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ZNF212 Gene

protein-coding   GIFtS: 52
GCID: GC07P148936

Zinc Finger Protein 212

  Search for ZNF212
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Zinc Finger Protein 2121 2
ZNFC1502 3 5
Zinc Finger Protein C2H2-1502 3
ZNF1822 5
C2H2-1502

External Ids:    HGNC: 130041   Entrez Gene: 79882   Ensembl: ENSG000001702607   OMIM: 6023865   UniProtKB: Q9UDV63   

Export aliases for ZNF212 gene to outside databases

Previous GC identifers: GC07P147194 GC07P148251 GC07P148328 GC07P148374 GC07P148567 GC07P143014


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for ZNF212 Gene:
This gene belongs to the C2H2-type zinc finger gene family. The zinc finger proteins are involved in gene
regulation and development, and are quite conserved throughout evolution. Like this gene product, a third of the
zinc finger proteins containing C2H2 fingers also contain the KRAB domain, which has been found to be involved in
protein-protein interactions. (provided by RefSeq, Jul 2008)

GeneCards Summary for ZNF212 Gene:
ZNF212 (zinc finger protein 212) is a protein-coding gene. GO annotations related to this gene include identical protein binding. An important paralog of this gene is ZNF282.

UniProtKB/Swiss-Prot: ZN212_HUMAN, Q9UDV6
Function: May be involved in transcriptional regulation




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000007.14  NT_007933.16  NC_018918.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the ZNF212 gene promoter:
         AP-2alpha isoform 3   Lmo2   Nkx2-5   AP-2alpha isoform 2   STAT3   AP-2alpha isoform 4   AP-2alpha   AP-2alphaA   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidZNF212 promoter sequence
   Search Chromatin IP Primers for ZNF212

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat ZNF212


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 7q36.1   Ensembl cytogenetic band:  7q36.1   HGNC cytogenetic band: 7q36.1

ZNF212 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
ZNF212 gene location

GeneLoc information about chromosome 7         GeneLoc Exon Structure

GeneLoc location for GC07P148936:  view genomic region     (about GC identifiers)

Start:
148,936,742 bp from pter      End:
148,952,700 bp from pter
Size:
15,959 bases      Orientation:
plus strand

1 alternative location:
Chr7+,CRA_TCAG 148,274,750-148,290,707     

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
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UniProtKB/Swiss-Prot: ZN212_HUMAN, Q9UDV6 (See protein sequence)
Recommended Name: Zinc finger protein 212  
Size: 495 amino acids; 55447 Da
Sequence caution: Sequence=AAC51296.1; Type=Erroneous initiation; Sequence=AAD45826.2; Type=Erroneous initiation;
Secondary accessions: B2RCF4 Q13396 Q8N664

Explore the universe of human proteins at neXtProt for ZNF212: NX_Q9UDV6

Explore proteomics data for ZNF212 at MOPED

Post-translational modifications: 

  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See ZNF212 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_036388.2  
    ENSEMBL proteins: 
     ENSP00000338572   ENSP00000418281   ENSP00000418167   ENSP00000419419   ENSP00000419261  
    Reactome Protein details: Q9UDV6

    ZNF212 Human Recombinant Protein Products:

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    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    ZNF: Zinc fingers, C2H2-type

    5 InterPro protein domains:
     IPR001909 Krueppel-associated_box
     IPR015880 Znf_C2H2-like
     IPR013087 Znf_C2H2/integrase_DNA-bd
     IPR022137 DUF3669_Znf
     IPR007087 Znf_C2H2

    Graphical View of Domain Structure for InterPro Entry Q9UDV6

    ProtoNet protein and cluster: Q9UDV6

    1 Blocks protein domain: IPB001909 KRAB box

    UniProtKB/Swiss-Prot: ZN212_HUMAN, Q9UDV6
    Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family
    Similarity: Contains 4 C2H2-type zinc fingers
    Similarity: Contains 1 KRAB domain


    ZNF212 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: ZN212_HUMAN, Q9UDV6
    Function: May be involved in transcriptional regulation

         Genatlas biochemistry entry for ZNF212:
    zinc finger protein 122,KRAB subfamily (C2H2 Kruppel-like)

         Gene Ontology (GO): Selected molecular function terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003676nucleic acid binding ----
    GO:0003677DNA binding IEA--
    GO:0005515protein binding IPI18255255
    GO:0008270zinc ion binding NAS7633419
    GO:0042802identical protein binding IPI18255255
         
    ZNF212 for ontologies           About GeneDecksing


    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for ZNF212
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    miRNA
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    hsa-mir-125b-5p (MIRT045981), hsa-mir-744-5p (MIRT037645)

    Block miRNA regulation of human, mouse, rat ZNF212 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate ZNF212 (see all 13):
    hsa-miR-4324 hsa-miR-3184 hsa-miR-185* hsa-miR-449c* hsa-miR-1258 hsa-miR-944 hsa-miR-3914 hsa-miR-7
    SwitchGear 3'UTR luciferase reporter plasmidZNF212 3' UTR sequence
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    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    ZN212_HUMAN, Q9UDV6: Nucleus (Potential)
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    cytosol2

    Gene Ontology (GO): 2 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005622intracellular ----
    GO:0005634nucleus IEA--

    ZNF212 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for ZNF212 About    
    See pathways by source

    SuperPathContained pathways About
    1Gene Expression
    Generic Transcription Pathway0.40
    Gene Expression0.40

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 Reactome Pathway for ZNF212
        Generic Transcription Pathway



    ZNF212 for pathways           About GeneDecksing

        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for ZNF212
    Interactions:

        Search GeneGlobe Interaction Network for ZNF212

    STRING Interaction Network Preview (showing 5 interactants - click image to see 19)

    Selected Interacting proteins for ZNF212 (Q9UDV61, 3 ENSP000003385724) via UniProtKB, MINT, STRING, and/or I2D (see all 27)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    GNL1P369153, ENSP000003658064I2D: score=1 STRING: ENSP00000365806
    ENSG00000206492P369153I2D: score=1 
    ENSG00000226882P369153I2D: score=1 
    ENSG00000228581P369153I2D: score=1 
    ENSG00000229470P369153I2D: score=1 
    About this table

    Gene Ontology (GO): 2 biological process terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006351transcription, DNA-templated IEA--
    GO:0006355regulation of transcription, DNA-templated NAS7633419

    ZNF212 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for ZNF212 (ZN212)



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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    REFSEQ mRNAs for ZNF212 gene: 
    NM_012256.3  

    Unigene Cluster for ZNF212:

    Zinc finger protein 212
    Hs.490510  [show with all ESTs]
    Unigene Representative Sequence: NM_012256
    5 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000335870(uc003wfp.3) ENST00000486371 ENST00000462724 ENST00000481584
    ENST00000488917
    miRNA
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    hsa-miR-4324 hsa-miR-3184 hsa-miR-185* hsa-miR-449c* hsa-miR-1258 hsa-miR-944 hsa-miR-3914 hsa-miR-7
    SwitchGear 3'UTR luciferase reporter plasmidZNF212 3' UTR sequence
    Inhib. RNA
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    Predesigned siRNA for gene silencing in human, mouse, rat ZNF212
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    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat ZNF212
      QuantiTect SYBR Green Assays in human, mouse, rat ZNF212
      QuantiFast Probe-based Assays in human, mouse, rat ZNF212

    Additional mRNA sequence: 

    AK074821.1 AK315086.1 BC022785.2 U38864.1 

    14 DOTS entries:

    DT.446324  DT.91950902  DT.121050997  DT.102834071  DT.91683890  DT.95096988  DT.95268179  DT.100804303 
    DT.121050913  DT.95268177  DT.97845530  DT.40240589  DT.95134411  DT.99935364 

    3 AceView cDNA sequences:

    BV186394 CB988192 CF593491 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for ZNF212 (see all 9)    About this scheme

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5a · 5b · 5c ^ 6a · 6b ^ 7a · 7b ^ 8a · 8b ^ 9a · 9b ^ 10a · 10b · 10c
    SP1:                                                                          -           -                           
    SP2:                                                                          -     -     -                           
    SP3:                                                                                      -                           
    SP4:        -     -           -                                                                                       
    SP5:        -           -     -                                                                                       


    ECgene alternative splicing isoforms for ZNF212

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    ZNF212 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TCTTCCCACG
    ZNF212 Expression
    About this image

    ZNF212 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    ZNF212 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.490510
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    In Situ
    Assay Products:
     

     
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of chordates.

    Orthologs for ZNF212 gene from Selected species (see all 9)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Zfp2121 , 5 Zinc finger protein 2121, 5 82.21(n)1
    80.04(a)1
      6 (22.94 cM)5
    2327841  NM_145576.21  NP_663551.21 
     479204765 
    chicken
    (Gallus gallus)
    Aves --
    Uncharacterized protein
    24(a)
    many ↔ many
    2(482598-491135)
    lizard
    (Anolis carolinensis)
    Reptilia ZNF2126
    zinc finger protein 212
    29(a)
    1 ↔ 1
    AAWZ02037767(3896-11046)


    ENSEMBL Gene Tree for ZNF212 (if available)
    TreeFam Gene Tree for ZNF212 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for ZNF212 gene
    ZNF2822  ZNF7772  ZNF7462  ZNF3982  ZNF7832  
    Selected SIMAP similar genes for ZNF212 using alignment to 4 protein entries:     ZN212_HUMAN (see all proteins) (see all similar genes):
    ZNF767    EML2    CLUL1    DCTN5    FAM175A    NF2
    AURA2    BTBD2    CHID1    FAM3A    RNF217    RUFY1
    TMEM14B    ZNF564    ZNF585B    ZNF74    LYRM4    TNFRSF17

    ZNF212 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for ZNF212 (see all 466)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 7 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1834594551,2
    C--148934745(+) GTATAA/CATGTG 1 -- us2k10--------
    rs125343091,2
    C,F,H--148934783(+) CAGAAG/ACTACC 1 -- us2k1 tfbs35Minor allele frequency- A:0.02NS EA NA 536
    rs1878039471,2
    C--148934788(+) GCTACC/GTGGCA 1 -- us2k10--------
    rs1487695831,2
    --148934881(+) TCAGCA/CTCCTA 1 -- us2k10--------
    rs1929685201,2
    --148934884(+) GCATCC/TTATGT 1 -- us2k10--------
    rs125402551,2
    H--148935039(+) aacctC/Tttgag 1 -- us2k10--------
    rs1424180811,2
    --148935065(+) GTGTGA/CACCAC 1 -- us2k10--------
    rs1380782291,2
    C--148935083(+) GGCTC-/ATTTTA
            
    ATTTT
    1 -- us2k10--------
    rs1852452151,2
    --148935104(+) TAATTC/TTGTAG 1 -- us2k10--------
    rs1513218111,2
    C--148935116(+) GACAGG/TGTCTG 1 -- us2k10--------

    HapMap Linkage Disequilibrium report for ZNF212 (148936742 - 148952700 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 3 variations for ZNF212:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv465196CNV Loss19166990
    nsv889402CNV Loss21882294
    nsv819456CNV Gain19587683

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing ZNF212
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 602386    OMIM disorders: --


    ZNF212 for disorders           About GeneDecksing

    Genetic Association Database (GAD): ZNF212

    Export disorders for ZNF212 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for ZNF212 gene, integrated from 10 sources (see all 11):
    (articles sorted by number of sources associating them with ZNF212)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Molecular cloning and mapping of a novel human KRAB domain-containing C2H2-type zinc finger to chromosome 7q36.1. (PubMed id 9169157)1, 2, 3 Becker K.G.... Drew P.D. (Genomics 1997)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    3. The DNA sequence of human chromosome 7. (PubMed id 12853948)1, 2 Hillier L.W.... Wilson R.K. (Nature 2003)
    4. Rapid isolation and characterization of 118 novel C2H2-type zinc finger cDNAs expressed in human brain. (PubMed id 7633419)1, 2 Becker K.G....Drew P.D. (Hum. Mol. Genet. 1995)
    5. A Y2H-seq approach defines the human protein methyltransferase interactome. (PubMed id 23455924)1 Weimann M.... Stelzl U. (Nat. Methods 2013)
    6. An atlas of combinatorial transcriptional regulation in mouse and man. (PubMed id 20211142)1 Ravasi T....Hayashizaki Y. (Cell 2010)
    7. A protein-protein interaction network of transcription factors acting during liver cell proliferation. (PubMed id 18255255)1 Gao J....Yu Y. (Genomics 2008)
    8. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)2 Ota T.... Sugano S. (Nat. Genet. 2004)
    9. Human chromosome 7: DNA sequence and biology. (PubMed id 12690205)1 Scherer S.W.... Tsui L.-C. (Science 2003)
    10. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)

    (in PubMed, OMIM, and NCBI Bookshelf)
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      Query String
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 7988 HGNC: 13004 AceView: ZNF212.1 Ensembl:ENSG00000170260 euGenes: HUgn7988
    ECgene: ZNF212 H-InvDB: ZNF212

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

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    NameDescription
    PharmGKB entry for ZNF212 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for ZNF212 gene:
    Search GeneIP for patents involving ZNF212

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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