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XRCC2 Gene

protein-coding   GIFtS: 62

GC07M151974
X-ray repair complementing defective repair in Chinese hamster cells 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
DKFZp781P0919 2
RAD51-like 2
Descriptions
DNA repair protein XRCC2 2
X-ray repair complementing defective repair in Chinese
hamster cells 2 2
X-ray repair cross complementing protein 2 2
X-ray repair cross-complementing protein 2 3
X-ray repair, complementing defective, repair in Chinese
hamster 2
External Ids
HGNC: 128291
Entrez Gene: 75162
UniProtKB: O435433
Ensembl: ENSG000001965847
Search outside databases for aliases for XRCC2 gene

Previous GC identifers: GC07M150590 GC07M151658 GC07M151735 GC07M151781

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for XRCC2:
This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous
recombination to maintain chromosome stability and repair DNA damage. This gene is involved in the
repair of DNA double-strand breaks by homologous recombination and it functionally complements
Chinese hamster irs1, a repair-deficient mutant that exhibits hypersensitivity to a number of
different DNA-damaging agents. [provided by RefSeq]

UniProtKB/Swiss-Prot: XRCC2_HUMAN, O43543
Function: Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA,
thought to repair chromosomal fragmentation, translocations and deletions. The BCDX2 complex binds
single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA

Gene Wiki entry for XRCC2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the XRCC2 gene  

Entrez Gene cytogenetic band: 7q36.1   Ensembl cytogenetic band:  7q36.1   HGNC cytogenetic band: 7q36

XRCC2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 7         GeneLoc Exon Structure

GeneLoc location for GC07M151974:     (about GC identifiers)

Start:
151,974,516 bp from pter
End:
152,004,183 bp from pter
Size:
29,668 bases
Orientation:
minus strand

1 alternative location:
Chr7-,CRA_TCAG 151,670,667-151,700,330     
RefSeq DNA sequence:
NC_000007.12  NT_007914.14  NT_079596.2  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: XRCC2_HUMAN, O43543 (See protein sequence)
Recommended Name: DNA repair protein XRCC2  
Size: 280 amino acids; 31956 Da
Subunit: Interacts with RAD51D. Part of a BCDX2 complex consisting of RAD51B, RAD51C, RAD51D and
XRCC2. Part of a complex consisting of RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3. In the absence of
DNA, XRCC2-RAD51D formed a multimeric ring structure. In the presence of single-stranded DNA,
XRCC2-RAD51D formed a filamentous structure
Subcellular location: Nucleus

Post-translational modifications:

  • Phosphorylated upon DNA damage, probably by ATM or ATR1
  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_005422.1  

    ENSEMBL proteins: 
    ENSP00000352271 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (XRCC2)
    Human Recombinant Proteins from Abnova (XRCC2)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634 nucleus IEA--
    About this table

    Antibodies for XRCC2: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (XRCC2), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (XRCC2)
    Novus Biologicals Antibodies for XRCC2

    Assays for XRCC2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR001553 RecA_bac


       GeneDecks  XRCC2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry O43543

    ProtoNet protein and cluster: O43543

    UniProtKB/Swiss-Prot: XRCC2_HUMAN, O43543
    Similarity: Belongs to the recA family. RAD51 subfamily

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (XRCC2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (XRCC2)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005431

                  Applied Biosystems Silencer® siRNAs for XRCC2

                  Sigma-Aldrich siRNA and siRNA Panels for XRCC2  
                         Sigma-Aldrich shRNA Panels and shRNA for XRCC2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for XRCC2
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_005431
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_005431
                                     untagged cDNA clone in CMV expression vector: NM_005431 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_005431

    UniProtKB/Swiss-Prot: XRCC2_HUMAN, O43543
    Function: Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA,
    thought to repair chromosomal fragmentation, translocations and deletions. The BCDX2 complex binds
    single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA

    Genatlas biochemistry entry for XRCC2:
    repair X-ray defect complementing defect in CHO cells,2,promoting the repair of DNA double strand
    breaks by homologous recombination

    12 MGI mutant phenotypes (inferred from 3 alleles(MGI details for Xrcc2):

    cellularcraniofacialembryogenesisgrowth/sizelethality-prenatal/perinatal
    life span-post-weaning/aginglimbs/digits/tailnervous systemrespiratory systemskeleton
    skin/coat/nailstumorigenesis

    3 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003677 DNA binding IEA--
    GO:0005524 ATP binding IEA--
    GO:0008094 DNA-dependent ATPase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Kegg Pathway  (Kegg details for XRCC2):
     hsa03440 Homologous recombination

       GeneDecks  XRCC2 for the pathways selected above  
    About GeneDecksing
     Gene Network CentralTM Interacting Genes and Proteins Network for  XRCC2 


    5/19 Interacting proteins for XRCC2 (ENSP000003522713 O435432) via UniProtKB, MINT, and/or STRING (see all 19 )
    InteractantInteraction Details
    GeneCardExternal ID(s)
    RAD51L3O757712STRING (score=.999) MINT-2730528 MINT-18212
    XRCC3ENSP000003433923STRING (score=.993)
    RAD51CENSP000003367013STRING (score=.928)
    IRS1ENSP000003048953STRING (score=.836)
    RAD51L1ENSP000003405603STRING (score=.754)
    About this table

    5/10 Gene Ontology (GO) biological process terms (links to tree view) (see all 10 ):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000724 double-strand break repair via homologous recombination IEA--
    GO:0001701 in utero embryonic development IEA--
    GO:0006281 DNA repair TAS9628903
    GO:0006310 DNA recombination IEA--
    GO:0006974 response to DNA damage stimulus IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for XRCC2

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (XRCC2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (XRCC2)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_005431

                  Sigma-Aldrich siRNA and siRNA Panels for XRCC2  
                         Sigma-Aldrich shRNA Panels and shRNA for XRCC2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_005431  

    REFSEQ mRNAs for XRCC2 gene: 

    NM_005431.1   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_005431  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_005431
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_005431
                                     untagged cDNA clone in CMV expression vector: NM_005431 

    Additional cDNA sequence: 

    AF035587.1 AK313607.1 BC042137.1 CR749256.1 Y08837.1 

    2 DOTS entries:

    DT.91861682  DT.91749840 

    20 AceView cDNA sequences:

    NM_005431 BU182381 BC042137 AF035587 BQ068576 BV208285 AA978101 BE780820 
    BX283699 AI680402 Y08837 BX104116 CR749256 AI346114 BF744461 AA677337 
    BQ430896 AW080499 BG776777 BG494177 

    highest scoring ESTs for XRCC2:

    Y08837 AF035587 BC042137 BQ068576 BX283699 CN290945 CN290947 NM_005431 AA255634 AI346114 

    Unigene Cluster for XRCC2:

    X-ray repair complementing defective repair in Chinese hamster cells 2
    Hs.647093  [show with all ESTs]
    Unigene Representative Sequence: CR749256


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000359321  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    XRCC2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for XRCC2

    1 / 2 / 3

    4 probe-sets matching XRCC2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    1300_at2, 3 U95-A 1 1.00 1.00 0.76 0.85 Y08837 1.00 1.00 1.00 1
    83115_at*2, 3 U95-D 1 -- -- 0.98 1.18 AI346114 0.40 1.00 0.76 1

    207598_x_at2, 3 U133-A 9 1.00 0.77 -- -- NM_005431 0.60 1.00 0.82 1

    207598_x_at2 U133Plus2 9 1.00 0.77 -- -- -- -- -- -- --
    GeneDecks  XRCC2 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: --

    SOURCE GeneReport for Unigene cluster: Hs.647093

    Expression variation in blood from EXPOLDB for XRCC2

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for XRCC2 gene from 5/7 species (see all 7 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    XRCC21   -- X-ray repair complementing defective repair in Chinese more 86.14(n)
    82.4(a)
    475548  XM_532771.2  XP_532771.2 
    chimpanzee
    (Pan troglodytes)
    XRCC21   -- X-ray repair complementing defective repair in Chinese more 99.64(n)
    99.29(a)
    738997  XM_001140134.1  XP_001140134.1 
    cow
    (Bos taurus)
    XRCC21   -- X-ray repair complementing defective repair in Chinese more 84.95(n)
    84.23(a)
    789410  XM_001256172.1  XP_001256173.1 
    rat
    (Rattus norvegicus)
    Xrcc21   -- X-ray repair complementing defective repair in Chinese more 79.62(n)
    79.86(a)
    499966  XM_575317.2  XP_575317.1 
    mouse
    (Mus musculus)
    Xrcc21, 5 55
    X-ray repair complementing defective repair in Chinese more1, 5 79.14(n)1
    78.42(a)1
    574341  NM_020570.21  NP_065595.21 
     AK0201925  AV3381385  (see all 7)
    About this table        Species with no ortholog for XRCC2

    ENSEMBL Gene Tree for XRCC2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

      --
    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/330 NCBI SNPs in XRCC2 are shown (see all 330 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 91)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 7 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs20406391,2
    A,C,F,H152006121(-) GTTGCG/ACTCAC 1 -- ng315Minor allele frequency- A:0.39MN EU EA WA 598
    rs64642651,2
    A,C,F,H152005867(+) ATCTTC/TATTAC 1 -- ng314Minor allele frequency- T:0.07EU EA WA 418
    rs69678261,2
    A,C,F,H152006113(+) CTGTTC/TGTGTG 1 -- ng312Minor allele frequency- T:0.13EU WA 240
    rs32183731,2
    A,C,F,H152005096(-) ttcagG/Tacgta 1 -- ng3119Minor allele frequency- T:0.19NS EU EA WA 2100
    rs32185361,2
    C,F,H151976940(-) CTATCG/ACCTGG 1 H/R mis1 ese321Minor allele frequency- A:0.03NS MN CSAM EA EU WA 2266
    --
    rs32183741,2
    C,F,H152005067(-) acttgC/Gaacca 1 -- ng315Minor allele frequency- G:0.37NS EU EA WA 590
    --
    rs32185391,2
    C,F,H151976659(-) GTTGAT/CATACA 1 -- ut3112Minor allele frequency- C:0.05NS MN EU EA WA 1434
    --
    rs32183711,2
    A,C,F152005326(-) ACACGA/C/GTTCAG 1 -- ng311NS 176
    --
    rs32185441,2
    A,C,F151976161(-) AGGCCC/TGTGCC 1 -- ut31 ese31Minor allele frequency- T:0.36NS 176
    rs49870901,2
    C,F,H151988794(-) TCCTTG/TCCCGA 1 A/S mis1 ese314Minor allele frequency- T:0.00MN CSAM EA EU WA 1426
    About this table

    HapMap Linkage Disequilibrium images for XRCC2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 600375

    2 Novoseek disease relationships for XRCC2 gene

    Disease   Score   Articles   PubMed IDs for Articles with Shared Sentences (# sentences)
    cancer 27.84 4 15386379 (1), 17141189 (1), 19116388 (1)
    breast cancer 17.59 9 18188695 (3), 12023982 (2), 16702393 (1), 16485136 (1) (see all 5)
    About this table

    Human Gene Mutation Database: XRCC2
    Genetic Association Database: XRCC2
    Human Genome Epidemiology Navigator: XRCC2 (50 documents)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/105 PubMed articles for XRCC2 gene (see all 105 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 7516 HGNC: 12829 AceView: XRCC2 Ensembl:ENSG00000196584 euGenes: HUgn7516
    ECgene: XRCC2 H-InvDB: XRCC2
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

    NameDescription
    ATLAS Chromosomes in Cancer entry for XRCC2 Genetics and Cytogenetics in Oncology and Haematology
    NIEHS-SNPshttp://egp.gs.washington.edu/data/xrcc2/
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for XRCC2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Antibodies for XRCC2

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