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ZNF221 Gene

protein-coding   GIFtS: 48
GCID: GC19P044455

Zinc Finger Protein 221

  See related disease
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Zinc Finger Protein 2211 2

External Ids:    HGNC: 130141   Entrez Gene: 76382   Ensembl: ENSG000001599057   UniProtKB: Q9UK133   

Export aliases for ZNF221 gene to outside databases

Previous GC identifers: GC19P045100 GC19P044847 GC19P049131 GC19P049147 GC19P044271


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for ZNF221 Gene:
ZNF221 (zinc finger protein 221) is a protein-coding gene. Diseases associated with ZNF221 include azoospermia. An important paralog of this gene is ZNF229.

UniProtKB/Swiss-Prot: ZN221_HUMAN, Q9UK13
Function: May be involved in transcriptional regulation




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000019.10  NT_011109.17  NC_018930.2  
Regulatory elements:
   Search for regulatory transcription factor binding sites for ZNF221
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidZNF221 promoter sequence
   Search Chromatin IP Primers for ZNF221

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat ZNF221


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 19q13.2   Ensembl cytogenetic band:  19q13.31   HGNC cytogenetic band: 19q13.31

ZNF221 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ZNF221 gene location

GeneLoc information about chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19P044455:  view genomic region     (about GC identifiers)

Start:
44,455,375 bp from pter      End:
44,471,861 bp from pter
Size:
16,487 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: ZN221_HUMAN, Q9UK13 (See protein sequence)
Recommended Name: Zinc finger protein 221  
Size: 617 amino acids; 71170 Da
Secondary accessions: B2RAI6 Q2M2H2 Q9P1U8

Explore the universe of human proteins at neXtProt for ZNF221: NX_Q9UK13

Post-translational modifications: 

  • Ubiquitination2 at Lys280
  • Modification sites at PhosphoSitePlus
  • REFSEQ proteins: NP_037491.2  
    ENSEMBL proteins: 
     ENSP00000464885   ENSP00000467367   ENSP00000251269   ENSP00000467446  
    Reactome Protein details: Q9UK13

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    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    ZNF: Zinc fingers, C2H2-type

    4 InterPro protein domains:
     IPR001909 Krueppel-associated_box
     IPR015880 Znf_C2H2-like
     IPR013087 Znf_C2H2/integrase_DNA-bd
     IPR007087 Znf_C2H2

    Graphical View of Domain Structure for InterPro Entry Q9UK13

    ProtoNet protein and cluster: Q9UK13

    1 Blocks protein domain: IPB001909 KRAB box

    UniProtKB/Swiss-Prot: ZN221_HUMAN, Q9UK13
    Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family
    Similarity: Contains 15 C2H2-type zinc fingers
    Similarity: Contains 1 KRAB domain


    ZNF221 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: ZN221_HUMAN, Q9UK13
    Function: May be involved in transcriptional regulation

         Gene Ontology (GO): 3 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003676nucleic acid binding ----
    GO:0003677DNA binding IEA--
    GO:0046872metal ion binding IEA--
         
    ZNF221 for ontologies           About GeneDecksing


    Phenotypes:
         1 GenomeRNAi human phenotype for ZNF221:
     Wnt reporter downregulated 

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for ZNF221
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    miRTarBase miRNAs that target ZNF221:
    hsa-mir-335-5p (MIRT017933)

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    3 qRT-PCR Assays for microRNAs that regulate ZNF221:
    hsa-miR-188-3p hsa-miR-129-3p hsa-miR-129*
    SwitchGear 3'UTR luciferase reporter plasmidZNF221 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat ZNF221

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    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    ZN221_HUMAN, Q9UK13: Nucleus (Probable)
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus4
    cytosol2

    Gene Ontology (GO): 2 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005622intracellular ----
    GO:0005634nucleus IEA--

    ZNF221 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for ZNF221 About    
    See pathways by source

    SuperPathContained pathways About
    1Gene Expression
    Generic Transcription Pathway0.40
    Gene Expression0.40

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 Reactome Pathway for ZNF221
        Generic Transcription Pathway



    ZNF221 for pathways           About GeneDecksing

        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for ZNF221
    Interactions:

        Search GeneGlobe Interaction Network for ZNF221

    Gene Ontology (GO): 2 biological process terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006351transcription, DNA-templated IEA--
    GO:0006355regulation of transcription, DNA-templated IEA--

    ZNF221 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for ZNF221 (ZN221)



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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    REFSEQ mRNAs for ZNF221 gene: 
    NM_013359.2  

    Unigene Cluster for ZNF221:

    Zinc finger protein 221
    Hs.631598  [show with all ESTs]
    Unigene Representative Sequence: AF187987
    4 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000591168 ENST00000587682 ENST00000251269(uc002oxx.2 uc010ejb.1 uc010xws.1)
    ENST00000592350
    miRNA
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    3 qRT-PCR Assays for microRNAs that regulate ZNF221:
    hsa-miR-188-3p hsa-miR-129-3p hsa-miR-129*
    SwitchGear 3'UTR luciferase reporter plasmidZNF221 3' UTR sequence
    Inhib. RNA
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    OriGene RNAi products in human, mouse, rat for ZNF221
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    Primer
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    Pre-validated RT2 qPCR Primer Assay in human, mouse / rat ZNF221
      QuantiTect SYBR Green Assays in human, mouse, rat ZNF221
      QuantiFast Probe-based Assays in human, mouse, rat ZNF221

    Additional mRNA sequence: 

    AF187987.1 AK314207.1 BC111981.1 BC113426.1 BC143659.1 

    2 DOTS entries:

    DT.91669273  DT.445548 

    7 AceView cDNA sequences:

    NM_013359 BP360148 BM856938 BP337374 CN484052 AF187987 H65275 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    ZNF221 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TCTTTTGAAA
    ZNF221 Expression
    About this image

    ZNF221 Protein expression data from MOPED1, PaxDb2 and MaxQB3 --

    SOURCE GeneReport for Unigene cluster: Hs.631598
        Custom PCR Arrays for ZNF221
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for ZNF221

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of chordates.

    Orthologs for ZNF221 gene from 2 species (see representative species )    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chimpanzee
    (Pan troglodytes)
    Mammalia ZNF2211 zinc finger protein 221 98.38(n)
    96.76(a)
      456104  XM_003316419.1  XP_003316467.1 
    zebrafish
    (Danio rerio)
    Actinopterygii AF157109.12   -- 75.62(n)   30167  AF157109.1 


    ENSEMBL Gene Tree for ZNF221 (if available)
    TreeFam Gene Tree for ZNF221 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for ZNF221 gene
    ZNF2292  ZNF2232  ZNF2302  ZNF1552  ZNF2272  ZNF2242  ZNF2342  ZNF2252  
    ZNF2262  ZNF2142  ZNF2222  ZNF2842  ENSG000002671732  ZNF452  ZNF2352  ZNF2852  
    ENSG000002670222  ZNF1122  ZNF2332  
    Selected SIMAP similar genes for ZNF221 using alignment to 2 protein entries:     ZN221_HUMAN (see all proteins) (see all similar genes):
    ZNF224    ZNF155    ZNF225    ZNF625-ZNF20    ZNF230    ZNF223
    ZNF284    ZNF78L1    ZNF234    LOC101928664    ZNF55    ZNF222
    ZNF169    ZNF596    ZNF226    ZNF818P    ZNF83    ZNF624

    ZNF221 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for ZNF221 (see all 502)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 19 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1872009031,2
    --44453421(+) CAGGGA/GGGCCC 1 -- us2k10--------
    rs1910689031,2
    --44453710(+) TGATAC/GTATTT 1 -- us2k10--------
    rs780540691,2
    C,F--44453742(+) TTTCTA/GTATCA 1 -- us2k12Minor allele frequency- G:0.05WA 120
    rs1417863191,2
    C--44453978(+) CTGTT-/AAAAAA 1 -- us2k10--------
    rs1821419971,2
    --44454045(+) AAAAAA/CCCAGA 1 -- us2k10--------
    rs1470328951,2
    C--44454060(+) TTGAGA/GCTATC 1 -- us2k10--------
    rs796297681,2
    C,F--44454106(+) CTACAG/CAGCTA 1 -- us2k11Minor allele frequency- C:0.50WA 2
    rs1438999581,2
    --44454108(+) ACAGAA/GCTATA 1 -- us2k10--------
    rs1142330731,2
    C,F--44454201(+) ACCTAC/TGGATA 1 -- us2k11Minor allele frequency- T:0.03WA 118
    rs1853934841,2
    --44454247(+) GAAAAC/GACAGT 1 -- us2k10--------

    HapMap Linkage Disequilibrium report for ZNF221 (44455375 - 44471861 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 2 variations for ZNF221:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv912109CNV Loss21882294
    nsv469650CNV Gain16826518

    Site Specific Mutation Identification with PCR Assays
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    DNA2.0 Custom Variant and Variant Library Synthesis for ZNF221

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    1 disease for ZNF221:    
    About MalaCards
    azoospermia


    ZNF221 for disorders           About GeneDecksing


    Export disorders for ZNF221 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for ZNF221 gene integrated from 10 sources:
    (articles sorted by number of sources associating them with ZNF221)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. The DNA sequence and biology of human chromosome 19. (PubMed id 15057824)1, 2 Grimwood J.... Lucas S.M. (Nature 2004)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    3. Differential expansion of zinc-finger transcription factor loci in homologous human and mouse gene clusters. (PubMed id 12743021)1, 2 Shannon M.... Stubbs L. (Genome Res. 2003)
    4. Sumoylation of heterogeneous nuclear ribonucleoproteins, zinc finger proteins, and nuclear pore complex proteins: a proteomic analysis. (PubMed id 15161980)1 Li T.... Chock P.B. (Proc. Natl. Acad. Sci. U.S.A. 2004)
    5. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)2 Ota T.... Sugano S. (Nat. Genet. 2004)
    6. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)
    7. Coding region intron/exon organization, alternative splicing and X- chromosome inactivation of the KRAB/FPB-domain-containing human zinc finger gene ZNF41. (PubMed id 10449920)1 Rosati M.... Grimaldi G. (Cytogenet. Cell Genet. 1999)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 7638 HGNC: 13014 AceView: ZNF221 Ensembl:ENSG00000159905 euGenes: HUgn7638
    ECgene: ZNF221 H-InvDB: ZNF221

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for ZNF221 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for ZNF221 gene:
    Search GeneIP for patents involving ZNF221

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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