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ZBTB32 Gene

protein-coding   GIFtS: 54

GC19P040896
zinc finger and BTB domain containing 32
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
FAXF 1, 2, 5
FAZF 1, 2, 3
OTTHUMP00000045790 2
ROG 5
Rog 1, 2
TZFP 1, 2, 3, 5
ZNF538 1, 2
Descriptions
FANCC-interacting protein 3
Fanconi anemia zinc finger protein 3
Testis zinc finger protein 2, 3
repressor of GATA 2
zinc finger and BTB domain containing 32 2
External Ids
HGNC: 167631
Entrez Gene: 270332
UniProtKB: Q9Y2Y43
Ensembl: ENSG000000115907
Search outside databases for aliases for ZBTB32 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: ZBT32_HUMAN, Q9Y2Y4
Function: DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function
as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor
effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation
and activation of helper T cells (By similarity)

Gene Wiki entry for ZBTB32

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the ZBTB32 gene  

Entrez Gene cytogenetic band: 19q13.1   Ensembl cytogenetic band:  19q13.12   HGNC cytogenetic band: 19q13.1

ZBTB32 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 19         GeneLoc Exon Structure

GeneLoc location for GC19P040896:     (about GC identifiers)

Start:
40,895,670 bp from pter
End:
40,899,780 bp from pter
Size:
4,111 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000019.8  NT_011109.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: ZBT32_HUMAN, Q9Y2Y4 (See protein sequence)
Recommended Name: Zinc finger and BTB domain-containing protein 32  
Size: 487 amino acids; 52963 Da
Subunit: Interacts with the N-terminal of FANCC. Interacts with ZBTB16. Interacts with GATA3 (By
similarity)
Subcellular location: Nucleus. Note=Located in nuclear speckles
Secondary accessions: Q8WVP2

REFSEQ proteins: NP_055198.1  

ENSEMBL proteins: 
ENSP00000345954 ENSP00000376035 ENSP00000262630 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (TZFP)
Human Recombinant Proteins from Abnova (ZBTB32)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

3 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000228 nuclear chromosome IEA--
GO:0005622 intracellular IEA--
GO:0005634 nucleus TAS10572087
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Antibodies for ZBTB32: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for ZBTB32
Browse R&D Systems for Antibodies
Antibodies from Abcam (TZFP), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (ZBTB32)
Novus Biologicals Antibodies for ZBTB32

Assays for ZBTB32: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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5/6 InterPro domains/families (see all 6 ):
 IPR000210 BTB/POZ-like
 IPR015880 Znf_C2H2-like
 IPR011333 BTB/POZ_fold
 IPR013087 Znf_C2H2/integrase_DNA-bd
 IPR013069 BTB_POZ


   GeneDecks  ZBTB32 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q9Y2Y4

ProtoNet protein and cluster: Q9Y2Y4

1 Blocks protein family: IPB000210 BTB/POZ domain

UniProtKB/Swiss-Prot: ZBT32_HUMAN, Q9Y2Y4
Domain: The C-terminal zinc finger domain functions as a transcriptional transactivator (By
similarity)
Domain: The BTB (POZ) domain possesses repressor activity (By similarity)
Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family
Similarity: Contains 1 BTB (POZ) domain
Similarity: Contains 3 C2H2-type zinc fingers

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (ZBTB32)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (ZBTB32)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_014383

              Applied Biosystems Silencer® siRNAs for ZBTB32

              Sigma-Aldrich siRNA and siRNA Panels for ZBTB32  
                     Sigma-Aldrich shRNA for ZBTB32  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for ZBTB32
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_014383
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_014383
                                 untagged cDNA clone in CMV expression vector: NM_014383 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_014383

UniProtKB/Swiss-Prot: ZBT32_HUMAN, Q9Y2Y4
Function: DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function
as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor
effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation
and activation of helper T cells (By similarity)

3 MGI mutant phenotypes (inferred from 6 alleles(MGI details for Zbtb32):

hematopoietic systemimmune systemnormal

5/6 Gene Ontology (GO) molecular function terms (links to tree view) (see all 6 ):

GO IDQualified GO termEvidencePubMed IDs
GO:0003677 DNA binding IEA--
GO:0003704 specific RNA polymerase II transcription factor activity TAS10572087
GO:0003714 transcription corepressor activity TAS10572087
GO:0005515 protein binding TAS10572087
GO:0008270 zinc ion binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section



5/12 Interacting proteins for ZBTB32 (Q9Y2Y42 ENSP000002626303) via UniProtKB, MINT, and/or STRING (see all 12 )
InteractantInteraction Details
GeneCardExternal ID(s)
ATXN1P542532MINT-2872096
RHOXF2Q9BQY42MINT-67810
GATA3ENSP000003686323STRING (score=.987)
FANCCENSP000003644543STRING (score=.984)
TBX21ENSP000001776943STRING (score=.893)
About this table

5/6 Gene Ontology (GO) biological process terms (links to tree view) (see all 6 ):

GO IDQualified GO termEvidencePubMed IDs
GO:0000122 negative regulation of transcription from RNA polymerase II promoter TAS10572087
GO:0001817 regulation of cytokine production IEA--
GO:0006350 transcription IEA--
GO:0006355 regulation of transcription, DNA-dependent IEA--
GO:0030097 hemopoiesis IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for ZBTB32
1 Novoseek chemical compound relationship for ZBTB32 gene
Compound   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
zinc 53.06 7 10544010 (1), 11865059 (1), 11964310 (1)
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(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (ZBTB32)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (ZBTB32)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_014383

              Sigma-Aldrich siRNA and siRNA Panels for ZBTB32  
                     Sigma-Aldrich shRNA for ZBTB32  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_014383  

REFSEQ mRNAs for ZBTB32 gene: 

NM_014383.1   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_014383  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_014383
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_014383
                                 untagged cDNA clone in CMV expression vector: NM_014383 

Additional cDNA sequence: 

AF130255.1 AF165097.1 BC017700.2 

4 DOTS entries:

DT.208787  DT.91890193  DT.100737697  DT.91813092 

24/41 AceView cDNA sequences (see all 41 ):

NM_014383 BE674864 H48938 BC017700 BM563565 AF130255 BX095236 AF165097 
AI807480 BI825896 BI825319 AI150041 AI540057 BF980407 BG177331 BG724348 
BI828090 AI150330 BI826750 BI826880 BG717422 AI223414 BG771476 AI798147 

highest scoring ESTs for ZBTB32:

AI798147 AA456628 AA476210 AA868505 AA927876 AF130255 AF165097 AI150041 AI150330 AI223414 

Unigene Cluster for ZBTB32:

Zinc finger and BTB domain containing 32
Hs.99430  [show with all ESTs]
Unigene Representative Sequence: NM_014383


GeneLoc Exon Structure

4 Alternative Splicing Database (ASD) splice patterns (SP) for ZBTB32

ExUns: 1a · 1b ^ 2a · 2b ^ 3 ^ 4 ^ 5a · 5b ^ 6a · 6b
SP1:                                      -                     
SP2:              -           -           -                     
SP3:                          -     -     -                     
SP4:                                                            

About this scheme

ECgene alternative splicing isoforms for ZBTB32

3 Ensembl transcripts including schematic representations:
ENST00000354109  ENST00000392197  ENST00000262630  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

ZBTB32 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for ZBTB32

1 / 2 / 3

4 probe-sets matching ZBTB32 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
58391_at2, 3 U95-C 1 1.00 1.00 0.53 1.16 AI798147 1.00 1.00 1.00 1

70837_at2, 3 U95-E 1 0.38 1.00 0.36 0.89 AI807480 0.60 1.00 0.82 1

220118_at2, 3 U133-A 1 1.00 1.00 -- -- NM_014383 0.60 1.00 0.82 1

220118_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  ZBTB32 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: CGCGGTCACT

SOURCE GeneReport for Unigene cluster: Hs.99430

UniProtKB/Swiss-Prot: ZBT32_HUMAN, Q9Y2Y4
Tissue specificity: Predominantly expressed in testis. Some isoforms are ubiquitously expressed

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for ZBTB32 gene from 5 species
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
ZBTB321   -- zinc finger and BTB domain containing 32 84.95(n)
79.7(a)
484577  XM_541691.2  XP_541691.2 
chimpanzee
(Pan troglodytes)
ZBTB321   -- zinc finger and BTB domain containing 32 99.18(n)
98.56(a)
745538  XM_001160714.1  XP_001160714.1 
cow
(Bos taurus)
ZBTB321   -- zinc finger and BTB domain containing 32 84.84(n)
76.95(a)
785741  XM_001253667.1  XP_001253668.1 
rat
(Rattus norvegicus)
Zbtb321   -- zinc finger and BTB domain containing 32 77.43(n)
70.65(a)
688845  XM_001068541.1  XP_001068541.1 
mouse
(Mus musculus)
Zbtb321, 5 75
zinc finger and BTB domain containing 321, 5 77.22(n)1
72.89(a)1
582061  NM_021397.21  NP_067372.21 
 AF2412325  AK0158815  (see all 10)
About this table        Species with no ortholog for ZBTB32

ENSEMBL Gene Tree for ZBTB32
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
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Paralogs for ZBTB32 gene
ZBTB392  ZBTB162  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/33 NCBI SNPs in ZBTB32 are shown (see all 33 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 40)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 19 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs22343601,2
C,F,H,O40894884(+) GTGTAT/CCTAAT 1 -- ng518Minor allele frequency- C:0.09EU EA WA 838
rs22272781,2
A,C,F,H40897890(+) GGCAGA/CCCCGA 1 R/S mis1 ese39Minor allele frequency- C:0.10EU EA WA NA 910
rs37870521,2
C,F,H40893895(-) CAGCTC/TCCCCG 1 -- ng519Minor allele frequency- T:0.11EA EU WA 2318
--
rs22343611,2
C,H40895167(+) CCACTG/AGGCAA 1 -- ng514Minor allele frequency- A:0.00EU EA WA 392
rs22343651,2
C40897535(+) GGGCCG/TCAGGG 1 R/L mis1 ese31Minor allele frequency- T:0.01NA 78
rs22343631,2
C40895274(+) ACAGAA/TAAAGA 1 -- ng51 tfbs3 trp30--------
rs22343641,2
H40895495(+) TCAGAG/ACTTGA 1 -- ng514Minor allele frequency- A:0.00EU EA WA 416
rs1070681,2
A,C,F,H,O40896530(+) TACCCA/GAATCT 1 -- int121Minor allele frequency- N:0.00EA NA MN EU WA 1776
--
rs235951,2
A,C,F40896671(-) GAGAAA/C/GGAGGA 1 -- int1 trp31NA 12
rs37462811,2
C,F,H40895998(-) CCCCTC/TTACCT 1 -- int15Minor allele frequency- T:0.04EA EU WA 1890
About this table

HapMap Linkage Disequilibrium images for ZBTB32 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 605859

1 Novoseek disease relationship for ZBTB32 gene

Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
fanconis anemia 73.70 3 11865059 (1), 11964310 (1)
About this table

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/14 PubMed articles for ZBTB32 gene (see all 14 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 27033 HGNC: 16763 AceView: TZFP Ensembl:ENSG00000011590 euGenes: HUgn27033
ECgene: ZBTB32 H-InvDB: ZBTB32
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for ZBTB32:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
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  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for ZBTB32-related products

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 Predesigned and custom siRNAs for ZBTB32 Antibodies for ZBTB32
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for ZBTB32 Browse small molecules at Sigma-Aldrich
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 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Antibodies for ZBTB32

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