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ZBP1 Gene

protein-coding   GIFtS: 56

GC20M055602
Z-DNA binding protein 1
(Previous name: chromosome 20 open reading frame 183 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: C20orf183)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
C20ORF183 5
C20orf183 2, 3
DAI 2
DLM-1 1, 2
DLM1 1, 2, 3, 5
OTTHUMP00000031373 2
dJ718J7.3 1
Descriptions
DNA-dependent activator of interferon regulatory factors 2
Tumor stroma and activated macrophage protein DLM-1 2, 3
Z-DNA binding protein 1 2
chromosome 20 open reading frame 183 1
External Ids
HGNC: 161761
Entrez Gene: 810302
UniProtKB: Q9H1713
Ensembl: ENSG000001242567
Search outside databases for aliases for ZBP1 gene

Previous GC identifers: GC20M055907 GC20M056817 GC20M056864

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for ZBP1:
DLM1 encodes a Z-DNA binding protein. Z-DNA formation is a dynamic process, largely controlled by
the amount of supercoiling.[supplied by OMIM]

UniProtKB/Swiss-Prot: ZBP1_HUMAN, Q9H171
Function: May play a role in host defense against tumors and pathogens. Binds Z-DNA (By similarity)

Gene Wiki entry for ZBP1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the ZBP1 gene  

Entrez Gene cytogenetic band: 20q13.31   Ensembl cytogenetic band:  20q13.31   HGNC cytogenetic band: 20q13.31

ZBP1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 20         GeneLoc Exon Structure

GeneLoc location for GC20M055602:     (about GC identifiers)

Start:
55,612,308 bp from pter
End:
55,629,038 bp from pter
Size:
16,731 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000020.9  NT_011362.9  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: ZBP1_HUMAN, Q9H171 (See protein sequence)
Recommended Name: Z-DNA-binding protein 1  
Size: 429 amino acids; 46343 Da
PDB structures from and Proteopedia :
3EYI (3D)    
Secondary accessions: Q5JY39 Q9BYW4
Alternative splicing: 5 isoforms:  Q9H171-1   Q9H171-2   Q9H171-3   Q9H171-4   Q9H171-5   (Additional isoforms seem to exist)

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (4 alternative transcripts): 
    NP_001153889.1  NP_001153890.1  NP_001153891.1  NP_110403.2  


    ENSEMBL proteins: 
    ENSP00000340584 ENSP00000379167 ENSP00000350306 ENSP00000360215 ENSP00000344954 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Z DNA binding protein)
    Human Recombinant Proteins from Abnova (ZBP1)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005575 cellular_component ND--
    About this table

    Antibodies for ZBP1: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for ZBP1
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (Z DNA binding protein), each with their AbpromiseSM
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for ZBP1

    Assays for ZBP1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    2 InterPro domains/families:
     IPR000607 dsRNA_A_deaminase
     IPR011991 Wing_hlx_DNA_bd


       GeneDecks  ZBP1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9H171

    ProtoNet protein and cluster: Q9H171

    1 Blocks protein family: IPB000607 Double-stranded RNA-specific adenosine deaminase (DRADA)

    UniProtKB/Swiss-Prot: ZBP1_HUMAN, Q9H171
    Similarity: Contains 2 DRADA repeats

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (ZBP1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (ZBP1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_030776

                  Applied Biosystems Silencer® siRNAs for ZBP1

                  Sigma-Aldrich siRNA for ZBP1  
                         Sigma-Aldrich shRNA for ZBP1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for ZBP1
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_030776
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_030776
                                     untagged cDNA clones in CMV expression vector (see all 2): BC028218 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_030776

    UniProtKB/Swiss-Prot: ZBP1_HUMAN, Q9H171
    Function: May play a role in host defense against tumors and pathogens. Binds Z-DNA (By similarity)

    Genatlas biochemistry entry for ZBP1:
    beta-actin mRNA zipcode binding protein,68kDa,,some homology with KOC,possibly involved in
    localization of beta-actin at the leading edge of fibroblasts

    1 MGI mutant phenotype (inferred from 1 allele(MGI details for Zbp1):

    immune system

    4 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003677 DNA binding IEA--
    GO:0003692 left-handed Z-DNA binding NAS11842111
    GO:0003723 RNA binding IEA--
    GO:0003726 double-stranded RNA adenosine deaminase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Sigma-Aldrich "Your Favorite Gene" Pathway for  ZBP1  (Your Favorite Gene powered by Ingenuity) 
     Activation of IRF by Cytosolic Pattern Recognition Receptors

       GeneDecks  ZBP1 for the pathways selected above  
    About GeneDecksing

     Gene Network CentralTM Interacting Genes and Proteins Network for  ZBP1 


    5/13 Interacting proteins for ZBP1 (Q9H1712 ENSP000003405843) via UniProtKB, MINT, and/or STRING (see all 13 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    DDB1Q165312MINT-6167588 MINT-6167618
    DDX5P178442MINT-6167588 MINT-6167618
    HNRNPA1P096512MINT-6167588 MINT-6167618
    HNRNPA1P2P096512MINT-6167588 MINT-6167618
    ILF3Q129062MINT-6167588 MINT-6167618
    About this table

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0008150 biological_process ND--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for ZBP1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (ZBP1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (ZBP1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_030776

                  Sigma-Aldrich siRNA for ZBP1  
                         Sigma-Aldrich shRNA for ZBP1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_030776  

    REFSEQ mRNAs for ZBP1 gene (4 alternative transcripts): 

    NM_001160417.1   NM_001160418.1   NM_001160419.1   NM_030776.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_030776  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_030776
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_030776
                                     untagged cDNA clones in CMV expression vector (see all 2): BC028218 

    Additional cDNA sequence: 

    AJ300575.1 AK122761.1 AK128405.1 AK290918.1 AK314062.1 BC028218.1 BC131706.1 

    6 DOTS entries:

    DT.91863900  DT.75126443  DT.100738532  DT.101980400  DT.100738531  DT.120813261 

    21 AceView cDNA sequences:

    BC028218 BF507915 AX775975 AJ300575 AW085614 AK122761 AI659939 NM_030776 
    AX775973 AW449265 AK128405 BM681709 BI910829 BX280705 N23727 CA433778 
    BM727331 BF511589 AW401499 AI925506 BQ319297 

    highest scoring ESTs for ZBP1:

    AJ300575 AK122761 BM727331 AI659939 AK128405 AW085614 AW401499 BC028218 BI910829 NM_030776 

    Unigene Cluster for ZBP1:

    Z-DNA binding protein 1
    Hs.302123  [show with all ESTs]
    Unigene Representative Sequence: AK128405


    GeneLoc Exon Structure

    5/9 Alternative Splicing Database (ASD) splice patterns (SP) for ZBP1 (see all 9 )

    ExUns: 1a · 1b · 1c · 1d · 1e · 1f ^ 2a · 2b ^ 3 ^ 4 ^ 5a · 5b · 5c ^ 6a · 6b ^ 7 ^ 8 ^ 9 ^ 10a · 10b ^ 11 ^ 12
    SP1:                                -     -                 -                             -           -                                 
    SP2:                                -     -     -           -                             -           -                                 
    SP3:                                                        -                             -                                             
    SP4:                                -     -                 -                                                                           
    SP5:                                      -                 -                                                                           

    About this scheme

    ECgene alternative splicing isoforms for ZBP1

    5 Ensembl transcripts including schematic representations:
    ENST00000343535  ENST00000395822  ENST00000357677  ENST00000371173  ENST00000340462  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    ZBP1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for ZBP1

    1 / 2 / 3

    5 probe-sets matching ZBP1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    88416_at2, 3 U95-D 1 0.94 1.00 1.00 1.00 AI659939 0.60 1.00 0.82 1

    242020_s_at2, 3 U133-B 1 1.00 1.00 -- -- AI925506 0.40 1.00 0.76 1

    208087_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_030776 0.60 1.00 0.82 1

    242020_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    208087_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  ZBP1 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: CTAGGGAGAC

    SOURCE GeneReport for Unigene cluster: Hs.302123

    UniProtKB/Swiss-Prot: ZBP1_HUMAN, Q9H171
    Tissue specificity: Highly expressed in lymphatic tissues including lymph node, leukocytes, tonsil,
    bone marrow and spleen. Expressed to a lesser extent in thymus, lung and liver

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for ZBP1 gene from 4 species
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    chimpanzee
    (Pan troglodytes)
    ZBP11   -- Z-DNA binding protein 1 98.91(n)
    98.14(a)
    746148  XM_001170447.1  XP_001170447.1 
    cow
    (Bos taurus)
    ZBP11   -- Z-DNA binding protein 1 68.79(n)
    55.73(a)
    508333  XM_585095.3  XP_585095.2 
    rat
    (Rattus norvegicus)
    Zbp11   -- Z-DNA binding protein 1 62.98(n)
    50.64(a)
    171091  XM_342594.2  XP_342595.1 
    mouse
    (Mus musculus)
    Zbp11, 5 25
    Z-DNA binding protein 11, 5 65(n)1
    51.84(a)1
    582031  NM_021394.11  NP_067369.11 
     AF1365205  AI6438865  (see all 12)
    About this table        Species with no ortholog for ZBP1

    ENSEMBL Gene Tree for ZBP1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/168 NCBI SNPs in ZBP1 are shown (see all 168 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 78)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 20 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs20731451,2
    A,C,F,H55624040(-) CAGCCA/GAGAGG 1 E/K mis1 ese315Minor allele frequency- N:0.00EA NA EU WA 2702
    rs60645721,2
    A,C,F,H55612828(+) GGCAAC/ATCCCT 1 -- ut317Minor allele frequency- A:0.32EU EA WA NA 562
    rs169811871,2
    C,F,H55623391(+) CATGTC/GCAGAA 1 D/H mis1 ese312Minor allele frequency- G:0.10NA EU EA WA 1042
    --
    rs81164201,2
    A,C,F55612018(+) tataaA/Taaaat 1 -- ng513Minor allele frequency- T:0.07NA 134
    rs28653941,2
    C,H55620290(+) TGGACC/TGCTCC 1 R/Q mis1 ese35Minor allele frequency- T:0.00EU EA WA NA 508
    rs344789441,2
    C,F55623354(-) TTACCG/ACCCAG 1 H/R mis15Minor allele frequency- A:0.02NA EU EA WA 496
    rs349646091,2
    C,F55624757(-) TGGGCG/AGGACT 1 R/G mis11Minor allele frequency- A:0.03NA 78
    --
    rs48118911,2
    A55629576(+) tcaagC/Tgatct 1 -- ng310--------
    rs358131251,2
    F55624915(-) ACAAAG/TAATCC 1 R/I mis11Minor allele frequency- T:0.03NA 74
    --
    rs48118851,2
    A55612334(+) TTTATG/TTATTT 1 -- ut31 ese30--------
    About this table

    HapMap Linkage Disequilibrium images for ZBP1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 606750

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/15 PubMed articles for ZBP1 gene (see all 15 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 81030 HGNC: 16176 AceView: ZBP1 Ensembl:ENSG00000124256 euGenes: HUgn81030
    ECgene: ZBP1 H-InvDB: ZBP1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for ZBP1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
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     Millipore Target Screening & Profiling Services


     Predesigned and custom siRNAs for ZBP1 Antibodies for ZBP1
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for ZBP1 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Recombinant Proteins
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