method and initial characterization in lymphocyte activation. (PMID: 12051768) Sabater L. … Dominguez O. (Biochem. Biophys. Res. Commun. 2002) 3 4 64
  4. Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PMID: 16344560) Kimura K. … Sugano S. (Genome Res. 2006) 3 64
  5. The DNA sequence and biology of human chromosome 19. (PMID: 15057824) Grimwood J. … Lucas S.M. (Nature 2004) 4 64

Products for ZNF304 Gene

Sources for ZNF304 Gene

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