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GPN1 Gene

protein-coding   GIFtS: 50

GC02P027705
GPN-loop GTPase 1
(Previous names: XPA binding protein 1, XPA binding protein 1, GTPase )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: XAB1)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
ATPBD1A 1, 2
FLJ51176 2
MBDIN 1, 2, 3
MBDin 3
NTPBP 1, 2
XAB1 2, 3
Descriptions
ATP(GTP)-binding protein 2
GPN-loop GTPase 1 2
MBD2-interacting protein 2, 3
XPA binding protein 1 1
XPA binding protein 1, GTPase 1, 2
XPA-binding protein 1 3
External Ids
HGNC: 170301
Entrez Gene: 113212
UniProtKB: Q9HCN43
Ensembl: ENSG000001152547
Search outside databases for aliases for GPN1 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for GPN1:
This gene encodes a guanosine triphosphatase enzyme. The encoded protein may play a role in DNA
repair and may function in activation of transcription. Alternatively spliced transcript variants
have been described. [provided by RefSeq]

UniProtKB/Swiss-Prot: GPN1_HUMAN, Q9HCN4
Function: Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding
protein, suggesting that it is required to connect RNA polymerase II to regulators of protein
complex formation. May be involved in nuclear localization of XPA

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 2p23.3   Ensembl cytogenetic band:  2p23.2   HGNC cytogenetic band: 2p23.3

GPN1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02P027705:     (about GC identifiers)

Start:
27,705,029 bp from pter
End:
27,727,217 bp from pter
Size:
22,189 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000002.10  NT_022184.14  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: GPN1_HUMAN, Q9HCN4 (See protein sequence)
Recommended Name: GPN-loop GTPase 1  
Size: 374 amino acids; 41740 Da
Subunit: Tightly associated with the RNA polymerase II complex. Interacts with XPA
Subcellular location: Cytoplasm
Secondary accessions: O76004

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins (4 alternative transcripts): 
    NP_001138519.1  NP_001138520.1  NP_001138521.1  NP_009197.2  


    ENSEMBL proteins: 
    ENSP00000384255 ENSP00000264718 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (XAB1)
    Human Recombinant Proteins from Abnova (XAB1)
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737 cytoplasm IEA--
    About this table

    Antibodies for GPN1: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibody Arrays and Antibodies for GPN1
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (XAB1), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (XAB1)
    Novus Biologicals Antibodies for GPN1

    Assays for GPN1: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    2 InterPro domains/families:
     IPR004130 DUF_ATP_bd
     IPR003593 ATPase_AAA+_core


       GeneDecks  GPN1 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9HCN4

    ProtoNet protein and cluster: Q9HCN4

    2 Blocks protein families:
    IPB001806 Transforming protein P21 RAS signature
    IPB004130 Conserved hypothetical ATP binding protein


    UniProtKB/Swiss-Prot: GPN1_HUMAN, Q9HCN4
    Similarity: Belongs to the GPN-loop GTPase family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (GPN1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (XAB1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 5): NM_007266

                  Applied Biosystems Silencer® siRNAs for GPN1

                  Sigma-Aldrich siRNA for GPN1  
                         Sigma-Aldrich shRNA for GPN1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Invitrogen Clones for GPN1
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 4): NM_001145047
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 4): NM_001145047
                                     untagged cDNA clones in CMV expression vector (see all 4): NM_001145047 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_007266

    UniProtKB/Swiss-Prot: GPN1_HUMAN, Q9HCN4
    Function: Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding
    protein, suggesting that it is required to connect RNA polymerase II to regulators of protein
    complex formation. May be involved in nuclear localization of XPA

    1 MGI phenotypic allele for Gpn1 (no phenotypes)


    4 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166 nucleotide binding IEA--
    GO:0005515 protein binding IPI11058119
    GO:0005525 GTP binding IEA--
    GO:0017111 nucleoside-triphosphatase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    2 Interacting proteins for GPN1 (Q9HCN41, 2) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    XPAP230251EBI-745137, EBI-295222
    ZBED1O960062MINT-66624
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for GPN1

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (GPN1)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (XAB1)
                   OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 5): NM_007266

                  Sigma-Aldrich siRNA for GPN1  
                         Sigma-Aldrich shRNA for GPN1  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_007266  

    REFSEQ mRNAs for GPN1 gene (4 alternative transcripts): 

    NM_001145047.1   NM_001145048.1   NM_001145049.1   NM_007266.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_007266  

                   OriGene GFP tagged cDNA clones in CMV expression vector (see all 4): NM_001145047
                                     Myc/DDK tagged cDNA clones in CMV expression vector (see all 4): NM_001145047
                                     untagged cDNA clones in CMV expression vector (see all 4): NM_001145047 

    Additional cDNA sequence: 

    AB044661.1 AJ010842.1 AK298827.1 AK298866.1 AK302131.1 AK310340.1 AK311423.1 AK312992.1 
    AK316404.1 BC007451.2 CR602623.1 CR608298.1 CR612976.1 CR617699.1 

    9 DOTS entries:

    DT.100802471  DT.100802465  DT.100675496  DT.448385  DT.95135337  DT.95114895  DT.100672702  DT.100802468 
    DT.100802475 

    24/238 AceView cDNA sequences (see all 238 ):

    BC007451 AW005665 CA416162 BG419532 BU149889 AA448819 AA291318 BI752213 
    BX462192 BM810685 AI382707 BQ215762 AA181995 BQ427528 CD107295 AW073406 
    BM461473 AA884754 CR612976 AW169849 BU166962 AA448818 CR608298 BX441317 

    Unigene Cluster for GPN1:

    GPN-loop GTPase 1
    Hs.18259  [show with all ESTs]
    Unigene Representative Sequence: NM_007266


    GeneLoc Exon Structure

    5/6 Alternative Splicing Database (ASD) splice patterns (SP) for GPN1 (see all 6 )

    ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9a · 9b ^ 10 ^ 11 ^ 12a · 12b ^ 13 ^ 14a · 14b
    SP1:                                      -                                                                                       
    SP2:                                      -                                                     -                                 
    SP3:                                -     -     -                                                                                 
    SP4:                                -     -     -     -                                                                           
    SP5:                                                                                                              -               

    About this scheme

    ECgene alternative splicing isoforms for GPN1

    2 Ensembl transcripts including schematic representations:
    ENST00000407583  ENST00000264718  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    GPN1 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for GPN1

    1 / 2

    3 probe-sets matching GPN1 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    41756_at2 U95-A 1 1.00 1.00 1.00 1.00 -- -- -- -- --

    209313_at2 U133-A 1 1.00 1.00 -- -- -- -- -- -- --

    209313_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: ATTAATGAAT

    SOURCE GeneReport for Unigene cluster: Hs.18259

    UniProtKB/Swiss-Prot: GPN1_HUMAN, Q9HCN4
    Tissue specificity: Expressed ubiquitously

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for GPN1 gene from 5/25 species (see all 25 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    LOC6083081   -- similar to XPA binding protein 1 91.27(n)
    95.19(a)
    608308  XM_845410.1  XP_850503.1 
    chimpanzee
    (Pan troglodytes)
    GPN11   -- GPN-loop GTPase 1 99.38(n)
    98.93(a)
    470341  XM_525723.2  XP_525723.2 
    cow
    (Bos taurus)
    GPN11   -- GPN-loop GTPase 1 92.2(n)
    93.82(a)
    508522  XM_585310.3  XP_585310.2 
    mouse
    (Mus musculus)
    Gpn11, 5 55
    GPN-loop GTPase 11, 5 86.97(n)1
    91.11(a)1
    742541  NM_133756.31  NP_598517.11 
     AA2045825  AI4496155  (see all 16)
    chicken
    (Gallus gallus)
    XAB11   -- XPA binding protein 1, GTPase 74.93(n)
    80.99(a)
    421980  XM_419990.1  XP_419990.1 
    About this table        Species with no ortholog for GPN1

    ENSEMBL Gene Tree for GPN1
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for GPN1 gene
    GPN22  GPN32  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/98 NCBI SNPs in GPN1 are shown (see all 98 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 60)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 2 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs67189551,2
    A,C,F,H27718829(+) AGAGAA/GCAACT 1 E/E syn14Minor allele frequency- G:0.04EU EA WA 404
    rs15284021,2
    A,C,F,H,O27711021(+) CCATTA/GGCGTG 1 -- int112Minor allele frequency- G:0.36MN NA EU EA WA 1106
    rs46660051,2
    A,C,F,H27708743(+) ATAACG/AACTTG 1 -- int115Minor allele frequency- A:0.35NA EU EA WA 1400
    rs46660091,2
    A,C,F,H27711496(+) TGATTA/TTTTTG 1 -- int14Minor allele frequency- T:0.28EU EA WA 420
    rs21781971,2
    A,C,F,H27714055(-) ctgtaT/Cagtct 1 -- int17Minor allele frequency- C:0.38EU EA WA NA 560
    rs101737201,2
    A,C,F,H27708901(+) GAGGGA/TTTCAG 1 -- int18Minor allele frequency- T:0.34EA NA EU WA 636
    rs65477411,2
    A,C,F,H27709428(+) TGTCAA/GAATAG 1 -- int17Minor allele frequency- G:0.33EU EA WA NA 560
    rs46660041,2
    A,C,F,H27708556(+) GGTATA/TTGGGA 1 -- int14Minor allele frequency- T:0.38EU EA WA 416
    rs67121331,2
    A,C,F,H27720778(+) gttcaC/Tggtgt 1 -- int17Minor allele frequency- T:0.05EU EA WA NA 560
    rs65477401,2
    A,C,F,H27707873(+) GCTTGA/GTTTAT 1 -- int115Minor allele frequency- G:0.32NA EU EA WA 1338
    About this table

    HapMap Linkage Disequilibrium images for GPN1 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 611479

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/14 PubMed articles for GPN1 gene (see all 14 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 11321 HGNC: 17030 AceView: XAB1 Ensembl:ENSG00000115254 euGenes: HUgn11321
    ECgene: GPN1 H-InvDB: GPN1
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for GPN1:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for GPN1 Antibodies for GPN1
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for GPN1 Browse small molecules at Sigma-Aldrich
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     Antibodies for GPN1

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