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PTGR2 Gene

protein-coding   GIFtS: 44

GC14P073389
prostaglandin reductase 2
(Previous name: zinc binding alcohol dehydrogenase domain containing 1 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: ZADH1)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
15-oxoprostaglandin-delta13-reductase 2
DKFZp686P10120 2
EC 1.3.1.48 3
FLJ39091 1, 2
FLJ99229 2
PGR2 2
PRG-2 3
ZADH1 2, 3
Descriptions
15-oxoprostaglandin 13-reductase 3
Zinc-binding alcohol dehydrogenase domain-containing
protein 1 3
prostaglandin reductase 2 2
zinc binding alcohol dehydrogenase domain containing 1 1
zinc binding alcohol dehydrogenase, domain containing 1 2
External Ids
HGNC: 201491
Entrez Gene: 1454822
UniProtKB: Q8N8N73
Ensembl: ENSG000001400437
Search outside databases for aliases for PTGR2 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for PTGR2:
This gene encodes an enzyme involved in the metabolism of prostaglandins. The encoded protein
catalyzes the NADPH-dependent conversion of 15-keto-prostaglandin E2 to
15-keto-13,14-dihydro-prostaglandin E2. This protein may also be involved in regulating activation
of the peroxisome proliferator-activated receptor. Alternative splicing results in multiple
transcript variants. [provided by RefSeq]

UniProtKB/Swiss-Prot: PTGR2_HUMAN, Q8N8N7
Function: Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2,
15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest activity towards 15-keto-PGE2.
Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation
(By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 14q24.3   Ensembl cytogenetic band:  14q24.3   HGNC cytogenetic band: 14q24.1-q24.2

PTGR2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 14         GeneLoc Exon Structure

GeneLoc location for GC14P073389:     (about GC identifiers)

Start:
73,388,381 bp from pter
End:
73,421,915 bp from pter
Size:
33,535 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000014.7  NT_026437.11  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: PTGR2_HUMAN, Q8N8N7 (See protein sequence)
Recommended Name: Prostaglandin reductase 2  
Size: 351 amino acids; 38499 Da
Cofactor: NADPH (By similarity)
Subunit: Monomer (By similarity)
Subcellular location: Cytoplasm (By similarity)
PDB structures from and Proteopedia :
2VNA (3D)    2W4Q (3D)    2W98 (3D)    2ZB4 (3D)    2ZB7 (3D)    2ZB8 (3D)    
Secondary accessions: Q3L8A4 Q6MZH8
Alternative splicing: 2 isoforms:  Q8N8N7-1   Q8N8N7-2   

REFSEQ proteins (3 alternative transcripts): 
NP_001139626.1  NP_001139627.1  NP_689657.1  


ENSEMBL proteins: 
ENSP00000384663 ENSP00000267568 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (ZADH1)
Human Recombinant Proteins from Abnova (ZADH1)
              OriGene Purified Recombinant Human Protein: PTGR2 

3 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005634 nucleus IDA18029348
GO:0005730NOT nucleolus IDA18029348
GO:0005737 cytoplasm NAS--
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Antibodies for PTGR2: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for PTGR2
Browse R&D Systems for Antibodies
Antibodies from Abcam (ZADH1), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (ZADH1)
Novus Biologicals Antibodies for PTGR2

Assays for PTGR2: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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3 InterPro domains/families:
 IPR013149 ADH_Zn-bd
 IPR016040 NAD(P)-bd_dom
 IPR002085 ADH_SF_Zn


   GeneDecks  PTGR2 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q8N8N7

ProtoNet protein and cluster: Q8N8N7

UniProtKB/Swiss-Prot: PTGR2_HUMAN, Q8N8N7
Similarity: Belongs to the NADP-dependent oxidoreductase L4BD family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (PTGR2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (ZADH1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_152444

              Applied Biosystems Silencer® siRNAs for PTGR2

              Sigma-Aldrich siRNA and siRNA Panels for PTGR2  
                     Sigma-Aldrich shRNA for PTGR2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for PTGR2
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_152444
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_152444
                                 untagged cDNA clone in CMV expression vector: NM_152444 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_152444

UniProtKB/Swiss-Prot: PTGR2_HUMAN, Q8N8N7
Function: Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2,
15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest activity towards 15-keto-PGE2.
Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation
(By similarity)
Catalytic activity: 11-alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)(+) =
(5Z)-(13E)-11-alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H
Enzyme Number (IUBMB): EC 1.3.1.48 

4 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005488 binding IEA--
GO:0008270NOT zinc ion binding NAS--
GO:0016491 oxidoreductase activity NAS--
GO:0047522 15-oxoprostaglandin 13-oxidase activity IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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1 Sigma-Aldrich "Your Favorite Gene" Pathway for  PTGR2  (Your Favorite Gene powered by Ingenuity) 
 Inositol Metabolism

   GeneDecks  PTGR2 for the pathways selected above  
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  --

1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0055114 oxidation reduction IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for PTGR2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (PTGR2)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (ZADH1)
              OriGene 29mer shRNA kit in GFP-retroviral vector: NM_152444

              Sigma-Aldrich siRNA and siRNA Panels for PTGR2  
                     Sigma-Aldrich shRNA for PTGR2  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_152444  

REFSEQ mRNAs for PTGR2 gene (3 alternative transcripts): 

NM_001146154.1   NM_001146155.1   NM_152444.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_152444  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_152444
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_152444
                                 untagged cDNA clone in CMV expression vector: NM_152444 

Additional cDNA sequence: 

AK096410.1 AK309188.1 AK311280.1 AY346133.1 AY424308.1 BC059364.1 CR611156.1 

8 DOTS entries:

DT.97776487  DT.439661  DT.100680942  DT.120749920  DT.308359  DT.100669867  DT.101955267  DT.97818708 

24/427 AceView cDNA sequences (see all 427 ):

AL519085 AI367193 BC050683 CD367539 BM783687 BQ007801 BQ225836 BP377743 
BQ439827 CK903945 BQ018747 AU130416 BM905094 AA776314 AL043557 AA773474 
BF062869 BI916809 AI434688 AA601558 BG319454 AI185467 CR611156 Z41738 

Unigene Cluster for PTGR2:

Prostaglandin reductase 2
Hs.632344  [show with all ESTs]
Unigene Representative Sequence: AK096410


GeneLoc Exon Structure

5 Alternative Splicing Database (ASD) splice patterns (SP) for PTGR2

ExUns: 1a · 1b ^ 2a · 2b · 2c ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b ^ 8 ^ 9 ^ 10 ^ 11 ^ 12
SP1:                                                  -                                             
SP2:                          -                       -                                             
SP3:              -     -     -                       -                                             
SP4:                          -                                                                     
SP5:                          -                       -     -     -     -                           

About this scheme

ECgene alternative splicing isoforms for PTGR2

2 Ensembl transcripts including schematic representations:
ENST00000406080  ENST00000267568  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

PTGR2 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for PTGR2

1 / 2

3 probe-sets matching PTGR2 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
47626_at2 U95-B 1 1.00 1.00 1.00 1.00 -- -- -- -- --

230774_at2 U133-B 1 1.00 1.00 -- -- -- -- -- -- --

230774_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  PTGR2 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: AATGTTCAGG

SOURCE GeneReport for Unigene cluster: Hs.632344

UniProtKB/Swiss-Prot: PTGR2_HUMAN, Q8N8N7
Tissue specificity: Widely expressed

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for PTGR2 gene from 5/13 species (see all 13 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
PTGR21   -- prostaglandin reductase 2 92.21(n)
91.74(a)
480383  XM_849592.1  XP_854685.1 
chimpanzee
(Pan troglodytes)
PTGR21   -- prostaglandin reductase 2 100(n)
100(a)
740765  XM_001154481.1  XP_001154481.1 
cow
(Bos taurus)
PTGR21   -- prostaglandin reductase 2 91.45(n)
91.17(a)
506263  NM_001075249.1  NP_001068717.1 
rat
(Rattus norvegicus)
Ptgr21   -- prostaglandin reductase 2 84.27(n)
84.51(a)
299194  NM_001015009.1  NP_001015009.1 
mouse
(Mus musculus)
Ptgr21, 5 125
prostaglandin reductase 21, 5 85.09(n)1
86.61(a)1
772191  NM_029880.21  NP_084156.21 
 AI8387635  AK0075225  (see all 20)
About this table        Species with no ortholog for PTGR2

ENSEMBL Gene Tree for PTGR2
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for PTGR2 gene
ZADH22  PTGR12  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/249 NCBI SNPs in PTGR2 are shown (see all 249 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 56)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 14 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs22700741,2
A,C,F,H,O73388398(-) TGACCG/CTGTGG 1 -- ng51 tfbs38Minor allele frequency- C:0.44EA EU WA NA 1854
rs80202671,2
A,C,F,H73388467(+) TGAGGG/AAGAAG 1 -- ut51 ese3 trp36Minor allele frequency- A:0.06EU EA WA NA 590
rs80206301,2
A,C,F,H73388426(+) CCCGCG/AACTGT 1 -- ut5111Minor allele frequency- A:0.27EU EA WA NA 968
rs22700731,2
A,C,F,H73388507(-) AGCTCC/AGGGAG 1 -- ut51 ese311Minor allele frequency- A:0.48EU EA WA NA 902
rs21591791,2
A,C,F,H73386601(-) GTAAAG/AGAGCA 1 -- ng5113Minor allele frequency- A:0.49NS EU EA WA 942
rs80205231,2
A,C,F,H73388539(+) GCCCGG/CAACAC 1 -- ut51 ese37Minor allele frequency- C:0.14EU EA WA NA 554
rs80200911,2
A,F,H73388300(+) GCGTAC/GTGCGG 1 -- ng514Minor allele frequency- G:0.06EU EA WA 420
rs171826561,2
C,F,H73421066(+) TGATTC/TTTAAA 1 -- ut31 ese37Minor allele frequency- T:0.09NA EU EA WA 562
rs170927371,2
C,F,H73386753(+) AACTAC/TGCATT 1 -- ng5112Minor allele frequency- T:0.21NA EU EA WA 1024
rs157991,2
A,C,F73420862(-) TCAAGC/TGTTCT 1 -- ut314Minor allele frequency- T:0.47MN NA 326
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HapMap Linkage Disequilibrium images for PTGR2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 608642

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10 PubMed articles for PTGR2 gene:
(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 145482 HGNC: 20149 AceView: ZNF410andZADH1 Ensembl:ENSG00000140043 euGenes: HUgn145482
ECgene: PTGR2 H-InvDB: PTGR2
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for PTGR2:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



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 Predesigned and custom siRNAs for PTGR2 Antibodies for PTGR2
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 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
 Search Tocris compounds for PTGR2




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 Antibodies for PTGR2

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