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ZBTB33 Gene

protein-coding   GIFtS: 57

GC0XP119268
zinc finger and BTB domain containing 33
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
KAISO 1, 3, 5
OTTHUMP00000023935 2
WUGSC:H_DJ525N14.1 1, 2
ZNF-kaiso 1, 2
ZNF348 1, 2, 3
kaiso 1, 2
Descriptions
Zinc finger and BTB domain-containing protein 33 3
kaiso transcription factor 2
zinc finger and BTB domain containing 33 2
External Ids
HGNC: 166821
Entrez Gene: 100092
UniProtKB: Q86T243
Ensembl: ENSG000001774857
Search outside databases for aliases for ZBTB33 gene

Previous GC identifers: GC0XP118139 GC0XP119166

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for ZBTB33:
KAISO is a transcriptional regulator that binds, via its zinc fingers, to DNA sequences containing
methylated CGCG or to the consensus KAISO-binding site (KBS) TCCTGCNA (Filion et al., 2006 [PubMed
16354688]).[supplied by OMIM]

UniProtKB/Swiss-Prot: KAISO_HUMAN, Q86T24
Function: Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG
dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus
sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and
the formation of repressive chromatin structures in target gene promoters. May contribute to the
repression of target genes of the Wnt signaling pathway. May also activate transcription of a
subset of target genes by the recruitment of CTNND2

Gene Wiki entry for ZBTB33

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the ZBTB33 gene  

Entrez Gene cytogenetic band: Xq23   Ensembl cytogenetic band:  Xq24   HGNC cytogenetic band: Xq23

ZBTB33 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome X         GeneLoc Exon Structure

GeneLoc location for GC0XP119268:     (about GC identifiers)

Start:
119,268,635 bp from pter
End:
119,276,279 bp from pter
Size:
7,645 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000023.9  NT_011786.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: KAISO_HUMAN, Q86T24 (See protein sequence)
Recommended Name: Transcriptional regulator Kaiso  
Size: 672 amino acids; 74484 Da
Subunit: Self-associates. Interacts with CTNND2 (By similarity). Interacts with CTNND1, and this
interaction inhibits binding to both methylated and non-methylated DNA. Interacts with NCOR1.
Interacts with KPNA2/RCH1, which may mediate nuclear import of this protein
Subcellular location: Nucleus. Cytoplasm. Note=Also cytoplasmic in cells grown at high densities
PDB structures from and Proteopedia :
3FKC (3D)    
Secondary accessions: O00319 Q7Z361 Q8IVP6 Q8N3P0

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_006768.1  

    ENSEMBL proteins: 
    ENSP00000314153 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Kaiso)
    Human Recombinant Proteins from Abnova (ZBTB33)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    5 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005622 intracellular IEA--
    GO:0005634 nucleus TAS10207085
    GO:0005730 nucleolus IDA18029348
    GO:0005737 cytoplasm IEA--
    GO:0005886 plasma membrane IDA18029348
    About this table

    Antibodies for ZBTB33: 
    Invitrogen Antibodies for ZBTB33
    Millipore Mono- and Polyclonal Antibodies for the study of ZBTB33
    Sigma-Aldrich Antibody Arrays and Antibodies for ZBTB33
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (Kaiso), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (ZBTB33)
    Novus Biologicals Antibodies for ZBTB33

    Assays for ZBTB33: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5 InterPro domains/families:
     IPR000210 BTB/POZ-like
     IPR015880 Znf_C2H2-like
     IPR011333 BTB/POZ_fold
     IPR013069 BTB_POZ
     IPR007087 Znf_C2H2


       GeneDecks  ZBTB33 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q86T24

    ProtoNet protein and cluster: Q86T24

    1 Blocks protein family: IPB000210 BTB/POZ domain

    UniProtKB/Swiss-Prot: KAISO_HUMAN, Q86T24
    Similarity: Contains 1 BTB (POZ) domain
    Similarity: Contains 3 C2H2-type zinc fingers

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (ZBTB33)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (ZBTB33)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006777

                  Applied Biosystems Silencer® siRNAs for ZBTB33

                  Sigma-Aldrich siRNA and siRNA Panels for ZBTB33  
                         Sigma-Aldrich shRNA for ZBTB33  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for ZBTB33
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006777
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006777
                                     untagged cDNA clones in CMV expression vector (see all 2): AK092998 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_006777

    UniProtKB/Swiss-Prot: KAISO_HUMAN, Q86T24
    Function: Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG
    dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus
    sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and
    the formation of repressive chromatin structures in target gene promoters. May contribute to the
    repression of target genes of the Wnt signaling pathway. May also activate transcription of a
    subset of target genes by the recruitment of CTNND2
    Induction: Induced in vascular endothelium by wounding. This effect is potentiated by prior laminar
    shear stress, which enhances wound closure

    4 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Zbtb33):

    life span-post-weaning/agingno phenotypic analysisnormaltumorigenesis

    4 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003677 DNA binding IEA--
    GO:0005515 protein binding TAS10207085
    GO:0008270 zinc ion binding IEA--
    GO:0046872 metal ion binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


     Gene Network CentralTM Interacting Genes and Proteins Network for  ZBTB33 


    5/6 Interacting proteins for ZBTB33 (ENSP000003141533) via UniProtKB, MINT, and/or STRING (see all 6 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CTNND1ENSP000003548233STRING (score=.996)
    MMP7ENSP000002602273STRING (score=.939)
    DACT1ENSP000003374393STRING (score=.812)
    CTNNB1ENSP000003444563STRING (score=.736)
    NCOR1ENSP000002687123STRING (score=.724)
    About this table

    4 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006350 transcription IEA--
    GO:0006355 regulation of transcription, DNA-dependent IEA--
    GO:0007242 intracellular signaling cascade TAS10207085
    GO:0016055 Wnt receptor signaling pathway IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for ZBTB33

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (ZBTB33)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (ZBTB33)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006777

                  Sigma-Aldrich siRNA and siRNA Panels for ZBTB33  
                         Sigma-Aldrich shRNA for ZBTB33  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_006777  

    REFSEQ mRNAs for ZBTB33 gene: 

    NM_006777.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_006777  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006777
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006777
                                     untagged cDNA clones in CMV expression vector (see all 2): AK092998 

    Additional cDNA sequence: 

    AK092998.1 AK094230.1 AK312321.1 AL833604.1 AL833856.1 BC042753.1 BX538016.1 BX538101.1 

    6 DOTS entries:

    DT.413928  DT.121301268  DT.121301263  DT.100721641  DT.121301271  DT.95237159 

    24/121 AceView cDNA sequences (see all 121 ):

    BX955913 BF971259 BQ186564 AA293205 T61211 BE018480 BF512729 CF593941 
    BF514257 BQ945983 BX092275 AL833856 NM_006777 BU172925 CB148666 AA482006 
    BP335246 BE302089 AA279178 AK094230 BE790431 AA443179 BC042753 AA279190 

    highest scoring ESTs for ZBTB33:

    AA521039 AA279178 AA279190 AA293205 AA293206 AA354830 AA360877 AA419400 AA443179 AA463633 

    Unigene Cluster for ZBTB33:

    Zinc finger and BTB domain containing 33
    Hs.143604  [show with all ESTs]
    Unigene Representative Sequence: BC042753


    GeneLoc Exon Structure

    2 Alternative Splicing Database (ASD) splice patterns (SP) for ZBTB33

    ExUns: 1a · 1b ^ 2 ^ 3a · 3b
    SP1:                              
    SP2:              -               

    About this scheme

    ECgene alternative splicing isoforms for ZBTB33

    1 Ensembl transcript including schematic representation:
    ENST00000326624  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    ZBTB33 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for ZBTB33

    1 / 2 / 3

    6 probe-sets matching ZBTB33 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    58520_at2, 3 U95-C 1 1.00 1.00 0.73 1.52 AA521039 1.00 1.00 1.00 1

    40178_at2, 3 U95-A 1 1.00 1.00 0.86 0.49 AC002086 0.20 1.00 0.72 1

    226255_at2, 3 U133-B 1 1.00 1.00 -- -- BE302089 0.80 1.00 0.91 1

    214631_at2, 3 U133-A 1 0.91 1.00 -- -- BG391005 0.80 1.00 0.91 1

    226255_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    214631_at2 U133Plus2 1 0.91 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GCCTGATATT

    SOURCE GeneReport for Unigene cluster: Hs.143604

    Expression variation in blood from EXPOLDB for ZBTB33

    UniProtKB/Swiss-Prot: KAISO_HUMAN, Q86T24
    Tissue specificity: Expressed in vascular endothelium

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for ZBTB33 gene from 5/6 species (see all 6 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    chimpanzee
    (Pan troglodytes)
    ZBTB331   -- zinc finger and BTB domain containing 33 99.85(n)
    99.85(a)
    465831  XM_521239.2  XP_521239.2 
    rat
    (Rattus norvegicus)
    Zbtb331   -- zinc finger and BTB domain containing 33 88.37(n)
    87.87(a)
    501506  XM_576906.2  XP_576906.1 
    mouse
    (Mus musculus)
    Zbtb331, 5 X5
    zinc finger and BTB domain containing 331, 5 89.69(n)1
    88.64(a)1
    568051  NM_001079513.11  NP_001072981.11 
     AF0974165  AK0803015  (see all 17)
    chicken
    (Gallus gallus)
    ZBTB331   -- zinc finger and BTB domain containing 33 67.31(n)
    63.98(a)
    422367  XM_420339.2  XP_420339.2 
    zebrafish
    (Danio rerio)
    zbtb331   -- zinc finger and BTB domain containing 33 54.92(n)
    48.99(a)
    562507  NM_001100008.1  NP_001093478.1 
    About this table        Species with no ortholog for ZBTB33

    ENSEMBL Gene Tree for ZBTB33
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for ZBTB33 gene
    ZNF2952  ZBTB382  ZFP1612  ZBTB42  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/23 NCBI SNPs in ZBTB33 are shown (see all 23 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 19)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr X posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs10444131,2
    A,C,F,H119275908(+) CTTGCA/GAGACT 1 -- ut31 ese37Minor allele frequency- G:0.47EU EA WA NA 550
    rs78824801,2
    A,C,F,H119273994(+) ACCTTT/CGCAGT 1 -- ut31 ese311Minor allele frequency- C:0.33NA EU EA WA 770
    --
    rs59108101,2
    H119274693(+) AAAGGA/TAAAAA 1 -- ut31 ese3 trp34Minor allele frequency- T:0.00EU EA WA 384
    rs22854061,2
    H119272345(+) TCGGCC/TGTCAG 1 A/A syn1 ese34Minor allele frequency- T:0.00EU EA WA 416
    rs169801731,2
    F,H119271176(+) AATTAT/GGCTGA 1 -- int17Minor allele frequency- G:0.05NA EU EA WA 514
    rs70514961,2
    H119269207(+) ATTTAG/AAGCTT 1 -- int14Minor allele frequency- A:0.00EU EA WA 418
    --
    rs113476761,2
    --119273547(+) GCTCA-/TTTAAA 1 -- ut310--------
    --
    rs724132321,2
    --119271862(+) ATTCT-/TGAGATGA 1 S/SD fra10--------
    --
    rs679984821,2
    --119273729(+) AATAAA/GTGTGT 1 -- ut310--------
    --
    rs596773031,2
    --119266981(+) TTTTT-/TACTAC 1 -- ng510--------
    About this table

    HapMap Linkage Disequilibrium images for ZBTB33 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 300329

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/27 PubMed articles for ZBTB33 gene (see all 27 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 10009 HGNC: 16682 AceView: ZBTB33 Ensembl:ENSG00000177485 euGenes: HUgn10009
    ECgene: ZBTB33 H-InvDB: ZBTB33
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for ZBTB33:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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     Predesigned and custom siRNAs for ZBTB33 Antibodies for ZBTB33
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for ZBTB33 Browse small molecules at Sigma-Aldrich
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