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XPNPEP2 Gene

protein-coding   GIFtS: 60

GC0XP128700
X-prolyl aminopeptidase (aminopeptidase P) 2, membrane-bound
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases
EC 3.4.11.9 3
OTTHUMP00000023987 2
mAmP 3
Descriptions
Aminoacylproline aminopeptidase 2, 3
Membrane-bound APP 3
Membrane-bound AmP 3
Membrane-bound aminopeptidase P 3
X-Pro aminopeptidase 2 3
X-prolyl aminopeptidase (aminopeptidase P) 2,
membrane-bound 2
X-prolyl aminopeptidase 2, membrane-bound 2
External Ids
HGNC: 128231
Entrez Gene: 75122
UniProtKB: O438953
Ensembl: ENSG000001221217
Search outside databases for aliases for XPNPEP2 gene

Previous GC identifers: GC0XP123518 GC0XP125718 GC0XP126817 GC0XP127578 GC0XP128598

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for XPNPEP2:
Aminopeptidase P is a hydrolase specific for N-terminal imido bonds, which are common to several
collagen degradation products, neuropeptides, vasoactive peptides, and cytokines. Structurally,
the enzyme is a member of the 'pita bread fold' family and occurs in mammalian tissues
in both soluble and GPI-anchored membrane-bound forms. A membrane-bound and soluble form of this
enzyme have been identified as products of two separate genes. [provided by RefSeq]

UniProtKB/Swiss-Prot: XPP2_HUMAN, O43895
Function: A metalloprotease that may play a role in the inflammatory process and other reactions
produced in response to injury or infection. May also play a role in the metabolism of the
vasodilator bradykinin

Gene Wiki entry for XPNPEP2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the XPNPEP2 gene  

Entrez Gene cytogenetic band: Xq25   Ensembl cytogenetic band:  Xq25   HGNC cytogenetic band: Xq25

XPNPEP2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome X         GeneLoc Exon Structure

GeneLoc location for GC0XP128700:     (about GC identifiers)

Start:
128,700,627 bp from pter
End:
128,731,206 bp from pter
Size:
30,580 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000023.9  NT_011786.15  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: XPP2_HUMAN, O43895 (See protein sequence)
Recommended Name: Xaa-Pro aminopeptidase 2 precursor  
Size: 674 amino acids; 75625 Da
Cofactor: Binds 2 manganese ions per subunit (By similarity)
Subunit: Homotrimer
Subcellular location: Cell membrane; Lipid-anchor, GPI-anchor
Secondary accessions: A0AV16 O75994

Post-translational modifications:

  • Heavily glycosylated1
  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_003390.4  

    ENSEMBL proteins: 
    ENSP00000360146 ENSP00000360147 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    R&D Systems Recombinant & Natural Proteins for XPNPEP2 (Aminopeptidase P2/XPNPEP2)
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse Abcam for Recombinant Proteins
    Browse Abnova for Human Recombinant Proteins
                  OriGene Purified Recombinant Human Protein: XPNPEP2 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005886 plasma membrane IEA--
    GO:0031225 anchored to membrane IEA--
    About this table

    Antibodies for XPNPEP2: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for XPNPEP2
    R&D Systems Antibodies for XPNPEP2 (Aminopeptidase P2/XPNPEP2)
    Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
    Browse Abnova for Monoclonal and Polyclonal Antibodies
    Search Novus for antibodies for XPNPEP2

    Assays for XPNPEP2: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    3 InterPro domains/families:
     IPR000994 Pept_M24_structural-domain
     IPR001131 Peptidase_M24B_aminopep-P_CS
     IPR000587 Creatinase


       GeneDecks  XPNPEP2 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry O43895

    ProtoNet protein and cluster: O43895

    UniProtKB/Swiss-Prot: XPP2_HUMAN, O43895
    Similarity: Belongs to the peptidase M24B family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (XPNPEP2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (XPNPEP2)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003399

                  Applied Biosystems Silencer® siRNAs for XPNPEP2

                  Sigma-Aldrich siRNA and siRNA Panels for XPNPEP2  
                         Sigma-Aldrich shRNA for XPNPEP2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_003399
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_003399
                                     untagged cDNA clone in CMV expression vector: NM_003399 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_003399

    UniProtKB/Swiss-Prot: XPP2_HUMAN, O43895
    Function: A metalloprotease that may play a role in the inflammatory process and other reactions
    produced in response to injury or infection. May also play a role in the metabolism of the
    vasodilator bradykinin
    Catalytic activity: Release of any N-terminal amino acid, including proline, that is linked to
    proline, even from a dipeptide or tripeptide
    Enzyme Number (IUBMB): EC 3.4.11.9 

    Genatlas biochemistry entry for XPNPEP2:
    aminopeptidase P,75.49kDa,hydrolyzing N terminal imido bonds

    5 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004177 aminopeptidase activity TAS9375790
    GO:0008233 peptidase activity IEA--
    GO:0008235 metalloexopeptidase activity IEA--
    GO:0030145 manganese ion binding IEA--
    GO:0046872 metal ion binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    4 Interacting proteins for XPNPEP2 (ENSP000003601473) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    APPENSP000002849813STRING (score=.894)
    POF1BENSP000003622383STRING (score=.746)
    ACEENSP000002908663STRING (score=.722)
    ACE2ENSP000002525193STRING (score=.722)
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006508 proteolysis IEA--
    GO:0009987 cellular process IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Enzo Life Sciences drugs & compounds for XPNPEP2
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for XPNPEP2

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (XPNPEP2)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (XPNPEP2)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_003399

                  Sigma-Aldrich siRNA and siRNA Panels for XPNPEP2  
                         Sigma-Aldrich shRNA for XPNPEP2  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_003399  

    REFSEQ mRNAs for XPNPEP2 gene: 

    NM_003399.5   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_003399  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_003399
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_003399
                                     untagged cDNA clone in CMV expression vector: NM_003399 

    Additional cDNA sequence: 

    AF086417.1 AK300954.1 BC126174.1 BC143901.1 U90724.2 

    3 DOTS entries:

    DT.99986069  DT.410965  DT.100760683 

    24/34 AceView cDNA sequences (see all 34 ):

    BU677870 AW275469 AW275371 AI358577 AF086417 BQ224207 CD518663 BX386512 
    BX386511 AI912887 AI473757 AW204294 AW025328 CD617071 AW601930 AA778144 
    AA040482 AI269228 W67831 CF529129 T24561 AA341827 AW849140 AA917912 

    highest scoring ESTs for XPNPEP2:

    AA040482 AA341827 AA778144 AA917912 AF086417 AI269228 AI358577 AW025328 AW204294 AW275469 

    Unigene Cluster for XPNPEP2:

    X-prolyl aminopeptidase (aminopeptidase P) 2, membrane-bound
    Hs.170499  [show with all ESTs]
    Unigene Representative Sequence: U90724


    GeneLoc Exon Structure

    2 Ensembl transcripts including schematic representations:
    ENST00000371105  ENST00000371106  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    XPNPEP2 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for XPNPEP2

    1 / 2 / 3

    5 probe-sets matching XPNPEP2 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    40037_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 AL023653 0.20 0.50 0.38 1

    216910_at2 U133-A 1 1.00 1.00 -- -- -- -- -- -- --

    206484_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_003399 0.60 1.00 0.82 1

    216910_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    206484_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  XPNPEP2 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: CAGAGTCCCT

    SOURCE GeneReport for Unigene cluster: Hs.170499

    Expression variation in blood from EXPOLDB for XPNPEP2

    UniProtKB/Swiss-Prot: XPP2_HUMAN, O43895
    Tissue specificity: Expressed in kidney, lung, heart, placenta, liver, small intestine and colon.
    No expression in brain, skeletal muscle, pancreas, spleen, thymus, prostate, testis and ovary

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for XPNPEP2 gene from 5/10 species (see all 10 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    XPNPEP21   -- X-prolyl aminopeptidase (aminopeptidase P) 2, membrane-bound 85.98(n)
    83.54(a)
    492124  XM_549245.2  XP_549245.2 
    chimpanzee
    (Pan troglodytes)
    XPNPEP21   -- X-prolyl aminopeptidase (aminopeptidase P) 2, membrane-bound 96.74(n)
    95.1(a)
    465852  XM_521256.2  XP_521256.2 
    cow
    (Bos taurus)
    XPNPEP21   -- X-prolyl aminopeptidase (aminopeptidase P) 2, membrane-bound 86.77(n)
    86.26(a)
    504822  XM_580998.3  XP_580998.3 
    rat
    (Rattus norvegicus)
    Xpnpep21   -- X-prolyl aminopeptidase (aminopeptidase P) 2, membrane-bound 82.37(n)
    81.58(a)
    117522  NM_057155.2  NP_476496.1 
    mouse
    (Mus musculus)
    Xpnpep21, 5 X5
    X-prolyl aminopeptidase (aminopeptidase P) 2, membrane-bound1, 5 83.01(n)1
    81.43(a)1
    1707451  NM_133213.21  NP_573476.21 
     AF3672475  AF4281025  (see all 12)
    About this table        Species with no ortholog for XPNPEP2

    ENSEMBL Gene Tree for XPNPEP2
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for XPNPEP2 gene
    XPNPEP12  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/165 NCBI SNPs in XPNPEP2 are shown (see all 165 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 73)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr X posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs20500111,2
    A,C,F,H,O128699259(+) TGGATT/GGTTGA 1 -- ng518Minor allele frequency- G:0.29EU EA WA NA 780
    rs8595851,2
    A,C,F,H,O128730544(+) GTCCCG/AGGAGA 1 -- ut31 ese312Minor allele frequency- A:0.50NA EA EU WA 988
    rs66376561,2
    C,F,H128730576(+) TTGGGC/TATAGC 1 -- ut3112Minor allele frequency- T:0.18EU EA WA NS NA 666
    rs22354441,2
    C,F,H128700478(+) TGGAGG/AGAAGG 1 -- ng51 trp34Minor allele frequency- A:0.13EU EA WA 418
    --
    rs59326671,2
    A,C128699601(+) AAATCA/GCCTTC 1 -- ng51 tfbs30--------
    rs22811331,2
    F,H128731153(+) CCTGGG/TTCCAC 1 -- ut31 ese34Minor allele frequency- T:0.06EU EA WA 416
    rs120070471,2
    C128699589(+) TTCCTC/GTCTGG 1 -- ng51 tfbs30--------
    --
    rs173146651,2
    F128730305(+) CCAGGC/ACCCAG 1 -- ut31 ese33Minor allele frequency- A:0.16NA 114
    rs120127021,2
    C128730579(+) GGCATA/GGCCAG 1 -- ut310--------
    rs20922431,2
    H128699233(+) AAATGG/TCCTAT 1 -- ng513Minor allele frequency- T:0.00EA WA 298
    About this table

    HapMap Linkage Disequilibrium images for XPNPEP2 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 300145

    Human Gene Mutation Database: XPNPEP2
    Genetic Association Database: XPNPEP2
    Human Genome Epidemiology Navigator: XPNPEP2 (1 document)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/20 PubMed articles for XPNPEP2 gene (see all 20 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 7512 HGNC: 12823 AceView: XPNPEP2 Ensembl:ENSG00000122121 euGenes: HUgn7512
    ECgene: XPNPEP2 H-InvDB: XPNPEP2
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for XPNPEP2:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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     Predesigned and custom siRNAs for XPNPEP2 Antibodies for XPNPEP2
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for XPNPEP2 Browse small molecules at Sigma-Aldrich
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     Drugs for XPNPEP2
     Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
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