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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

ZNF280D Gene

protein-coding   GIFtS: 47
GCID: GC15M056922

zinc finger protein 280D

(Previous name: suppressor of hairy wing homolog 4 (Drosophila) )
(Previous symbol: SUHW4)
 Explore 2 diseases affiliated with
ZNF280D via our new
 Human Malady Compendium 
Biological research products
for ZNF280D
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Zinc Finger Protein 280D1 2     Zinc Finger Protein 6342 3
SUHW41 2 3     FLJ200861
ZNF6341 2 3     Suppressor Of Hairy Wing Homolog 4 (Drosophila)1
Suppressor Of Hairy Wing Homolog 42 3     KIAA15843

External Ids:    HGNC: 259531   Entrez Gene: 548162   Ensembl: ENSG000001378717   UniProtKB: Q6N0433   

Export aliases for ZNF280D gene to outside databases

Previous GC identifers: GC15M054710 GC15M033745


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: Z280D_HUMAN, Q6N043
Function: May function as a transcription factor

Gene Wiki entry for ZNF280D (SUHW4)


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000015.9  NC_018926.1  NT_010194.17  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the ZNF280D gene promoter:
         Egr-3   Bach1   POU3F1   Egr-1   Egr-4   YY1   E2F   E2F-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidZNF280D promoter sequence
   Search SABiosciences Chromatin IP Primers for ZNF280D

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat ZNF280D


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 15q21.3   Ensembl cytogenetic band:  15q21.3   HGNC cytogenetic band: 15q21.2

ZNF280D Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ZNF280D gene location

GeneLoc information about chromosome 15         GeneLoc Exon Structure

GeneLoc location for GC15M056922:  view genomic region     (about GC identifiers)

Start:
56,922,374 bp from pter      End:
57,210,769 bp from pter
Size:
288,396 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: Z280D_HUMAN, Q6N043 (See protein sequence)
Recommended Name: Zinc finger protein 280D  
Size: 979 amino acids; 109285 Da
Subcellular location: Nucleus (Potential)
Sequence caution: Sequence=BAB13410.1; Type=Erroneous initiation; Sequence=CAE46003.1; Type=Erroneous initiation;
Secondary accessions: A1L495 B2RMT6 Q6MZM6 Q6N085 Q9H0U5 Q9HCI8 Q9NXS0
Alternative splicing: 5 isoforms:  Q6N043-1   Q6N043-2   Q6N043-3   Q6N043-4   Q6N043-5   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for ZNF280D: NX_Q6N043

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q6N043

  • ZNF280D Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (3 alternative transcripts): 
    NP_001002843.1  NP_001002844.1  NP_060131.2  

    ENSEMBL proteins: 
     ENSP00000454173   ENSP00000454111   ENSP00000267807   ENSP00000453636   ENSP00000453045  
     ENSP00000453124   ENSP00000453706   ENSP00000453912   ENSP00000379545  

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    Uscn Proteins for ZNF280D

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005622intracellular ----
    GO:0005634nucleus IEA--


    ZNF280D for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    ZNF280D for domains           About GeneDecksing

    3 InterPro domains/families:
     IPR015880 Znf_C2H2-like
     IPR025243 DUF4195
     IPR007087 Znf_C2H2

    Graphical View of Domain Structure for InterPro Entry Q6N043

    ProtoNet protein and cluster: Q6N043

    UniProtKB/Swiss-Prot: Z280D_HUMAN, Q6N043
    Similarity: Contains 5 C2H2-type zinc fingers


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: Z280D_HUMAN, Q6N043
    Function: May function as a transcription factor

    miRNA
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    hsa-miR-323-3p hsa-miR-181c hsa-miR-373* hsa-miR-9 hsa-miR-3658 hsa-miR-3613-3p hsa-miR-548x hsa-miR-613
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    Inhib. RNA
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    In Situ Assay
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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ZNF280D

    Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003677DNA binding IEA--
    GO:0008270zinc ion binding IEA--


    ZNF280D for ontologies           About GeneDecksing



    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for ZNF280D

    STRING Interaction Network Preview (showing 2 interactants - click image to see more details)

    2 Interacting proteins for ZNF280D (ENSP000002678074) via UniProtKB, MINT, STRING, and/or I2D
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CBX5ENSP000002098754STRING: ENSP00000209875
    CBX3ENSP000003366874STRING: ENSP00000336687
    About this table

    Gene Ontology (GO): 2 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006351transcription, DNA-dependent IEA--
    GO:0006355regulation of transcription, DNA-dependent IEA--


    ZNF280D for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for ZNF280D
    Search CenterWatch for drugs/clinical trials and news about ZNF280D / Z280D 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for ZNF280D gene (3 alternative transcripts): 
    NM_001002843.1  NM_001002844.1  NM_017661.2  

    Unigene Cluster for ZNF280D:

    Zinc finger protein 280D
    Hs.511477  [show with all ESTs]
    Unigene Representative Sequence: BC040532
    15 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000558067(uc002adt.3 uc010bfp.3) ENST00000559237(uc002adu.3 uc002adv.3 uc010bfq.3)
    ENST00000267807 ENST00000559446 ENST00000559182 ENST00000560388 ENST00000560002
    ENST00000559000(uc002adw.1) ENST00000558002(uc010bfr.1) ENST00000560121
    ENST00000561126 ENST00000558320(uc002adx.3 uc002ady.3) ENST00000559352
    ENST00000560298 ENST00000396245

    miRNA
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    8/30 QIAGEN miScript miRNA Assays for microRNAs that regulate ZNF280D (see all 30):
    hsa-miR-323-3p hsa-miR-181c hsa-miR-373* hsa-miR-9 hsa-miR-3658 hsa-miR-3613-3p hsa-miR-548x hsa-miR-613
    SwitchGear 3'UTR luciferase reporter plasmidZNF280D 3' UTR sequence
    Inhib. RNA
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    Browse for Gene Knock-down Tools from EMD Millipore
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    Additional cDNA sequence: 

    AB046804.1 AK000093.1 AK025073.1 AK289739.1 AK295159.1 AK310882.1 AL136634.1 BC015382.2 
    BC040532.1 BC053649.1 BC064359.1 BC130451.1 BC136412.1 BX640637.1 BX640707.1 BX641000.1 

    17 DOTS entries:

    DT.443432  DT.100686700  DT.75100354  DT.100021896  DT.97818903  DT.121029841  DT.100746115  DT.75115789 
    DT.40313330  DT.99974343  DT.101975270  DT.95160464  DT.100746114  DT.121029828  DT.121184949  DT.92425216 
    DT.99979036 

    24/165 AceView cDNA sequences (see all 165):

    AI399855 Z38469 BE672903 BM795112 BU689457 AI669753 AB046804 BE503719 
    NM_017661 F10420 BG035432 AI537169 NM_001002845 AA829850 AW271416 AA031977 
    AA927823 BU688176 BF436870 Z42224 BI915927 BQ549391 BU069398 AI613078 

    GeneLoc Exon Structure

    5/7 Alternative Splicing Database (ASD) splice patterns (SP) for ZNF280D (see all 7)    About this scheme

    ExUns: 1a · 1b · 1c ^ 2 ^ 3a · 3b ^ 4 ^ 5 ^ 6a · 6b · 6c ^ 7 ^ 8 ^ 9 ^ 10a · 10b ^ 11 ^ 12 ^ 13 ^ 14 ^ 15a · 15b ^ 16 ^ 17 ^ 18 ^ 19 ^
    SP1:                                                        -     -                                                                                   -         
    SP2:                          -     -                       -     -                                                                                   -         
    SP3:                          -                             -     -                                                                 -                 -         
    SP4:                                                                                                                                                            
    SP5:                          -                                                                                                                                 

    ExUns: 20a · 20b ^ 21 ^ 22a · 22b ^ 23a · 23b
    SP1:        -           -                     
    SP2:        -           -                     
    SP3:                                          
    SP4:        -           -                     
    SP5:                                          


    ECgene alternative splicing isoforms for ZNF280D

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    ZNF280D expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: TTTAAGTGAC

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image

    ZNF280D expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    Stem Cell Differentiation: 2 LifeMap Cells 
    NameCategory
    PureStem™ progenitor E44 (Embryonic Progenitor Cell)
    PureStem™ progenitor F15 (Embryonic Progenitor Cell)
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See ZNF280D Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for ZNF280D

    SOURCE GeneReport for Unigene cluster: Hs.511477
        SABiosciences Custom PCR Arrays for ZNF280D
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ZNF280D

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for ZNF280D gene from 6/17 species (see all 17)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Zfp280d1 , 5 zinc finger protein 280D1, 5 84.05(n)1
    77.53(a)1
      9 (40.08 cM)5
    2354691  NM_146224.41  NP_666336.31 
     722748995 
    chicken
    (Gallus gallus)
    Aves ZNF280D1 zinc finger protein 280D 71.15(n)
    64.83(a)
      415593  XM_413961.3  XP_413961.3 
    lizard
    (Anolis carolinensis)
    Reptilia --
    --
    47(a)
    1 → many
    GL343573.1(438826-450503)
    zebrafish
    (Danio rerio)
    Actinopterygii znf280d1 zinc finger protein 280D 52.23(n)
    46.74(a)
      556610  XM_679451.3  XP_684543.3 
    fruit fly
    (Drosophila melanogaster)
    Insecta row6
    relative of woc
    10(a)
    1 → many
    2R(11094032-11101137)
    worm
    (Caenorhabditis elegans)
    Secernentea F37D6.26
    Protein F37D6.2, isoform a
    17(a)
    1 → many
    I(10475513-10478120)


    ENSEMBL Gene Tree for ZNF280D (if available)
    TreeFam Gene Tree for ZNF280D (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for ZNF280D gene
    ZNF280A2  POGZ2  ZNF280B2  ZNF280C2  
    4 SIMAP similar genes for ZNF280D using alignment to 5 protein entries:     Z280D_HUMAN (see all proteins):
    ZNF280C    ZNF280B    ZNF280A    POGZ

    ZNF280D for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/1635 NCBI SNPs in ZNF280D are shown (see all 1635    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 15 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs113016521,2
    C,--33771262(+) ATCAG-/CCCCAC 1 -- int12Minor allele frequency- C:0.00NA CSA 4
    rs1427709721,2
    --56921903(+) ACAGGC/TGTTTT 2 -- int10--------
    rs118573881,2
    C,F,A,H,--56921934(+) GGTTTA/CTATTA 2 -- int114Minor allele frequency- C:0.45NA WA EA 680
    rs763454661,2
    F,--56921945(+) TTCCAA/GAAATC 2 -- int11Minor allele frequency- G:0.03WA 118
    rs1832838451,2
    --56922079(+) AATAAC/TTCATA 2 -- int10--------
    rs1131397431,2
    --56922155(+) ATTTCC/TCTTGA 2 -- int12Minor allele frequency- T:0.33CSA 3
    rs797669351,2
    F,--56922159(+) CTCTTG/AAAGTA 2 -- int11Minor allele frequency- A:0.03WA 118
    rs1929727761,2
    --56922180(+) TAAAGC/TAAATC 2 -- int10--------
    rs1510411491,2
    --56922357(+) AGCAGA/TGATTG 2 -- int10--------
    rs1424185671,2
    --56922360(+) AGTGAC/TTGGAA 2 -- int10--------

    HapMap Linkage Disequilibrium report for ZNF280D (56922374 - 57172374 bp, first 250kb of ZNF280D)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for ZNF280D: --

    SABiosciences Cancer Mutation PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    ZNF280D for disorders           About GeneDecksing

    2 diseases for ZNF280D:    About MalaCards
    apraxia    dyslexia

    4 diseases from the University of Copenhagen DISEASES database for ZNF280D:
    Articulation disorder     Dyslexia     Apraxia     DOID:4019

    Export disorders for ZNF280D gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for ZNF280D gene, integrated from 9 sources (see all 20):
    (articles sorted by number of sources associating them with ZNF280D)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. (PubMed id 10997877)1, 2, 3 Nagase T.... Ohara O. (2000)
    2. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    3. Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs. (PubMed id 11230166)1, 2 Wiemann S.... Poustka A. (2001)
    4. The proteomic analysis of endogenous FAT10 substrates identifies p62/SQSTM1 as a substrate of FAT10ylation. (PubMed id 22797925)1 Aichem A....Groettrup M. (2012)
    5. Charting the landscape of tandem BRCT domain-mediated protein interactions. (PubMed id 22990118)1 Woods N.T....Monteiro A.N. (2012)
    6. A cohort of balanced reciprocal translocations associ ated with dyslexia: identification of two putative candidate genes at DYX1. (PubMed id 20798984)1 Buonincontri R....Tommerup N. (2011)
    7. Analysis of the human HP1 interactome reveals novel bi nding partners. (PubMed id 21888893)1 Rosnoblet C....Angrand P.O. (2011)
    8. Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. (PubMed id 20068231)2 Olsen J.V....Mann M. (2010)
    9. Human POGZ modulates dissociation of HP1alpha from mi totic chromosome arms through Aurora B activation. (PubMed id 20562864)1 Nozawa R.S....Obuse C. (2010)
    10. Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis. (PubMed id 18220336)2 Cantin G.T....Yates J.R. III (2008)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 54816 HGNC: 25953 AceView: SUHW4 Ensembl:ENSG00000137871 euGenes: HUgn54816
    ECgene: ZNF280D H-InvDB: ZNF280D

    (According to HUGE)
    About This Section
    HUGE: KIAA1584

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for ZNF280D Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for ZNF280D gene:
    Search GeneIP for patents involving ZNF280D

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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    GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 26 Apr 2013

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