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XAGE3 Gene

protein-coding GIFtS: 40

GC0XM052908

X antigen family, member 3
(Previous names: placenta-specific 6; G antigen, family D, 4 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbols: PLAC6, GAGED4)

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
CT12.3 ^³
CT12.3a ^¹, ²
GAGED4 ^², ³
MGC71925 ^²
OTTHUMP00000023319 ^²
OTTHUMP00000023320 ^²
PLAC6 ^², ³, ⁵
XAGE-3 ^¹, ²
pp9012 ^¹, ²

Descriptions
Cancer/testis antigen 12.3 ^³
G antigen, family D, 4 ^²
Placenta-specific gene 6 protein ^³
Protein XAGE-3 ^³
X antigen family, member 3 ^²
XAGE-3 protein ^²
cancer/testis antigen family 12, member 3a ^²
placenta-specific 6 ^²
placenta-specific 6; G antigen, family D, 4 ^¹

External Ids
HGNC: 14618¹
Entrez Gene: 170626²
UniProtKB: Q8WTP9³
Ensembl: ENSG00000171402⁷

Search outside databases for aliases for XAGE3 gene

Previous GC identifer: GC0XM052775

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for XAGE3:

This gene is a member of the XAGE subfamily, which belongs to the GAGE family. The GAGE genes are

expressed in a variety of tumors and in some fetal and reproductive tissues. This gene is

expressed in placenta and fetal liver/spleen, and may function in inhibiting cancer cell growth.

The protein encoded by this gene shares a sequence similarity with other GAGE/PAGE proteins.

Because of the expression pattern and the sequence similarity, this protein also belongs to a

family of CT (cancer-testis) antigens. Alternative splicing of this gene generates 2 transcript

variants differing in the 5' UTR. [provided by RefSeq]

UniProtKB/Swiss-Prot: GAGD4_HUMAN, Q8WTP9

Function: Unknown

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: Xp11.22-p11.21 Ensembl cytogenetic band: Xp11.22 HGNC cytogenetic band: Xp11.22

XAGE3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome X GeneLoc Exon Structure

GeneLoc location for GC0XM052908: (about GC identifiers)

Start:	52,908,282 bp from pter
End:	52,913,844 bp from pter
Size:	5,563 bases
Orientation:	minus strand

RefSeq DNA sequence:

NC_000023.9 NT_011630.14

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: GAGD4_HUMAN, Q8WTP9 (See protein sequence)

Recommended Name: G antigen family D member 4

Size: 111 amino acids; 12302 Da

Secondary accessions: Q5JS82 Q8WYS9

REFSEQ proteins (2 alternative transcripts):

NP_570132.1 NP_573440.1

ENSEMBL proteins:

ENSP00000303061 ENSP00000364640

Human Recombinant Proteins

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	Browse Abcam for Recombinant Proteins
	Browse Abnova for Human Recombinant Proteins

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

Antibodies for XAGE3:

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	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
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	Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
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Assays for XAGE3:

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	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

Graphical View of Domain Structure for InterPro Entry Q8WTP9

ProtoNet protein and cluster: Q8WTP9

1 Blocks protein family: IPB008625 GAGE

UniProtKB/Swiss-Prot: GAGD4_HUMAN, Q8WTP9

Similarity: Belongs to the GAGE family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (XAGE3)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_133179

Applied Biosystems Silencer® siRNAs for XAGE3

Sigma-Aldrich siRNA for XAGE3

Sigma-Aldrich shRNA for XAGE3

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Invitrogen Clones for XAGE3
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_130776
Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_130776
untagged cDNA clones in CMV expression vector (see all 2): NM_130776

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_133179

UniProtKB/Swiss-Prot: GAGD4_HUMAN, Q8WTP9

Function: Unknown

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for XAGE3

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (XAGE3)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_133179

Sigma-Aldrich siRNA for XAGE3

Sigma-Aldrich shRNA for XAGE3

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_130776 NM_133179

REFSEQ mRNAs for XAGE3 gene (2 alternative transcripts):

NM_130776.1 NM_133179.2

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_130776 NM_133179

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_130776
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_130776
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_130776

Additional cDNA sequence:

AF318372.1 AJ318881.1 BC062680.1

3 DOTS entries:

DT.101970658 DT.97825141 DT.410605

24 AceView cDNA sequences:

AJ318881 N32547 NM_130776 N41436 NM_133179 AI189067 AF318372 BC062680

BG354572 N41892 C18417 D59091 BX283272 CF993800 AI742551 T84951

N41723 N40147 BG436468 CB992682 CB994958 CB961487 BG436305 BG435916

highest scoring ESTs for XAGE3:

N40147 AF318372 BG354572 BG436305 BG436468 D59091 AI189067 AI742551 AJ318881 BC062680

Unigene Cluster for XAGE3:

X antigen family, member 3

Hs.43879 [show with all ESTs]

Unigene Representative Sequence: CF993800

GeneLoc Exon Structure

2 Ensembl transcripts including schematic representations:

ENST00000346279 ENST00000375491

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

XAGE3 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for XAGE3

¹ / ² / ³

4 probe-sets matching XAGE3 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
53563_f_at²^{, 3}	U95-B	12	1.00	0.77	0.56	0.47	N32547	0.60	1.00	0.82	1
64667_at²^{, 3}	U95-C	13	0.94	0.79	1.00	1.00	N40147	1.00	1.00	1.00	1
236040_at²^{, 3}	U133-B	8	1.00	0.79	--	--	AI742551	0.60	1.00	0.82	1
236040_at²	U133Plus2	8	1.00	0.79	--	--	--	--	--	--	--

About this table

Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.43879

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for XAGE3 gene from 1 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
chimpanzee (Pan troglodytes)	XAGE3¹	--	X antigen family, member 3	99.1(n) 99.1(a)	741714 XM_001144042.1 XP_001144042.1

About this table Species with no ortholog for XAGE3

ENSEMBL Gene Tree for XAGE3

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for XAGE3 gene

GAGE2D² GAGE10² GAGE12H² ENSG00000169164² XAGE5² XAGE2B² GAGE12C² GAGE12E²

GAGE2E² GAGE1² GAGE2C² GAGE12F² GAGE13² PAGE1² XAGE2² GAGE12G²

GAGE2B² GAGE2A² GAGE12J²

Pseudogenes

1 related pseudogene

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/27 NCBI SNPs in XAGE3 are shown (see all 27 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 7)

AB	SNP ID	Valid	Chr X pos	Sequence	Recs	AA Chg	Type	More	Recs	Allele freq	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--				--
	rs2806851^1,2	A,C,F,H	52915478(+)	`CCTCTA/GAATGA`	1	--	ng3¹		8		EU EA WA	628
--	rs4615770^1,2	C,F,H	52915512(+)	`TGGAGG/TAGAGT`	1	--	ng3¹		4		EU EA WA	418
	rs5943751^1,2	A,F,H	52908309(+)	`TATTTC/TGATAT`	2	--	ut3¹		4		EU EA WA	416
--	rs2491154^1,2	A,C	52914320(-)	`GACATC/TATGAC`	2	--	ng3¹		0	--	--	--	--
--	rs2491155^1,2	A,C	52914303(-)	`CTGTGC/TTGCTG`	2	--	ng3¹ tfbs³		0	--	--	--	--
--	rs2807031^1,2	A,C	52913674(-)	`AGCATA/GGCCCG`	2	--	int¹ ng3¹		0	--	--	--	--
--	rs4143221^1,2	H	52912289(-)	`AGGAGC/Accggt`	2	T/P	mis¹ ese³		4		EU EA WA	418
--	rs73206487^1,2	--	52908058(+)	`CATATA/GTACAT`	2	--	ng5¹		0	--	--	--	--
--	rs41306369^1,2	--	52913102(+)	`TTAAGA/GCTCCT`	2	--	ng3¹ int¹		0	--	--	--	--
--	rs73206483^1,2	--	52907917(+)	`CTTTTC/TTTTTT`	2	--	ng5¹		0	--	--	--	--

About this table

HapMap Linkage Disequilibrium images for XAGE3 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 300740

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

6 PubMed articles for XAGE3 gene:

The DNA sequence of the human X chromosome. (PubMed id 15772651)^{3, 4} Ross M.T.... Bentley D.R. (2005)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
Large-scale cDNA transfection screening for genes related to cancer development and progression. (PubMed id 15498874)^{3, 4} Wan D....Gu J. (2004)
The XAGE family of cancer/testis-associated genes: alignment and expression profile in normal tissues, melanoma lesions and Ewing's sarcoma. (PubMed id 11992404)^{3, 4} Zendman A.J.W....van Muijen G.N.P. (2002)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)
Novel genes in the PAGE and GAGE family of tumor antigens found by homology walking in the dbEST database. (PubMed id 10197611)³ Brinkmann U.... Pastan I. (1999)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 170626	HGNC: 14618	AceView: GAGED4	Ensembl:ENSG00000171402	euGenes: HUgn170626
ECgene: XAGE3	H-InvDB: XAGE3

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
ATLAS Chromosomes in Cancer entry for XAGE3	Genetics and Cytogenetics in Oncology and Haematology

Licensable Technologies for XAGE3
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
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Products for XAGE3:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for XAGE3-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for XAGE3		Browse antibodies at Sigma-Aldrich
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for XAGE3		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

	Antibodies		Primer Pairs
	Cell Culture Products		ELISAs
	Flow Cytometry Kits		Protease Activity Assays & Reagents
	Cell Selection/Detection Kits & Reagents		ELISA/Assay Development Kits & Reagents
	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

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	Custom Service for Rabbit Pab from Full-length Protein

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Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies

Search Novus for antibodies for XAGE3

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XAGE3 Gene

protein-coding GIFtS: 40

Aliases &
Descriptions
for XAGE3

Summaries
for XAGE3

Genomic Location
for XAGE3

Proteins
for XAGE3

Protein Domains/
Families
for XAGE3

Gene Function
for XAGE3

Pathways & Interactions
for XAGE3

Drugs & Compounds
for XAGE3

Transcripts
for XAGE3

Expression
for XAGE3

Orthologs
for XAGE3

Paralogs
for XAGE3

SNPs/Variants
for XAGE3

Disorders & Mutations
for XAGE3

Medical News
for XAGE3

Publications
for XAGE3

Search for XAGE3

Genome Databases showing XAGE3

Other Databases showing XAGE3

Specialized Databases showing XAGE3

Licensable Technologies
for XAGE3

Services
for XAGE3

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

XAGE3 Gene

protein-coding GIFtS: 40

Aliases & Descriptionsfor XAGE3

Summariesfor XAGE3

Genomic Locationfor XAGE3

Proteinsfor XAGE3

Protein Domains/Familiesfor XAGE3

Gene Functionfor XAGE3

Pathways & Interactionsfor XAGE3

Drugs & Compoundsfor XAGE3

Transcriptsfor XAGE3

Expression for XAGE3

Orthologsfor XAGE3

Paralogsfor XAGE3

SNPs/Variantsfor XAGE3

Disorders & Mutationsfor XAGE3

Medical Newsfor XAGE3

Publicationsfor XAGE3

Search for XAGE3

Genome Databases showing XAGE3

Other Databases showing XAGE3

Specialized Databases showing XAGE3

Licensable Technologiesfor XAGE3

Servicesfor XAGE3

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for XAGE3

Summaries
for XAGE3

Genomic Location
for XAGE3

Proteins
for XAGE3

Protein Domains/
Families
for XAGE3

Gene Function
for XAGE3

Pathways & Interactions
for XAGE3

Drugs & Compounds
for XAGE3

Transcripts
for XAGE3

Expression
for XAGE3

Orthologs
for XAGE3

Paralogs
for XAGE3

SNPs/Variants
for XAGE3

Disorders & Mutations
for XAGE3

Medical News
for XAGE3

Publications
for XAGE3

Licensable Technologies
for XAGE3

Services
for XAGE3