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ZC3H8 Gene

protein-coding GIFtS: 46

GC02M112690

zinc finger CCCH-type containing 8
(Previous name: zinc finger CCCH-type domain containing 8 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: ZC3HDC8)

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
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Aliases
Fliz1 ^¹, ²
ZC3HDC8 ^², ³

Descriptions
zinc finger CCCH type domain containing 8 ^²
zinc finger CCCH-type containing 8 ^²
zinc finger CCCH-type domain containing 8 ^¹, ²

External Ids
HGNC: 30941¹
Entrez Gene: 84524²
UniProtKB: Q8N5P1³
Ensembl: ENSG00000144161⁷

Search outside databases for aliases for ZC3H8 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the ZC3H8 gene

Entrez Gene cytogenetic band: 2q13 Ensembl cytogenetic band: 2q13 HGNC cytogenetic band: 2q13

ZC3H8 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2 GeneLoc Exon Structure

GeneLoc location for GC02M112690: (about GC identifiers)

Start:	112,689,725 bp from pter
End:	112,729,159 bp from pter
Size:	39,435 bases
Orientation:	minus strand

RefSeq DNA sequence:

NC_000002.10 NT_022135.15

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: ZC3H8_HUMAN, Q8N5P1 (See protein sequence)

Recommended Name: Zinc finger CCCH domain-containing protein 8

Size: 291 amino acids; 33576 Da

Subcellular location: Nucleus (By similarity)

Sequence caution: Sequence=AAH32001.1; Type=Frameshift; Positions=291;

Secondary accessions: Q9BZ75

Post-translational modifications:

Phosphorylated upon DNA damage, probably by ATM or ATR¹

View phosphorylation sites using PhosphoSite²

REFSEQ proteins: NP_115883.2

ENSEMBL proteins:

ENSP00000386488 ENSP00000272570

Human Recombinant Proteins

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	Browse Abnova for Human Recombinant Proteins

OriGene Purified Recombinant Human Protein: ZC3H8

1 Gene Ontology (GO) cellular component term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	IEA	--

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Antibodies for ZC3H8:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse Antibodies at Sigma-Aldrich
	Browse R&D Systems for Antibodies
	Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
	Browse Abnova for Monoclonal and Polyclonal Antibodies
	Search Novus for antibodies for ZC3H8

Assays for ZC3H8:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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Graphical View of Domain Structure for InterPro Entry Q8N5P1

ProtoNet protein and cluster: Q8N5P1

1 Blocks protein family: IPB000571 Zn-finger

UniProtKB/Swiss-Prot: ZC3H8_HUMAN, Q8N5P1

Similarity: Contains 3 C3H1-type zinc fingers

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (ZC3H8)
		Browse for Gene Knock-down Tools from Millipore
		Abnova Chimera RNAi Products for Gene knock-down (ZC3H8)

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_032494

Applied Biosystems Silencer® siRNAs for ZC3H8

Sigma-Aldrich siRNA for ZC3H8

Sigma-Aldrich shRNA for ZC3H8

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Browse Clone Ranger at Invitrogen for clones
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_032494
Myc/DDK tagged cDNA clone in CMV expression vector: NM_032494
untagged cDNA clone in CMV expression vector: NM_032494

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_032494

3 Gene Ontology (GO) molecular function terms (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0003676	nucleic acid binding	IEA	--
GO:0008270	zinc ion binding	IEA	--
GO:0046872	metal ion binding	IEA	--

About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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2 Interacting proteins for ZC3H8 (ENSP00000272570³) via UniProtKB, MINT, and/or STRING

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
GATA3	ENSP00000368632³	STRING (score=.903)
ZNF266	ENSP00000354680³	STRING (score=.714)

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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for ZC3H8

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (ZC3H8)
		Browse for Gene Knock-down Tools from Millipore
		Abnova Chimera RNAi Products for Gene knock-down (ZC3H8)

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_032494

Sigma-Aldrich siRNA for ZC3H8

Sigma-Aldrich shRNA for ZC3H8

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_032494

REFSEQ mRNAs for ZC3H8 gene:

NM_032494.2

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_032494

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_032494
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_032494
                                 untagged cDNA clone in CMV expression vector: NM_032494

Additional cDNA sequence:

AF334161.1 AK315329.1 BC032001.1 CR590012.1

5 DOTS entries:

DT.407615 DT.95264603 DT.95264604 DT.100766708 DT.91742439

24/86 AceView cDNA sequences (see all 86 ):

BI907571 AA489020 AI288561 AA196768 CR590012 AA213370 BX119500 AI288563

AA316412 CB133959 AI160873 AA665159 AI631540 BU677501 BX500863 BF002258

AA912507 BX282716 AL707589 AA829479 AW187997 AA213369 BQ018359 AL530577

highest scoring ESTs for ZC3H8:

AF334161 AI631540 AI640199 AL707589 BC032001 CD104164 CF596791 AA101235 AA196768 AA213370

Unigene Cluster for ZC3H8:

Zinc finger CCCH-type containing 8

Hs.418416 [show with all ESTs]

Unigene Representative Sequence: BC032001

GeneLoc Exon Structure

5 Alternative Splicing Database (ASD) splice patterns (SP) for ZC3H8

ExUns:	1a	·	1b	·	1c	·	1d	^	2	^	3	^	4	^	5	^	6	^	7a	·	7b	^	8	^	9	^	10	^	11	^	12
SP1:
SP2:													-		-
SP3:							-		-				-		-
SP4:							-		-				-
SP5:							-		-

About this scheme

ECgene alternative splicing isoforms for ZC3H8

2 Ensembl transcripts including schematic representations:

ENST00000409573 ENST00000272570

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
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ZC3H8 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for ZC3H8

¹ / ² / ³

4 probe-sets matching ZC3H8 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
51700_at²^{, 3}	U95-B	1	1.00	1.00	0.62	0.44	AI631540	0.80	1.00	0.91	1
46633_at²^{, 3}	U95-B	1	1.00	1.00	1.00	1.00	AI640199	0.80	1.00	0.91	1
223506_at²^{, 3}	U133-B	1	1.00	1.00	--	--	AF334161	0.80	1.00	0.91	1
223506_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

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Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.418416

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for ZC3H8 gene from 5 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	ZC3H8¹	--	zinc finger CCCH-type containing 8	89.21(n) 87.54(a)	483063 XM_540178.2 XP_540178.2
chimpanzee (Pan troglodytes)	ZC3H8¹	--	zinc finger CCCH-type containing 8	98.97(n) 98.28(a)	459493 XM_515691.2 XP_515691.2
cow (Bos taurus)	ZC3H8¹	--	zinc finger CCCH-type containing 8	86.46(n) 81.25(a)	506983 NM_001075291.1 NP_001068759.1
rat (Rattus norvegicus)	Zc3h8¹	--	zinc finger CCCH type containing 8	81.9(n) 76.63(a)	311414 NM_001012090.1 NP_001012090.1
mouse (Mus musculus)	Zc3h8^{1, 5}	2⁵	zinc finger CCCH type containing 8^{1, 5}	83.73(n)¹ 78.69(a)¹	57432¹ NM_020594.2¹ NP_065619.2¹ AA171099⁵ AF061961⁵ (see all 14)

About this table Species with no ortholog for ZC3H8

ENSEMBL Gene Tree for ZC3H8

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for ZC3H8 gene

ZC3H4² ZC3H6²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/140 NCBI SNPs in ZC3H8 are shown (see all 140 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 40)

AB	SNP ID	Valid	Chr 2 pos	Sequence	Recs	AA Chg	Type	More	Recs	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--			--
	rs7571435^1,2	A,C,F,H	112730533(+)	`CAAAAG/TATAAC`	1	--	ng3¹		8	EU EA WA	838
	rs11691431^1,2	A,C,F,H	112730450(+)	`ACCCCC/TTCTTT`	1	--	ng3¹ trp³		8	EU EA WA	836
	rs10169925^1,2	A,C,F,H	112731076(+)	`gaaccG/Aaagac`	1	--	ng3¹		4	EU EA WA	418
	rs11674953^1,2	A,C,F,H	112730551(+)	`AAATGA/CAAATT`	1	--	ng3¹ tfbs³		8	EU EA WA	838
	rs7599551^1,2	C,F,H	112690395(+)	`cacatC/Tacccc`	1	--	ut3¹		8	EU EA WA	840
	rs35782954^1,2	C,F	112707321(+)	`TACAGA/CCAGTT`	1	C/W	mis¹		5	NA EU EA WA	472
	rs3749139^1,2	H	112729157(-)	`GGTGAG/AGCTGC`	1	--	ng3¹		4	EU EA WA	420
	rs7571959^1,2	H	112690196(+)	`accttG/Ccttcc`	1	--	ut3¹		4	EU EA WA	418
	rs13389045^1,2	C,H	112710688(+)	`ATTTTC/TTTCTG`	1	K/K	syn¹ ese³		4	EU EA WA	418
	rs1343666^1,2	A,C,F,H	112703426(-)	`AACTGC/GTAGAA`	1	--	int¹		16	EU EA WA NA	1472

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HapMap Linkage Disequilibrium images for ZC3H8 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
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5 PubMed articles for ZC3H8 gene:

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)^{2, 3} Strausberg R.L....Marra M.A. (2002)
A quantitative atlas of mitotic phosphorylation. (PubMed id 18669648)⁴ Dephoure N.... Gygi S.P. (2008)
ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. (PubMed id 17525332)⁴ Matsuoka S.... Elledge S.J. (2007)
Hepatitis C virus core protein interacts with 14-3-3 protein and activates the kinase Raf-1. (PubMed id )⁴ Aoki H.... Hino O. (2000)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 84524	HGNC: 30941	AceView: ZC3HDC8	Ensembl:ENSG00000144161	euGenes: HUgn84524
ECgene: ZC3H8	H-InvDB: ZC3H8

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
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Licensable Technologies for ZC3H8
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
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Products for ZC3H8:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for ZC3H8-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for ZC3H8		Browse antibodies at Sigma-Aldrich
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ZC3H8 Gene

protein-coding GIFtS: 46

Aliases &
Descriptions
for ZC3H8

Summaries
for ZC3H8

Genomic Location
for ZC3H8

Proteins
for ZC3H8

Protein Domains/
Families
for ZC3H8

Gene Function
for ZC3H8

Pathways & Interactions
for ZC3H8

Drugs & Compounds
for ZC3H8

Transcripts
for ZC3H8

Expression
for ZC3H8

Orthologs
for ZC3H8

Paralogs
for ZC3H8

SNPs/Variants
for ZC3H8

Disorders & Mutations
for ZC3H8

Medical News
for ZC3H8

Publications
for ZC3H8

Search for ZC3H8

Genome Databases showing ZC3H8

Other Databases showing ZC3H8

Specialized Databases showing ZC3H8

Licensable Technologies
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Services
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Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

ZC3H8 Gene

protein-coding GIFtS: 46

Aliases & Descriptionsfor ZC3H8

Summariesfor ZC3H8

Genomic Locationfor ZC3H8

Proteinsfor ZC3H8

Protein Domains/Familiesfor ZC3H8

Gene Functionfor ZC3H8

Pathways & Interactionsfor ZC3H8

Drugs & Compoundsfor ZC3H8

Transcriptsfor ZC3H8

Expression for ZC3H8

Orthologsfor ZC3H8

Paralogsfor ZC3H8

SNPs/Variantsfor ZC3H8

Disorders & Mutationsfor ZC3H8

Medical Newsfor ZC3H8

Publicationsfor ZC3H8

Search for ZC3H8

Genome Databases showing ZC3H8

Other Databases showing ZC3H8

Specialized Databases showing ZC3H8

Licensable Technologiesfor ZC3H8

Servicesfor ZC3H8

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for ZC3H8

Summaries
for ZC3H8

Genomic Location
for ZC3H8

Proteins
for ZC3H8

Protein Domains/
Families
for ZC3H8

Gene Function
for ZC3H8

Pathways & Interactions
for ZC3H8

Drugs & Compounds
for ZC3H8

Transcripts
for ZC3H8

Expression
for ZC3H8

Orthologs
for ZC3H8

Paralogs
for ZC3H8

SNPs/Variants
for ZC3H8

Disorders & Mutations
for ZC3H8

Medical News
for ZC3H8

Publications
for ZC3H8

Licensable Technologies
for ZC3H8

Services
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