WRB Gene
protein-coding GIFtS: 50
GCID: GC21P040752
|
|
tryptophan rich basic protein
| |
Aliases
for WRB gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
10fRNAdb, and/or 11Nature:405,311-319 and CroW21)
About This Section
|
| Aliases |
|---|
| Tryptophan Rich Basic Protein1 2 | | Tryptophan-Rich Protein, Congenital Heart Disease 5 Protein11 | | CHD51 2 3 5 | | Tail-Anchored Protein Insertion Receptor WRB2 | | Congenital Heart Disease 5 Protein2 3 | | Tryptophan-Rich Protein2 | | Tryptophan-Rich Basic Protein2 3 | | |
Export aliases for WRB gene to outside databasesPrevious GC identifers: GC21P037326 GC21P039673 GC21P039672 GC21P039674 GC21P026220 |
Summaries
for WRB gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for WRB:
This gene encodes a basic nuclear protein of unknown function. The gene is widely expressed in adult and fetal tissues.Since the region proposed to contain the gene(s) for congenital heart disease (CHD) in Down syndrome (DS) patients hasbeen restricted to 21q22.2-22.3, this gene, which maps to 21q22.3, has a potential role in the pathogenesis of Downsyndrome congenital heart disease. Alternatively spliced transcript variants encoding different isoforms have beenfound for this gene. (provided by RefSeq, Apr 2009)
UniProtKB/Swiss-Prot: WRB_HUMAN, O00258Function: Receptor for ASNA1/TRC40-mediated insertion of tail-anchored (TA) proteins into the ER membrane
|
Genomic Views
for WRB gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics, Whole Chromsome Sequence According to
Nature (Cited Here with Permission):405,311-319 and CroW21)
About This Section
|
| GC21P040752: |
GeneLoc |
Nature:405,311-319 |
Start:
|
40,752,170 bp from pter |
26,325,991 bp from centromere |
End:
|
40,800,454 bp from pter |
26,343,350 bp from centromere |
Size:
|
48,285 bases |
17,360 bases |
Orientation:
|
plus strand |
plus strand |
| Whole chromosome sequencing: |
| cDNA sequence: |
Y12478 | | genomic clones: |
P128M19 |
Proteins
for WRB gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: WRB_HUMAN, O00258 (See
protein sequence)Recommended Name: Tail-anchored protein insertion receptor WRB Size: 174 amino acids; 19780 Da
Subunit: Interacts with ASNA1/TRC40
Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein
Secondary accessions: A8KAP8 A8MQ44 D3DSH9 O60740Alternative splicing: 2 isoforms: O00258-1 O00258-2 (No experimental confirmation available)Explore the universe of human proteins at neXtProt for WRB: NX_O00258
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_O00258
WRB Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins (2 alternative transcripts):
NP_001139690.1 NP_004618.2
ENSEMBL proteins: ENSP00000327716 ENSP00000381737 ENSP00000409030 ENSP00000370089 ENSP00000370084 ENSP00000410228 ENSP00000398295 ENSP00000420126 ENSP00000417590 ENSP00000445363
Human Recombinant Protein Products:
Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view): About this table
WRB for ontologies About GeneDecksing
WRB Antibody Products:
Assay Products for WRB: |
Protein
Domains /
Families
for WRB gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
WRB for domains About GeneDecksing
1 InterPro domain/family:Graphical View of Domain Structure for InterPro Entry O00258ProtoNet protein and cluster: O00258 UniProtKB/Swiss-Prot: WRB_HUMAN, O00258Similarity: Belongs to the WRB/GET1 family |
Function
for WRB gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: WRB_HUMAN, O00258Function: Receptor for ASNA1/TRC40-mediated insertion of tail-anchored (TA) proteins into the ER membrane Genatlas biochemistry entry for WRB:tryptophan-rich basic protein,with two copies in the same region or a gene and a pseudogene,expressed in fibroblastderived from fetal endocardial structures,potential transcriptional factor
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for WRB (see all 4)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for WRB (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
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Synthesis Service for WRB | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat WRB |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for WRB |
2 GenomeRNAi human phenotypes for WRB:
|
Pathways & Interactions
for WRB gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
 Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for WRB
STRING Interaction
Network Preview (showing 1 interactants - click image to see more details)
1 Interacting protein for WRB (ENSP000003277164) via UniProtKB, MINT, STRING, and/or I2DAbout this table
|
Drugs & Compounds
for WRB gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
WRB for compounds About GeneDecksing
 Browse Tocris compounds for WRB
10 Novoseek chemical compound relationships for WRB gene About this table
Search CenterWatch for drugs/clinical trials and news about WRB
|
Transcripts
for WRB gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for WRB gene (2 alternative transcripts): NM_001146218.1 NM_004627.4 Unigene Cluster for WRB: Tryptophan rich basic protein Hs.198308 [show with all ESTs]Unigene Representative Sequence: NM_00462717 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000333781(uc002yxs.3) ENST00000487869(uc011aep.1) ENST00000471468
ENST00000398753 ENST00000442773 ENST00000380713 ENST00000380708(uc002yxt.4 uc010goj.3)
ENST00000466787(uc011aeo.1) ENST00000490860 ENST00000415847 ENST00000417111
ENST00000488295 ENST00000471225 ENST00000478273 ENST00000476914 ENST00000480690
ENST00000541890
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for WRB (see all 4)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for WRB (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector (see all 2): WRB (NM_004627) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for WRB | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat WRB |
Additional cDNA sequence: AK293113.1 AK297186.1 AK299144.1 AK299245.1 AK310268.1 BC012415.1 BC111393.1 Y12478.1 18 DOTS entries: DT.100784071 DT.317368 DT.97846232 DT.121139444 DT.121139445 DT.100650176 DT.100784072 DT.95153764 DT.92445629 DT.121139419 DT.91747501 DT.121139478 DT.92445622 DT.95147285 DT.97785365 DT.97846231 DT.204294 DT.91895956 24/393 AceView cDNA sequences (see all 393): BE673753 AI401072 NM_007341 BM128577 AA687449 NM_001001713 CB114295 BU734633 AV653124 CB113614 AA099383 BM703010 AI696477 BM698634 CA391782 BX092195 AI074642 BM128409 AU142922 AI699511 CA419722 BQ771788 BM470417 BP368925
GeneLoc Exon Structure
5/9 Alternative Splicing Database (ASD) splice patterns (SP) for WRB (see all 9) About this scheme
| ExUns: | 1a | · | 1b | · | 1c | · | 1d | · | 1e | · | 1f | ^ | 2a | · | 2b | ^ | 3a | · | 3b | · | 3c | · | 3d | ^ | 4 | ^ | 5a | · | 5b | · | 5c | ^ | 6a | · | 6b | ^ | 7a | · | 7b | · | 7c | · | 7d | · | 7e |
|---|
|
| SP1: | | | | | | | | | | | | | | | | | | | | | - | | - | | - | | | | | | - | | | | | | | | | | | | | | | | |
| SP2: | | | | | | | | | - | | - | | - | | - | | | | | | - | | - | | - | | | | | | - | | | | | | | | | | | | | | | | |
| SP3: | | | | | | | | | | | - | | - | | - | | | | | | - | | - | | - | | | | | | - | | | | | | | | | | | | | | | | |
| SP4: | | | | | | | | | | | | | | | | | | | | | - | | - | | - | | | | - | | - | | - | | | | | | | | | | | | | | |
| SP5: | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | - | | | | | | | | | | | | | | | |
ECgene alternative splicing isoforms for WRB
|
Expression
for WRB gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| WRB expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: ATGCATTGTT
About this image
See WRB Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for WRB
SOURCE GeneReport for Unigene cluster: Hs.198308
SABiosciences Custom PCR Arrays for WRB
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for WRB
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse, rat WRB | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat WRB | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat WRB | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for WRB |
Orthologs
for WRB gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of chordates.
Orthologs for WRB gene from 5/17 species (see all 17) About this table
ENSEMBL Gene Tree for WRB (if available)
TreeFam Gene Tree for WRB (if available) |
Paralogs
for WRB gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for WRB gene
WRB for paralogs About GeneDecksing
1 Pseudogenes.org Pseudogene for WRB
PGOHUM00000250729
|
Genomic Variants
for WRB gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 21 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for WRB (40752170 - 40800454 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for WRB: -- | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing WRB |
|
Disorders
/ Diseases
for WRB gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
WRB for disorders About GeneDecksing
OMIM gene information: 602915
OMIM disorders: --
5  diseases for WRB: About MalaCardsdown syndrome autoimmune hemolytic anemia hemolytic anemia anxiety disorder
anemia
2 diseases from the University of Copenhagen DISEASES database for WRB:Autoimmune hemolytic anemia Down syndrome Human Genome Epidemiology (HuGE) Navigator: WRB (1 document)
Export disorders for WRB gene to outside databases
|
Publications
for WRB gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for WRB gene, integrated from 9 sources (see all 57):
(articles sorted by number of sources associating them with WRB) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Identification and characterization of a new human cDNA from chromosome 21q22.3 encoding a basic nuclear protein. (PubMed id 9544840)1, 2, 3, 9 Egeo A.... Scartezzini P. (1998)
- WRB is the receptor for TRC40/Asna1-mediated insertion of tail- anchored proteins into the ER membrane. (PubMed id 21444755)1, 2 Vilardi F.... Dobberstein B. (2011)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- The DNA sequence of human chromosome 21. (PubMed id 10830953)1, 2 Hattori M....Yaspo M.L. (2000)
- Molecular machinery for insertion of tail-anchored mem brane proteins into the endoplasmic reticulum membrane in mammalian cells. (PubMed id 23041287)1 Yamamoto Y. and Sakisaka T. (2012)
- Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)
- Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (2011)
- Replication of previous genome-wide association studi es of bone mineral density in premenopausal American women. (PubMed id 20200978)1 Ichikawa S....Econs M.J. (2010)
- Toward a confocal subcellular atlas of the human proteome. (PubMed id 18029348)1 Barbe L....Andersson-Svahn H. (2008)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)2 Ota T.... Sugano S. (2004)
|
External Searches for WRB gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing WRB gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing WRB gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing WRB gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for WRB | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for WRB gene:
Search GeneIP for patents involving WRB
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for WRB gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for WRB | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for WRB | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for WRB | | | OriGene Protein Over-expression Lysate for WRB | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for WRB | | OriGene 3'-UTR Clone for WRB | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for WRB | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for WRB | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | Browse OriGene full length recombinant human proteins expressed in human HEK293 cells | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for WRB | | OriGene Custom Protein Services for WRB | | | OriGene Custom Immunoassay Development | | |
| |
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat WRB | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing WRB | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat WRB | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat WRB | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat WRB | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat WRB |
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 |  |  |  | |
| | | | Search Tocris compounds for WRB |
| |  |  |  |  | | | | |
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 | | | WRB Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for WRB |
|  |  |  | | | | ThermoFisher Antibodies for WRB |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat WRB |
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