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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

ZNF300 Gene

protein-coding   GIFtS: 53
GCID: GC05M150254

zinc finger protein 300

 Explore 1 disease affiliated with
ZNF300 via our new
 Human Malady Compendium 
Biological research products
for ZNF300
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Zinc Finger Protein 3001 2
Kruppel-Like Zinc Finger Protein2

External Ids:    HGNC: 130911   Entrez Gene: 919752   Ensembl: ENSG000001459087   OMIM: 6124295   UniProtKB: Q96RE93   

Export aliases for ZNF300 gene to outside databases

Previous GC identifers: GC21U990107 GC05M150303 GC05M145419


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for ZNF300:
The protein encoded by this gene is a C2H2-type zinc finger DNA binding protein and likely transcriptional regulator.
The function of this protein is not yet known. Three transcript variants encoding different isoforms have been found
for this gene.(provided by RefSeq, Mar 2010)

UniProtKB/Swiss-Prot: ZN300_HUMAN, Q96RE9
Function: Has a transcriptional repressor activity

Gene Wiki entry for ZNF300


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000005.9  NC_018916.1  NT_029289.11  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the ZNF300 gene promoter:
         Pbx1a   NF-YA   HNF-1A   NF-YB   CBF-B   CBF-A   HNF-1   CP1A   NF-Y   CBF(2)   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidZNF300 promoter sequence
   Search SABiosciences Chromatin IP Primers for ZNF300

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ZNF300


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 5q33.1   Ensembl cytogenetic band:  5q33.1   HGNC cytogenetic band: 5q33.1

ZNF300 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ZNF300 gene location

GeneLoc information about chromosome 5         GeneLoc Exon Structure

GeneLoc location for GC05M150254:  view genomic region     (about GC identifiers)

Start:
150,273,954 bp from pter      End:
150,284,545 bp from pter
Size:
10,592 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: ZN300_HUMAN, Q96RE9 (See protein sequence)
Recommended Name: Zinc finger protein 300  
Size: 604 amino acids; 68743 Da
Subcellular location: Nucleus
Sequence caution: Sequence=EAW61702.1; Type=Erroneous gene model prediction;
Secondary accessions: A8MY91 B3KU35 B4DU78 Q06DQ3 Q17RP3 Q5H9N5
Alternative splicing: 3 isoforms:  Q96RE9-1   Q96RE9-2   Q96RE9-3   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for ZNF300: NX_Q96RE9

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q96RE9

  • ZNF300 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (3 alternative transcripts): 
    NP_001166302.1  NP_001166303.1  NP_443092.1  

    ENSEMBL proteins: 
     ENSP00000414195   ENSP00000377773   ENSP00000397178   ENSP00000274599   ENSP00000392593  
    Reactome Protein details: Q96RE9
    Human Recombinant Protein Products: 
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    Uscn Proteins for ZNF300

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005622intracellular ----
    GO:0005634nucleus IEA--


    ZNF300 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    ZNF300 for domains           About GeneDecksing

    4 InterPro domains/families:
     IPR015880 Znf_C2H2-like
     IPR001909 Krueppel-associated_box
     IPR013087 Znf_C2H2/integrase_DNA-bd
     IPR007087 Znf_C2H2

    Graphical View of Domain Structure for InterPro Entry Q96RE9

    ProtoNet protein and cluster: Q96RE9

    2 Blocks protein families:
    IPB001909 KRAB box
    IPB007086 C2H2-type zinc finger signature


    UniProtKB/Swiss-Prot: ZN300_HUMAN, Q96RE9
    Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family
    Similarity: Contains 12 C2H2-type zinc fingers
    Similarity: Contains 1 KRAB domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: ZN300_HUMAN, Q96RE9
    Function: Has a transcriptional repressor activity

    miRNA
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    hsa-miR-587 hsa-miR-610
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    Inhib. RNA
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    Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003676nucleic acid binding ----
    GO:0003677DNA binding IEA--
    GO:0008270zinc ion binding IEA--


    ZNF300 for ontologies           About GeneDecksing


    2 GenomeRNAi human phenotypes for ZNF300:
     Decreased viability with pacli  Increased number of cells in m 


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1Generic Transcription Pathway
    Generic Transcription Pathway1.00
    Gene Expression0.46

    Pathway sources
    See GeneCards unified pathways
    Show all pathways


    2        Reactome Pathways for ZNF300
        Generic Transcription Pathway
    Gene Expression



    ZNF300 for pathways           About GeneDecksing

    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for ZNF300

    1 Interacting protein for ZNF300 (Q96RE93) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    SPI1P179473I2D: score=1 
    About this table

    Gene Ontology (GO): 2 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006351transcription, DNA-dependent IEA--
    GO:0006355regulation of transcription, DNA-dependent IEA--


    ZNF300 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for ZNF300
    Search CenterWatch for drugs/clinical trials and news about ZNF300 / ZN300 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for ZNF300 gene (3 alternative transcripts): 
    NM_001172831.1  NM_001172832.1  NM_052860.2  

    Unigene Cluster for ZNF300:

    Zinc finger protein 300
    Hs.134885  [show with all ESTs]
    Unigene Representative Sequence: BC032607
    5 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000427179 ENST00000394226 ENST00000446148(uc021yfx.1) ENST00000274599(uc021yfy.1)
    ENST00000418587(uc021yfz.1)

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    hsa-miR-587 hsa-miR-610
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    Inhib. RNA
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    Additional cDNA sequence: 

    AF395541.1 AK026949.1 AK095642.1 AK096465.1 AK300528.1 BC032607.1 BC117248.1 CR933727.1 
    DQ908918.1 L08442.1 

    9 DOTS entries:

    DT.112297  DT.75166362  DT.102836548  DT.100021826  DT.100704706  DT.120859081  DT.95290248  DT.120859069 
    DT.70103281 

    24/71 AceView cDNA sequences (see all 71):

    BG675606 AA649651 CA441804 CR600345 AK096465 AA046814 AI915570 BX333957 
    BM473496 AA393615 AK095642 CR597788 AL711433 NM_052860 BC032607 BU194076 
    CA424810 BF222831 CA427892 CA393223 AF395541 AW631393 AK026949 AA679764 

    GeneLoc Exon Structure

    5/8 Alternative Splicing Database (ASD) splice patterns (SP) for ZNF300 (see all 8)    About this scheme

    ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b · 6c · 6d · 6e · 6f ^ 7a · 7b · 7c · 7d
    SP1:                          -           -     -                                                         
    SP2:                          -                 -                                                         
    SP3:                          -           -     -     -                                                   
    SP4:                          -     -     -     -                                                         
    SP5:                                                                                                      


    ECgene alternative splicing isoforms for ZNF300

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    ZNF300 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: TAAAGTTCTT

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See ZNF300 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for ZNF300

    SOURCE GeneReport for Unigene cluster: Hs.134885

    UniProtKB/Swiss-Prot: ZN300_HUMAN, Q96RE9
    Tissue specificity: Expressed mostly in heart, skeletal muscle and brain. Isoform 1 and isoform 2 are highly expressed
    in testis

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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for ZNF300 gene from 1/8 species (see all 8)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves --
    --
    (see all 8)
    --
    41(a)
    34(a)
    (see all 8)
    many ↔ many
    many ↔ many
    (see all 8)
    Un(41687425-41690654)
    Un(16877163-16880802)


    ENSEMBL Gene Tree for ZNF300 (if available)
    TreeFam Gene Tree for ZNF300 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for ZNF300 gene
    ZNF1752  ZNF6742  LOC1002875152  ZNF6142  ZNF4322  ZNF6132  ZNF262  ZNF3502  
    ZNF812  ZNF6492  ZNF6052  ZNF5772  ZNF842  ZNF6302  ZFP372  ZNF4842  
    ENSG000002296312  ZNF1822  ZNF412  ZNF2682  LOC1009964642  ZNF6152  
    18/541 SIMAP similar genes for ZNF300 using alignment to 4 protein entries:     ZN300_HUMAN (see all proteins) (see all similar genes):
    ZNF66    ZNF78L1    MRPS17    ZNF24    kr-znf3    MGC12518
    POM121C    ZFS-5    ZNF391    ZNF436    DKFZp666M039    HZF36
    ZNF146    ZNF721    ZNF331    ZFP90    ZFP41    ZNF232

    ZNF300 for paralogs           About GeneDecksing


    4 Pseudogenes.org Pseudogenes for ZNF300
    PGOHUM00000234386 PGOHUM00000234444 PGOHUM00000235425 PGOHUM00000232570


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/245 NCBI SNPs in ZNF300 are shown (see all 245    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 5 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1157318081,2
    Cother155140539(+) TGAAAC/TCCACA 5 D G mis1 ut510--------
    rs766570701,2
    F,--145419374(+) TAACAT/CTGAGT 3 -- ds50011Minor allele frequency- C:0.02WA 118
    rs798991931,2
    F,--145419972(+) GCAGAG/ATGTCC 3 -- ut311Minor allele frequency- A:0.02WA 118
    rs1154690511,2
    C,F,--145420479(+) TTCCAC/TGTCTT 3 -- ut311Minor allele frequency- T:0.03WA 118
    rs791428781,2
    C,--145421822(+) TGTGAC/TTTCTT 6 K syn10--------
    rs44691891,2
    H--145422505(+) CAAAAA/GAAGGA 3 -- int1 trp34Minor allele frequency- G:0.00NS EA 418
    rs748644311,2
    --145423063(+) GCATTT/CATGTC 3 -- int11Minor allele frequency- C:0.01WA 118
    rs728078091,2
    --145423254(+) TTGCCG/TTCTAT 3 -- int10--------
    rs567376611,2
    F,--145423368(+) ATTACT/CTGTTG 3 -- int11Minor allele frequency- C:0.05WA 118
    rs117492181,2
    H--145424221(+) TGGGAA/GGAAGA 3 -- int1 trp30--------

    HapMap Linkage Disequilibrium report for ZNF300 (150273954 - 150284545 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 2 variations for ZNF300
         2 CNVs: 3580 32752

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing ZNF300
    DNA2.0 Custom Variant and Variant Library Synthesis for ZNF300

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    ZNF300 for disorders           About GeneDecksing

    OMIM gene information: 612429    OMIM disorders: --

    1 disease for ZNF300:    About MalaCards
    crohn's disease

    Human Genome Epidemiology (HuGE) Navigator: ZNF300 (2 documents)

    Export disorders for ZNF300 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for ZNF300 gene, integrated from 9 sources (see all 17):
    (articles sorted by number of sources associating them with ZNF300)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Identification and functional analysis of a novel human KRAB/C2H2 zinc finger gene ZNF300. (PubMed id 14746915)1, 2, 3 Gou D.-M.... Li W.-X. (2004)
    2. Molecular cloning and characterization of a novel splice variant of human ZNF300 gene, which expressed highly in testis. (PubMed id 17541838)1, 2, 9 Cao Y....Wu M. (2007)
    3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    5. Methods for quantification of in vivo changes in prote in ubiquitination following proteasome and deubiquitinase inhibition. (PubMed id 22505724)1 Udeshi N.D....Carr S.A. (2012)
    6. Overexpression of the human ZNF300 gene enhances growt h and metastasis of cancer cells through activating NF-kB pathway. (PubMed id 21777376)1 Wang T....Li W.X. (2012)
    7. Genome-wide searching of rare genetic variants in WTC CC data. (PubMed id 20549515)1 Feng T. and Zhu X. (2010)
    8. PU.1 can regulate the ZNF300 promoter in APL-derived promyelocytes HL-60. (PubMed id 20471086)1 Xu J.H....Li W.X. (2010)
    9. ZNF300, a recently identified human transcription fac tor, activates the human IL-2RI^ promoter through the overlapping ZNF300/EGR1 bi nding site. (PubMed id 20585888)1 Xue L....Li W. (2010)
    10. Identification of the DNA binding element of the human ZNF300 protein. (PubMed id 18350257)1 Qiu H....Li W. (2008)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 91975 HGNC: 13091 AceView: ZNF300 Ensembl:ENSG00000145908 euGenes: HUgn91975
    ECgene: ZNF300 H-InvDB: ZNF300

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
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    About This Section
    NameDescription
    PharmGKB entry for ZNF300 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
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    Patent Information for ZNF300 gene:
    Search GeneIP for patents involving ZNF300

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