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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

WT1-AS Gene

RNA gene GIFtS: 36
GCID: GC11P032459

WT1 antisense RNA

(Previous names: Wilms tumor upstream neighbor 1, WT1 antisense RNA (non-protein...)
(Previous symbol: WIT1)

Explore 6 diseases affiliated with
WT1-AS via

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our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Subcategory (RNA class): lncRNA

Quality score for this RNA gene is 3

Aliases
WT1 Antisense RNA¹ ²		Wilms Tumor Upstream Neighbor 1¹
WIT-1¹ ² ³		WT1 Antisense RNA (Non-Protein Coding)¹
WIT1¹ ² ³		DJ74J1.1¹
WT1-AS1¹ ²		Wilms Tumor-Associated Antisense RNA³
WT1AS¹ ²

External Ids:	HGNC: 18135¹	Entrez Gene: 51352²	Ensembl: ENSG00000183242⁷	OMIM: 607899⁵	UniProtKB: Q06250³

ORGUL members:
	NONCODE:n380247

Export aliases for WT1-AS gene to outside databases

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for WT1-AS:

This gene is located upstream of the Wilms tumor 1 (WT1) gene; these two genes are bi-directionally transcribed from

the same promoter region. The WT1 gene is a tumor suppressor gene that has been implicated in Wilms tumor, and it is

expressed in cells of mesodermal origin. This gene and the WT1 gene have the same temporal and cell-restricted

expression pattern, although the expression of this gene is less abundant than WT1 expression. WT1 is involved in the

development of the urogenital system, whereas the role of this gene in embryogenesis is unclear. This gene appears to

produce antisense RNA transcripts that help regulate the WT1 gene. This gene is imprinted in kidney, with preferential

expression from the paternal allele, and it has been proposed that imprinting defects at chromosome 11p13 may

contribute to Wilms' tumourigenesis. (provided by RefSeq, Oct 2010)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000011.9 NC_018922.1 NT_009237.18

Regulatory elements:

Search SABiosciences Regulatory transcription factor binding sites for WT1-AS
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: WT1-AS promoter sequence

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Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat WT1-AS

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 11p13 Ensembl cytogenetic band: 11p13 HGNC cytogenetic band: 11p13

WT1-AS Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
WT1-AS gene location

GeneLoc information about chromosome 11 GeneLoc Exon Structure

GeneLoc location for GC11P032459: view genomic region (about GC identifiers)

Start:	32,457,064 bp from pter	End:	32,480,315 bp from pter
Size:	23,252 bases	Orientation:	plus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
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About This Section

UniProtKB/Swiss-Prot: WIT1_HUMAN, Q06250 (See protein sequence)

Recommended Name: Putative Wilms tumor upstream neighbor 1 gene protein

Size: 92 amino acids; 10039 Da

Caution: Product of a dubious CDS prediction

Sequence caution: Sequence=CAA49572.1; Type=Frameshift; Positions=83;

Secondary accessions: Q4KMY0 Q96A27

Explore the universe of human proteins at neXtProt for WT1-AS: NX_Q06250

WT1-AS Protein expression data from MOPED and PaxDb: --

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Gene Ontology (GO): 1 cellular component term (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005575	cellular_component	ND	--

WT1-AS for ontologies About GeneDecksing

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(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

ProtoNet protein and cluster: Q06250

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

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Gene Ontology (GO): 1 molecular function term (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0003674	molecular_function	ND	--

WT1-AS for ontologies About GeneDecksing

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Interactions:

Search SABiosciences Gene Network Central^TM Interacting Genes and Proteins Networks for WT1-AS

Gene Ontology (GO): 1 biological process term (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0008150	biological_process	ND	--

WT1-AS for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

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(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
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DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
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About This Section

REFSEQ mRNAs for WT1-AS gene:

NM_015855.3

Unigene Cluster for WT1-AS:

WT1 antisense RNA

Hs.567499 [show with all ESTs]

Unigene Representative Sequence: DQ289489

7 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000494911 ENST00000395900(uc010rec.2 uc010red.2) ENST00000459866

ENST00000525436 ENST00000478367 ENST00000442957 ENST00000426618(uc021qfr.1)

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Additional cDNA sequence:

BC002734.2 BC096708.1 BC098290.1 BC100673.1 DQ289489.1 DQ289490.1 M60614.1 NR_023920.1

5 DOTS entries:

DT.408359 DT.100765578 DT.97768268 DT.100768343 DT.91982072

24/27 AceView cDNA sequences (see all 27):

CR604547 BU633051 NM_015855 AW028595 T54801 BE394437 AI648530 BM906117

BM545678 BC002734 AI951613 L25110 BM543913 BX110205 CA430691 M60614

BV196067 CB267195 BX394943 AA912908 AI803567 BI837715 AW194904 BX390192

GeneLoc Exon Structure

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

Expression evidence for WT1-AS:none

WT1-AS expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: AAATTGATGA

About this image

WT1-AS expression in embryonic tissues and stem cells

Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

Stem Cell Differentiation: 1 LifeMap Cell

Name

Category

Beating cell clusters (Spontaneous differen...)

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

Genevestigator expression for WT1-AS

SOURCE GeneReport for Unigene cluster: Hs.567499

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(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

--

ENSEMBL Gene Tree for WT1-AS (if available)
TreeFam Gene Tree for WT1-AS (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 11 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs3809062^1,2	C,F,A,H,	--	32459082(+)	`CAACTA/GTGAGG`	1	--	int¹		10		NA WA CSA EA	372
rs183367102^1,2		--	32459091(+)	`GGGGCA/GAGGAG`	1	--	int¹		0	--	--	--	--
rs186957871^1,2		--	32459127(+)	`CATTTA/CCAAAC`	1	--	int¹		0	--	--	--	--
rs72893539^1,2	C,F,	--	32459132(+)	`CCAAAC/TGAAAA`	1	--	int¹		1		NA	120
rs148862948^1,2		--	32459186(+)	`GGGGGA/GAGAGG`	1	--	int¹		0	--	--	--	--
rs191471435^1,2		--	32459187(+)	`GGGGGA/GGAGGA`	1	--	int¹		0	--	--	--	--
rs116625991^1,2	F,	--	32459210(+)	`GGAGGC/TTGGCT`	1	--	int¹		1		WA	118
rs4140413^1,2	C,F,A,H,	--	32459228(-)	`CACAAA/CGACTC`	1	--	int¹		7		NA WA CSA EA	366
rs184128714^1,2		--	32459250(+)	`CAGGTA/GAGGCA`	1	--	int¹		0	--	--	--	--
rs11031782^1,2	C,F,	--	32459303(+)	`TTGCCC/GCGGGG`	1	--	int¹		8		NA WA CSA EA	249

HapMap Linkage Disequilibrium report for WT1-AS (32457064 - 32480315 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for WT1-AS: --
Human Gene Mutation Database (HGMD): WT1-AS

SABiosciences Cancer Mutation PCR Assays

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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

WT1-AS for disorders           About GeneDecksing

OMIM gene information: 607899    OMIM disorders: --

6 diseases for WT1-AS:    About MalaCards

wilms tumor focal segmental glomerulosclerosis glomerulosclerosis acute myeloid leukemia

myeloid leukemia leukemia

Export disorders for WT1-AS gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for WT1-AS gene integrated from 9 sources:
(articles sorted by number of sources associating them with WT1-AS)

Utopia: connect your pdf to the dynamic
world of online information

Sequence of the WT1 upstream region including the Wit-1 gene. (PubMed id 8406502)^{1, 2, 3} Gassler M. and Bruns G.A.P. (1993)
Tissue, developmental, and tumor-specific expression of divergent transcripts in Wilms tumor. (PubMed id 2173145)^{1, 2, 3} Huang A....Williams B.R. (1990)
A CTCF-binding silencer regulates the imprinted genes AWT1 and WT1-AS and exhibits sequential epigenetic defects during Wilms' tumourigenesis. (PubMed id 17210670)^{1, 3} Hancock A.L....Malik K. (2007)
Human chromosome 11 DNA sequence and analysis including novel gene identification. (PubMed id 16554811)^{1, 2} Taylor T.D....Sakaki Y. (2006)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
Restriction landmark genome scanning for aberrant methylation in primary refractory and relapsed acute myeloid leukemia; involvement of the WIT-1 gene. (PubMed id 10340388)^{1, 9} Plass C....Caligiuri M.A. (1999)
Cloning of novel Wilms tumor gene (WT1) cDNAs; eviden ce for antisense transcription of WT1. (PubMed id 8208551)^{1, 9} Eccles M.R....Reeve A.E. (1994)
Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)¹ Kimura K.... Sugano S. (2006)
Variants in the Wilms' tumor gene are associated with focal segmental glomerulosclerosis in the African American population. (PubMed id 15687485)¹ Orloff M.S....Kopp J.B. (2005)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)¹ Strausberg R.L....Marra M.A. (2002)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 51352	HGNC: 18135	AceView: WIT-1	Ensembl:ENSG00000183242	euGenes: HUgn51352
ECgene: WT1-AS	H-InvDB: WT1-AS

(According to HUGE)
About This Section

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for WT1-AS	Pharmacogenomics, SNPs, Pathways

Intellectual Property for WT1-AS gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

About This Section

Patent Information for WT1-AS gene:
Search GeneIP for patents involving WT1-AS

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
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	Variant Library Synthesis
	Codon Optimization
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Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for WT1-AS

	SwitchGear 3'UTR luciferase reporter plasmids for WT1-AS
	SwitchGear Promoter luciferase reporter plasmids for WT1-AS

Search ThermoFisher Antibodies for WT1-AS

Search Vector BioLabs for ready-to-use adenovirus/AAV for human, mouse, rat WT1-AS

GeneCards Homepage - Last full update: 19 Mar 2013 - Incrementals: 21 Mar 2013 , 15 Apr 2013 , 27 Apr 2013

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Category			VWF (GIFTS: 73) von Willebrand factor
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The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

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Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

Hot genes Disease genes WT1-AS gene at Home site.

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WT1-AS Gene

WT1 antisense RNA

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WT1-AS Gene

WT1 antisense RNA

Aliasesfor WT1-AS gene

Summariesfor WT1-AS gene

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External Searches for WT1-AS gene

Genome Databases showing WT1-AS gene

Other Databases showing WT1-AS gene

Specialized Databases showing WT1-AS gene

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Aliases
for WT1-AS gene

Summaries
for WT1-AS gene

Genomic Views
for WT1-AS gene

Proteins
for WT1-AS gene

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Families
for WT1-AS gene

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for WT1-AS gene

Pathways & Interactions
for WT1-AS gene

Drugs & Compounds
for WT1-AS gene

Transcripts
for WT1-AS gene

Expression
for WT1-AS gene

Orthologs
for WT1-AS gene

Paralogs
for WT1-AS gene

Genomic Variants
for WT1-AS gene

Disorders / Diseases
for WT1-AS gene

Publications
for WT1-AS gene

Intellectual Property
for WT1-AS gene

Products
for WT1-AS gene