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ZNF256 Gene

protein-coding   GIFtS: 47
GCID: GC19M058452

Zinc Finger Protein 256

  Search for ZNF256
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Zinc Finger Protein 2561 2
Bone Marrow Zinc Finger 32 3
BMZF-32 3
BMZF32 3

External Ids:    HGNC: 130491   Entrez Gene: 101722   Ensembl: ENSG000001524547   OMIM: 6069565   UniProtKB: Q9Y2P73   

Export aliases for ZNF256 gene to outside databases

Previous GC identifers: GC19M059282 GC19M058905 GC19M063123 GC19M063143 GC19M063144 GC19M054763


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for ZNF256 Gene:
ZNF256 (zinc finger protein 256) is a protein-coding gene. GO annotations related to this gene include sequence-specific DNA binding transcription factor activity. An important paralog of this gene is ZNF549.

UniProtKB/Swiss-Prot: ZN256_HUMAN, Q9Y2P7
Function: Transcriptional repressor that plays a role in cell proliferation. Requires TRIM28 for its activity




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000019.9  NT_011109.17  NC_018930.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the ZNF256 gene promoter:
         GATA-3   AP-1   ATF-2   c-Jun   LCR-F1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidZNF256 promoter sequence
   Search Chromatin IP Primers for ZNF256

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat ZNF256


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 19q13.43   Ensembl cytogenetic band:  19q13.43   HGNC cytogenetic band: 19q13

ZNF256 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ZNF256 gene location

GeneLoc information about chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19M058452:  view genomic region     (about GC identifiers)

Start:
58,452,201 bp from pter      End:
58,459,077 bp from pter
Size:
6,877 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: ZN256_HUMAN, Q9Y2P7 (See protein sequence)
Recommended Name: Zinc finger protein 256  
Size: 627 amino acids; 71871 Da
Subunit: Interacts with TRIM28
Secondary accessions: B2RA92 Q53Y85 Q9BV71
Alternative splicing: 2 isoforms:  Q9Y2P7-1   Q9Y2P7-2   

Explore the universe of human proteins at neXtProt for ZNF256: NX_Q9Y2P7

Explore proteomics data for ZNF256 at MOPED

Post-translational modifications: 

  • Ubiquitination2 at Lys447
  • Modification sites at PhosphoSitePlus

  • See ZNF256 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_005764.2  
    ENSEMBL proteins: 
     ENSP00000282308   ENSP00000472858  
    Reactome Protein details: Q9Y2P7

    ZNF256 Human Recombinant Protein Products:

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    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    ZNF: Zinc fingers, C2H2-type

    4 InterPro protein domains:
     IPR001909 Krueppel-associated_box
     IPR015880 Znf_C2H2-like
     IPR013087 Znf_C2H2/integrase_DNA-bd
     IPR007087 Znf_C2H2

    Graphical View of Domain Structure for InterPro Entry Q9Y2P7

    ProtoNet protein and cluster: Q9Y2P7

    1 Blocks protein domain: IPB007086 C2H2-type zinc finger signature

    UniProtKB/Swiss-Prot: ZN256_HUMAN, Q9Y2P7
    Domain: The KRAB domain mediates interaction with TRIM28 and is required for transcriptional repressor activity
    Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family
    Similarity: Contains 15 C2H2-type zinc fingers
    Similarity: Contains 1 KRAB domain


    Find genes that share domains with ZNF256           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: ZN256_HUMAN, Q9Y2P7
    Function: Transcriptional repressor that plays a role in cell proliferation. Requires TRIM28 for its activity
    Induction: By GDNF/glial cell line-derived neurotrophic factor

         Gene Ontology (GO): 5 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003676nucleic acid binding ----
    GO:0003677DNA binding IEA--
    GO:0003700sequence-specific DNA binding transcription factor activity TAS10585455
    GO:0005515protein binding IPI18060868
    GO:0046872metal ion binding IEA--
         
    Find genes that share ontologies with ZNF256           About GenesLikeMe


    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for ZNF256
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    miRNA
    Products:
        
    miRTarBase miRNAs that target ZNF256:
    hsa-mir-744-5p (MIRT037511), hsa-let-7e-5p (MIRT051585)

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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ZNF256


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    ZN256_HUMAN, Q9Y2P7: Nucleus
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    cytosol2

    Gene Ontology (GO): 2 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005622intracellular ----
    GO:0005634nucleus IDA--

    Find genes that share ontologies with ZNF256           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for ZNF256 About    
    See pathways by source

    SuperPathContained pathways About
    1Gene Expression
    Generic Transcription Pathway0.40
    Gene Expression0.40


    Find genes that share SuperPaths with ZNF256           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 Reactome Pathway for ZNF256
        Generic Transcription Pathway


        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for ZNF256
    Interactions:

        GeneGlobe Interaction Network for ZNF256

    Gene Ontology (GO): 4 biological process terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006351transcription, DNA-templated IEA--
    GO:0006355regulation of transcription, DNA-templated ----
    GO:0007275multicellular organismal development TAS10585455
    GO:0045892negative regulation of transcription, DNA-templated IDA18060868

    Find genes that share ontologies with ZNF256           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for ZNF256 (ZN256)



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for ZNF256 gene: 
    NM_005773.2  

    Unigene Cluster for ZNF256:

    Zinc finger protein 256
    Hs.596242  [show with all ESTs]
    Unigene Representative Sequence: NM_005773
    2 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000282308(uc002qqu.3 uc010euj.3) ENST00000598928
    miRNA
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    Browse SwitchGear 3'UTR luciferase reporter plasmids
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat ZNF256
    Clone
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    GenScript: all cDNA clones in your preferred vector: ZNF256 (NM_005773)
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    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat ZNF256
    Primer
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    OriGene qPCR primer pairs and template standards for ZNF256
    OriGene qSTAR qPCR primer pairs in human, mouse for ZNF256
    Pre-validated RT2 qPCR Primer Assay in human, mouse / rat ZNF256
      QuantiTect SYBR Green Assays in human, mouse, rat ZNF256
      QuantiFast Probe-based Assays in human, mouse, rat ZNF256

    Additional mRNA sequence: 

    AF067165.1 AK314094.1 BC001438.1 BT006853.1 

    4 DOTS entries:

    DT.95152550  DT.95227492  DT.70103719  DT.75177852 

    Selected AceView cDNA sequences (see all 28):

    BM452054 BC001438 BF508722 BF510334 AI087275 NM_005773 BT006853 CA406341 
    BG287031 AW151772 BP335651 BG336545 BE265862 BG481284 CD656453 BX475520 
    AF067165 AU280306 AW054759 BV191696 AA306773 BM463060 AA507587 BI116995 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    ZNF256 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: AGAGAGTTCA
    ZNF256 Expression
    About this image

    ZNF256 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    ZNF256 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.596242
        Custom PCR Arrays for ZNF256
    Primer
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ZNF256

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of mammals.

    Orthologs for ZNF256 gene from Selected species (see all 3)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    dog
    (Canis familiaris)
    Mammalia LOC4914621 zinc finger protein 132-like 69.21(n)
    55.12(a)
      491462  XM_005616832.1  XP_005616889.1 


    ENSEMBL Gene Tree for ZNF256 (if available)
    TreeFam Gene Tree for ZNF256 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for ZNF256 gene
    ZNF5492  ENSG000002695332  ZNF4172  ENSG000002681332  ENSG000002681072  ZNF4162  ZNF172  ZNF5482  
    ZNF587B2  ZNF7732  ZIK12  ZNF1342  ZNF6712  ENSG000002681632  ZNF7722  ZNF5302  
    ZNF4192  ZNF5872  ZNF2112  ZNF7922  ZNF7492  ZNF5512  ENSG000002687502  ZNF7762  
    ZNF5472  ZNF5842  ENSG000002690262  ZNF3042  ZNF1322  ZNF5862  ZNF4182  ZNF5522  
    ZNF1542  
    Selected SIMAP similar genes for ZNF256 using alignment to 3 protein entries:     ZN256_HUMAN (see all proteins) (see all similar genes):
    DKFZp666C237    HZF36    ZNF232    ZNF436    DKFZp666M039    ZNF383
    HZF30    ZKSCAN1    ZNF146    ZNF485    ZNF852    ZNF883
    ZSCAN16    ZSCAN21    DKFZp686N14223    ZFP92    ZFS-1    ZNF3

    Find genes that share paralogs with ZNF256           About GenesLikeMe


    1 Pseudogenes.org Pseudogene for ZNF256
    PGOHUM00000241151


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for ZNF256 (see all 220)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 19 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1131985471,2
    C--58456282(+) ATTAA-/TTTTTT 1 -- int11Minor allele frequency- T:0.00CSA 2
    rs1835954671,2
    --58600910(+) GGGTAA/GTGGGT 1 -- int10--------
    rs1887068091,2
    --58601022(+) TAATAC/TATAAT 1 -- int10--------
    rs104061421,2
    C,F,H--58601096(+) tttgaG/Agtgat 1 -- int112Minor allele frequency- A:0.02NS NA WA 1722
    rs1170560351,2
    F--58601187(+) ATTTGT/CCCATT 1 -- int11Minor allele frequency- C:0.02EA 120
    rs1812166611,2
    --58601213(+) TTTTTA/TAAAAA 1 -- int10--------
    rs1448105561,2
    --58601331(+) GGTTAC/TTGAAA 1 -- ut310--------
    rs1999925571,2
    C--58601390(+) AGTCCG/TTAACA 1 -- ut310--------
    rs1385789981,2
    C--58601520(+) AATTCA/GCTGGT 2 R * stg10--------
    rs1494054011,2
    C--58601597(+) CGGTAT/CGAACT 2 /H /R mis11Minor allele frequency- C:0.00NA 4550

    HapMap Linkage Disequilibrium report for ZNF256 (58452201 - 58459077 bp)

    Structural Variations
          Database of Genomic Variants (DGV) variations for ZNF256: --
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing ZNF256
    DNA2.0 Custom Variant and Variant Library Synthesis for ZNF256

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 606956    OMIM disorders: --


    Find genes that share disorders with ZNF256           About GenesLikeMe


    Export disorders for ZNF256 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for ZNF256 gene integrated from 10 sources:
    (articles sorted by number of sources associating them with ZNF256)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. A novel GDNF-inducible gene, BMZF3, encodes a transcriptional repressor associated with KAP-1. (PubMed id 18060868)1, 2 Suzuki C.... Takahashi M. (Biochem. Biophys. Res. Commun. 2008)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    3. Molecular cloning of six novel Kruppel-like zinc finger genes from hematopoietic cells and identification of a novel transregulatory domain KRNB. (PubMed id 10585455)1, 2 Han Z.-G.... Chen Z. (J. Biol. Chem. 1999)
    4. A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (amp 2011)
    5. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)2 Ota T.... Sugano S. (Nat. Genet. 2004)
    6. The DNA sequence and biology of human chromosome 19. (PubMed id 15057824)2 Grimwood J.... Lucas S.M. (Nature 2004)
    7. Redifferentiation of dedifferentiated chondrocytes and chondrogenesis of human bone marrow stromal cells via chondrosphere formation with expression profiling by large-scale cDNA analysis. (PubMed id 12878157)1 Imabayashi H.... Umezawa A. (Exp. Cell Res. 2003)
    8. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 10172 HGNC: 13049 AceView: ZNF256 Ensembl:ENSG00000152454 euGenes: HUgn10172
    ECgene: ZNF256 H-InvDB: ZNF256

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for ZNF256 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for ZNF256 gene:
    Search GeneIP for patents involving ZNF256

    GeneCards and IP:
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    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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     Block miRNA regulation of human, mouse, rat ZNF256 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing ZNF256
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat ZNF256 Predesigned siRNA for gene silencing in human, mouse, rat ZNF256
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