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WRN Gene

protein-coding   GIFtS: 66
GCID: GC08P030948

Werner Syndrome, RecQ Helicase-Like

(Previous name: Werner syndrome)
  See WRN-related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Werner Syndrome, RecQ Helicase-Like1 2     RECQL32
RECQ32 3 5     Werner Syndrome ATP-Dependent Helicase2
RECQL22 3 5     EC 3.1.-.-3
Exonuclease WRN2 3     EC 3.6.4.123
RecQ Protein-Like 22 3     RecQ33
DNA Helicase, RecQ-Like Type 32 3     EC 3.6.18
Werner Syndrome1     

External Ids:    HGNC: 127911   Entrez Gene: 74862   Ensembl: ENSG000001653927   OMIM: 6046115   UniProtKB: Q141913   

Export aliases for WRN gene to outside databases

Previous GC identifers: GC08P030795 GC08P031342 GC08P031010 GC08P029435


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for WRN Gene:
This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA
helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication,
recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a
predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to
5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this
protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner
syndrome, an autosomal recessive disorder characterized by premature aging. (provided by RefSeq, Jul 2008)

GeneCards Summary for WRN Gene:
WRN (Werner syndrome, RecQ helicase-like) is a protein-coding gene. Diseases associated with WRN include werner syndrome, and rothmund-thomson syndrome. GO annotations related to this gene include protein complex binding and protein homodimerization activity. An important paralog of this gene is RECQL4.

UniProtKB/Swiss-Prot: WRN_HUMAN, Q14191
Function: Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5'
exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards
single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing
alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in
the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled
replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions.
Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably
associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand
break repair after gamma-irradiation

Gene Wiki entry for WRN (Werner syndrome ATP-dependent helicase) Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000008.10  NC_018919.2  NT_167187.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the WRN gene promoter:
         GATA-3   p300   AP-2beta   Nkx2-5   AP-2alpha   AP-2gamma   GATA-2   GATA-1   AP-2alphaA   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): WRN promoter sequence
   Search Chromatin IP Primers for WRN

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat WRN


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 8p12   Ensembl cytogenetic band:  8p12   HGNC cytogenetic band: 8p12

WRN Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
WRN gene location

GeneLoc information about chromosome 8         GeneLoc Exon Structure

GeneLoc location for GC08P030948:  view genomic region     (about GC identifiers)

Start:
30,890,778 bp from pter      End:
31,031,285 bp from pter
Size:
140,508 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: WRN_HUMAN, Q14191 (See protein sequence)
Recommended Name: Werner syndrome ATP-dependent helicase  
Size: 1432 amino acids; 162461 Da
Cofactor: Binds 2 magnesium ions per subunit. Has high activity with manganese and zinc ions (in vitro)
Subunit: Monomer, and homooligomer. May exist as homodimer, homotrimer, homotetramer and/or homohexamer.
Homotetramer, or homohexamer, when bound to DNA. Interacts via its N-terminal domain with WRNIP1 (By similarity).
Interacts with EXO1, PCNA and SUPV3L1. Interacts with PML (isoform PML-4)
Selected PDB 3D structures from and Proteopedia for WRN (see all 10):
2AXL (3D)        2DGZ (3D)        2E1E (3D)        2E1F (3D)        2FBT (3D)        2FBV (3D)    
Secondary accessions: A1KYY9

Explore the universe of human proteins at neXtProt for WRN: NX_Q14191

Explore proteomics data for WRN at MOPED

Post-translational modifications: 

  • Phosphorylated by PRKDC1
  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus
  • 2 DME Specific Peptides for WRN (Q14191)
     DEAHCIS  VIHYGAPK 


    See WRN Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_000544.2  
    ENSEMBL proteins: 
     ENSP00000298139  

    WRN Human Recombinant Protein Products:

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    Browse OriGene Protein Over-expression Lysates
    OriGene Custom MassSpec
    OriGene Custom Protein Services for WRN
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    Novus Biologicals WRN Protein
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for WRN

     
    Search eBioscience for Proteins for WRN 

     
    antibodies-online proteins for WRN (2 products) 

     
    antibodies-online peptides for WRN

    WRN Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of WRN
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    antibodies-online antibodies for WRN (38 products) 

    WRN Assay Products:

    Browse Kits and Assays available from EMD Millipore
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    Browse R&D Systems for biochemical assays
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    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for WRN
    Cloud-Clone Corp. CLIAs for WRN
    Search eBioscience for ELISAs for WRN 
    antibodies-online kits for WRN (3 products) 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    Selected InterPro protein domains (see all 11):
     IPR011545 DNA/RNA_helicase_DEAD/DEAH_N
     IPR004589 DNA_helicase_ATP-dep_RecQ
     IPR010997 HRDC-like
     IPR011991 WHTH_DNA-bd_dom
     IPR027417 P-loop_NTPase

    Graphical View of Domain Structure for InterPro Entry Q14191

    ProtoNet protein and cluster: Q14191

    2 Blocks protein domains:
    IPB002121 HRDC domain
    IPB002464 ATP-dependent helicase


    UniProtKB/Swiss-Prot: WRN_HUMAN, Q14191
    Similarity: Belongs to the helicase family. RecQ subfamily
    Similarity: Contains 1 3'-5' exonuclease domain
    Similarity: Contains 1 helicase ATP-binding domain
    Similarity: Contains 1 helicase C-terminal domain
    Similarity: Contains 1 HRDC domain


    Find genes that share domains with WRN           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: WRN_HUMAN, Q14191
    Function: Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5'
    exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards
    single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing
    alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in
    the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled
    replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions.
    Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably
    associates with foci elements generating binding sites for RP-A (By similarity). Plays a role in double-strand
    break repair after gamma-irradiation
    Catalytic activity: ATP + H(2)O = ADP + phosphate

         Genatlas biochemistry entry for WRN:
    DNA helicase(? 3'->5' or 5'>3') DNA containing exonuclease activity,ATP dependent, containing two transcription
    initiation sites,homologous to yeast Sgs1,E coli RecQ,C elegans F18C5.2 and X laevis focus forming activity 1
    (FFA1),involved in DNA unwinding function,in RNA polymerase II activation in DNA repair replication foci
    formation,maintaining genomic integrity,stimulated by replication proteins RPA1,RPA2,RPA3,upregulated at the G2/M
    stage of the cell cycle,predominantly localized in the nucleoplasm,RecQl family

         Enzyme Numbers (IUBMB): EC 3.6.12 EC 3.1.-.-1 EC 3.6.4.121

         Gene Ontology (GO): Selected molecular function terms (see all 23):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166nucleotide binding ----
    GO:0000287magnesium ion binding IDA16622405
    GO:0000403Y-form DNA binding IDA17715146
    GO:0000405bubble DNA binding IDA11433031
    GO:0003676nucleic acid binding ----
         
    Find genes that share ontologies with WRN           About GenesLikeMe


    Phenotypes:
         5 GenomeRNAi human phenotypes for WRN:
     Decreased viability of wild-ty  G0/1 arrest  Increased G1 DNA content  Increased HPV18 LCR reporter a 
     Lamellipodia cells 

         15 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Wrn):
     adipose tissue  cardiovascular system  cellular  digestive/alimentary  endocrine/exocrine gland 
     growth/size/body  homeostasis/metabolism  immune system  integument  limbs/digits/tail 
     mortality/aging  reproductive system  skeleton  tumorigenesis  vision/eye 

    Find genes that share phenotypes with WRN           About GenesLikeMe

    Animal Models:
         MGI mouse knock-out Wrntm1Led for WRN

       genOway: Develop your customized and physiologically relevant rodent model for WRN

    miRNA
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    Block miRNA regulation of human, mouse, rat WRN using miScript Target Protectors
    5 qRT-PCR Assays for microRNAs that regulate WRN:
    hsa-miR-3163 hsa-miR-4311 hsa-miR-369-3p hsa-miR-374b hsa-miR-374a
    SwitchGear 3'UTR luciferase reporter plasmidWRN 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for WRN
    Predesigned siRNA for gene silencing in human, mouse, rat WRN

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for WRN

    Clone
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    OriGene clones in human, mouse for WRN (see all 7)
    OriGene ORF clones in mouse, rat for WRN
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: WRN (NM_000553)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for WRN
    Search Vector BioLabs for ready-to-use adenovirus/AAV for human, mouse, rat WRN
    Addgene plasmids for WRN 

    Cell Line
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    Browse ESI BIO Cell Lines and PureStem Progenitors for WRN 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for WRN


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    WRN_HUMAN, Q14191: Nucleus, nucleolus. Nucleus. Nucleus, nucleoplasm. Note=Gamma-irradiation leads to its
    translocation from nucleoli to nucleoplasm and PML regulates the irradiation-induced WRN relocation
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytoskeleton5
    nucleus5
    cytosol2

    Gene Ontology (GO): Selected cellular component terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005622intracellular ----
    GO:0005634nucleus ----
    GO:0005654nucleoplasm IDA11420665
    GO:0005730nucleolus IDA11420665
    GO:0005813centrosome IDA17498979

    Find genes that share ontologies with WRN           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for WRN About    
    See pathways by source

    SuperPathContained pathways About
    1Telomere C-strand (Lagging Strand) Synthesis
    Cell cycle Transition and termination of DNA replication0.43
    2Non-homologous end-joining
    DNA damage NHEJ mechanisms of DSBs repair0.37
    3Cell Cycle / Checkpoint Control
    DNA Damage0.32
    4Regulation of Telomerase
    Regulation of Telomerase
    5Integrated Pancreatic Cancer Pathway
    Integrated Pancreatic Cancer Pathway


    Find genes that share SuperPaths with WRN           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 Cell Signaling Technology (CST) Pathway for WRN
        DNA Damage

    2 GeneGo (Thomson Reuters) Pathways for WRN
        DNA damage NHEJ mechanisms of DSBs repair
    Cell cycle Transition and termination of DNA replication

    2 BioSystems Pathways for WRN
        Integrated Pancreatic Cancer Pathway
    Regulation of Telomerase


        Pathway & Disease-focused RT2 Profiler PCR Arrays including WRN: 
              Stem Cell Transcription Factors in human mouse rat
              DNA Damage Signaling Pathway in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for WRN

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for WRN (Q141911, 2, 3 ENSP000002981394) via UniProtKB, MINT, STRING, and/or I2D (see all 164)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    RAD52P433511, 2, 3, ENSP000003512844EBI-368417,EBI-706448 MINT-6550741 MINT-17110 MINT-6550731 MINT-6550722 MINT-6550777 MINT-17109 MINT-6550709 MINT-6550679 MINT-6550697 MINT-6550759 I2D: score=4 STRING: ENSP00000351284
    H2AFXP161042, 3, ENSP000003643104MINT-56616 MINT-56619 MINT-56621 MINT-56626 MINT-56618 MINT-8048620 I2D: score=3 STRING: ENSP00000364310
    RPA2P159272, 3, ENSP000003630214MINT-8048594 MINT-8048571 I2D: score=1 STRING: ENSP00000363021
    BLMP541321, 3, ENSP000003472324EBI-368417,EBI-621372 I2D: score=4 STRING: ENSP00000347232
    TERF2Q155541, 3, ENSP000002549424EBI-368417,EBI-706637 I2D: score=4 STRING: ENSP00000254942
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 27):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000723telomere maintenance IMP18212065
    GO:0000731DNA synthesis involved in DNA repair IDA17563354
    GO:0001302replicative cell aging IEA--
    GO:0006139nucleobase-containing compound metabolic process ----
    GO:0006200ATP catabolic process IDA10373438

    Find genes that share ontologies with WRN           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for WRN

    1 HMDB Compound for WRN    About this table
    CompoundSynonyms CAS #PubMed Ids
    MagnesiumMagnesium (see all 2)7439-95-4--

    Selected Novoseek inferred chemical compound relationships for WRN gene (see all 17)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    camptothecin 60.5 11 15135730 (1), 17917271 (1), 18312465 (1), 16723399 (1) (see all 5)
    methylmethanesulfonate 59.2 18 18250621 (2), 15282207 (1), 20157511 (1), 16449207 (1) (see all 8)
    8-hydroxyadenine 55.8 1 11328876 (1)
    4nqo 55.4 3 10446247 (1), 19398855 (1), 17498979 (1)
    hydroxyurea 54.7 17 18250621 (2), 17173071 (2), 15135730 (1), 15282207 (1) (see all 9)
    8-oxoguanine 51.3 2 17611195 (1), 11328876 (1)
    polynucleotide 20.6 1 16822725 (1)
    atp 11.5 5 16793395 (2), 10212265 (1), 11717307 (1), 16503984 (1)
    bleomycin 7.53 10 15149862 (6), 12944467 (2)
    hydroquinone 6.49 27 19064679 (5), 17875398 (4), 18978339 (1)



    Find genes that share compounds with WRN           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for WRN gene: 
    NM_000553.4  

    Unigene Cluster for WRN:

    Werner syndrome, RecQ helicase-like
    Hs.632050  [show with all ESTs]
    Unigene Representative Sequence: NM_000553
    3 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000298139(uc011lbd.1 uc011lbe.1 uc003xio.4) ENST00000521620(uc010lvk.3)
    ENST00000520169
    miRNA
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    5 qRT-PCR Assays for microRNAs that regulate WRN:
    hsa-miR-3163 hsa-miR-4311 hsa-miR-369-3p hsa-miR-374b hsa-miR-374a
    SwitchGear 3'UTR luciferase reporter plasmidWRN 3' UTR sequence
    Inhib. RNA
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    GenScript: all cDNA clones in your preferred vector: WRN (NM_000553)
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    Primer
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    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat WRN
      QuantiTect SYBR Green Assays in human, mouse, rat WRN
      QuantiFast Probe-based Assays in human, mouse, rat WRN

    Additional mRNA sequence: 

    AB209652.1 AF091214.1 AL833572.1 AY818673.1 

    6 DOTS entries:

    DT.212373  DT.97770678  DT.75192400  DT.121484652  DT.40113162  DT.99977954 

    Selected AceView cDNA sequences (see all 49):

    BM471189 AI280329 AI277125 BM552381 BM721721 NM_000553 CB216263 BM785803 
    BQ774611 AI203498 BU633322 AL833572 BM677308 CD657076 N64051 BU683758 
    BP349077 BV183217 AA373157 BG941850 T39125 AF091214 CD654107 AA287923 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    WRN expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TAAAAAATGT
    WRN Expression
    About this image

    WRN Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    WRN Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.632050
        Pathway & Disease-focused RT2 Profiler PCR Arrays including WRN: 
              Stem Cell Transcription Factors in human mouse rat
              DNA Damage Signaling Pathway in human mouse rat

    Primer
    Products:
    OriGene qSTAR qPCR primer pairs in human, mouse for WRN
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat WRN
    QuantiTect SYBR Green Assays in human, mouse, rat WRN
    QuantiFast Probe-based Assays in human, mouse, rat WRN
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for WRN

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of eukaryotes.

    Orthologs for WRN gene from Selected species (see all 15)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Wrn1 , 5 Werner syndrome homolog (human)1, 5 79.48(n)1
    71.74(a)1
      8 (20.30 cM)5
    224271  NM_011721.41  NP_035851.31 
     376978085 
    chicken
    (Gallus gallus)
    Aves WRN1 Werner syndrome 64.04(n)
    56.59(a)
      422505  XM_001235032.3  XP_001235033.2 
    lizard
    (Anolis carolinensis)
    Reptilia WRN6
    Werner syndrome, RecQ helicase-like
    52(a)
    1 ↔ 1
    GL343257.1(447472-529197)
    African clawed frog
    (Xenopus laevis)
    Amphibia FFA-12 focus forming activity 1 74.34(n)    AF067418.1 
    zebrafish
    (Danio rerio)
    Actinopterygii wrn1 Werner syndrome 57.26(n)
    52.3(a)
      569495  XM_692888.6  XP_697980.5 
    worm
    (Caenorhabditis elegans)
    Secernentea wrn-11 , 3 wrn-11 36(a)3
    45.87(n)1
    34.78(a)1
      II(6554924-6559094)3
    1740811  NM_062923.51  NP_495324.21 
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes SGS16
    Nucleolar DNA helicase of the RecQ family; involve...
    17(a)
    1 → many
    XIII(640915-645258) YMR190C
    rice
    (Oryza sativa)
    Liliopsida Os07g06816001 Os07g0681600 46.54(n)
    36.18(a)
      4344306  NM_001067190.1  NP_001060655.1 


    ENSEMBL Gene Tree for WRN (if available)
    TreeFam Gene Tree for WRN (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for WRN gene
    RECQL42  RECQL52  RECQL2  BLM2  
    1 SIMAP similar gene for WRN using alignment to 1 protein entry:     WRN_HUMAN:
    RECQL

    Find genes that share paralogs with WRN           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for WRN (see all 2777)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 8 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    VAR_0363184
    A colorectal cancer sample4--see VAR_0363182 G V mis40--------
    VAR_0265894
    Werner syndrome (WRN)4--see VAR_0265892 K E mis40--------
    VAR_0265884
    Werner syndrome (WRN)4--see VAR_0265882 K N mis40--------
    rs1139939611,2
    Cpathogenic145077661(+) TAATAC/GGGTAG 1 -- spa10--------
    rs178475771,2
    C,Fpathogenic145138250(+) TGGAAC/TGAAAA 2 R * stg1 ese33Minor allele frequency- T:0.00NA EU 5873
    rs30874251,2,,4
    C,F,Hunknown145099085(+) ACATTC/TGCCAA 2 R C mis118Minor allele frequency- T:0.01NA MN NS EA 2040
    rs1832484571,2
    --29433638(+) TTTGGA/TAAGTC 1 -- us2k10--------
    rs1493316931,2
    C--29433793(+) AGTATC/TCTTAA 1 -- us2k10--------
    rs1853275391,2
    --29433929(+) ATACCA/GGAGGT 1 -- us2k10--------
    rs1445404021,2
    C--29433943(+) TTTCTA/GTTTCT 1 -- us2k10--------

    HapMap Linkage Disequilibrium report for WRN (30890778 - 31031285 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 3 variations for WRN:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv890706CNV Loss21882294
    nsv890705CNV Gain21882294
    nsv831278CNV Gain17160897

    Human Gene Mutation Database (HGMD): WRN
    Locus Specific Mutation Databases (LSDB): WRN

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing WRN
    DNA2.0 Custom Variant and Variant Library Synthesis for WRN

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 604611   
    OMIM disorders: 277700  
    UniProtKB/Swiss-Prot: WRN_HUMAN, Q14191
  • Werner syndrome (WRN) [MIM:277700]: A rare autosomal recessive progeroid syndrome characterized by the
    premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent
    diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is
    myocardial infarction. Note=The disease is caused by mutations affecting the gene represented in this entry
  • Colorectal cancer (CRC) [MIM:114500]: A complex disease characterized by malignant lesions arising from
    the inner wall of the large intestine (the colon) and the rectum. Genetic alterations are often associated with
    progression from premalignant lesion (adenoma) to invasive adenocarcinoma. Risk factors for cancer of the colon
    and rectum include colon polyps, long-standing ulcerative colitis, and genetic family history. Note=The disease
    may be caused by mutations affecting the gene represented in this entry

  • 7 diseases for WRN:    
    About MalaCards
    werner syndrome    rothmund-thomson syndrome    bloom syndrome    rapadilino syndrome
    progeria    spondylosis    colorectal cancer

    6 diseases from the University of Copenhagen DISEASES database for WRN:
    Werner syndrome     Bloom syndrome     Rothmund-Thomson syndrome     Progeria
    Rapadilino syndrome     Genetic disorder

    Find genes that share disorders with WRN           About GenesLikeMe

    Selected Novoseek inferred disease relationships for WRN gene (see all 24)    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    werner syndrome 99.1 355 12927431 (3), 15084309 (3), 15489508 (3), 20075015 (3) (see all 99)
    bloom syndrome 93.8 32 10319867 (1), 12181313 (1), 15702347 (1), 17115688 (1) (see all 29)
    rothmund-thomson syndrome 90.5 13 15702347 (1), 12595564 (1), 16793396 (1), 17623900 (1) (see all 13)
    progeria 75.9 4 12020873 (1), 17996922 (1), 17375009 (1), 10984715 (1)
    genetic disorder 65.8 11 9223443 (1), 11717307 (1), 12595564 (1), 16798775 (1) (see all 10)
    skin abnormalities 58.9 2 19238688 (1)
    cancer 56.5 61 16723399 (3), 19487340 (2), 10506209 (2), 14657658 (1) (see all 45)
    nijmegen breakage syndrome 55.4 2 15733840 (1), 11733219 (1)
    microsatellite instability 45.5 1 18084250 (2), 19470733 (1)
    cataract 43.9 1 10554436 (1)

    Genatlas disease: WRN
    Werner syndrome of premature ageing,with presenile subcapsular posterior cataract,associated with an increased
    risk of cancer and a chromosome instability

    GeneTests: WRN
    GeneReviews: WRN
    Genetic Association Database (GAD): WRN
    Human Genome Epidemiology (HuGE) Navigator: WRN (48 documents)

    Export disorders for WRN gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for WRN gene, integrated from 10 sources (see all 432):
    (articles sorted by number of sources associating them with WRN)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Association of a polymorphic variant of the Werner helicase gene with myocardial infarction in a Japanese population. (PubMed id 9021029)1, 2, 4 Ye L.... Ogihara T. (Am. J. Med. Genet. 1997)
    2. WRN polymorphisms affect expression levels of plasminogen activator inhibitor type 1 in cultured fibroblasts. (PubMed id 18312663)1, 4, 9 Castro E....Angel-ChA!vez L.I. (BMC Cardiovasc Disord 2008)
    3. Genetic variation in the premature aging gene WRN: a case-control study on breast cancer susceptibility. (PubMed id 17301258)1, 4, 9 Ding S.L....Shen C.Y. (amp 2007)
    4. Nucleolar localization of the Werner syndrome protein in human cells. (PubMed id 9618508)1, 2, 9 Marciniak R.A.... Guarente L. (Proc. Natl. Acad. Sci. U.S.A. 1998)
    5. Epigenetic inactivation of the premature aging Werner syndrome gene in human cancer. (PubMed id 16723399)1, 2, 9 Agrelo R.... Esteller M. (Proc. Natl. Acad. Sci. U.S.A. 2006)
    6. Werner protein is a target of DNA-dependent protein kinase in vivo and in vitro, and its catalytic activities are regulated by phosphorylation. (PubMed id 11889123)1, 2, 9 Karmakar P.... Bohr V.A. (J. Biol. Chem. 2002)
    7. The Werner syndrome protein binds replication fork and Holliday junction DNAs as an oligomer. (PubMed id 18596042)1, 2, 9 Compton S.A.... Griffith J.D. (J. Biol. Chem. 2008)
    8. Werner syndrome protein interacts functionally with translesion DNA polymerases. (PubMed id 17563354)1, 2, 9 Kamath-Loeb A.S.... Loeb L.A. (Proc. Natl. Acad. Sci. U.S.A. 2007)
    9. Solution structure of a multifunctional DNA- and protein-binding motif of human Werner syndrome protein. (PubMed id 16339893)1, 2, 9 Hu J.S.... Xi X.G. (Proc. Natl. Acad. Sci. U.S.A. 2005)
    10. Crystal structure of the HRDC domain of human Werner syndrome protein, WRN. (PubMed id 17148451)1, 2, 9 Kitano K.... Hakoshima T. (J. Biol. Chem. 2007)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 7486 HGNC: 12791 AceView: WRN Ensembl:ENSG00000165392 euGenes: HUgn7486
    ECgene: WRN H-InvDB: WRN

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for WRN Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for WRN Genetics and Cytogenetics in Oncology and Haematology
    WRNhttp://www.pathology.washington.edu/werner/ws_wrn.html
    GeneReviewshttp://www.ncbi.nlm.nih.gov/books/NBK1116/?term=WRN[genesymbol]
    NIEHS-SNPshttp://egp.gs.washington.edu/data/wrn/

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for WRN gene:
    Search GeneIP for patents involving WRN

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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