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YSK4 Gene

protein-coding   GIFtS: 47

GC02M135438
YSK4 Sps1/Ste20-related kinase homolog (S. cerevisiae)
(Previous names: Yeast Sps1/Ste20-related kinase 4 (S. cerevisiae), yeast Sps1/Ste20-related kinase 4 (S. cerevisiae) )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
EC 2.7.11.1 3
FLJ23074 1, 2
RCK 2, 3
Descriptions
Regulated in COPD, protein kinase 3
YSK4 Sps1/Ste20-related kinase homolog (S. cerevisiae) 2
regulated in COPD kinase 2
yeast Sps1/Ste20-related kinase 4 2
yeast Sps1/Ste20-related kinase 4 (S. cerevisiae) 1
External Ids
HGNC: 262491
Entrez Gene: 801222
UniProtKB: Q56UN53
Ensembl: ENSG000001766017
Search outside databases for aliases for YSK4 gene

Previous GC identifer: GC02M135571

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

  --
(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the YSK4 gene  

Entrez Gene cytogenetic band: 2q21.3   Ensembl cytogenetic band:  2q21.3   HGNC cytogenetic band: 2q21.3

YSK4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02M135438:     (about GC identifiers)

Start:
135,438,743 bp from pter
End:
135,498,718 bp from pter
Size:
59,976 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000002.10  NT_022135.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: YSK4_HUMAN, Q56UN5 (See protein sequence)
Recommended Name: SPS1/STE20-related protein kinase YSK4  
Size: 1328 amino acids; 150537 Da
Secondary accessions: B2RP57 Q56UN1 Q56UN2 Q56UN3 Q56UN4 Q8N4E9 Q9H5T2
Alternative splicing: 6 isoforms:  Q56UN5-1   Q56UN5-2   Q56UN5-3   Q56UN5-4   Q56UN5-5   Q56UN5-6   

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (2 alternative transcripts): 
    NP_001018056.1  NP_079328.3  


    ENSEMBL proteins: 
    ENSP00000365005 ENSP00000351140 ENSP00000365004 ENSP00000376650 ENSP00000376649 ENSP00000321160 
    ENSP00000376647 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (Sps1/Ste20 related kinase 4)
    Human Recombinant Proteins from Abnova (YSK4)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 


    Antibodies for YSK4: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for YSK4
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (Sps1/Ste20 related kinase 4), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (YSK4)
    Novus Biologicals Antibody for YSK4

    Assays for YSK4: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    5/6 InterPro domains/families (see all 6 ):
     IPR017441 Protein_kinase_ATP_BS
     IPR002290 Ser_thr_pkinase
     IPR008271 Ser_thr_pkin_AS
     IPR000719 Prot_kinase_core
     IPR017442 Se/Thr_pkinase-rel


       GeneDecks  YSK4 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q56UN5

    ProtoNet protein and cluster: Q56UN5

    UniProtKB/Swiss-Prot: YSK4_HUMAN, Q56UN5
    Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20
    subfamily
    Similarity: Contains 1 protein kinase domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (YSK4)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (YSK4)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_025052

                  Applied Biosystems Silencer® siRNAs for YSK4

                  Sigma-Aldrich siRNA and siRNA Panels for YSK4  
                         Sigma-Aldrich shRNA Panels and shRNA for YSK4  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for YSK4
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001018046
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001018046
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001018046 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_025052

    UniProtKB/Swiss-Prot: YSK4_HUMAN, Q56UN5
    Catalytic activity: ATP + a protein = ADP + a phosphoprotein
    Enzyme Number (IUBMB): EC 2.7.11.1 

    4 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0004674 protein serine/threonine kinase activity IEA--
    GO:0005524 ATP binding IEA--
    GO:0016740 transferase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


      --

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006468 protein amino acid phosphorylation IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for YSK4

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (YSK4)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (YSK4)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_025052

                  Sigma-Aldrich siRNA and siRNA Panels for YSK4  
                         Sigma-Aldrich shRNA Panels and shRNA for YSK4  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_001018046  NM_025052  

    REFSEQ mRNAs for YSK4 gene (2 alternative transcripts): 

    NM_001018046.1   NM_025052.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_001018046  NM_025052  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001018046
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001018046
                                     untagged cDNA clones in CMV expression vector (see all 2): NM_001018046 

    Additional cDNA sequence: 

    AK122935.1 AK131216.1 AK225579.1 AK309454.1 AY574901.1 AY574902.1 AY574903.1 AY574904.1 
    AY574905.1 BC034417.1 BC137276.1 BC137277.1 BC144555.1 

    9 DOTS entries:

    DT.91917990  DT.403574  DT.121021305  DT.102825058  DT.121021292  DT.99996939  DT.97790639  DT.121021293 
    DT.121021303 

    24/25 AceView cDNA sequences (see all 25 ):

    BM969829 BQ045211 BC034417 AK131216 CA311871 AK122935 CD632388 CD632400 
    N57475 AK097522 CD632386 NM_025052 AK026727 BI832332 CD632385 CD632396 
    CD632398 CD632394 CD632402 BX090530 CD632392 N47620 CD632390 BU676900 

    highest scoring ESTs for YSK4:

    AY574901 AY574902 AY574904 AY574905 BC034417 BM969829 BQ045211 N57475 NM_025052 AI683447 

    Unigene Cluster for YSK4:

    YSK4 Sps1/Ste20-related kinase homolog (S. cerevisiae)
    Hs.659395  [show with all ESTs]
    Unigene Representative Sequence: AK122935


    GeneLoc Exon Structure

    5/6 Alternative Splicing Database (ASD) splice patterns (SP) for YSK4 (see all 6 )

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9a · 9b ^ 10a · 10b ^ 11a · 11b · 11c · 11d · 11e
    SP1:                                                              -                                       
    SP2:        -                                                     -     -                                 
    SP3:        -                                   -           -     -     -     -                           
    SP4:        -                                   -                 -     -                                 
    SP5:        -                 -     -                             -     -                                 

    About this scheme

    ECgene alternative splicing isoforms for YSK4

    7 Ensembl transcripts including schematic representations:
    ENST00000375845  ENST00000358371  ENST00000375844  ENST00000392918  ENST00000392917  ENST00000315513  
    ENST00000392915  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    YSK4 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for YSK4

    1 / 2 / 3

    4 probe-sets matching YSK4 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    55473_at2, 3 U95-C 1 1.00 1.00 1.00 1.00 N57475 0.60 1.00 0.82 1

    221180_at2, 3 U133-A 1 1.00 1.00 -- -- NM_025052 0.60 1.00 0.82 1

    221180_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    1555804_a_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TGCCTGACCA

    SOURCE GeneReport for Unigene cluster: Hs.659395
    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for YSK4 gene from 5/7 species (see all 7 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    YSK41   -- YSK4 Sps1/Ste20-related kinase homolog (S. cerevisiae) 84.63(n)
    74.73(a)
    483897  XM_541017.2  XP_541017.2 
    chimpanzee
    (Pan troglodytes)
    YSK41   -- YSK4 Sps1/Ste20-related kinase homolog (S. cerevisiae) 99.02(n)
    98.19(a)
    470547  XM_525928.2  XP_525928.2 
    cow
    (Bos taurus)
    YSK41   -- YSK4 Sps1/Ste20-related kinase homolog (S. cerevisiae) 83.79(n)
    73.23(a)
    538897  XM_583510.2  XP_583510.2 
    mouse
    (Mus musculus)
    Ysk41, 5 15
    Yeast Sps1/Ste20-related kinase 4 (S. cerevisiae)1, 5 73.76(n)1
    60.75(a)1
    226251  XM_001477564.11  XP_001477614.11 
     U499515 
    chicken
    (Gallus gallus)
    YSK41   -- yeast Sps1/Ste20-related kinase 4 (S. cerevisiae) 56.86(n)
    44.64(a)
    429048  XM_426605.2  XP_426605.2 
    About this table        Species with no ortholog for YSK4

    ENSEMBL Gene Tree for YSK4
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for YSK4 gene
    MAP3K32  MAP3K22  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/315 NCBI SNPs in YSK4 are shown (see all 315 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 91)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 2 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs15921,2
    A,C,F,H,O135438613(+) AAACAC/ATAGAG 2 -- ng5113Minor allele frequency- A:0.41EA MN NA EU WA 2588
    rs109285291,2
    A,C,F,H135498926(+) AATGTG/ATGAAT 2 -- ng313Minor allele frequency- A:0.17EU EA 296
    rs49542131,2
    A,C,F,H135500697(+) AAAGTG/TCATTG 2 -- ng314Minor allele frequency- T:0.35EU EA WA 418
    rs11125421,2
    C,F,H,O135460886(-) TGCGAG/CAGACT 2 Q/E mis1 int17Minor allele frequency- C:0.20EU EA WA NA 556
    rs39053171,2
    C,F,H135460477(-) TGAAGA/GAGTTT 2 E/G mis1 int17Minor allele frequency- G:0.16EU EA WA NA 560
    rs37690221,2
    C,F,H135438568(-) GTCAAT/CTTTAA 2 -- ng518Minor allele frequency- C:0.20EA NA EU WA 1300
    --
    rs75586781,2
    A,C,F135500644(+) GAAACT/CGCAAT 2 -- ng313Minor allele frequency- C:0.01NA 142
    rs168312351,2
    C,F,H135461599(+) GTACAA/GTACAC 2 T/I mis1 int1 ese316Minor allele frequency- N:0.00NA EU EA WA 1474
    --
    rs75649811,2
    A,C135500342(+) TTCCAG/CTattc 2 -- ng31 tfbs33Minor allele frequency- C:0.01NA 142
    --
    rs168312151,2
    F,H135438798(+) TTTTCA/GGTGGG 2 -- ut317Minor allele frequency- G:0.03NA EU EA WA 562
    About this table

    HapMap Linkage Disequilibrium images for YSK4 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

      --

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    6 PubMed articles for YSK4 gene:
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 80122 HGNC: 26249 AceView: FLJ23074 Ensembl:ENSG00000176601 euGenes: HUgn80122
    ECgene: YSK4 H-InvDB: YSK4
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for YSK4:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
      Invitrogen Antibodies  Invitrogen Assays
      Invitrogen Clones  Invitrogen Q-PCR Products
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     Predesigned and custom siRNAs for YSK4 Antibodies for YSK4
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for YSK4 Browse small molecules at Sigma-Aldrich
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     Antibodies for YSK4

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