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XAGE1D Gene

protein-coding GIFtS: 38

GC0XM052545

X antigen family, member 1D
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases
CT12.1 ^³
CT12.1d ^¹, ²
GAGED2 ^³
OTTHUMP00000042367 ^²
XAGE1A ^³

Descriptions
Cancer/testis antigen 12.1 ^³
G antigen, family D, 2 ^²
Protein XAGE-1 ^³
X antigen family, member 1D ^²
cancer/testis antigen family 12, member 1d ^²

External Ids
HGNC: 21508¹
Entrez Gene: 9503²
UniProtKB: Q9HD64³
Ensembl: ENSG00000204376⁷

Search outside databases for aliases for XAGE1D gene

Previous GC identifer: GC0XU900701

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for XAGE1D:

This gene is a member of the XAGE subfamily, which belongs to the GAGE family. The GAGE genes are

expressed in a variety of tumors and in some fetal and reproductive tissues. This gene is strongly

expressed in Ewing's sarcoma, alveolar rhabdomyosarcoma and normal testis. The protein

encoded by this gene contains a nuclear localization signal and shares a sequence similarity with

other GAGE/PAGE proteins. Because of the expression pattern and the sequence similarity, this

protein also belongs to a family of CT (cancer-testis) antigens. Alternative splicing of this

gene, in addition to the use of an alternative transcription start site and an alternative

translation initiation codon, results in multiple variants that encode different isoforms.

[provided by RefSeq]

UniProtKB/Swiss-Prot: GAGD2_HUMAN, Q9HD64

Function: Unknown

Gene Wiki entry for XAGE1D

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:

UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: Xp11.22 Ensembl cytogenetic band: Xp11.22 HGNC cytogenetic band: Xp11.22

XAGE1D Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome X GeneLoc Exon Structure

GeneLoc location for GC0XM052545: (about GC identifiers)

Start:	52,544,884 bp from pter
End:	52,550,020 bp from pter
Size:	5,137 bases
Orientation:	minus strand

RefSeq DNA sequence:

NC_000023.9 NT_011630.14

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: GAGD2_HUMAN, Q9HD64 (See protein sequence)

Recommended Name: G antigen family D member 2

Size: 160 amino acids; 17751 Da

Secondary accessions: A6NJ94 Q5JPP0 Q5JPP3 Q8WWG5 Q8WWG6 Q969J6

Alternative splicing: 3 isoforms: Q9HD64-3 Q9HD64-2 Q9HD64-4

REFSEQ proteins (3 alternative transcripts):

NP_065144.1 NP_597673.1 NP_597674.1

ENSEMBL proteins:

ENSP00000364728 ENSP00000364097 ENSP00000364722 ENSP00000379755

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Recombinant Proteins from Abcam (XAGE1)
	Human Recombinant Proteins from Abnova (XAGE1D)

Browse Origene for full length recombinant human proteins expressed in human HEK293 cells

Antibodies for XAGE1D:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse Antibodies at Sigma-Aldrich
	Browse R&D Systems for Antibodies
	Antibodies from Abcam (XAGE1), each with their Abpromise^SM
	Monoclonal and Polyclonal Antibodies from Abnova (XAGE1D)
	Novus Biologicals Antibodies for XAGE1D

Assays for XAGE1D:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

Graphical View of Domain Structure for InterPro Entry Q9HD64

ProtoNet protein and cluster: Q9HD64

1 Blocks protein family: IPB008625 GAGE

UniProtKB/Swiss-Prot: GAGD2_HUMAN, Q9HD64

Similarity: Belongs to the GAGE family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (XAGE1D)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 4): NM_020411

Applied Biosystems Silencer® siRNAs for XAGE1D

Sigma-Aldrich siRNA for XAGE1D

Sigma-Aldrich shRNA for XAGE1D

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Invitrogen Clones for XAGE1D
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_020411
Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_020411
untagged cDNA clones in CMV expression vector (see all 4): BC009538

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_133431

UniProtKB/Swiss-Prot: GAGD2_HUMAN, Q9HD64

Function: Unknown

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for XAGE1D

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (XAGE1D)
		Browse for Gene Knock-down Tools from Millipore
		Browse Abnova for Chimera RNAi Products

OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 4): NM_020411

Sigma-Aldrich siRNA for XAGE1D

Sigma-Aldrich shRNA for XAGE1D

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_020411 NM_133430 NM_133431

REFSEQ mRNAs for XAGE1D gene (3 alternative transcripts):

NM_020411.2 NM_133430.2 NM_133431.3

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_020411 NM_133430 NM_133431

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 3): NM_020411
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 3): NM_020411
                                 untagged cDNA clones in CMV expression vector (see all 4): BC009538

Additional cDNA sequence:

AJ290447.1 AJ318879.1

13 DOTS entries:

DT.100021393 DT.411576 DT.91677790 DT.100736412 DT.91677782 DT.97825377 DT.121280905 DT.121280912

DT.411577 DT.99982060 DT.121290883 DT.121290887 DT.91677799

24/45 AceView cDNA sequences (see all 45 ):

AJ318878 AA620697 BE881023 AA948244 NM_020411 BX101350 AJ318879 AW386187

NM_133430 BC009538 AF251237 NM_133431 BU564534 AI631681 AJ290447 AI954712

BU182421 BE875341 AA758618 BT007099 BG217244 BI460303 BG216048 AA483341

Unigene Clusters for XAGE1D:

X antigen family, member 1D

Hs.112208 [show with all ESTs], Hs.584511

Unigene Representative Sequences: BG191783, NM_133430

GeneLoc Exon Structure

4 Ensembl transcripts including schematic representations:

ENST00000375578 ENST00000374959 ENST00000375572 ENST00000396497

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

XAGE1D expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for XAGE1D

¹ / ²

3 probe-sets matching XAGE1D gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
56998_at²	U95-B	5	1.00	0.20	1.00	1.00	--	--	--	--	--
220057_at²	U133-A	5	1.00	0.20	--	--	--	--	--	--	--
220057_at²	U133Plus2	5	1.00	0.20	--	--	--	--	--	--	--

About this table

Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene clusters: Hs.112208 Hs.584511
UniProtKB/Swiss-Prot: GAGD2_HUMAN, Q9HD64
Tissue specificity: In normal tissues, highly expressed in testis. Expressed also in many different
types of cancers: highly expressed in breast cancer, prostate cancer and many types of lung
cancers, including squamous cell carcinoma, small cell carcinoma, non-small cell carcinoma, and
adenocarcinoma, as well as in Ewing's cell lines, in some Ewing's sarcoma patient samples, and in
one of one alveolar rhabdomyosarcoma patient sample

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for XAGE1D gene from 1 species

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
chimpanzee (Pan troglodytes)	XAGE1A¹	--	X antigen family, member 1A	98.75(n) 96.87(a)	741118 XM_001143525.1 XP_001143525.1

About this table Species with no ortholog for XAGE1D

ENSEMBL Gene Tree for XAGE1D

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for XAGE1D gene

XAGE1E¹ ² XAGE1B² XAGE1A² XAGE1C²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 300289

10/11 Novoseek disease relationships for XAGE1D gene (see all 11 )

Disease	Score	Articles	PubMed IDs for Articles with Shared Sentences (# sentences)
adenocarcinoma lung	71.43	4	11753648 (3)
ewings sarcoma	71.01	3	11753648 (1), 11774264 (1), 12479262 (1)
testicular cancer	70.64	3	11753648 (1), 12479262 (1), 17335148 (1)
small cell lung cancer	52.31	1	12479262 (1)
cancer lung	48.83	2	12479262 (1), 17335148 (1)
melanoma	29.83	2	11774264 (1), 12479262 (1)
adenocarcinoma	28.10	1	12479262 (1)
prostate cancer	27.79	7	15905609 (4), 12479262 (1)
cancer	26.01	7	12479262 (2), 11774264 (1), 16061866 (1), 15905609 (1)
metastasis	12.24	4	11774264 (2), 15905609 (1)

About this table

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

10/13 PubMed articles for XAGE1D gene (see all 13 ):

Characterization of overlapping XAGE-1 transcripts encoding a cancer testis antigen expressed in lung, breast, and other types of cancers. (PubMed id 12479262)^{1, 3, 4} Egland K.A.... Pastan I. (2002)
The DNA sequence of the human X chromosome. (PubMed id 15772651)^{3, 4} Ross M.T.... Bentley D.R. (2005)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
The XAGE family of cancer/testis-associated genes: alignment and expression profile in normal tissues, melanoma lesions and Ewing's sarcoma. (PubMed id 11992404)^{3, 4} Zendman A.J.W....van Muijen G.N.P. (2002)
XAGE-1, a new gene that is frequently expressed in Ewing's sarcoma. (PubMed id 10987281)^{3, 4} Liu X.F.... Pastan I. (2000)
XAGE-1 expression in non-small cell lung cancer and antibody response in patients. (PubMed id 16061866)^{1, 3} Nakagawa K....Nakayama E. (2005)
Characterization of XAGE-1b, a short major transcript of cancer/testis-associated gene XAGE-1, induced in melanoma metastasis. (PubMed id 11774264)^{1, 3} Zendman A.J....van Muijen G.N. (2002)
Identification of XAGE-1 isoforms: predominant expression of XAGE-1b in testis and tumors. (PubMed id 17335148)^{1, 3} Sato S....Nakayama E. (2007)
Activation of human cancer/testis antigen gene, XAGE-1, in tumor cells is correlated with CpG island hypomethylation. (PubMed id 15800911)³ Lim J.H....Kwon T.K. (2005)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 9503	HGNC: 21508	AceView: GAGED2.3	Ensembl:ENSG00000204376	euGenes: HUgn9503
ECgene: XAGE1D	H-InvDB: XAGE1D

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Licensable Technologies for XAGE1D
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
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Products for XAGE1D:

TaqMan ® Gene Expression Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for XAGE1D-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for XAGE1D		Browse antibodies at Sigma-Aldrich
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for XAGE1D		Browse small molecules at Sigma-Aldrich
	"Your Favorite Gene" Pathways		feedback

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	Cell Culture Products		ELISAs
	Flow Cytometry Kits		Protease Activity Assays & Reagents
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	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
	Recombinant & Natural Proteins

	Recombinant Proteins (XAGE1D)
	Antibodies (XAGE1D)
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	Custom Service for Rabbit Pab from Full-length Protein

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Search Tocris compounds for XAGE1D

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Antibodies for XAGE1D

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XAGE1D Gene

protein-coding GIFtS: 38

Aliases &
Descriptions
for XAGE1D

Summaries
for XAGE1D

Genomic Location
for XAGE1D

Proteins
for XAGE1D

Protein Domains/
Families
for XAGE1D

Gene Function
for XAGE1D

Pathways & Interactions
for XAGE1D

Drugs & Compounds
for XAGE1D

Transcripts
for XAGE1D

Expression
for XAGE1D

Orthologs
for XAGE1D

Paralogs
for XAGE1D

SNPs/Variants
for XAGE1D

Disorders & Mutations
for XAGE1D

Medical News
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Publications
for XAGE1D

Search for XAGE1D

Genome Databases showing XAGE1D

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Specialized Databases showing XAGE1D

Licensable Technologies
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Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

XAGE1D Gene

protein-coding GIFtS: 38

Aliases & Descriptionsfor XAGE1D

Summariesfor XAGE1D

Genomic Locationfor XAGE1D

Proteinsfor XAGE1D

Protein Domains/Familiesfor XAGE1D

Gene Functionfor XAGE1D

Pathways & Interactionsfor XAGE1D

Drugs & Compoundsfor XAGE1D

Transcriptsfor XAGE1D

Expression for XAGE1D

Orthologsfor XAGE1D

Paralogsfor XAGE1D

SNPs/Variantsfor XAGE1D

Disorders & Mutationsfor XAGE1D

Medical Newsfor XAGE1D

Publicationsfor XAGE1D

Search for XAGE1D

Genome Databases showing XAGE1D

Other Databases showing XAGE1D

Specialized Databases showing XAGE1D

Licensable Technologiesfor XAGE1D

Servicesfor XAGE1D

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for XAGE1D

Summaries
for XAGE1D

Genomic Location
for XAGE1D

Proteins
for XAGE1D

Protein Domains/
Families
for XAGE1D

Gene Function
for XAGE1D

Pathways & Interactions
for XAGE1D

Drugs & Compounds
for XAGE1D

Transcripts
for XAGE1D

Expression
for XAGE1D

Orthologs
for XAGE1D

Paralogs
for XAGE1D

SNPs/Variants
for XAGE1D

Disorders & Mutations
for XAGE1D

Medical News
for XAGE1D

Publications
for XAGE1D

Licensable Technologies
for XAGE1D

Services
for XAGE1D